Attenuation and soil biodegradation of fungicides by using vegetated buffer strips in vineyards during a simulated rainfall-runoff event.

Rainfall-runoff events occurring in vineyard fields can result in pesticide ground losses and the subsequent pollution of surface water bodies, derivate from the crop protection spray applications. In this study, the capacity of vegetated buffer strips (BS) to prevent surface water pollution due to the application of five fungicide products typically used in vineyards (copper, dimethomorph, oxathiapiprolin, zoxamide, acibenzolar-s-methyl, and laminarin) following a simulated run-off event has been assessed, and compared to that from a bare ground soil (BG). Two strips (5 m in length, each), one with vegetation and the other without were built up, and two different experiments were performed, a runoff event and a soil fungicide degradation kinetic evaluation. The runoff results show that fungicide mass retention in the strips ranged from 73 to 98% and that the presence of vegetation in BS increased the fungicide mass retention in the strips by almost 10% (on average) in comparison to the unvegetated strip. Moreover, soil degradation studies highlighted that the presence of vegetation reduces significantly the half-time life of almost all the studied fungicides by 55%, on average. Eight fungicide transformation products (TPs) were identified following a runoff event in the soil strips, but the abundance of these TPs was up to 78% lower in vegetated strips. These results highlight the effectiveness of using vegetated buffer zones in vineyards to protect aquatic ecosystem pollution.

Multicriteria analysis of critical areas for restoration in a semiarid landscape: A comparison between stakeholder groups.

In landscape-scale ecological restoration, there is an urgent need to develop participatory systematic planning strategies and prioritization schemes that are operational under current technical and legal constraints. Different stakeholder groups may differ in their choice of criteria to define critical areas for restoration. Analyzing the correspondence between stakeholder characteristics and their expressed preferences is key to understand their values and facilitate consensus among the different groups. We analyzed the participatory identification of critical areas for restoration in a Mediterranean semiarid landscape of southeastern Spain by means of two Spatial Multicriteria Analyses. The first one included 33 ecological and socioeconomic prioritization criteria. The second included 24 ecosystem services. Prioritization criteria and services and their weights were based on the preferences of 46 stakeholders. We distinguished three stakeholder groups, according to their approach to ecological restoration. Stakeholders showed similarities regarding the most important criteria and services assessed. Yet, we found contrasted opinions between the group labeled as Biodiversity, who showed preference for Regulating Services and Ecosystem Functions, and the two groups labeled as Environment, and Agriculture & other occupations who assigned the highest importance to Provisioning and Cultural Services, along with highly Anthropized Environments. Maps integrating criteria and services weighted by the different groups of stakeholders were largely coincident, because of their overall agreement and the high number of criteria and services included in the analysis. Our approach allowed the identification of consensual critical areas for restoration, which were mainly covered by shrublands and rainfed crops, and mostly characterized by low to medium supply of ecosystem services. Our study emphasizes the need to recognize and integrate different social perspectives when identifying critical areas for restoration and highlights the importance of using complementary approaches as decision-making support tools to define these areas.

Use of cover crops in vineyards to prevent groundwater pollution by copper and organic fungicides. Soil column studies.

Several fungicides, such as copper and organic products (synthetic or natural), are currently being used in vineyards to control downy mildew (Plasmopara viticola) resulting in soil, surface water, and groundwater pollution. This study aims to assess the effectiveness of using cover crops as an agricultural practice in vineyards to protect soil and groundwater pollution. For that purpose, we performed different soil column studies to quantify soil leaching of selected fungicides (copper, dimethomorph, oxathiapiprolin, zoxamide, acibenzolar-s-methyl, and laminarin) following a rainfall event after a conventional fungicide vineyard application. Two types of vineyard soils (loam and sandy-loam soil textures) and three ground covers (bare ground, monoculture cover, and polyculture cover) were assessed. These studies were completed with hydroponic assays to check the effectiveness of cover roots in the fungicide degradation. Mass balance results show that whereas 3 fungicides (Cu, zoxamide, and dimethomorph) were leached through sandy soil columns, only copper was leached from loam soil columns. The effect of cover crops was only significant for Cu and zoxamide when fungicides were applied 24 h before the rain event, reducing the fungicide leaching by 30%. Hydroponic studies showed that cover roots enhanced the kinetic rates of almost all tested fungicides by 5-467%, suggesting that they are relevant to improving the degradation of fungicides in the soil column. These results are relevant to drawing up recommendations on the use of cover crops to protect soil and groundwater pollution by fungicides.

Pesticide dose based on canopy characteristics in apple trees: Reducing environmental risk by reducing the amount of pesticide while maintaining pest and disease control efficacy.

