RESUMEN
Event cameras triggered a paradigm shift in the computer vision community delineated by their asynchronous nature, low latency, and high dynamic range. Calibration of event cameras is always essential to account for the sensor intrinsic parameters and for 3D perception. However, conventional image-based calibration techniques are not applicable due to the asynchronous, binary output of the sensor. The current standard for calibrating event cameras relies on either blinking patterns or event-based image reconstruction algorithms. These approaches are difficult to deploy in factory settings and are affected by noise and artifacts degrading the calibration performance. To bridge these limitations, we present E-Calib, a novel, fast, robust, and accurate calibration toolbox for event cameras utilizing the asymmetric circle grid, for its robustness to out-of-focus scenes. E-Calib introduces an efficient reweighted least squares (eRWLS) method for feature extraction of the calibration pattern circles with sub-pixel accuracy and robustness to noise. In addition, a modified hierarchical clustering algorithm is devised to detect the calibration grid apart from the background clutter. The proposed method is tested in a variety of rigorous experiments for different event camera models, on circle grids with different geometric properties, on varying calibration trajectories and speeds, and under challenging illumination conditions. The results show that our approach outperforms the state-of-the-art in detection success rate, reprojection error, and pose estimation accuracy.
RESUMEN
The computer vision algorithms used currently in advanced driver assistance systems rely on image-based RGB cameras, leading to a critical bandwidth-latency trade-off for delivering safe driving experiences. To address this, event cameras have emerged as alternative vision sensors. Event cameras measure the changes in intensity asynchronously, offering high temporal resolution and sparsity, markedly reducing bandwidth and latency requirements1. Despite these advantages, event-camera-based algorithms are either highly efficient but lag behind image-based ones in terms of accuracy or sacrifice the sparsity and efficiency of events to achieve comparable results. To overcome this, here we propose a hybrid event- and frame-based object detector that preserves the advantages of each modality and thus does not suffer from this trade-off. Our method exploits the high temporal resolution and sparsity of events and the rich but low temporal resolution information in standard images to generate efficient, high-rate object detections, reducing perceptual and computational latency. We show that the use of a 20 frames per second (fps) RGB camera plus an event camera can achieve the same latency as a 5,000-fps camera with the bandwidth of a 45-fps camera without compromising accuracy. Our approach paves the way for efficient and robust perception in edge-case scenarios by uncovering the potential of event cameras2.
RESUMEN
Natural microbial communities are phylogenetically and metabolically diverse. In addition to underexplored organismal groups1, this diversity encompasses a rich discovery potential for ecologically and biotechnologically relevant enzymes and biochemical compounds2,3. However, studying this diversity to identify genomic pathways for the synthesis of such compounds4 and assigning them to their respective hosts remains challenging. The biosynthetic potential of microorganisms in the open ocean remains largely uncharted owing to limitations in the analysis of genome-resolved data at the global scale. Here we investigated the diversity and novelty of biosynthetic gene clusters in the ocean by integrating around 10,000 microbial genomes from cultivated and single cells with more than 25,000 newly reconstructed draft genomes from more than 1,000 seawater samples. These efforts revealed approximately 40,000 putative mostly new biosynthetic gene clusters, several of which were found in previously unsuspected phylogenetic groups. Among these groups, we identified a lineage rich in biosynthetic gene clusters ('Candidatus Eudoremicrobiaceae') that belongs to an uncultivated bacterial phylum and includes some of the most biosynthetically diverse microorganisms in this environment. From these, we characterized the phospeptin and pythonamide pathways, revealing cases of unusual bioactive compound structure and enzymology, respectively. Together, this research demonstrates how microbiomics-driven strategies can enable the investigation of previously undescribed enzymes and natural products in underexplored microbial groups and environments.
Asunto(s)
Vías Biosintéticas , Microbiota , Océanos y Mares , Bacterias/clasificación , Bacterias/genética , Vías Biosintéticas/genética , Genómica , Microbiota/genética , Familia de Multigenes/genética , FilogeniaRESUMEN
Antibiotic resistance spread via plasmids is a serious threat to successfully fight infections and makes understanding plasmid transfer in nature crucial to prevent the rise of antibiotic resistance. Studies addressing the dynamics of plasmid conjugation have yet neglected one omnipresent factor: prophages (viruses integrated into bacterial genomes), whose activation can kill host and surrounding bacterial cells. To investigate the impact of prophages on conjugation, we combined experiments and mathematical modelling. Using Escherichia coli, prophage λ and the multidrug-resistant plasmid RP4 we find that prophages can substantially limit the spread of conjugative plasmids. This inhibitory effect was strongly dependent on environmental conditions and bacterial genetic background. Our empirically parameterized model reproduced experimental dynamics of cells acquiring either the prophage or the plasmid well but could only reproduce the number of cells acquiring both elements by assuming complex interactions between conjugative plasmids and prophages in sequential infections. Varying phage and plasmid infection parameters over empirically realistic ranges revealed that plasmids can overcome the negative impact of prophages through high conjugation rates. Overall, the presence of prophages introduces an additional death rate for plasmid carriers, the magnitude of which is determined in non-trivial ways by the environment, the phage and the plasmid. This article is part of the theme issue 'The secret lives of microbial mobile genetic elements'.