Retraction Note: Weather indicators and improving air quality in association with COVID-19 pandemic in India.

[This retracts the article DOI: 10.1007/s00500-021-06012-9.].

Weather indicators and improving air quality in association with COVID-19 pandemic in India.

The COVID-19 pandemic enforced nationwide lockdown, which has restricted human activities from March 24 to May 3, 2020, resulted in an improved air quality across India. The present research investigates the connection between COVID-19 pandemic-imposed lockdown and its relation to the present air quality in India; besides, relationship between climate variables and daily new affected cases of Coronavirus and mortality in India during the this period has also been examined. The selected seven air quality pollutant parameters (PM10, PM2.5, CO, NO2, SO2, NH3, and O3) at 223 monitoring stations and temperature recorded in New Delhi were used to investigate the spatial pattern of air quality throughout the lockdown. The results showed that the air quality has improved across the country and average temperature and maximum temperature were connected to the outbreak of the COVID-19 pandemic. This outcomes indicates that there is no such relation between climatic parameters and outbreak and its associated mortality. This study will assist the policy maker, researcher, urban planner, and health expert to make suitable strategies against the spreading of COVID-19 in India and abroad. Supplementary Information: The online version contains supplementary material available at 10.1007/s00500-021-06012-9.

COVID-19 strict lockdown impact on urban air quality and atmospheric temperature in four megacities of India.

COVID-19 pandemic has forced to lockdown entire India starting from 24th March 2020 to 14th April 2020 (first phase), extended up to 3rd May 2020 (second phase), and further extended up to 17th May 2020 (third phase) with limited relaxation in non-hotspot areas. This strict lockdown has severely curtailed human activity across India. Here, aerosol concentrations of particular matters (PM) i.e., PM10, PM2.5, carbon monoxide (CO), nitrogen dioxide (NO2), sulphur dioxide (SO2), ammonia (NH3) and ozone (O3), and associated temperature fluctuation in four megacities (Delhi, Mumbai, Kolkata, and Chennai) from different regions of India were investigated. In this pandemic period, air temperature of Delhi, Kolkata, Mumbai and Chennai has decreased about 3 °C, 2.5 °C, 2 °C and 2 °C respectively. Compared to previous years and pre-lockdown period, air pollutants level and aerosol concentration (-41.91%, -37.13%, -54.94% and -46.79% respectively for Delhi, Mumbai, Kolkata and Chennai) in these four megacities has improved drastically during this lockdown period. Emission of PM2.5 has experienced the highest decrease in these megacities, which directly shows the positive impact of restricted vehicular movement. Restricted emissions produce encouraging results in terms of urban air quality and temperature, which may encourage policymakers to consider it in terms of environmental sustainability.

Have any effect of COVID-19 lockdown on environmental sustainability? A study from most polluted metropolitan area of India.

The long-term lockdown due to COVID-19 has beneficial impact on the natural environment. India has enforced a lockdown on 24th March 2020 and was subsequently extended in various phases. The lockdown due to the sudden spurt of the COVID-19 pandemic has shown a significant decline in concentration of air pollutants across India. The present article dealt with scenarios of air quality concentration of air pollutants, and effect on climatic variability during the COVID-19 lockdown period in Kolkata Metropolitan Area, India. The result showed that the air pollutants are significantly reduced and the air quality index (AQI) was improved during the lockdown months. Aerosol concentrations decreased by - 54.94% from the period of pre-lockdown. The major air pollutants like particulate matters (PM2.5, PM10), sulphur dioxide (SO2), carbon monoxide (CO) and Ozone (O3) were observed the maximum reduction ( - 40 to - 60%) in the COVID-19 lockdown period. The AQI has been improved by 54.94% in the lockdown period. On the other hand, Sen's slope rank and the Mann-Kendal trend test showed the daily decreased of air pollutants rate is - 0.051 to - 1.586 µg /m3. The increasing trend of daily minimum, average, and maximum temperature from the month of March to May in this year (2020s) are 0.091, 0.118, and 0.106 °C which is lowest than the 2016s to 2019s trend. Therefore, this research has an enormous opportunity to explain the effects of the lockdown on air quality and climate variability, and it can also be helpful for policymakers and decision-makers to enact appropriate measures to control air pollution.

Improvement in ambient-air-quality reduced temperature during the COVID-19 lockdown period in India.

