Surgical management of iliac wing fractures: Proposal of a new classification system.

INTRODUCTION: Isolated fractures of the iliac wing are a rare injury, usually occurring in high-energy trauma, and are associated with other non-musculoskeletal and soft tissue injuries that could compromise the patient's life. Surgical indications are unclear, and there is limited information on the most frequent fracture patterns. METHODS: A descriptive multicenter case series study of isolated fractures of the iliac wing treated surgically in three referral trauma hospitals in Latin America. The different fracture patterns are described, the "iliac ring" concept is proposed, and a classification is made. RESULTS: Twenty-eight patients were included; 24 were male, the median age was 31 years RIQ (24-46), the most frequent trauma mechanism was a traffic accident, and in 14 patients, the ISS >16. The most frequent associated injury was to the appendicular skeleton at another level in 13 patients. In the new classification, according to the number of fragments, 11 patients were classified as type A (1 fragment), ten patients as type B (two fragments), and seven patients as type C (three or more fragments). The most compromised anatomical area was the crest and anterosuperior iliac spines in 26 patients, followed by the fossa and anteroinferior iliac spine in 17 and 8 patients, respectively. DISCUSSION: The patterns of isolated fractures of the iliac wing allow the identification of three types of fractures. Identifying these patterns can help the surgeon decide to perform surgery in these scenarios and choose the fixation technique according to the number and location of the fragments.

hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes.

Salmonella enterica is a foodborne pathogen that can be internalized into fresh produce. Most of the Salmonella virulence genes are clustered in regions denominated Salmonella Pathogenicity Islands (SPI). SPI-1 encodes a Type Three Secretion System (T3SS-1) and effector proteins that allow the internalization of Salmonella into animal cells. HilD is a transcriptional regulator that induces the expression of SPI-1 genes and other related virulence genes located outside of this island. Here, we assessed the role of hilD in the internalization of Salmonella Newport and Typhimurium into cherry tomatoes, by evaluating either an isolate from an avocado orchard, S. Newport-45 or the laboratory strain S. Typhimurium SL1344 and their isogenic mutants in hilD. The internalization of these bacteria was carried out by using a temperature gradient of 12°C. The transcription of hilD and invA was tested by qRT-PCR experiments. Our results show that S. Newport-45 hilD mutant viable cells obtained from the interior of the fruit were decreased (2.7-fold), compared with those observed for S. Typhimurium SL1344. Interestingly, at 3 days postinoculation, the cells recovered from S. Newport-45 hilD mutant were similar to those recovered from all the strains evaluated, suggesting that hilD is required only for the initial internalization of S. Newport.

Pulmonary cement embolism following transpedicular screws placement for thoracolumbar fractures.

BACKGROUND: Symptomatic pulmonary cement embolism in patients undergoing thoracic transpedicular fenestrated screw placement is rare. Here, we have added a 64-year-old female undergoing transpedicular screw placement for a T11 fracture who developed a pulmonary cement embolism intraoperatively and add this case to 13 others identified in the literature. CASE DESCRIPTION: A 64-year-old female presented with a type "C", ASIA "E" T11 fracture. The thoracolumbar pedicle screw fixation was supplemented with bone cement due to her underlying severe osteoporosis. During the fluoroscopy-guided supplementation with bone cement, a leak through the paravertebral venous system was noted. Thirty minutes later, the patient acutely developed extreme respiratory failure and required mechanical ventilation for the next 2 days. The diagnosis of pulmonary embolism due to bone cement was confirmed on a contrast computed tomography study of the chest. CONCLUSION: Symptomatic pulmonary cement embolization supplementing transpedicular screws placement for osteoporotic bone is rare. Here, we present a 64-year-old female who during transpedicular fixation of a T11 fracture developed an acute pulmonary embolism from the bone cement resulting in the need for 2 days of postoperative artificial ventilation.

Risoterapia: una terapia complementaria a la medicina occidental / Laughter therapy: a complementary therapy to western medicine / Risoterapia: uma terapia complementar à medicina ocidental

Resumen La enfermedad y la hospitalización son factores estresantes, las instituciones tienen el deber de manejar de forma integral la salud de los pacientes. Para esto existen múltiples terapias complementarias como la risoterapia. Estas se basan en el humor para la promoción de la salud y se encargan de divertir a las personas, llevando sonrisas donde predominan los descontentos. Sus beneficios son múltiples y sus contraindicaciones son pocas, lo que la convierte en una estrategia costo-efectiva para mejorar la calidad de vida durante la hospitalización. Con este artículo, se pretende, no solo mostrar los asuntos más relevantes en la risoterapia, sino también motivar a investigar, así como concientizar al lector de la importancia de apoyar este nuevo boom.

