RESUMEN
Tidal breathing is associated with a 30% change of the surfactant-covered alveolar surface occurring about 16 times per minute. To model this highly dynamic process, erucic acid monolayers at the air-water interface were compressed fast. Brewster angle microscopy imaged the fractal liquid-condensed (LC) domains and quantified the surface flow in size, direction, and duration. Radial branch distribution of the domains has a minimum in the flow direction, as was shown with directionality histograms. The fast Fourier transform of the domains shows a preferential growth perpendicular to the flow direction. Additionally, at the beginning of the flow, the downstream side of the domain grows faster than the upstream side. Surface flows act on the mm to cm scale, cause an anisotropic flow in the liquid expanded phase surrounding the LC domain, and affect the overall domain shape. On the µm-scale, the dendritic or seaweed domains' branches were only slightly disturbed. These results may help to understand pulmonary surfactant layers.
RESUMEN
The lateral movement in lipid membranes depends on their diffusion constant within the membrane. However, when the flux of the subphase is high, the convective flow beneath the membrane also influences lipid movement. Lipid monolayers of an unsaturated fatty acid at the water-air interface serve as model membranes. The formation of domains in the liquid/condensed coexistence region is investigated. The dimension of the domains is fractal, and they grow with a constant growth velocity. Increasing the compression speed of the monolayer induces a transition from seaweed growth to dendritic growth. Seaweed domains have broad tips and wide and variable side branch spacing. In contrast, dendritic domains have a higher fractal dimension, narrower tips, and small, well-defined side branch spacing. Additionally, the growth velocity is markedly larger for dendritic than seaweed growth. The domains' growth velocity increases and the tip radius decreases with increasing supersaturation in the liquid/condensed coexistence region. Implications for membranes are discussed.
RESUMEN
Lipid oxidation does strongly influence the self-organization of plasma membranes; the detailed influence is not yet clear. In this work, phospholipid monolayers at the air/water interface were used as model membranes. Oxidation was induced by the reactive oxygen species formed in a H2O2-enriched solution. The reaction was found to be diffusion-limited; the concentration of the reactive oxygen species was about 50 nM. Isotherms were recorded for different phosphatidylcholines with saturated and unsaturated acyl chains. For unsaturated lipids, the isotherms showed a constant relative molecular area increase after oxidization, independent of the molecular area and dependent on the degree of peroxidation. Similarly, the compressibility modulus was unchanged, but shifted to larger molecular areas. The correlation between peroxidation and changes of the interaction forces between the lipid molecules is discussed.