Enhancing Mechanical and Antimicrobial Properties of Dialdehyde Cellulose-Silver Nanoparticle Composites through Ammoniated Nanocellulose Modification.

Given the widespread prevalence of viruses, there is an escalating demand for antimicrobial composites. Although the composite of dialdehyde cellulose and silver nanoparticles (DAC@Ag1) exhibits excellent antibacterial properties, its weak mechanical characteristics hinder its practical applicability. To address this limitation, cellulose nanofibers (CNFs) were initially ammoniated to yield N-CNF, which was subsequently incorporated into DAC@Ag1 as an enhancer, forming DAC@Ag1/N-CNF. We systematically investigated the optimal amount of N-CNF and characterized the DAC@Ag1/N-CNF using FT-IR, XPS, and XRD analyses to evaluate its additional properties. Notably, the optimal mass ratio of N-CNF to DAC@Ag1 was found to be 5:5, resulting in a substantial enhancement in mechanical properties, with a 139.8% increase in tensile elongation and a 33.1% increase in strength, reaching 10% and 125.24 MPa, respectively, compared to DAC@Ag1 alone. Furthermore, the inhibition zones against Escherichia coli and Staphylococcus aureus were significantly expanded to 7.9 mm and 15.9 mm, respectively, surpassing those of DAC@Ag1 alone by 154.8% and 467.9%, indicating remarkable improvements in antimicrobial efficacy. Mechanism analysis highlighted synergistic effects from chemical covalent bonding and hydrogen bonding in the DAC@Ag1/N-CNF, enhancing the mechanical and antimicrobial properties significantly. The addition of N-CNF markedly augmented the properties of the composite film, thereby facilitating its broader application in the antimicrobial field.

Senolytic Therapy Enabled by Senescent Cell-Sensitive Biomimetic Melanin Nano-senolytics.

Cellular senescence is a significant risk factor for aging and age-related diseases (ARD). The canonical senolytics Dasatinib and Quercetin (DQ) have shown promise in clearing senescent cells (SnCs); however, the lack of selectivity poses a challenge in achieving optimal outcomes. Despite the recent occurrence of the nanomaterial-based approaches targeting SnCs, limited therapeutic effects and potential toxicity still remain a major concern. Herein, we developed a "double locks-like" nanoplatform that integrated Galactan coating and mesoporous polydopamine to encase the senolytic drug DQ. By this way, DQ was only released in SnCs that were featured with higher levels of ß-galactosidase (ß-gal) and low PH. Additionally, the nanoparticles were equipped with 2,2,6,6-Tetramethylpiperidine-1-oxyl (Tempo) to gain enhanced photothermal converting potential. Consequently, the synthesized nanosenolytics demonstrated remarkable specificity and efficacy in eradicating SnCs, and accordingly reversed pulmonary fibrosis in mice without affecting normal tissues. Upon exposure of near-infrared (NIR) light, the nanoparticles demonstrated to efficiently remove senescent tumor cells inducted by chemotherapy, thereby hindering the outgrowth and metastasis or breast cancer. Collectively, the present study develops an "On/Off" switchable nanoplatform in response to SnCs, and produces a more safe, efficient and feasible way to delay aging or alleviate age-associated diseases. This article is protected by copyright. All rights reserved.

Characteristics of Dialdehyde Cellulose Nanofibrils Derived from Cotton Linter Fibers and Wood Fibers.