Although many different designed air-assisted sprayers can be used for pesticide application in apple orchards, the lack of adequate adjustments according to specific crop characteristics leads to application inefficiencies and failures. To evaluate the spray coverage and biological efficacy of different application techniques combined with an alternative dosage adjustment based on tree row volume (TRV), field tests with five different techniques were carried out at three crop stages on a commercial apple orchard. The results showed that conventional mist-blower with a high application volume (800 L ha-1) exhibited an excessive coverage with a high risk of contamination at the early crop stage (BBCH19), whereas other treatments using different application techniques, with a reduced volume rate and pesticide dose of 75%, were equivalent with good uniformity, revealing the great importance of suitable adjustment for the sprayers. For the middle and late stages (BBCH64 and 75), the orchard sprayer equipped with vertical booms provided the maximum coverage, and the pneumatic sprayer achieved significantly higher impacts density, which revealed their advantages and high efficiency for dense apple trees. The newly developed multi-fan sprayer and pneumatic sprayer achieved consistent coverage during the entire crop stage, independent of the changes in canopy structure (TRV). This indicates that a suitable setting and adjustment of the sprayer can contribute to a consistent spray quality. In general, benefiting from these new spraying technologies, an average reduction of 60.7% in pesticide dose and volume rate were achieved within the entire season, maintaining the same threshold of pest and disease control as that of the higher reference dose normally applied. These results demonstrate the importance of an alternative dose adjustment method to meet the requirements of the Farm to Fork strategy.

Applications of Carbon Dots for the Photocatalytic and Electrocatalytic Reduction of CO2.

The photocatalytic and electrocatalytic conversion of CO2 has the potential to provide valuable products, such as chemicals or fuels of interest, at low cost while maintaining a circular carbon cycle. In this context, carbon dots possess optical and electrochemical properties that make them suitable candidates to participate in the reaction, either as a single component or forming part of more elaborate catalytic systems. In this review, we describe several strategies where the carbon dots participate, both with amorphous and graphitic structures, in the photocatalysis or electrochemical catalysis of CO2 to provide different carbon-containing products of interest. The role of the carbon dots is analyzed as a function of their redox and light absorption characteristics and their complementarity with other known catalytic systems. Moreover, detailed information about synthetic procedures is also reviewed.

Analysis of the Oxygen Passivation Effects on MAPbI3 and MAPbBr3 in Fresh and Aged Solar Cells by the Transient Photovoltage Technique.

Previous studies have revealed that for some perovskite compositions, power conversion efficiencies (PCEs) improved after storing the devices in different ambient conditions. With the aim of better understanding such improvements, we focus our attention on the carrier/ionic dynamic kinetics of fresh and aged PSCs with different perovskite compositions (MAPbI3 and MAPbBr3 ) and using spiro-OMeTAD as HTM. For that, we use transient photovoltage (TPV), a technique used to analyse the different recombination kinetics at equilibrium and at different illumination times. We observe that the aging treatment causes significant changes on the kinetics behaviour for bromide-based devices, resulting in a positive influence on the cell performance (from 3.5 % to 6.1 % PCE, in reverse scan). However, the kinetics for those iodide-based perovskite solar cells remains unchangeable (from 16.3 % to 15.0 % PCE, in reverse scan).

Assessment of Vineyard Canopy Characteristics from Vigour Maps Obtained Using UAV and Satellite Imagery.

Canopy characterisation is a key factor for the success and efficiency of the pesticide application process in vineyards. Canopy measurements to determine the optimal volume rate are currently conducted manually, which is time-consuming and limits the adoption of precise methods for volume rate selection. Therefore, automated methods for canopy characterisation must be established using a rapid and reliable technology capable of providing precise information about crop structure. This research providedregression models for obtaining canopy characteristics of vineyards from unmanned aerial vehicle (UAV) and satellite images collected in three significant growth stages. Between 2018 and 2019, a total of 1400 vines were characterised manually and remotely using a UAV and a satellite-based technology. The information collected from the sampled vines was analysed by two different procedures. First, a linear relationship between the manual and remote sensing data was investigated considering every single vine as a data point. Second, the vines were clustered based on three vigour levels in the parcel, and regression models were fitted to the average values of the ground-based and remote sensing-estimated canopy parameters. Remote sensing could detect the changes in canopy characteristics associated with vegetation growth. The combination of normalised differential vegetation index (NDVI) and projected area extracted from the UAV images is correlated with the tree row volume (TRV) when raw point data were used. This relationship was improved and extended to canopy height, width, leaf wall area, and TRV when the data were clustered. Similarly, satellite-based NDVI yielded moderate coefficients of determination for canopy width with raw point data, and for canopy width, height, and TRV when the vines were clustered according to the vigour. The proposed approach should facilitate the estimation of canopy characteristics in each area of a field using a cost-effective, simple, and reliable technology, allowing variable rate application in vineyards.