Abstract Disease and hospitalization are stressful factors, institutions have a duty to comprehensively manage patients' health. For this there are multiple complementary therapies such as laughter therapy. These are based on humor for health promotion and are responsible for entertaining people, bringing smiles where usually there is discontent. Its benefits are multiple and its contraindications are few, which makes it a cost-effective strategy to improve life quality during hospitalization. This article, aims not only to show the most relevant issues in laughter therapy, but also to motivate research, as well as to create awareness among readers of the importance of supporting this new boom.

Resumo A doença e a hospitalização são fatores estressantes; nesse sentido, as instituições têm o dever de lidar de forma integral com a saúde dos pacientes. Para isso, existem múltiplas terapias complementares como a risoterapia. Esta se baseia no humor para a promoção da saúde e encarrega-se de divertir as pessoas, levando sorrisos aonde predominam os descontentamentos. Seus benefícios são múltiplos e seus contraindicações são poucas, o que a torna uma estratégia custo-efetiva para melhorar a qualidade de vida durante a hospitalização. Com este artigo, pretende-se não só mostrar os assuntos mais relevantes na risoterapia, mas também motivar a pesquisar, bem como conscientizar o leitor da importância de apoiar esse novo boom.

Prevalencia de síntomas respiratorios y riesgo de obstrucción al flujo aéreo en ginebra - valle del cauca / Prevalence of respiratory symptoms and risk of obstruction to air flow in ginebra - valle del cauca / Prevalência de sintomas respiratórios e riscos de obstrução ao fluxo de aire em ginebra - valle del cauca

RESUMEN Antecedentes y Objetivo: La prevalência de los principales síntomas respiratorios en Ginebra (Valle del Cauca) es desconocida. Este estudio busca investigar la prevalencia de disnea, tos, sibilancias y expectoración en Ginebra, un municipio rural de Colombia. Métodos: Se realizó un estudio de corte transversal, con una estrategia de muestreo por conglomerados para tener una muestra representativa de adultos mayores de 40 años. Cada paciente fue entrevistado con dos cuestionarios, el cuestionario de síntomas respiratorios Ferris validado para el español y el cuestionario de función pulmonar (LFQ), de este último, un puntaje menor o igual a 18 puntos fue considerado como riesgo de obstrucción al flujo aéreo. El análisis de datos fue realizado con software estadístico, el tamaño de muestra fue de 115 sujetos (prevalencia 50%, precisión 10% y porcentaje de pérdida del 20%), las variables cuantitativas se resumieron en frecuencias y porcentajes y las variables cuantitativas en medianas y rangos intercuartílicos, la prevalencia de síntomas respiratorios se calculó con un intervalo de confianza del 95% y se realizaron cruces exploratorios entre las variables de síntomas respiratorios y antecedentes exposicionales. Resultados: 146 sujetos, 2 pérdidas, mediana de edad 60,5 años (RIQ:22), 52,1% hombres, prevalencia de tos 18,1% (IC95%:11,8-24,3), expectoración 20,1% (IC95%:13,9-27,1), sibilancias 28,2% (IC95%:21,1-35,9), disnea 33,3% (IC95%:25-41), personas a riesgo de limitación flujo aéreo 19,4% (IC95%:14,2-28,4). En la realización de cruces exploratorios se obtuvo una relación entre el número de paquetes año y la presencia de tos (p=0,008), antecedente de exposición a leña y tos (p=0,008), número de paquetes año y expectoración (p=0,018), sibilancias y consumo de cigarrillo (p=0,047), disnea y antecedente de enfermedad coronaria (p=0,028), disnea y antecede de hipertensión arterial (p=0,037). Conclusión: La disnea es el síntoma respiratorio más frecuente en pacientes mayores de 40 años en Ginebra (Valle del Cauca), el antecedente de tabaquismo se relacionó con tos y sibilancias. La utilización de un cuestionario de síntomas respiratorios puede ser útil para identificar pacientes con riesgo de obstrucción al flujo aéreo cuando se compara con el auto-reporte de enfermedad respiratoria.

