Mechanical performance of a conventional resin composite and its bulk-fill restorative counterpart after long-term accelerated aging.

BACKGROUND: The long-term mechanical properties and stability of various resin composites in areas under stress are questionable. OBJECTIVES: The aim of the study was to determine the effects of long-term hydrothermal aging on the mechanical properties of a microhybrid conventional resin composite and its bulk-fill counterpart. MATERIAL AND METHODS: We used a conventional and high-viscosity restorative bulk-fill resin-based composites (RBCs) from one company. Bar-shaped specimens of each type of resin composite were fabricated using steel molds and divided into 2 groups. The specimens were stored at 37°C for 24 h, and half of the specimens in each group were subjected to the 3-point bending flexural test and microhardness measurement. The remaining specimens were aged for 10,000 thermal cycles between 5°C and 55°C, and then subjected to flexural testing and microhardness measurement. A Vickers microhardness tester was used to estimate the surface microhardness of the specimens. Data was analyzed using an independent t test and the Mann-Whitney U test. The statistical significance level was set at p ≤ 0.05. Scanning electron microscopy (SEM) was used to investigate the surface of each material. RESULTS: The bulk-fill RBC showed similar flexural strength and modulus to its conventional counterpart before aging. The flexural strength of both resin composites was significantly decreased after thermocycling (p < 0.001). Hydrothermal aging had no significant effects on the flexural modulus (p = 0.84). There was a significant decrease in the surface microhardness of the bulk-fill RBC. Scanning electron microscopy photomicrographs showed several pits as a result of the exfoliation of the filler particles on the surface of the bulk-fill RBC after aging. CONCLUSIONS: The flexural strength of both resin composites decreased significantly after aging. The flexural properties, surface changes and microhardness of the bulk-fill type were additionally affected by the aging process.

Ion release, biocompatibility, and bioactivity of resin-modified calcium hydroxide cavity liners.

BACKGROUND: The placement of liners near the pulp area is essential for therapeutic effects and maintaining pulp health while stimulating the formation of tertiary dentin. This in vitro study aimed to evaluate the calcium release, pH, biocompatibility, solubility, and bioactivity of three resin-modified calcium hydroxide cavity liners. METHODS: The disc specimens of each cavity liner were prepared using polyethylene molds of 7 mm in diameter and 2 mm in height (n = 10). Three light-cure liners evaluated include Ultra-Blend Plus (UB), Base-it (BI), and Master Dent (MD). The samples were then immersed in flasks containing 10 mL of distilled water. Calcium ion release, pH, and solubility were evaluated in two weeks of incubation. The cytotoxicity of extracts adjacent to the specimens was evaluated by MTT assay using NIH/3T3 cells after 1, 3, and 7 days of incubation. The ability to induce the nucleation of calcium phosphates (CaPs) after 28-day immersion in a simulated body fluid was investigated by SEM-EDX analysis. Statistical analysis was performed using ANOVA, Kruskal-Wallis, and repeated measures tests at the significant level of 0.05. RESULTS: There was a significant difference in the release of calcium ions among the three liners investigated on days 1, 7, and 14 (p < 0.05). UB liners exhibited a significantly higher amount of calcium release than the other two liners, followed by BI, and MD. On day 1, there was no significant difference in the average pH among the three liners. However, after day 7, the MD liner showed a significant decrease in pH compared to the other two liners. BI liner demonstrated the highest level of biocompatibility, followed by the MD and UB liners. UB showed a high calcium release, solubility with no alkalizing activity, and the formation of more mature Ca-rich apatite deposits than the other two liners. CONCLUSION: Based on the results of this study, the cavity liner material's performance is material dependent. It can impact ion release, biocompatibility, and bioactivity which are important factors to consider in clinical practice. Further studies are needed to investigate the long-term effects of different liner materials on oral tissues.

Comparison of mechanical properties, surface roughness, and color stability of 3D-printed and conventional heat-polymerizing denture base materials.

STATEMENT OF PROBLEM: Studies on the mechanical, optical, and surface properties of 3-dimensionally (3D) printed denture base materials are scarce, and those available have reported conflicting results. PURPOSE: The purpose of this in vitro study was to compare the mechanical properties, surface roughness, and color stability of 3D-printed and conventional heat-polymerizing denture base materials. MATERIAL AND METHODS: A total of 34 rectangular specimens (64×10×3.3 mm) were fabricated from each of the conventional (SR Triplex Hot; Ivoclar AG) and 3D-printed (Denta base; Asiga) denture base materials. All specimens underwent coffee thermocycling for 5000 cycles, and half in each group (n=17) were evaluated in terms of color parameters, color change (ΔE00), and surface roughness (Ra) before and after coffee thermocycling. The specimens then underwent a 3-point bend test. The remaining specimens in each group (n=17) underwent impact strength and Vickers hardness testing. Data were analyzed by the paired samples, independent samples, and Wilcoxon signed rank tests (α=.05). RESULTS: The color change caused by coffee thermocycling in the 3D-printed group was higher than that in the conventional group (P<.001). Surface roughness significantly increased in both groups after coffee thermocycling (P<.001). The conventional group had higher surface roughness before coffee thermocycling, while the 3D-printed group had higher surface roughness after coffee thermocycling (P<.001). The flexural strength, flexural modulus, and surface hardness in the conventional group were significantly higher than those in the 3D-printed group (P<.001). However, the impact strength of the conventional group was lower than that of the 3D-printed group (P<.001). CONCLUSIONS: The 3D-printed denture base material showed higher impact strength and surface roughness than the conventional heat-polymerizing acrylic resin. However, flexural strength and modulus, surface hardness, and color stability were lower in the 3D-printed group.

