Adansonia digitata L. fruit shell extract alleviates lead-induced neurotoxicity in mice via modulation of oxidative stress and a possible chelating activity.

BACKGROUND: Lead is a ubiquitous environmental heavy metal known to induce neurotoxicity. It has been postulated that substance with high antioxidant capacity could alleviate lead-induced neurotoxicity. Adansonia digitata fruit shell extract (ADFS) has been reported to have high phenolic contents and exerts antioxidant activity. This study investigated the effects of Adansonia digitata fruit shell extract on lead-induced neurotoxicity in mice. METHODS: Male balb/c mice (n = 7) were administered with Pb-acetate (50 mg/kg) 30 mins before ADFS (250 mg/kg and 500 mg/kg) or succimer (50 mg/kg) per orally for 28 days. Motor activities were evaluated on days 29 and 30 through horizontal bar and open field tests respectively. Further, spectrophotometry, atomic absorption spectrophotometry and haematoxylin and eosin staining were carried-out to determine the expression of oxidative stress biomarkers, level of lead concentration in the brain and histology of the cerebellum respectively. RESULTS: Lead acetate exposure significantly (p < 0.05) induced motor deficits in horizontal bar test and open field test, caused oxidative stress, high concentration of lead in the brain as well as histological aberration in the cerebellum. ADFS significantly (p < 0.05) reversed the motor deficits evident by increased muscle strength and number of lines crossed. Further, ADFS significantly reversed oxidative stress evident by increased levels of SOD, CAT and GSH and decreased level of MDA. There was also significant (p < 0.05) decrease in brain lead concentration as well as reduced cerebellar cells death. CONCLUSION: Findings suggest that ADFS attenuated motor deficits via inhibition of oxidative stress and chelating activity which is comparable to succimer. Hence, ADFS should be explored for possible development of chelating agent against lead and other heavy metals toxicity.

Sonographic assessment of the portal vein diameter in apparently healthy adults in a Northern Nigerian population.

BACKGROUND: Sonography is a valuable tool in the assessment of porto-systemic pathologies. This study aimed at determining the mean portal vein diameter based on age, gender and anthropometric variables. METHODS: A cross sectional study conducted among 201 apparently healthy adults in Bauchi Metropolis. Participants were recruited from the school of nursing AbubakarTafawa Balewa Teaching Hospital (ATBUTH), Bauchi. Ultrasound machine ALOKA SSD-1000, (IP-1233EV, SN-57324, Japan) with curvilinear transducer with frequency of 3-5MHz was used for a period of four months, (December 2015 to April 2016). Participants' heights were measured while standing against a meter rule with the head in Frankfurts' position and weight measured using a weighing scale. Data analysis was done using SPSS version 22.0. Descriptive statistics (mean, standard deviation), and Pearson's Correlation were used. RESULTS: The mean portal vein diameter was 9.60 ± 1.41mm for both sexes. The mean value for males was 9.71 ± 1.42mm, and 9.35±1.46mm among females. There was a positive correlation correlation between the PV diameter and Body Mass Index (P ≤ 0.01). CONCLUSION: This study found the mean values of PV diameter in apparently healthy adults in our environment to be 9.60 ± 1.41mm and that PV diameter positively correlates with anthropometric variables.