The COVID-19 pandemic forced India as a whole to lockdown from 24 March 2020 to 14 April 2020 (first phase), extended to 3 May 2020 (second phase) and further extended to 17 May 2020 (third phase) and 31 May 2020 (fourth phase) with only some limited relaxation in non-hot spot areas. This lockdown has strictly controlled human activities in the entire India. Although this long lockdown has had a serious impact on the social and economic fronts, it has many positive impacts on environment. During this lockdown phase, a drastic fall in emissions of major pollutants has been observed throughout all the parts of India. Therefore, in this research study we have tried to establish a relationship among the fall in emission of pollutants and their impact on reducing regional temperature. This analysis was tested through the application of Mann-Kendall and Sen's slope statistical index with air quality index and temperature data for several stations across the country, during the lockdown period. After the analysis, it has been observed that daily emissions of pollutants (PM10, PM2.5, CO, NO2, SO2 and NH3) decreased by - 1- - 2%, allowing to reduce the average daily temperature by 0.3 °C compared with the year of 2019. Moreover, this lockdown period reduces overall emissions of pollutants by - 51- - 72% on an average and hence decreases the average monthly temperature by 2 °C. The same findings have been found in the four megacities in India, i.e., Delhi, Kolkata, Mumbai and Chennai; the rate of temperature fall in the aforementioned megacities is close to 3 °C, 2.5 °C, 2 °C and 2 °C, respectively. It is a clear indicator that a major change occurs in air quality, and as a result it reduced lower atmospheric temperature due to the effect of lockdown. It is also a clear indicator that a major change in air quality and favorable temperature can be expected if the strict implementations of several pollution management measures have been implemented by the concern authority in the coming years.

Significant decrease of lightning activities during COVID-19 lockdown period over Kolkata megacity in India.

The outbreak of COVID-19 has now created the largest pandemic and the World health organization (WHO) has declared social distancing as the key precaution to confront such type of infections. Most of the countries have taken protective measures by the nationwide lockdown. The purpose of this study is to understand the effect of lockdown on air pollutants and to analyze pre-monsoon (April and May) cloud-to-ground and inter-cloud lightning activity in relation to air pollutants i.e. suspended Particulate matter (PM10), Nitrogen dioxides (NO2) Sulfur dioxide (SO2), Ozone (O3) and Aerosol concentration (AC) in a polluted tropical urban megacities like Kolkata. After the strict lockdown the pollutants rate has reduced by more than 40% from the pre-lockdown period in the Kolkata megacity. So, decreases of PM10, NO2, SO2, O3 and AC have a greater effect on cloud lightning flashes in the pre-monsoon period. In the previous year (2019), the pre-monsoon average result shows a strong positive relation between the lightning and air pollutants; PM10 (R2 = 0.63), NO2 (R2 = 0.63), SO2 (R2 = 0.76), O3 (R2 = 0.68) and AC (R2 = 0.83). The association was relatively low during the lock-down period (pre-monsoon 2020) and the R2 values were 0.62, 0.60, 0.71, 0.64 and 0.80 respectively. Another thing is that the pre-monsoon (2020) lightning strikes decreased by 49.16% compared to the average of previous years (2010 to 2019). The overall study shows that the reduction of surface pollution in the thunderstorm environment is strongly related to the reduction of lightning activity where PM10 and AC are the key pollutants in the Kolkata megacity.

Understanding the spatio-temporal pattern of COVID-19 outbreak in India using GIS and India's response in managing the pandemic.

Due to the outbreak of Coronavirus, humans all over the world are facing several health problems. The present study has explored the spatio-temporal pattern of Coronavirus spread in India including spatial clustering, identification of hotspot, spatial heterogeneity, and homogeneity, spatial trend, and direction of COVID-19 cases using spatial statistical analysis during the period of 30 January to 20 June 2020. Besides, the polynomial regression model has been used for predictions of COVID-19 affected population and related deaths. The study found positive spatial heterogeneity in COVID-19 cases in India. The study has also identified 17 epicentres across the country with high incidence rates. The directional distribution of ellipse polygon shows that the spread of COVID-19 now trending towards the east but the concentration of cases is mainly in the western part of the country. The country's trend of COVID-19 follows a fourth-order polynomial growth and is characterized by an increasing trend. The prediction results show that as on 14 October India will reach 14,660,400 COVID-19 cases and the death toll will cross 152,945. Therefore, a "space-specific" policy strategy would be a more suitable strategy for reducing the spatial spread of the virus in India. Moreover, the study has broadly found out seven sectors, where the Government of India lacks in terms of confronting the ongoing pandemic. The study has also recommended some appropriate policies which would be immensely useful for the administration to initiate strategic planning.