Two types of cellulose nanofibrils (CNFs) were isolated from cotton linter fibers and hardwood fibers through mechanical fibrillation methods. The dialdehyde cellulose nanofibrils (DACNFs) were prepared through the periodate oxidation method, and their morphological and structural properties were investigated. The characteristics of the DACNFs during the concentration process were also explored. The AFM analysis results showed that the mean diameters of wood fiber-based CNFs and cotton fiber-based CNFs were about 52.03 nm and 69.51 nm, respectively. However, the periodate oxidation treatment process obviously reduced the nanofibril size and destroyed the crystalline region of the nanofibrils. Due to the high crystallinity of cotton fibers, the cotton fiber-based DACNFs exhibited a lower aldehyde content and suspension stability compared to the wood fiber-based DACNFs. For the concentration process of the DACNF suspension, the bound water content of the concentrated cotton fiber-based DACNFs was lowered to 0.41 g/g, which indicated that the cotton fiber-based DACNFs could have good redispersibility. Both the wood fiber-based and cotton fiber-based DACNF films showed relatively good transmittance and mechanical strength. In addition, to the cotton fiber-based DACNF films had a very low swelling ratio, and the barrier water vapor and oxygen properties of the redispersed cotton fiber-based DACNF films decreased by very little. In sum, this study has demonstrated that cotton fibers could serve as an effective alternative to wood fibers for preparing CNFs, and that cotton fiber-based DACNFs have huge application prospects in the field of packaging film materials due to their stable properties during the concentration process.

Water-soluble silver nanoclusters with multicolor fluorescence generated by dialdehyde nanofibrillated cellulose for biological imaging.

Water-soluble silver nanoclusters (AgNCs) as a new type of fluorescent material have attracted much attention for their remarkable optical properties and excellent cytocompatibility. However, it is still challenging to synthesize water-soluble AgNCs with good cytocompatibility and excellent fluorescence. Herein, the dialdehyde nanofibrillated cellulose (DANFC)- reduced water-soluble AgNCs capped by glutathione (GSH) with tunable fluorescence emissions were first reported. The DANFC provides a mild reduction environment and crystal growth system for the coordination between silver ions and GSH compared to conventional methods using strong reducing agents. The AgNCs with intense red fluorescence (R-AgNCs@GSH, size ∼2.24 nm) and green fluorescence (G-AgNCs@GSH, size ∼1.93 nm) were produced by varying the ratios of silver sources and ligands, and could maintain stable fluorescence intensity over 6 months. Moreover, the CCK-8 study demonstrated that the R-AgNCs@GSH and G-AgNCs@GSH reduced by DANFC of excellent cytocompatibility (cell viability >90 %) and enable precise multicolor intracellular imaging of Hela cells in 1 h. This work proposes a novel method to synthesize water-soluble AgNCs with tunable fluorescence emission at room temperature based on the classical silver- mirror reaction (SMR) using DANFC as reducing agent, and the synthesized fluorescent AgNCs have great potential as novel luminescent nanomaterials in biological research.

Defective Lamtor5 Leads to Autoimmunity by Deregulating v-ATPase and Lysosomal Acidification.

Despite accumulating evidence linking defective lysosome function with autoimmune diseases, how the catabolic machinery is regulated to maintain immune homeostasis remains unknown. Late endosomal/lysosomal adaptor, MAPK and mTOR activator 5 (Lamtor5) is a subunit of the Ragulator mediating mechanistic target of rapamycin complex 1 (mTORC1) activation in response to amino acids, but its action mode and physiological role are still unclear. Here it is demonstrated that Lamtor5 level is markedly decreased in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE). In parallel, the mice with myeloid Lamtor5 ablation developed SLE-like manifestation. Impaired lysosomal function and aberrant activation of mTORC1 are evidenced in Lamtor5 deficient macrophages and PBMCs of SLE patients, accompanied by blunted autolysosomal pathway and undesirable inflammatory responses. Mechanistically, it is shown that Lamtor5 is physically associated with ATP6V1A, an essential subunit of vacuolar H+-ATPase (v-ATPase), and promoted the V0/V1 holoenzyme assembly to facilitate lysosome acidification. The binding of Lamtor5 to v-ATPase affected the lysosomal tethering of Rag GTPase and weakened its interaction with mTORC1 for activation. Overall, Lamtor5 is identified as a critical factor for immune homeostasis by intergrading v-ATPase activity, lysosome function, and mTOR pathway. The findings provide a potential therapeutic target for SLE and/or other autoimmune diseases.

Acid-catalyzed phenolation of lignin with tea polyphenol: Enhancing uv resistance and oxidation resistance for potential applications.