Drug release kinetics and biological properties of a novel local drug carrier system.

BACKGROUND: The purpose of this in vitro study was to investigate drug release kinetics and cytotoxicity of a novel drug delivery system for treatment of periodontitis. MATERIALS AND METHODS: This in vitro study addresses the fabrication of a polycaprolactone/alginic acid-based polymeric film loaded with metronidazole, as a basic drug in the treatment of periodontal diseases. Films were prepared by solvent casting technique. Four formulations with different percentages of drug by weight (3%, 5%, 9%, and 13%) were prepared. Drug release kinetics were investigated using ultraviolet-visible spectroscopy during (one week). Data were analyzed using repeated measures ANOVA. Cytotoxicity of drug-loaded system extracts was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 24-h incubation. The results were evaluated according to ISO standard 10993-5 and assessed using ANOVA and Tukey's tests at a significance level of P < 0.05. RESULTS: All polymeric films showed a burst drug release followed by a gradual release. Drug release data were fitted well with the first-order kinetic model in all drug-containing formulations indicating that drug release is a fraction of remaining drug in the matrix. Drug release is mainly driven by diffusion of medium into the composite matrix. 3%wt metronidazole-containing formulation exhibited the best MTT result. CONCLUSION: The findings of this study supported the synthesis of drug-loaded periodontal films with 3% metronidazole due to better biological properties along with the ability of acceptable drug release to eradicate anaerobic periodontal bacteria.

Micro-computed tomography in preventive and restorative dental research: A review.

PURPOSE: The use of micro-computed tomography (micro-CT) scans in biomedical and dental research is growing rapidly. This study aimed to explore the scientific literature on approaches and applications of micro-CT in restorative dentistry. MATERIALS AND METHODS: An electronic search of publications from January 2009 to March 2021 was conducted using ScienceDirect, PubMed, and Google Scholar. The search included only English-language articles. Therefore, only studies that addressed recent advances and the potential uses of micro-CT in restorative and preventive dentistry were selected. RESULTS: Micro-CT is a tool that enables 3-dimensional imaging on a small scale with very high resolution. In this method, there is no need for sample preparation or slicing. Therefore, it is possible to examine the internal structure of tissue and the internal adaptation of materials to surfaces without destroying them. Due to these advantages, micro-CT has been recommended as a standard imaging tool in dental research for many applications such as tissue engineering, endodontics, restorative dentistry, and research on the mineral density of hard tissues and bone growth. However, the high costs of micro-CT, the time necessary for scanning and reconstruction, computer expertise requirements, and the enormous volume of information are drawbacks. CONCLUSION: The potential of micro-CT as an emerging, accurate, non-destructive approach is clear, and the valuable research findings reported in the literature provide an impetus for researchers to perform future studies focusing on employing this method in dental research.

Effect of Silane Heat Treatment by Laser on the Bond Strength of a Repair Composite to Feldspathic Porcelain.

PURPOSE: Ceramic restoration fracture may occur in the oral cavity. Intraoral repair of fractured porcelain could be advantageous to both patient and dentist. The aim of this study was to evaluate the effect of heat treatment of the silane coupling agent by Er:YAG and CO2 lasers on the microshear bond strength of a repair composite to feldspathic porcelain. MATERIALS AND METHODS: Sixty ceramic blocks were prepared and randomly divided into six groups (n = 10): (i) HF + silane (HS); (ii) silane + CO2 laser (SC); (iii) CO2 laser + silane (CS); (iv) silane + Er:YAG laser (SE); (v) Er:YAG laser + silane (ES); (vi) bur + HF + silane (BuHS). An adhesive resin was applied to the prepared ceramic surfaces and light-cured. Two transparent plastic tubes were placed perpendicularly to each ceramic block. The composite resin was then placed on the treated ceramic surface and light-cured for 40 seconds. The bonded blocks were stored in distilled water at 37°C for 24 hours and subjected to 3000 thermocycles. Microshear bond strength (µSBS) tests were performed using a wire and loop method. Data were analyzed using one-way ANOVA and Duncan's multiple range tests (p ≤ 0.05). RESULTS: The comparison of the mean µSBS values showed no significant differences between the ES and HS groups (p = 0.914). On the other hand, the specimens in these groups exhibited significantly higher bond strengths than those in the other groups (p < 0.01). The mean µSBS of the BuHS group was statistically similar to that of CS and SE groups (p > 0.05). The µSBS for the SC group was significantly lower than that of the other groups (p < 0.01), with the exception of the CS group (p = 0.674). CONCLUSIONS: Treatment with Er:YAG laser prior to silane application can be as effective as HF etching. Heat treatment of silane by CO2 or Er:YAG lasers does not improve the bond strength between feldspathic porcelain and composite resin.