Debido al brote del Coronavirus, los humanos de todo el mundo se enfrentan a varios problemas de salud. En el presente estudio se han explorado las pautas espacio­temporales de la propagación del Coronavirus en la India, entre ellas la agrupación espacial, la identificación de focos, la heterogeneidad espacial y la homogeneidad, la tendencia espacial y la dirección de los casos de COVID­19, usando un análisis estadístico espacial para el período entre el 30 de enero y el 20 de junio de 2020. Además, se ha utilizado el modelo de regresión polinómica para las predicciones de la población afectada por COVID­19 y las muertes relacionadas. El estudio encontró una heterogeneidad espacial positiva en los casos de COVID­19 en la India. También identificó 17 epicentros en todo el país con altas tasas de incidencia. La distribución direccional del polígono de la elipse muestra que la propagación de COVID­19 ahora tiende hacia el este, pero la concentración de casos se encuentra principalmente en la parte occidental del país. La tendencia del país para COVID­19 sigue un crecimiento polinómico de cuarto orden y se caracteriza por una tendencia al alza. Los resultados de la predicción muestran que, a 14 de octubre, la India alcanzará 14.660.400 casos de COVID­19 y el número de muertes sobrepasará las 152.945. Por consiguiente, una estrategia política "espacialmente específica" sería una estrategia más adecuada para reducir la propagación espacial del virus en la India. Además, el estudio ha descubierto en general siete sectores en los que el Gobierno de la India carece de medios para hacer frente a la pandemia. En el estudio también se recomiendan algunas políticas apropiadas que serían inmensamente útiles para que la administración inicie una planificación estratégica.

Atorvastatin induced hepatic oxidative stress and apoptotic damage via MAPKs, mitochondria, calpain and caspase12 dependent pathways.

Atorvastatin (ATO), a 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor, is used widely for the treatment of hypercholesterolemia and hypertriglyceridemia. Application of this drug has now been made somehow limited because of ATO associated several acute and chronic side effects. The present study has been carried out to investigate the dose-dependent hepatic tissue toxicity in ATO induced oxidative impairment and cell death in mice. Administration of ATO enhanced ALT, ALP level, increased reactive oxygen species (ROS) production and altered the pro oxidant-antioxidant status of liver by reducing intracellular GSH level, anti-oxidant enzymes activities and increasing intracellular lipid peroxidation. Our experimental evidence suggests that ATO markedly decreased mitochondrial membrane potential, disturbed the Bcl-2 family protein balance, enhanced cytochrome c release in the cytosol, increased the levels of Apaf1, caspase-9, -3, cleaved PARP protein and ultimately led to apoptotic cell death. Besides, ATO distinctly increased the phosphorylation of p38, JNK, and ERK MAPKs, enhanced Caspase12 and calpain level. Histological studies also support the dose-dependent toxic effect of ATO in these organs pathophysiology. These results reveal that ATO induces hepatic tissue toxicity via MAPKs, mitochondria and ER dependent signaling pathway, in which calcium ions and ROS act as the pivotal mediators of the apoptotic signaling.

Nanotoxicity: oxidative stress mediated toxicity of metal and metal oxide nanoparticles.

Metal and metal oxide nanoparticles are often used as industrial catalysts or to improve product's functional properties. Recent advanced nanotechnology have been expected to be used in various fields, ranging from sensors, environmental remediation to biomedicine, medical biology and imaging, etc. However, the growing use of nanoparticles has led to their release into environment and increased levels of these particles at nearby sites or the surroundings of their manufacturing factories become obvious. The toxicity of metal and metal oxide nanoparticles on humans, animals, and certainly to the environment has become a major concern to our community. However, controversies still remain with respect to the toxic effects and the mechanisms of these nanoparticles. The scientific community now feels that an understanding of the toxic effects is necessary to handle these nanoparticles and their use. A new discipline, named nanotoxicology, has therefore been developed that basically refers to the study of the interactions of nanoparticles with biological systems and also measures the toxicity level related to human health. Nanoparticles usually generate reactive oxygen species to a greater extent than micro-sized particles resulting in increased pro-inflammatory reactions and oxidative stress via intracellular signaling pathways. In this review, we mainly focus on the routes of exposure of some metal and metal oxide nanoparticles and how these nanoparticles affect us or broadly the cells of our organs. We would also like to discuss the responsible mechanism(s) of the nanoparticle-induced reactive oxygen species mediated organ pathophysiology. A brief introduction of the characterization and application of these nanoparticles has also been included in the article.

D(+) galactosamine induced oxidative and nitrosative stress-mediated renal damage in rats via NF-κB and inducible nitric oxide synthase (iNOS) pathways is ameliorated by a polyphenol xanthone, mangiferin.

