Diagnostic and prognostic role of cancer stem cell biomarkers in oral squamous cell carcinoma; A Systematic Review.

Objectives: To evaluate the role of cancer stem cell biomarkers in diagnosis and prognosis of OSCC patients. METHODS: The search strategy was entered into PubMed NLM, EBSCO CINAHL, EBSCO Dentistry & Oral Sciences Source, Wiley Cochrane Library, and Scopus. The full text eligible studies (n=7) were assessed for their quality using the JBI Critical Appraisal Checklist to evaluates the methodological quality of the studies based on possibility of bias in its design, conduct, and analysis. Selected studies were further analysed based on different parameters such as publication year, sample size, and outcomes. RESULTS: A total of 432 studies were identified through the search strategy. A total of 306 records were removed before screening either because of duplication or marked ineligible by the automation tools. The screened records were 126 out of which 104 were removed as they were not conducted on OSCC. Twenty-two reports were sought for retrieval, however, we could not find the full text of 3 studies and12 studies were excluded because the biomarkers were not associated with cancer stem cells. The most common cancer stem cell biomarkers associated with OSCC were MCT1,VEGF-A, GD15, HIF1 α, Ki67, Hsp 70, Cyclin D1, and CD44. CONCLUSIONS: Various stem cell biomarkers have been found to have diagnostic and prognostic role in oral squamous cell carcinoma such as Cyclin D1, VEGF-A, GD15, and CD44. They can be used to predict the overall survival rate, local progression-free survival rate, and distant metastasis-free survival rate in Head and Neck cancer patients.

Evaluation of Social Media Usage by Dental Practitioners of Pakistan for Professional Purposes - A Cross-Sectional Study.

Background: In the past couple of years, the use of social media has been on a tremendous rise with greater attention in the field of healthcare, including dentistry. The use of social media for professional purposes is emphasized as unprofessional use can be detrimental. This study aims to evaluate the use of social media by dentists for professional purposes. Materials and Methods: This cross-sectional study was carried out between December 2021 and March 2022. An online questionnaire was formulated using Google Forms and distributed to the dental professionals through various social media platforms such as Twitter©, Facebook©, Instagram©, Snapchat©, WhatsApp©, and Email©. The questionnaire consisted of two parts: demographic characteristics and questions about the use of social media for professional purposes. For data analysis, SPSS version 25.0 was used, where a multiple linear regression test was used to compare the responses of the participants with their demographic characteristics. A p-value of ≤0.05 was considered to be statistically significant. Results: A total of 404 dentists participated in this study with a response rate of 89.7%. The majority of the 276 (68.3%) dentists believed to have a professional account to use social media for professional purposes. Most of the 280 (69.3%) dentists agreed social media improved knowledge and communication skills with the patients. About 248 (61.4%) dentists considered the use of social media for general purposes during clinical hours led to wastage of time. About 160 (39.6%) dentists considered connecting with patients on social media to be inappropriate. Conclusion: The use of social media is on the rise at a tremendous rate in different fields of work, including dentistry. Social media is a great tool for dentists to help expand and grow their clinical practice. However, the professional use and ethics whilst using social media should be considered by the dentists.

Artificial intelligence in dentistry, orthodontics and Orthognathic surgery: A literature review.

Artificial intelligence is the ability of machines to work like humans. The concept initially began with the advent of mathematical models which gave calculated outputs based on inputs fed into the system. This was later modified with the introduction of various algorithms which can either give output based on overall data analysis or by selection of information within previous data. It is steadily becoming a favoured mode of treatment due to its efficiency and ability to manage complex conditions in all specialities. In dentistry, artificial intelligence has also popularised over the past few decades. They have been found useful for diagnosis in restorative dentistry, oral pathology and oral surgery. In orthodontics, they have been utilised for diagnosis, assessment of treatment needs, cephalometrics, treatment planning and orthognathic surgeries etc. The current literature review was planned to highlight the uses of artificial intelligence in dentistry, specifically in orthodontics and orthognathic surgery.