The rapid development of the industry has led to the destruction of the earth's ozone layer, resulting in an increasingly serious problem of excessive ultraviolet radiation. Exploring effective measures to address these problems has become a hot topic. Lignin shows promise in the design and preparation of anti-ultraviolet products due to its inherent properties. However, it is important to investigate way to enhance the reactivity of lignin and determine its application form in related products. In this study, phenolic reactions with tea polyphenols were conducted through acid-catalyzed conversion, utilizing organic solvent lignin as the primary material. The phenolic hydroxyl content of the original lignin increased significantly by 218.8 %, resulting in notable improvements in UV resistance and oxidation resistance for phenolic lignin. Additionally, micro-nanocapsule emulsions were formed using phenolic lignin particles as surfactants through ultrasonic cavitation with small-molecule sunscreens. A bio-based sunscreen was prepared with phenolated lignin micro-nanocapsules as the active ingredient, achieving an SPF 100.2 and demonstrating excellent stability. The sunscreen also exhibited strong antioxidant properties and impermeability, ensuring user safety. This research offers a current solution for improving the application of lignin in sunscreens while also broadening the potential uses of plant-based materials in advanced functional products.

Development of high-barrier composite films for sustainable reduction of non-biodegradable materials in food packaging application.

Widely employed petroleum-based food packaging materials have inflicted irreparable harm on ecosystems, primarily stemming from their non-biodegradable attributes and recycling complexities. Inspired by natural nacre with a layered aragonite platelet/nanofiber/protein multi-structure, we prepared high-barrier composite films by self-assembly of cellulose nanofibrils (CNF), cellulose nanocrystals (CNC), montmorillonite (MMT), polyvinyl alcohol (PVA) and alkyl ketene dimer (AKD). The composite films demonstrated outstanding barrier properties with oxygen vapor transmission of 0.193 g·mm·m-2·day-1 and water vapor transmission rates of 0.062 cm3·mm·m-2·day-1·0.1 MPa-1, which were significantly lower than those of most biomass-degradable packaging materials. Additionally, the impacts of mixing nanocellulose with various aspect ratios on the tensile strength and folding cycles of the films were examined. The exceptional resistance of the composite films to oil and water provides a novel and sustainable approach to reduce non-biodegradable plastic packaging.

Skin healthcare protection with antioxidant and anti-melanogenesis activity of polysaccharide purification from Bletilla striata.

In this study, we investigated the structural characterization and biological activities of Bletilla striata polysaccharides (BSPs) for their role as antioxidants and anti-melanogenesis agents in skin healthcare protection. Three neutral polysaccharides (BSP-1, BSP-2, and BSP-3) with molecular weights of 269.121 kDa, 57.389 kDa, and 28.153 kDa were extracted and purified. Their structural characteristics were analyzed by ion chromatography, GC-MS, and 1D/2D NMR. The results showed that BSP-1, which constitutes the major part of BSPs, was composed of α-D-Glcp, ß-D-Glcp, ß-D-Manp, and 2-O-acetyl-ß-D-Manp, with the branched-chain accompanied by ß-D-Galp and α-D-Glcp. BSP-1, BSP-2, and BSP-3 can enhance the total antioxidant capacity of skin fibroblasts with non-toxicity. Meanwhile, BSP-1, BSP-2, and BSP-3 could significantly inhibit the proliferative activity of melanoma cells. Among them, BSP-1 and BSP-2 showed more significance in anti-melanogenesis, tyrosinase inhibition activity, and cell migration inhibition. BSPs have effective antioxidant capacity and anti-melanogenesis effects, which should be further emphasized and developed as skin protection components.

Distribution and source of heavy metals in Tibetan Plateau topsoil: New insight into the influence of long-range transported sources to the surrounding glaciers.

