The stress of the SARS-CoV-2 virus pandemic and pro-health behaviors among medical personnel - preliminary report. / Stres okresu pandemii wirusa SARS-CoV-2 a zachowania prozdrowotne wsród personelu medycznego - doniesienie wstepne.

OBJECTIVES: The aim of the study was to evaluate pro-health behaviors as protective measures against symptoms of anxiety and depression in a group of health care workers during the first wave of the SARS-CoV-2 virus pandemic. METHODS: The group of 114 people participated in the study, including 46 medical doctors aged 41.10 ± 11.89 and 68 nurses aged 48.16 ± 8.54 years.The following scales were used for the research: the Health Behavior Inventory (HBI), the Beck Depression Inventory (BDI) and the State-Trait Anxiety Inventory (STAI). RESULTS: Taking into consideration health behaviors, an average score of 79.61 ± 13.08 points in HBI was obtained. In the BDI questionnaire, the respondents obtained an average of 3.7 ± 4.65 points. In the STAI questionnaire, in the part related to state anxiety, the mean result in the study group was 38.08 ± 9.46 points, and for trait anxiety 38.35 ± 8.44 points. Taking into account the components of HBI, only the results obtained in the subscales: positive mental attitude (PMA) and pro-health activities (PhA) correlated negatively with the results obtained in the STAI and BDI scales. Moreover, the pro-health effect of PMA on the symptoms of anxiety and depression was observed. CONCLUSIONS: No significant intensification of anxiety and depression symptoms was observed among medical personnel during the first wave of the pandemic. Health-promoting behaviors, and especially positive mental attitudes, may play a protective role in relation to the symptoms of anxiety and depression in a stressful situations.

SARS-CoV-2 Antibody Screening in Healthcare Workers in Non-Infectious Hospitals in Two Different Regions of Southern Poland (Upper Silesia and Opole Voivodeships): A Prospective Cohort Study.

(1) Background: Detection of asymptomatic or subclinical human coronavirus SARS-CoV-2 infection in healthcare workers (HCWs) is crucial for understanding the overall prevalence of the new coronavirus and its infection potential in public (non-infectious) healthcare units with emergency wards. (2) Methods: We evaluated the host serologic responses, measured with semi-quantitative ELISA tests (IgA, IgG, IgM abs) in sera of 90 individuals in Hospital no. 4 in Bytom, 84 HCWs in the University Hospital in Opole and 25 in a Miasteczko Slaskie local surgery. All volunteers had negative RT-PCR test results or had not had the RT-PCR test performed within 30 days before sampling. The ELISA test was made at two different time points (July/August 2020) with a 2-weeks gap between blood collections to avoid the "serological window" period. (3) Results: The IgG seropositivity of asymptomatic HCWs varied between 1.2% to 10% (Opole vs. Bytom, p < 0.05; all without any symptoms). IgA seropositivity in HCWs was 8.8% in Opole and 7.14% in Bytom. IgM positive levels in HCWs in Opole and Bytom was 1.11% vs. 2.38%, respectively. Individuals with IgA and IgM seropositivity results were observed only in Opole (1.19%). More studies are needed to determine whether these results are generalizable to other populations and geographic as well as socio-demographic locations. (4) Conclusions: 100% of IgG(+) volunteers were free from any symptoms of infection in the 30 days before first or second blood collection and they had no awareness of SARS-CoV-2 infection. Asymptomatic HCWs could spread SARS-CoV-2 infection to other employees and patients. Only regular HCWs RT-PCR testing can reduce the risk of SARS-CoV-2 spreading in a hospital environment. The benefit of combining the detection of specific IgA with that of combined specific IgM/IgG is still uncertain.

[The quality of platelet concentrates in dependence on storage time before pathogen inactivation]. / Jakosc koncentratów plytek krwi w zaleznosci od czasu ich przechowywania przed wykonaniem procedury inaktywacji patogenów.

UNLABELLED: Pathogen inactivation procedure performed just before distribution of platelet concentrates (PCs) may decrease costs caused by loss of these components due to relatively short expiry date. THE AIM OF STUDY: To evaluate the quality of PCs pathogen inactivated on the first or the fifth day of storage. MATERIAL AND METHODS: PCs preparated from buffy-coats were suspended in platelet additive solution (Intersol, Baxter Healthcare Corporation, Belgium). The photochemical pathogen inactivation was performed on the 1st or the 5th day of storage using amotosalen and UVA (Cerus, Europe BV). PCs were stored for 7 days. RESULTS: There were observed increased expression of CD62 and CD63, elevated activity of LDH and lower concentration of glucose in PCs pathogen inactivated on day 1 compare to the control group. PCs pathogen inactivated on day 5 showed decreased expression of CD62 and CD63 compare to the control group. There were no significant differences in platelet number, pH, lactate concentration, hypotonic shock response and release of platelet derived microparticles in both groups of pathogen inactivated PCs. CONCLUSIONS: Time of storage of PCs before pathogen inactivation has no significant impact on PCs quality. Pathogen inactivation procedure performed just after having received request for PCs is more cost effective than the routine pathogen inactivation in all PCs before storage.