The present study investigated the possible protective effect of mangiferin against D(+) galactosamine (DGal)-induced nephrotoxicity. DGal intoxication increased reactive oxygen species (ROS), reactive nitrogen species and tumor necrosis factor-α (TNF-α) production and disturbed the antioxidant machineries in the kidney tissue. Mangiferin treatment post to DGal exposure reduced all these DGal-induced adverse effects. Signal transduction studies showed that DGal significantly increased the protein expression of Bax, cytochrome c, caspase 3/9 and inducible nitric oxide synthase (iNOS) in the cytosol and NF-κB in nuclear fraction. The same exposure, on the other hand, reduced the protein expression of Bcl-2 in the cytosol. Mangiferin treatment could, however, reduce the DGal-induced up-regulation of cytochrome c, NF-κB, iNOS, caspase 3/9 and alter the reciprocal regulation of Bcl-2 family proteins. Histological studies also revealed the nephroprotective effect of mangiferin against DGal induced nephrotoxicity. Combining, results suggest that mangiferin protects rat's kidney in DGal-induced oxidative/nitrosative stress and acute nephrotoxicity via its antioxidant activities.

Contribution of nano-copper particles to in vivo liver dysfunction and cellular damage: role of IκBα/NF-κB, MAPKs and mitochondrial signal.

The present study investigated the oxidative stress responsive cell signaling in nano-copper-induced hepatic dysfunction and cell death. Exposure to nano-copper (18 nm) dose-dependently (200-600 mg/kg bw) reduced the hepatic index, caused oxidative stress and led to hepatic dysfunction. Nano-copper burden also increased the transcriptional activity of NF-κB, up-regulated the expression of phosphorylated p38, ERK1/2 and caused the reciprocal regulation of Bcl-2 family proteins, disruption of mitochondrial membrane potential, release of cytochrome C, formation of apoptosome and activation of caspase 3. DAPI staining, immunofluorescence study, FACS analysis and histological findings also support this observation. Soluble copper (Cu(+2), 110 mg/kg bw)-exposed animals were used as a positive control. Different doses of particulate and soluble forms were used in the study because of different LD(50) values. The results suggest that nano-copper induces hepatic dysfunction and cell death via the oxidative stress-dependent signaling cascades and mitochondrial event.

Protective role of a coumarin-derived schiff base scaffold against tertiary butyl hydroperoxide (TBHP)-induced oxidative impairment and cell death via MAPKs, NF-κB and mitochondria-dependent pathways.

The present study investigated the antioxidant signalling mechanism of a coumarin-derived schiff base (CSB) scaffold against tert-butylhydroperoxide (TBHP) induced oxidative insult in murine hepatocytes. CSB possesses DPPH and other free radical scavenging activities. TBHP reduced cell viability and intracellular antioxidant status accompanied by an increase in intracellular ROS production in hepatocytes. TBHP also activated phospho-ERK1/2, phospho-p38 and NF-κB, altered the Bcl-2/Bad ratio, reduced mitochondrial membrane potential, released cytochrome C and activated caspase 3, suggesting that TBHP induced oxidative stress responsive cell death via apoptotic pathway. FACS analysis and DNA fragmentation studies also confirmed the apoptotic cell death in TBHP exposed hepatocytes. Treatment with CSB effectively reduced these adverse effects by preventing the oxidative insult, alteration in the redox-sensitive signalling cascades and mitochondrial events. Combining, results suggest that antioxidant property of CSB make the molecule to be a potential protective measure against oxidative insult, cytotoxicity and cell death.

Shape transition in ZnO nanostructures and its effect on blue-green photoluminescence.

We report that ZnO nanostructures synthesized by a chemical route undergo a shape transition at ∼20 nm from spherical to hexagonal morphology thereby changing the spectral components of the blue-green emission. Spherically shaped nanocrystals (size range 11-18 nm) show emission in the range of 555-564 nm and the emission shifts to the longer wavelength as the size increases. On the other hand, rods and hexagonal platelets (size range 20-85 nm), which are the equilibrium morphology after the shape transition, show an emission near 465-500 nm which shifts to shorter wavelength as the size increases. The shape transition also leads to relaxation of microstrain in the system. Our analysis shows that the visible emission originates from a defect layer on the nanostructure surface which is affected by the shape transition. The change in the spectral component of the blue-green emission on change of shape has been explained as arising from band bending due to a depletion layer in smaller spherical particles which is absent in the larger particles with flat faces.

Ultrafast photoinduced deligation and ligation dynamics: DCM in micelle and micelle-enzyme complex.

