Design of bone scaffolds with calcium phosphate and its derivatives by 3D printing: A review.

Tissue engineering is a fascinating field that combines biology, engineering, and medicine to create artificial tissues and organs. It involves using living cells, biomaterials, and bioengineering techniques to develop functional tissues that can be used to replace or repair damaged or diseased organs in the human body. The process typically starts by obtaining cells from the patient or a donor. These cells are then cultured and grown in a laboratory under controlled conditions. Scaffold materials, such as biodegradable polymers or natural extracellular matrices, are used to provide support and structure for the growing cells. 3D bone scaffolds are a fascinating application within the field of tissue engineering. These scaffolds are designed to mimic the structure and properties of natural bone tissue and serve as a temporary framework for new bone growth. The main purpose of a 3D bone scaffold is to provide mechanical support to the surrounding cells and guide their growth in a specific direction. It acts as a template, encouraging the formation of new bone tissue by providing a framework for cells to attach, proliferate, and differentiate. These scaffolds are typically fabricated using biocompatible materials like ceramics, polymers, or a combination of both. The choice of material depends on factors such as strength, biodegradability, and the ability to facilitate cell adhesion and growth. Advanced techniques like 3D printing have revolutionized the fabrication process of these scaffolds. Using precise layer-by-layer deposition, it allows for the creation of complex, patient-specific geometries, mimicking the intricacies of natural bone structure. This article offers a brief overview of the latest developments in the research and development of 3D printing techniques for creating scaffolds used in bone tissue engineering.

A high-absorbance water-soluble photoinitiator nanoparticle for hydrogel 3D printing: synthesis, characterization and in vitro cytotoxicity study.

Light-based hydrogel crosslinking is a new approach in rapid and high-resolution 3D printing; however, using this method in tissue engineering is challenging due to the toxicity of photoinitiators, their solvents, and their low efficiency. Herein, a novel, water-soluble photoinitiator with high efficiency in light-based 3D printing is introduced. Low-cost photoinitator, 2,4,6-trimethylbenzoylphenyl phosphinate, is converted into nanoparticles via a microemulsion method and dispersed in water. Cell toxicity assays were performed to prove that these nanoparticles are non-toxic and can be used in biomedical applications. Finally, the nanoparticles were utilized in the high-accuracy 3D printing of hydrogels. The results of this study indicate that these particles are potent to be used in bioprinting.

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration.

BACKGROUND: Worldwide, many people suffer from knee injuries and articular cartilage damage every year, which causes pain and reduces productivity, life quality, and daily routines. Medication is currently primarily used to relieve symptoms and not to ameliorate cartilage degeneration. As the natural healing capacity of cartilage damage is limited due to a lack of vascularization, common surgical methods are used to repair cartilage tissue, but they cannot prevent massive damage followed by injury. MAIN BODY: Functional tissue engineering has recently attracted attention for the repair of cartilage damage using a combination of cells, scaffolds (constructs), biochemical factors, and biomechanical stimuli. As cyclic biomechanical loading is the key factor in maintaining the chondrocyte phenotype, many studies have evaluated the effect of biomechanical stimulation on chondrogenesis. The characteristics of hydrogels, such as their mechanical properties, water content, and cell encapsulation, make them ideal for tissue-engineered scaffolds. Induced cell signaling (biochemical and biomechanical factors) and encapsulation of cells in hydrogels as a construct are discussed for biomechanical stimulation-based tissue regeneration, and several notable studies on the effect of biomechanical stimulation on encapsulated cells within hydrogels are discussed for cartilage regeneration. CONCLUSION: Induction of biochemical and biomechanical signaling on the encapsulated cells in hydrogels are important factors for biomechanical stimulation-based cartilage regeneration.

Cell encapsulation in alginate-based microgels using droplet microfluidics; a review on gelation methods and applications.

Cell encapsulation within the microspheres using a semi-permeable polymer allows the two-way transfer of molecules such as oxygen, nutrients, and growth factors. The main advantages of cell encapsulation technology include controlling the problems involved in transplanting rejection in tissue engineering applications and reducing the long-term need for immunosuppressive drugs following organ transplantation to eliminate the side effects. Cell-laden microgels can also be used in 3D cell cultures, wound healing, and cancerous clusters for drug testing. Since cell encapsulation is used for different purposes, several techniques have been developed to encapsulate cells. Droplet-based microfluidics is one of the most valuable techniques in cell encapsulating. This study aimed to review the geometries and the mechanisms proposed in microfluidic systems to precisely control cell-laden microgels production with different biopolymers. We also focused on alginate gelation techniques due to their essential role in cell encapsulation applications. Finally, some applications of these microgels and researches will be explored.

Stimuli-Responsive Hydrogels for Local Post-Surgical Drug Delivery.

Currently, surgical operations, followed by systemic drug delivery, are the prevailing treatment modality for most diseases, including cancers and trauma-based injuries. Although effective to some extent, the side effects of surgery include inflammation, pain, a lower rate of tissue regeneration, disease recurrence, and the non-specific toxicity of chemotherapies, which remain significant clinical challenges. The localized delivery of therapeutics has recently emerged as an alternative to systemic therapy, which not only allows the delivery of higher doses of therapeutic agents to the surgical site, but also enables overcoming post-surgical complications, such as infections, inflammations, and pain. Due to the limitations of the current drug delivery systems, and an increasing clinical need for disease-specific drug release systems, hydrogels have attracted considerable interest, due to their unique properties, including a high capacity for drug loading, as well as a sustained release profile. Hydrogels can be used as local drug performance carriers as a means for diminishing the side effects of current systemic drug delivery methods and are suitable for the majority of surgery-based injuries. This work summarizes recent advances in hydrogel-based drug delivery systems (DDSs), including formulations such as implantable, injectable, and sprayable hydrogels, with a particular emphasis on stimuli-responsive materials. Moreover, clinical applications and future opportunities for this type of post-surgery treatment are also highlighted.