Management of difficult airway in a pregnant patient with severely reduced mouth opening.

Pregnancy is associated with a wide variety of oral and dental changes ranging from gingivitis to odontogenic infections. If left untreated, severe dental abscess can progress to Ludwig's angina, which is a potentially lethal cellulitis that rapidly extends to the neck region and may lead to life-threatening upper airway obstruction. We report the case of a pregnant woman who presented with intense throbbing pain, trismus and severely reduced mouth opening due to dental abscess for the last one week. She required incision and drainage of abscess with extraction of third molar under general anaesthesia. There are some significant challenges to anaesthesiologist like the risk of aspiration and failed intubation in patients with pregnancy and anticipated difficult airway. With counselling and proper preparation, we were able to manage this case with awake intubation. The potential merits of securing airway in conscious state with necessary steps in preparation are particularly discussed with emphasis of creating awareness among local healthcare practitioners.

Stress and anxiety among dental practitioners during the COVID-19 pandemic: A cross-sectional survey.

BACKGROUND: Dental professionals are at great risk of contracting coronavirus disease 2019 (COVID-19). OBJECTIVES: The objectives of this study were to determine the levels of stress and anxiety among dental professionals, and to determine which dental procedures cause the greatest amount of stress and anxiety during the COVID-19 pandemic. MATERIAL AND METHODS: This cross-sectional survey was conducted by requesting voluntary participation of dental healthcare workers through the authors' own e-form, which consisted of our self-developed questionnaire, the Perceived Stress Scale (PSS) and the Generalized Anxiety Disorder-7 scale (GAD-7). The simple and multiple linear regression analyses were used to assess the effect of dental procedures and other factors associated with stress and anxiety among the participants. A p-value ≤0.05 was considered statistically significant. RESULTS: This survey included 85 participants (32 males, 53 females) with a mean age of 31.6 ±6.0 years. Significant associations were found between severe stress for scaling (p < 0.001; p < 0.001), complex fillings (p < 0.001; p < 0.001), root canal treatment (RCT) (p = 0.001; p = 0.007), crown and bridge work (p < 0.001; p < 0.001), denture work (p = 0.034; p = 0.001), third molar extractions (p < 0.001; p < 0.001), surgical procedures (p < 0.001; p = 0.001), and implant placement (p = 0.001; p = 0.022) and the PSS and GAD-7 scores, respectively. CONCLUSIONS: Dental healthcare workers exhibit severe stress and anxiety associated with elective dental procedures. Dental emergencies should take precedence and elective dental treatment should be carried out with utmost caution, ensuring all protective measures. Psychological support for dental healthcare professionals should be made accessible.

Ameloblastoma: a surgeon's dilemma.

PURPOSE: To investigate whether there were any significant differences in the mode of presentation, treatment, and outcome of patients presenting with a primary diagnosis of ameloblastoma in Glasgow, Scotland and San Francisco, CA. MATERIALS AND METHODS: All cases of ameloblastoma seen in both institutions between January 1, 1980 and December 31, 1999 were included in this study. Mode of presentation, radiographic appearance, histologic appearance, treatment, and follow-up were recorded. RESULTS: There were no significant differences in the clinical features on presentation (swelling, followed by pain, and altered sensation), the radiographic appearance (unilocular approximately 30% and multilocular 70%), or management with either local treatment (enucleation and/or curettage in just over 50% of cases) or radical treatment (a form of resection in under 50%) in the 50 cases included in this study. Primary care by conservative treatment led to a recurrence in approximately 80% of cases and this included cases of unicystic ameloblastoma. CONCLUSION: The mode of presentation, diagnosis, and management of the ameloblastoma was remarkably similar in Glasgow and San Francisco. The recurrence rate following local enucleation and curettage was unacceptably high, and this included the cases of unicystic ameloblastoma, which should be treated more aggressively than has been recommended in the past.