We report studies on diffusion controlled deligation and ligation dynamics of a probe ligand 4-(dicyanomethylene)-2-methyl-6-(p-dimethylamino-styryl) 4H-pyran (DCM) with cationic cetyltrimethylammonium bromide (CTAB) micelles. In order to investigate the effect of spatial heterogeneity on the dynamics we study the DCM labeled micelle upon complexation with an enzyme alpha-chymotrypsin (CHT). The variation of fluorescence line-width (Gamma(t)) of DCM in the complex and also in the micelle indicates the diffusion dynamics of DCM through various environments of different polarities. The temporal behavior of Gamma(t) reveals that at 50 mM CTAB concentration the excited DCM traverses 6.5 Angstrom distance from the surface of a host micelle (deligation) before entering to a stern layer of another adjacent micelle (ligation). From neutron scattering experiment the distance 6.5 Angstrom is found to be the thickness of a stern layer of CTAB micelle. No indication of ligation has been found at 2 mM CTAB concentration as the intermicellar distance is estimated to be very large (416 Angstrom) compared to the previous case. The dynamical behavior of Gamma(t) is also indicative of significantly slower diffusion of the ligand molecules (DCM) at the surface of the micelle in presence and absence of the enzyme compared to that in the bulk buffer. We have also studied the dynamics of solvation and local geometrical restriction on the probe DCM at the micellar surface with and without CHT. With picosecond time resolution, we found time constants of the solvation relaxation processes of the DCM labeled enzyme-micelle complex to be 230 ps (45%) and 870 ps (55%), which were comparable to those of the micelle without the enzyme. The time dependent anisotropy revealing local orientational motions of the probe in the complex was also found to be similar to that of DCM at the micellar surface in absence of CHT. These studies attempt to link the dynamical features for insight into the ligand mediated intercellular communication and the biological function of the enzyme alpha-chymotrypsin upon complexation with the CTAB micelle.

Ultrafast surface solvation dynamics and functionality of an enzyme alpha-chymotrypsin upon interfacial binding to a cationic micelle.

In this contribution we report studies on enzymatic activity of alpha-chymotrypsin (CHT) upon complexation with cationic cetyltrimethylammonium bromide (CTAB) micelle. With picosecond time resolution, we examined solvation dynamics at the interface of CHT-micelle complex, and rigidity of the binding. We have used 5-(dimethyl amino) naphthalene-1-sulfonyl chloride (dansyl chloride; DC) that is covalently attached to the enzyme at the surface sites. The solvation processes at the surface of CHT in buffer solution are found to be mostly in the sub-50 ps time scale. However, at the interface the solvation correlation function decays with time constant 150 ps (65%) and 500 ps (35%), which is significantly different from those found at the enzyme and micellar surfaces. The binding structure of the enzyme-micelle complex was examined by local orientational motion of the probe DC and compared with the case without micelle. The orientational dynamics of the probe DC in the complex reveals a structural perturbation at the surface sites of CHT upon complexation, consistent with other reported structural studies. We also found possible entanglement of charge transfer dynamics of the probe DC on the measured solvation processes by using time-resolved area normalized emission spectroscopic technique. The interfacial solvation process and complex rigidity elucidate the strong recognition mechanism between CHT and the micelle, which is important to understand the biological function of CHT upon complexation with the micelle.

Ultrafast relaxation dynamics of a biologically relevant probe dansyl at the micellar surface.

We report picosecond-resolved measurement of the fluorescence of a well-known biologically relevant probe, dansyl chromophore at the surface of a cationic micelle (cetyltrimethylammonium bromide, CTAB). The dansyl chromophore has environmentally sensitive fluorescence quantum yields and emission maxima, along with large Stokes shift. In order to study the solvation dynamics of the micellar environment, we measured the fluorescence of dansyl chromophore attached to the micellar surface. The fluorescence transients were observed to decay (with time constant approximately 350 ps) in the blue end and rise with similar timescale in the red end, indicative of solvation dynamics of the environment. The solvation correlation function is measured to decay with time constant 338 ps, which is much slower than that of ordinary bulk water. Time-resolved anisotropy of the dansyl chromophore shows a bi-exponential decay with time constants 413 ps (23%) and 1.3 ns (77%), which is considerably slower than that in free solvents revealing the rigidity of the dansyl-micelle complex. Time-resolved area-normalized emission spectroscopic (TRANES) analysis of the time dependent emission spectra of the dansyl chromophore in the micellar environment shows an isoemissive point at 21066 cm-1. This indicates the fluorescence of the chromophore contains emission from two kinds of excited states namely locally excited state (prior to charge transfer) and charge transfer state. The nature of the solvation dynamics in the micellar environments is therefore explored from the time-resolved anisotropy measurement coupled with the TRANES analysis of the fluorescence transients. The time scale of the solvation is important for the mechanism of molecular recognition.