Comparative assessment of the crystalline structures of powder and bulk human dental enamel by X-ray diffraction analysis.

OBJECTIVES: The aim of this study was to assess and compare the crystalline structures of both powder and bulk human dental enamel by X-ray diffraction analysis (XRD). METHODS: The buccal surfaces of 60 selected noncarious third molars were divided into two groups of powdered and bulk enamel specimens. To prepare enamel powders, the enamel tissues were ground and powdered manually using a mortar and pestle. For bulk samples, the enamel slabs were sectioned using a low-speed diamond saw. The crystalline structures of samples were analyzed by XRD, and the obtained data were analyzed. The values of the lattice parameters were obtained using the equation of hexagonal crystalline structures. The crystal sizes and microstrains of crystallites were calculated using both the Scherrer and Williamson-Hall (W-H) methods. The total area under the peaks was considered as the criterion for assessing crystallinity. The data were analyzed by a parametric independent t-test and nonparametric Mann-Whitney test using SPSS for Windows at a significance level of 0.05. RESULTS: The results of the current study revealed significant differences between the crystallinity values and crystal sizes of the two study groups (p ≤ 0.001). The powdered samples showed higher crystallinity and smaller crystal sizes than those of the bulk samples. The obtained strain values of the powder samples were nearly twice those of the bulk samples. This difference was not statistically significant (p = 0.76). CONCLUSIONS: The results of this study revealed that the sample preparation method for human tooth enamel can affect the crystalline data obtained from XRD analysis.

The effect of thermocycling on the degree of conversion and mechanical properties of a microhybrid dental resin composite.

OBJECTIVE: The purpose of this study was to investigate the degree of conversion (DC) and mechanical properties of a microhybrid Filtek Z250 (3M ESPE) resin composite after aging. METHOD: The specimens were fabricated using circular molds to investigate Vickers microhardness (Vickers hardness number [VHN]) and DC, and were prepared according to ISO 4049 for flexural strength testing. The initial DC (%) of discs was recorded using attenuated total reflectance-Fourier transforming infrared spectroscopy. The initial VHN of the specimens was measured using a microhardness tester under a load of 300 g for 15 seconds and the flexural strength test was carried out with a universal testing machine (crosshead speed, 0.5 mm/min). The specimens were then subjected to thermocycling in 5°C and 55°C water baths. Properties were assessed after 1,000-10,000 cycles of thermocycling. The surfaces were evaluated using scanning electron microscopy (SEM). Data were analyzed using 1-way analysis of variance followed by the Tukey honest significant difference post hoc test. RESULTS: Statistical analysis showed that DC tended to increase up to 4,000 cycles, with no significant changes. VHN and flexural strength values significantly decreased upon thermal cycling when compared to baseline (p < 0.05). However, there was no significant difference between initial and post-thermocycling VHN results at 1,000 cycles. SEM images after aging showed deteriorative changes in the resin composite surfaces. CONCLUSIONS: The Z250 microhybrid resin composite showed reduced surface microhardness and flexural strength and increased DC after thermocycling.

Analytical methods for the measurement of polymerization kinetics and stresses of dental resin-based composites: A review.

Resin-based composites are commonly used restorative materials in dentistry. Such tooth-colored restorations can adhere to the dental tissues. One drawback is that the polymerization shrinkage and induced stresses during the curing procedure is an inherent property of resin composite materials that might impair their performance. This review focuses on the significant developments of laboratory tools in the measurement of polymerization shrinkage and stresses of dental resin-based materials during polymerization. An electronic search of publications from January 1977 to July 2016 was made using ScienceDirect, PubMed, Medline, and Google Scholar databases. The search included only English-language articles. Only studies that performed laboratory methods to evaluate the amount of the polymerization shrinkage and/or stresses of dental resin-based materials during polymerization were selected. The results indicated that various techniques have been introduced with different mechanical/physical bases. Besides, there are factors that may contribute the differences between the various methods in measuring the amount of shrinkages and stresses of resin composites. The search for an ideal and standard apparatus for measuring shrinkage stress and volumetric polymerization shrinkage of resin-based materials in dentistry is still required. Researchers and clinicians must be aware of differences between analytical methods to make proper interpretation and indications of each technique relevant to a clinical situation.