Heavy metals present a substantial threat to both the environment and human health. Considering the delicate ecological equilibrium of the Tibetan Plateau (TP) and its heightened susceptibility to anthropogenic impacts, scholarly attention has progressively turned toward the examination of heavy metal pollution within the plateau's environment. In this study, we conducted a comprehensive analysis of various heavy metals (As, Cr, Co, Ni, Cu, Mo, Cd, Pb, and Sb), utilizing topsoil samples collected from the TP during the period of 2018-2021. Additionally, snow and cryoconite samples obtained from TP glaciers during the same timeframe were also subjected to analysis. The results indicate elevated concentrations of total heavy metals in the eastern and western TP (328.7 µg/g), as opposed to the central and southern TP (145.7 µg/g). Most heavy metals exhibit a consistent spatial distribution pattern. High Enrichment Factors (EFs) and Geoaccumulation Index (Igeo) values for As and Cd suggest their enrichment in TP topsoil. Receptor modeling identified three primary sources of heavy metals within the topsoil: industrial sources (42.3%), inherent natural sources within the surface soil (20.6%), and vehicular emissions (14.2%). Substantial differences in heavy metal concentrations and spatial distribution were observed between the topsoil and the glacial snow-cryoconite matrix. The prominent presence of Sb in the snow-cryoconite matrix, in contrast to its low abundance in the topsoil, indicates distinct source influences of long-range transported materials between the two environments. Our inference suggests that the influence of heavy metals from distant pollutants undergo mixing and dilution in the topsoil due to the presence of local indigenous heavy metals, although such influence is notably observed on the glacier surface of the TP. Consequently, this underscores the significant impact of long-range transported sources on heavy metals, surpassing the influence of local TP soils, to the alpine glaciers and even other atmospheric sediments in Tibetan Plateau.

Ultra-strong, nonfreezing, and flexible strain sensors enabled by biomass-based hydrogels through triple dynamic bond design.

Biomass-based hydrogels have displayed excellent potential in flexible strain sensors due to their adequacy, biocompatibility, nontoxic and degradability. Nevertheless, their inferior mechanical properties, particularly at cryogenic temperatures, impeded their extensive utilization. Herein, we reported a rationally designed strain sensor fabricated from a gelatin and cellulose-derived hydrogel with superior mechanical robustness, cryogenic endurance, and flexibility, owing to a triple dynamic bond strategy (TDBS), namely the synergistic reinforcement among potent hydrogen bonds, imine bonds, and sodium bonds. Beyond conventional sacrificing bonds consisting of hydrogen bonds, dynamic covalent bonds and coordinate bonds, synergetic triple dynamic bonds dominated by strong hydrogen bonds and assisted by imine and sodium bonds with higher strength can dissipate more mechanical energy endowing the hydrogel with 38-fold enhancement in tensile strength (6.4 MPa) and 39-fold improvement in toughness (2.9 MPa). We further demonstrated that this hydrogel can work as a robust and biodegradable strain sensor exhibiting remarkable flexibility, broad detection range, considerable sensitivity and excellent sensing stability. Furthermore, owing to the improved nonfreezing performance achieved from incorporating sodium salts, the sensor delivered outstanding sensing properties under subzero conditions such as -20 and -4 °C. It is anticipated that the TDBS can create diverse high-performance soft-electronics for broad applications in human-machine interfaces, energy and healthcare.

Final epidemic size and critical times for susceptible-infectious-recovered models with a generalized contact rate.

During the spread of an infectious disease, the contact rate or the incidence rate may affect disease characteristics. For simplicity, most disease models assume standard incidence or mass action rates to calculate the basic reproduction number, final epidemic size, and peak time of an epidemic. For standard incidence, the contact rate remains constant resulting in the incidence rate is inversely proportional to the population size, while for the mass action rate, this contact rate is proportional to the total population size resulting in the incidence rate is independent of the population size. In this paper, we consider susceptible-infectious-recovered epidemic models with a generalized contact rate C(N) and a nonlinear incidence rate in view of the behavioral change from susceptible or infectious individuals when an infectious disease appears. The basic reproduction number and the final size equation are derived. The impact of different types of contact rates on them is studied. Moreover, two critical times (peak time and epidemic duration) of an epidemic are considered. Explicit formulas for the peak time and epidemic duration are obtained. These formulas are helpful not only for taking early effective epidemic precautions but also for understanding how the epidemic duration can be changed by acting on the model parameters, especially when the epidemic model is used to make public health policy.