ABSTRACT Background and objective: The prevalence of main respiratory symptoms in Ginebra (Valle del Cauca) is unknown. This study aimed to investigate dyspnea, cough, wheezes and expectoration in Ginebra a Town of Colombia. Methods: A cross-sectional design and a random, conglomerates-sampling strategy were used to provide representative samples of adults aged > 40 years. Each participant was interviewed with two questionnaires, validated Spanish version of the Ferris Respiratory Questionnaire and Lung Function Questionnaire (LFQ), of the latter, and a score less or equal 18 points was considered with risk of obstructive airflow. Analysis was performed using statistical software, sample size of 115 subjects (prevalence 50%, precision 10% and lost percentage 20%), the variables quantitative were summarized with frequencies and percentages, and the variables qualitative in Median and range interquartile, the prevalence of respiratory symptoms was calculated with CI95%, exploratory analysis were made with respiratory symptoms variables and exposure antecedents. Results: 146 participants, with 2 missing, Median of age 60,5 years (IQR: 22), 52,1% men, prevalence of cough 18,1% (CI95%: 11,8-24,3), expectoration 20,1% (IC95%:13,9-27,1), wheezes 28,2% (IC95%:21,1-35,9), dyspnea 33,3% (CI95%:25-41), subjects with risk of airflow obstruction 19,4% (CI95%:14,2-28,4). Exploratory analysis showed relationship between package-year index and cough (p=0,008), wood smoke exposure and cough (p=0,008), package-year index and expectoration (p=0,018), wheezes and smoking exposure (p=0,047), dyspnea and coronary disease (p=0,028), and dyspnea and arterial hypertension (p=0,037). Conclusion: Dyspnea is the respiratory symptom more frequent in Ginebra (Valle del Cauca), the antecedent of smoking was relationship with cough and wheezes. Using a questionnaire of respiratory symptoms can be useful to identify patients with risk of airflow obstruction, when is compared with the auto-report of respiratory disease.

RESUMO Antecedentes e objetivo: A prevalência de sintomas respiratórios principais em Ginebra (Valle del Cauca) é desconhecida. Este estudo teve como objetivo investigar a dispneia, tosse, sibilos e expectoração em Ginebra, uma cidade da Colômbia. Métodos: Um desenho transversal e uma estratégia aleatória de amostragem de conglomerados foram utilizados para fornecer amostras representativas de adultos com idade> 40 anos. Cada participante foi entrevistado com dois questionários, versão validada em espanhol do Questionário Respiratório Ferris e Questionário de Função Pulmonar (LFQ), sendo este último considerado menor ou igual a 18 pontos com risco de fluxo obstrutivo de ar. A análise foi realizada utilizando software estatístico, tamanho de amostra de 115 indivíduos (prevalência 50%, precisão 10% e porcentagem perdida de 20%), as variáveis quantitativas foram resumidas com freqüências e porcentagens, e as variáveis qualitativas na mediana e intervalo interquartil, a prevalência de Os sintomas respiratórios foram calculados com IC95%, foram realizadas análises exploratórias com variáveis de sintomas respiratórios e antecedentes de exposição. Resultados: 146 participantes, com 2 perdidos, médios de idade 60,5 anos (IQR: 22), 52,1% homens, prevalência de tosse 18,1% (CI95%: 11,8-24,3), expectoração 20, 1% (IC95%: 13,9-27,1), sibilos 28,2% (IC95%: 21,1-35,9), dispnéia 33,3% (CI95%: 25-41), indivíduos com risco de obstrução do fluxo aéreo 19,4% (CI95%: 14,2-28,4). A análise exploratória mostrou relação entre índice do ano da embalagem e tosse (p = 0,008), exposição à fumaça da madeira e tosse (p = 0,008), índice do ano da embalagem e expectoração (p = 0,018), sibilos e exposição ao tabagismo (p = 0,047) dispnéia e doença coronária (p = 0,028) e dispnéia e hipertensão arterial (p = 0,037). Conclusão: A disnea é o sintoma respiratório mais freqüente em Ginebra (Valle del Cauca), o antecedente do tabagismo foi relação com tosse e sibilância. O uso de um questionário de sintomas respiratórios pode ser útil para identificar pacientes com risco de obstrução do fluxo aéreo, quando comparados com o auto-relato de doenças respiratórias.

