Relationships between proteasomes and viral gene products.

The interrelationships between proteasomes and viral gene products are very complex. 20S proteasomes associate with a number of viral mRNAs which are cleaved by proteasome's associated endonuclease activity. In addition proteasome's endopeptidase activities are involved in the presentation of viral antigens. Viral proteins of different origin associate with the 20S and 26S complexes and interfere with their enzymatic activities. A major part of this review deals with the interactions between 20S proteasomes and the gene products of the human immunodeficiency virus (HIV) which has been studied in detail by our group.

Prospective evaluation of history and physical examination: variables to determine radiography in acute ankle injuries.

OBJECTIVE: This study investigated history and physical findings among 74 patients with acute ankle injuries in order to determine factors significantly associated with fractures, excluding avulsion fragments < 3 mm in size, and syndesmosis injuries and to determine factors that necessitate radiography. DESIGN: This was a prospective study performed during a 12-month period. After recording history and physical examination data, a gestalt prediction of a positive or negative radiographic result was made before the patient underwent ankle radiography. Analysis then determined factors important for radiography. PATIENTS AND SETTING: Patients who presented to a sports medicine center with an acute ankle injury were enrolled in the study after meeting the enrollment criteria. MAIN OUTCOME MEASURES: Before analysis, predictions for injury were based on accepted indicators. Outcome measures, factors that would indicate the need for radiography, were formulated after data collection and statistical analysis. RESULTS: Radiographic findings showed nine fractures and three widened syndesmoses as well as 15 minor ligamentous avulsions. Statistical analysis showed significant association (p < 0.05) of fracture with previous ankle fracture, syndesmosis pain with external rotation stress testing, and pain along the middle third of the distal fibula, from anterior to posterior. Syndesmosis injuries had a significant association with pain during external rotation stress testing. CONCLUSIONS: Although additional investigation with larger patient numbers would be beneficial, this study highlights the importance of history of previous fracture, pain on the distal mid-fibula or mid-tibia, and pain with external rotation. Furthermore, if these three variables are prospectively applied as criteria for radiography, a 55% reduction in radiography would result with 100% sensitivity. Finally, experienced sports medicine physicians had a 100% sensitivity, 68% specificity, 100% negative predictive value, and 39% positive predictive value for prediction of clinically significant fractures or syndesmosis injuries.