High-strength hydrophilic N-halamines chitosan and cellulose nanofibers membranes with repeated bactericidal properties.

Direct addition of disinfectants and membrane separation techniques have been common methods to address microbial contamination in water. However, disinfectants may generate toxic by-products, and even minor damage or biofilm formation on filtration membranes can lead to a heightened risk of microbial contamination. Consequently, how to quickly and safely disinfect microbial contaminated water sources remains a huge challenge. In this study, the high-strength broad-spectrum antibacterial CNF/CS composite membrane was fabricated by utilizing cellulose nanofibers (CNF) to reinforce the structure of chitosan (CS). The resulting CNF/CS composite membrane exhibits an impressive tensile strength of 148 MPa and boasts an active chlorine content of 5.29 %. Notably, even after undergoing 50 washing cycles and 10 repeated chlorination procedures, the structural integrity and high active chlorine content of the composite membrane remain preserved, validating its exceptional strength, stability, and chlorine rechargeability. Additionally, the CNF/CS antibacterial materials demonstrate remarkable attributes in terms of rapid sterilization, sustained and consistent release of active chlorine, and efficient inhibition of biofilm formation, demonstrating great potential in efficient, green, and safe sterilization.

Serum Lipidomic Fingerprints Encode Early Diagnosis and Staging of Lung Cancer on a Novel PbS/Au-Layered Substrate.

Lung cancer (LC) is a major cause of mortality among malignant tumors. Early diagnosis through lipidomic profiling can improve prognostic outcomes. In this study, a uniform PbS/Au-layered substrate that enhances the laser desorption/ionization process, an interfacial process triggered on the substrate surface upon laser excitation, was designed to efficiently characterize the lipidomic profiles of LC patient serum. By controlling the stacking arrangement and particle sizes of PbS QDs and AuNPs, the optimized substrate promotes the generation of excited electrons and creates an enhanced electric field that polarizes analyte molecules, facilitating ion adduction formation ([M + Na]+ and [M + K]+) and enhancing detection sensitivity down to the femtomole level. Combining multivariate statistics and machine learning, a distinct lipidomic biomarker panel is successfully identified for the early diagnosis and staging of LC, with an accurate prediction validated by an area under the curve of 0.9479 and 0.9034, respectively. We also found that 18 biomarkers were significantly correlated with six metabolic pathways associated with LC. These results demonstrate the potential of this innovative PbS/Au-layered substrate as a sensitive platform for accurate diagnosis of LC and facilitate the development of lipidomic-based diagnostic tools for other cancers.

Sustainable and Scalable Synthesis of 2D Ultrathin Hierarchical Porous Carbon Nanosheets for High-Performance Supercapacitor.

2D carbon nanomaterials such as graphene, carbon nanosheets, and their derivatives, representing the emerging class of advanced multifunctional materials, have gained great research interest because of their extensive applications ranging from electrochemistry to catalysis. However, sustainable and scalable synthesis of 2D carbon nanosheets (CNs) with hierarchical architecture and irregular structure via a green and low-cost strategy remains a great challenge. Herein, prehydrolysis liquor (PHL), an industrial byproduct of the pulping industry, is first employed to synthesize CNs via a simple hydrothermal carbonization technique. After mild activation with NH4 Cl and FeCl3 , the as-prepared activated CNs (A-CN@NFe) display an ultrathin structure (≈3 nm) and a desirable specific surface area (1021 m2 g-1 ) with hierarchical porous structure, which enables it to be both electroactive materials and structural support materials in nanofibrillated cellulose/A-CN@NFe/polypyrrole (NCP) nanocomposite, and thus endowing nanocomposite with impressive capacitance properties of 2546.3 mF cm-2 at 1 mA cm-2 . Furthermore, the resultant all-solid-state symmetric supercapacitor delivers a satisfactory energy storage ability of 90.1 µWh cm-2 at 250.0 µW cm-2 . Thus, this work not only opens a new window for sustainable and scalable synthesis of CNs, but also offers a double profits strategy for energy storage and biorefinery industry.