Globicatella sanguinis: REPORTE DE UN CASO DE MENINGITIS EN EL HOSPITAL MILITAR CENTRAL / Globicatella sanguinis: REPORT OF A CASE OF MENINGITIS IN THE CENTRAL MILITARY HOSPITAL / Relatório de um caso de meningitis no hospital militar central

Globicatella sanguinis es un microorganismo patógeno poco común, coco Gram positivo y catalasa negativo, aunque se aísla en ganado ovino, ha sido descrito como agente infeccioso causante de meningitis en seres humanos. La primera descripción de este patógeno fue realizada por Collins y colaboradores en el año de 1992, se debe resaltar, que antes de este suceso, parte de los casos reportados como meningitis por S. viridans, pudieron ser causados en realidad por G. sanguinis debido a la falta de identificación de este ultimo y a la gran similitud en cuanto a morfología y resultados de pruebas bioquímicas. Al respecto, desde la descripción original hace 20 años, la información disponible de G. sanguinis es escasa. Se ha presentado un caso de meningitis asociada a G. sanguinis posterior a un procedimiento quirúrgico en un paciente de 1 año de edad en el Hospital Militar Central.

Globicatella sanguinis is a rare pathogenic microorganism Gram-positive cocci and catalase negative, which al-though is isolated in sheep, has been described as infectious agent causing meningitis in humans. The first description of this pathogen was made by Colins et al. in 1992, it's necessary to outline that before this event, part of the cases reported as a meningitis cause by S. viridans could actually be caused by G. sanguinis due to the lack of identification of these ones and the great similarity in terms of morphology and results of biochemical tests. However, after 20 years of the original description, the available information of G. sanguinis is limited. A case of meningitis associated with G. sanguinis following a surgical procedure has been reported in a 1-year-old patient at the Central Military Hospital.

Globicatella sanguinis é um microrganismo patogênico raro, cocos grampositivos e catalase negativos, que emborasejam isolados em ovelhas, foram descritos como agentes infecciosos que causam meningite em seres humanos. A primeira descrição desse patógeno foi feita por Colins et al. Em 1992, é necessário esboçar que, antes deste evento, parte dos casos relatados como causa de meningitis por S. viridans poderia ser causada por G. sanguinis devido falta de identificação desses e à grande semelhança em termos de morfologia E resultados de testes bioquímicos. No entanto, após 20 anos da descrição original, a informação disponível de G. sanguinis é limitada. Um caso de meningite associado a G. sanguinis após um procedimento cirúrgico foi relatado em um paciente de 1 ano de idade no Hospital Militar Central.

Respiratory syncytial virus persistence in macrophages upregulates Fcgamma receptors expression.

Viruses can persist in differentiated cells (i.e., macrophages) over long periods of time, altering host cells functions but not inducing their death. We had previously reported that, in early passages (14-40) of a murine macrophage-like cell line persistently infected with respiratory syncytial virus (RSV) (MfP), FcgR-mediated phagocytosis and expression of FcgRIIB/RIII on the cell membrane were increased with respect to mock-infected macrophages (MfN). In this work, we explored the mechanism underlying such effects. Increases in FcgR expression and FcgR-mediated phagocytosis are preserved after more than 87 passages of the persistently infected culture. We analyzed the expression of FcgR isoforms at both mRNA and protein levels, and found out that RSV persistence distinctly affects the expression of FcgR isoforms. We also observed that the increase in FcgRs expression results neither from soluble factors (cytokines) or viral products released by the infected cells, nor from an increase in the rate of FcgR internalization. Our results suggest that RSV persistence in macrophages induce intracellular effects that have an impact on FcgRs gene expression at both mRNA and protein levels, and that the characteristics of RSV persistence were preserved for over 87 passages.

