RESUMEN
In this paper, Shannong 87074-519, a derivative of wheat-decaploid Elytrigia elongata, was identified by inoculation assessment, cytological analysis, simple sequence repeat (SSR), molecular marker technique,and genomic in situ hybridization (GISH). The results are as follows: the chromosome number of Shannong87074-519 in root tip cells was 2n=44, 22 bivalents were observed in most PMC at MI, and the average chromosome configuration was 2n=44=21.82 II+0.36 I , and the chromosome configuration (2n=43=21 II +1 I) was observed in most PMC of F1 between Shannong87074-519 and C.S. at MI. Therefore, it was an alien disomic addition line with one pair chromosome of Elytrigia elongata. Then the St total genomic DNA was labeled as probe in GISH, the green-yellow hybridization signal was observed in two intact chromosomes, indicating that Shannong87074-519 was added by one pair chromosome of St genome. The SSR-PCR technique was employed in the primer filtration, and the molecular marker BARC165 was singled out from 170 primers, which could amplify the specific molecular marker BARC165(268) of Elytrigia elongata in Shannong87074-519. Subsequently, the specific segment in Elytrigia elongata was cloned and labeled as probe in GISH of root tip cells of Shannong87074-519, the light yellow hybridization signal was observed in both chromatin at interphase and chromosome at mitotic metaphase, thus the BARC165(268) could be applied as a specific molecular marker to detect alien chromatin of Elytrigia elongata in Shannong87074-519. Because of the good agronomic characteristics, high immunity to yellow rust, and dominant new yellow rust resistant gene located at the added chromosome St, assigned as YrSt temporarily, Shannong87074-519 has very important value in wheat breeding and genetics improvement.
Asunto(s)
Quimera/inmunología , Hibridación Genética , Inmunidad Innata , Enfermedades de las Plantas/inmunología , Poaceae/genética , Poliploidía , Triticum/genética , Basidiomycota/fisiología , Quimera/genética , Cromosomas de las Plantas/genética , Hibridación Fluorescente in Situ , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/genética , Triticum/inmunologíaRESUMEN
6 alien disomic addition lines (line0605; line0607, line0609, line0610, line0611, line0625), which were selected from 59 wheat-Elytrigia intermedium hybridization germ plasm lines, were identified by using the methods of morphology, powdery mildew resistance identifications, cytology and RAPD analysis. Morphogy results showed that they all had the better agronomic characters of their parents; Cytogic results showed that the chromosome configuration at PMC M I was 2n = 22 II; 209 random primers were used for RAPD amplification, result showed 5 of them amplified the specific bands of Elytrigia intermedium parents of 6 disomic addition lines, which could be used as RAPD markers for alien addition chromosomes. Results of powdery mildew resistance showed that line0605 was immune, line0607 was intermediately resistant, line 0610 and line0625 were highly resistant, and line0609 and line0611 were intermediately sensitive.