Asunto(s)
Proteínas Fimbrias/genética , Vibrio cholerae O1/genética , Vibrio cholerae O1/aislamiento & purificación , Técnicas Bacteriológicas/métodos , ADN Bacteriano/química , ADN Bacteriano/genética , Genotipo , Humanos , India , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADNRESUMEN
A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of ctxB alleles. Our retrospective study using this newly developed PCR showed that Haitian ctxB first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant strains isolated recently that was distinct from the PFGE pattern of the strains carrying classical ctxB that closely matched the 2006 to 2007 variant strains.
Asunto(s)
Toxina del Cólera/genética , Cólera/microbiología , Reacción en Cadena de la Polimerasa/métodos , Vibrio cholerae O1/clasificación , Vibrio cholerae O1/genética , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , Electroforesis en Gel de Campo Pulsado , Humanos , India , Datos de Secuencia Molecular , Tipificación Molecular , Estudios Retrospectivos , Análisis de Secuencia de ADN , Vibrio cholerae O1/aislamiento & purificaciónRESUMEN
This study examined established enteric pathogens, Arcobacter species and enterotoxigenic Bacteroides fragilis (ETBF), in 201 U.S. and European travelers with acute diarrhea acquired in Mexico, Guatemala, and India. Arcobacter butzleri and ETBF were detected in 8% and 7% of diarrhea cases, respectively.