Estudio espectral de la estrategia postural de una población sana en pruebas estáticas / Spectrum analysis in postural strategy on static tests in a healthy population

Introducción y objetivos: La densidad espectral de potencia puede servir para encontrar comportamientos no evidentes en un sistema físico. Mediante la misma vamos a estudiar el comportamiento postural de una población sana, comparándolo con una situación de completa inmovilidad. Métodos: Un grupo de voluntarios sanos realizaron las pruebas correspondientes al modified Clinical Test for Sensory Interaction in Balance. El registro se hizo mediante un giroscopio de fibra óptica. Comparamos la densidad espectral de potencia de estas pruebas con otros registros donde el dispositivo está completamente inmóvil. Buscamos diferencias significativas mediante la prueba de ANOVA. Resultados: En las pruebas estáticas aparece un pico de potencia en 8,4Hz. El grupo estático presenta diferencias significativas con todas las pruebas y no aparecen cuando el factor diferenciador es la existencia o no de información visual. Sí las hay cuando la diferencia es la información somatosensorial. Conclusiones: Se necesitan pequeños movimientos para mantener la bipedestación. Estos movimientos se caracterizan por una frecuencia fundamental de 1,4Hz y un armónico a 8,4Hz. Las aferencias somatosensoriales son fundamentales para el mantenimiento del equilibrio (AU)

Introduction and objectives: The power spectral density can be used to find a hidden behaviour in a physical system. We studied postural behaviour in a healthy population, by means of power spectral density, and compared it with a situation of complete immobility. Methods: A group of healthy volunteers carried out the modified Clinical Test for Sensory Interaction in Balance. A fibre optic gyroscope device was used to record the data. We compared the power spectral density in these tests and with another recording where the device was motionless. We looked for significant differences using the ANOVA test. Results: A power peak appeared at 8.4Hz in all static tests. The static group showed significant differences with all the other tests. We found no statistically significant differences when visual input discriminated between 2 tests. There were statistically significant differences for the proprioceptive input. Conclusions: Brief movements are needed to maintain the quiet stance. The distinctive feature of these movements is a fundamental frequency at 1.4Hz and a harmonic frequency at 8.4Hz. Proprioceptive input is essential for balance (AU)

Spectrum analysis in postural strategy on static tests in a healthy population.

INTRODUCTION AND OBJECTIVES: The power spectral density can be used to find a hidden behaviour in a physical system. We studied postural behaviour in a healthy population, by means of power spectral density, and compared it with a situation of complete immobility. METHODS: A group of healthy volunteers carried out the modified Clinical Test for Sensory Interaction in Balance. A fibre optic gyroscope device was used to record the data. We compared the power spectral density in these tests and with another recording where the device was motionless. We looked for significant differences using the ANOVA test. RESULTS: A power peak appeared at 8.4 Hz in all static tests. The static group showed significant differences with all the other tests. We found no statistically significant differences when visual input discriminated between 2 tests. There were statistically significant differences for the proprioceptive input. CONCLUSIONS: Brief movements are needed to maintain the quiet stance. The distinctive feature of these movements is a fundamental frequency at 1.4 Hz and a harmonic frequency at 8.4 Hz. Proprioceptive input is essential for balance.

Respiratory syncytial virus persistence in macrophages downregulates intercellular adhesion molecule-1 expression and reduces adhesion of non-typeable Haemophilus influenzae.

BACKGROUND: Persistence of respiratory syncytial virus (RSV) has been associated with episodes of chronic obstructive pulmonary disease (COPD); furthermore, co-infection of RSV with non-typeable Haemophilus influenzae (NTHi) is increasingly recognized as a cause of exacerbations of COPD. OBJECTIVE: To study whether RSV persistence in a macrophage (Mφ)-like cell line alters NTHi uptake (adhesion and ingestion). METHODS: A murine Mφ-like cell line persistently infected with RSV (MφP) was used. The effects of RSV persistence on NTHi uptake by MφP and mock-infected Mφ (MφN) were determined by flow-cytometric assays with NTHi labelled with either ethidium bromide or FITC. Expression of intercellular adhesion molecule-1 (ICAM-1), a ligand for NTHi, was determined by measuring mRNA through real-time PCR and protein by Western blot assays. RESULTS: RSV persistence reduced both the capacity of Mφ to take up bacteria and the expression of ICAM-1 mRNA and protein. Furthermore, when ICAM-1 was blocked with anti-ICAM-1 antibody, the adhesion capacity of NTHi was significantly reduced for MφN, whereas for MφP the effect was less evident, implying that ICAM-1 participates in NTHi adhesion to Mφ. CONCLUSION: RSV persistence in murine Mφ diminishes their capacity to adhere and ingest NTHi through downregulation of ICAM-1 expression at the transcriptional level.