Residual deformation of coal gangue subgrade filler under multi-vibration cyclic loading.

In this paper, the development law of residual deformation of coal gangue subgrade filler is analyzed through large scale triaxial test, and the residual deformation model of coal gangue mainly sandstone and limestone is established. The purpose is to provide research basis for the applicability of coal gangue as subgrade filler. The results show that the deformation of coal gangue filler increases first and then tends to be constant under cyclic load of multiple vibration times. It is found that the Shenzhujiang residual deformation model cannot accurately predict the deformation law, and the corresponding modification is made to the residual deformation model of coal gangue filling body. Finally, according to the calculation of grey correlation degree, the influence degree of main factors of coal gangue filler on its residual deformation is sorted. Combined with the actual engineering situation represented by these main factors, it can be analyzed that the effect of packing particle density on residual deformation is greater than that of packing particle size composition.

Distribution, composition and risk assessment of PAHs and PCBs in cryospheric watersheds of the eastern Tibetan Plateau.

Polycyclic Aromatic Hydrocarbons (PAHs) and Polychlorinated Biphenyls (PCBs) are significant components of persistent organic pollutants (POPs) and pose a threat to both ecosystems and human health. To explore their spatial distribution, origins, and risk assessment, we collected 25 glacial meltwater and downstream river water samples in the eastern Tibetan Plateau (including the Qilian Mountains in the northeast) during the summer of 2022 (June-July). Our results showed that ∑PAHs and ∑PCBs were present in a wide range from ND-1380 ng/L and ND-1421 ng/L, respectively. Compared to other studies worldwide, the ∑PAHs and ∑PCBs in the Hengduan Mountains were at high levels. The PAHs and PCBs mainly consisted of low-molecular-weight homologs, including Ace, Flu, Phe, and PCB52. Phe was the primary component of PAHs. Glacial meltwater samples generally exhibited low concentration of PAHs and PCB52, whereas downstream river water samples typically showed high concentration of PAHs and PCB52. We attributed this characteristic to the influence of pollutants physicochemical properties, altitude effect, long-range transport (LRT), and local environmental conditions. In the eastern Tibetan Plateau glacier basin (especially in the Hailuogou watersheds), the concentration of PAHs and PCB52 in runoff generally increased with decreasing elevation. We believe that the primary factor affecting the concentration of PAHs and PCB52 in the region is the difference in local human activity inputs from various altitudes. The composition characteristics of PAHs and PCBs suggested that incomplete coal combustion and coking discharge mainly caused PAHs, while the combustion of coal and charcoal and the release of capacitors primarily caused PCBs. We assessed the carcinogenic risk of PAHs and PCBs in the glacier basin of the TP and found that the potential threat of PAHs was stronger than that of PCBs. Overall, this study provides new insights into the ecological security of water resources in eastern Tibetan Plateau. It is significant for controlling PAHs and PCBs emissions, assessing the ecological environment of the glacier watershed, and regional human health.

Protocol to establish a genetically engineered mouse model of IDH1-mutant astrocytoma.

Lower-grade gliomas exhibit a high prevalence of isocitrate dehydrogenase 1 (IDH1) mutations, but faithful models for studying these tumors are lacking. Here, we present a protocol to establish a genetically engineered mouse (GEM) model of grade 3 astrocytoma driven by the Idh1R132H oncogene. We describe steps for breeding compound transgenic mice and intracranially delivering adeno-associated virus particles, followed by post-surgical surveillance via magnetic resonance imaging. This protocol enables the generation and use of a GEM to study lower-grade IDH-mutant gliomas. For complete details on the use and execution of this protocol, please refer to Shi et al. (2022).1.

An electrochemiluminescence immunosensor based on signal magnification of luminol using OER-activated NiFe2O4@C@CeO2/Au as effective co-reaction accelerator.

