RESUMEN
Heat shock proteins are induced in a wide range of abiotic and biotic stresses. They are well known for cellular chaperone activities and play an important role in protecting plants through regulation of homeostasis and survival. A comprehensive characterization and comparative analysis of the Hsp70 family members within the closely related plant species helps in better interpretation of these proteins' contribution to cell function and response to specific environmental stresses. Therefore, it is of interest to glean insights from the protein sequence analysis of PgHsc 70 and OsHsp70 genes. Thus, we document data from the sequence and structure analysis of PgHsc 70 and OsHsp 70 gene a.
RESUMEN
Phytophagous insect incidence is a serious threat for reduction of crop productivity globally. There is an estimation of one fourth of crop is being destroyed by insects annually. Indeed, the development of insect-resistant crops is a great milestone in agriculture to increase crop yield and reduce pesticide dependency. Genetic engineering facilitates development of insect resistant crops by expressing bacterial δ-endotoxins and vegetative insecticidal proteins and other plant genes like lectins, protease inhibitors, etc. In addition, RNA interference and genome editing through CRISPR Cas9 also provides new solutions for the development of insect-resistant crops. The resultant genetically modified crops showed resistance against lepidopteran, dipteran, homopteran and coleopteran insects. The insect-resistant crops have made a significant economic impact worldwide in terms of higher yield and low pesticide usage. In this review, we focus on different strategies for developing transgenics against insect pest control by expressing different insecticidal proteins in crops.
Asunto(s)
Toxinas de Bacillus thuringiensis/genética , Productos Agrícolas/genética , Ingeniería Genética/tendencias , Plantas Modificadas Genéticamente/genética , Animales , Bacillus thuringiensis/genética , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/parasitología , Edición Génica , Control de Insectos/tendencias , Insectos/patogenicidad , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Plantas Modificadas Genéticamente/parasitologíaRESUMEN
High-yielding Indian cotton varieties are not amenable for regeneration and transformation because they are recalcitrant in nature. In this work, we have developed Narasimha (NA1325) cotton variety by introducing three Cry genes driven by three different promoters conferring insect resistance. The meristematic region of embryo axis explants were infected and co-cultivated with Agrobacterium tumefacience (LBA4404) harbouring pMDC100 vector with three Cry gene cassettes (alpha-globulin : Cry2Ab, DECaMV35s : Cry1F and nodulin : Cry1Ac) with Npt II as a selectable marker gene. Out of 1010 embryo axes explants infected, 121 (T0) regenerated under two rounds of kanamycin selectionmedium.About 2551T1 seedswere collected from111T0 plants and these seeds screened again with kanamycin at seedling stage. The transgenic plants were characterized by PCR, real time quantitative PCR, lateral flow strip protein assay and insect bioassay. Out of 145 kanamycin resistant plants (T1), twelve showed amplification of all four transgenes: Npt II, Cry2Ab, Cry1F and Cry1Ac through PCR with expected amplicons as 395, 870, 840 and 618 bp, respectively. Further, lateral flow strip test revealed Cry1F and Cry1Ac proteins accumulated in 12 plants, whereas Cry2Ab protein was detected in eight only. The transcripts of all three Cry genes were accumulated significantly higher in transgenic plants at T2 generation. The transgenic lines showed effective resistance againstHelicoverpa armigera and Spodoptera litura larvae. The T2 line L-3 exhibited highest percentage of insect mortality, in which transcripts of all cry genes were accumulated higher than other plants. The transgenic cotton plants carrying triple Cry genes could be an excellent germplasmresource for the breeders for introgressions.
Asunto(s)
Agrobacterium tumefaciens/metabolismo , Proteínas Bacterianas/genética , Gossypium/genética , Gossypium/metabolismo , Larva/virología , Nucleopoliedrovirus/patogenicidad , Plantas Modificadas Genéticamente/genética , Animales , Proteínas de Insectos , Receptores de Superficie Celular , Transformación Genética , TransgenesRESUMEN
The prevailing abiotic stresses, especially heat stress is of great significance on the growth of plants, yield and distribution. In the conditions of heat stress, plants modulate protein processes leading to development of heat tolerance. Of such proteins, the molecular chaperone functions of HSP70/HSC70 proteins are important where their enhanced expression positively correlates with the acquisition of heat tolerance. The key players in the regulation of such tailored protein responses of plants to heat stress are the phytohormones. In the present study, phytohormone mediated interaction of Pennisetum glaucum HSC70 (PgHSC70) protein was performed through docking studies involving sequence analysis, 3D modeling and model evaluation. In silico analysis has shown better interaction and good binding energy of PgHSC70 with the phytohormone brassinolide. Furthermore, the predicted structural information can be helpful for future studies on role of interaction between HSC70 and brassinolide in heat tolerance.