In this work, a novel, label-free electrochemiluminescence (ECL) immunosensor was constructed for the ultrasensitive detection of carbohydrate antigen 15-3 (CA15-3) by the combined use of NiFe2O4@C@CeO2/Au hexahedral microbox and luminol luminophore. The synthesis of the co-reaction accelerator (NiFe2O4@C@CeO2/Au) was related to the calcination of FeNi-based metal-organic framework (MOF), as well as the ingrowth of CeO2 nanoparticles and modification of Au nanoparticles. To be specific, the electrical conductivity will be boosted due to the Au nanoparticles, the synergetic effect generated between CeO2 and calcination FeNi-MOF could offer better activity of oxygen evolution reaction (OER). Herein, the NiFe2O4@C@CeO2/Au hexahedral microbox as a co-reaction accelerator has excellent OER activity and production of reactive oxygen species (ROS), thus increasing the ECL intensity of luminol in a neutral medium without other co-reactants such as H2O2. Because of these benefits, the constructed ECL immunosensor was applied to detect CA15-3 as an example under optimum conditions, the designed ECL immunosensor exhibited high-level selectivity and sensitivity for CA15-3 biomarker within a linear response range of 0.01-100 U mL-1 and an ultralow detection limit of 0.545 mU mL-1 (S/N = 3), demonstrating its potentially valuable application in the area of clinical analysis.

Mechanically strong nanopapers based on lignin containing cellulose micro- and nano-hybrid fibrils: Lignin content-fibrils morphology-strengthening mechanism.

Lignin-containing cellulose nanopapers are emerging multifunctional materials in the fields of coatings, films, and packaging. However, the forming mechanism and properties of nanopapers with various lignin content have not been thoroughly studied. In this work, a mechanically strong nanopaper was fabricated based on lignin-containing cellulose micro- and nano-hybrid fibrils (LCNFs). The influence of lignin content and fibrils morphology on the formation process of nanopapers was investigated to understand the strengthening mechanism of nanopapers. LCNFs with high lignin content provided nanopapers with intertwined micro- and nano-hybrid fibrils layers with small layer spacing, while LCNFs with low lignin content offered nanopapers interlaced nanofibrils layers with large layer spacing. Although lignin was expected to interfere with hydrogen bonds between fibrils, the uniformly distributed lignin contributed to the stress transfer between fibrils. Due to the good coordination between microfibrils, nanofibrils and lignin (as network skeleton, filler and natural binder, respectively), the well-designed LCNFs nanopapers with lignin content of 14.5 % showed excellent mechanical properties, including tensile strength (183.8 MPa), Young's modulus (5.6 GPa) and elongation (9.2 %). This work deeply reveals the relationship between lignin content, morphology and strengthening mechanism of nanopapers, and providing theoretical guidance for employing LCNFs as structural and reinforcing materials to design robust composites.

Novel design constructed In2S3@SnO2 hollow heterojunctions by insufficiently etched MOFs as framework for photoelectrochemical bioanalysis.

Compared to sufficiently etched MOFs materials, insufficiently etched MOFs materials tend to display unsatisfactory performance due to their immature structure and have been eliminated from scientific research. Herein, this work reported a novel In2S3@SnO2 heterojunction (In2S3@SnO2-HSHT) materials, which were stably synthesized in high temperature aqueous environment and equipped extraordinary photoelectrochemical (PEC) properties, fabricated by a succinct hydrothermal synthesis method using insufficiently etched MIL-68 as a self-sacrificing template. Compared with the control groups and In2S3@SnO2 heterojunctions with collapse morphology synthesized by sufficiently etched MIL-68 in high temperature aqueous environment, In2S3@SnO2-HSHT synthesized from insufficiently etched MIL-68 as a template had a massively enhanced light-harvesting capability and generated more photoinduced charge carriers due to its well-preserved hollow structure. Therefore, based on outstanding PEC performance of In2S3@SnO2-HSHT, the established PEC label-free signal-off immunosensor to detect CYFRA 21-1, revealing vivid selectivity, stability, and reproducibility. This novel strategy adopted the insufficient chemical etching method neglected by the mainstream chemical etching approaches, which solved the challenge that the stability of the sufficient etched MOFs with hollow structure cannot be maintained under the subsequent high temperature aqueous reaction conditions, and was further applied to the design of hollow heterojunction materials for photoelectrochemical fields.