Relative efficiencies of experimental and conventional foliar sprayers and assessment of optimal LWA spray volumes in trellised wine grapes.

BACKGROUND: Leaf wall area (LWA) has been proposed as an appropriate dose expression for field testing of plant protection products (PPPs) applied via foliar spray in trellised grapes. However, its efficiency could change depending on the characteristics of the crop or the pesticide application equipment (PAE). Herein, three spray technologies were evaluated. A traditional air-assisted tractor-mounted sprayer was compared with two portable knapsack sprayers: a backpack mistblower and a backpack hydraulic sprayer. Trials were conducted in trellised wine grapes at three selected crop stages (BBCH 55, 65, 75) covering the main period of canopy development. In each canopy stage, leaf deposition and coverage were sampled for each technology. The tractor-mounted sprayer was working at 200 L ha-1 of LWA spray volume for the earliest stage and 370 L ha-1 for the other two. Three higher volume rates were used for backpack sprayers up to 800 and 1250 L ha-1 for the mistblower and the hydraulic system, respectively. RESULTS: Optimal LWA spray volumes differed among application devices in terms of efficiency and uniformity of deposition on the canopy. The efficiency of each spray application also was conditioned by the spray volume. CONCLUSION: LWA is useful for defining optimal spray volumes in trellised grapes. However, both canopy density and spray technology should be considered to assist this process. Field testing of PPPs and subsequent label recommendations should consider the relative efficiencies of corresponding experimental and conventional spray technologies. © 2021 Society of Chemical Industry.

The roles of strawberry and honey phytochemicals on human health: A possible clue on the molecular mechanisms involved in the prevention of oxidative stress and inflammation.

BACKGROUND: Oxidative stress and inflammation contribute to the etiopathogenesis of several human chronic diseases, such as cancer, diabetes, cardiovascular diseases and metabolic syndrome. Besides classic stimuli, such as reactive oxidant species, endotoxins (i.e., bacteria lipopolysaccharide), cytokines or carcinogens, oxidative stress and inflammation can be triggered by a poor diet and an excess of body fat and energy intake. Strawberry and honey are common rich sources of nutrients and bioactive compounds, widely studied for their roles exerted in health maintenance and disease prevention. PURPOSE: This review aims to summarize and update the effects of strawberry and honey against oxidative stress and inflammation, with emphasis on metabolism and on the main molecular mechanisms involved in these effects. METHODS: A wide range of literature, published in the last 10 years, elucidating the effects of strawberry and honey in preventing oxidative stress and inflammation both in vitro (whole matrix and digested fractions) and in vivo was collected from online electronic databases (PubMed, Scopus and Web of Science) and reviewed. RESULTS: Strawberry and honey polyphenols may potentially prevent the chronic diseases related to oxidative stress and inflammation. Several in vitro and in vivo studies reported the effects of these foods in suppressing the oxidative stress, by decreasing ROS production and oxidative biomarkers, restoring the antioxidant enzyme activities, ameliorating the mitochondrial antioxidant status and functionality, among others, and the inflammatory process, by modulating the mediators of acute and chronic inflammation essential for the onset of several human diseases. These beneficial properties are mediated in part through their ability to target multiple signaling pathways, such as p38 MAPK, AMPK, PI3K/Akt, NF-κB and Nrf2. CONCLUSIONS: Available scientific literature show that strawberry and honey may be effective in preventing oxidative stress and inflammation. The deep evaluation of the factors that affect their metabolism as well as the assessment of the main molecular mechanisms involved are of extreme importance for the possible therapeutic and preventive benefit against the most common human diseases. However, published literature is still scarce so that deeper studies should be performed in order to evaluate the bioavailability of these food matrices and their effects after digestion.

Potential Health Benefit of Garlic Based on Human Intervention Studies: A Brief Overview.

Garlic is a polyphenolic and organosulfur enriched nutraceutical spice consumed since ancient times. Garlic and its secondary metabolites have shown excellent health-promoting and disease-preventing effects on many human common diseases, such as cancer, cardiovascular and metabolic disorders, blood pressure, and diabetes, through its antioxidant, anti-inflammatory, and lipid-lowering properties, as demonstrated in several in vitro, in vivo, and clinical studies. The present review aims to provide a comprehensive overview on the consumption of garlic, garlic preparation, garlic extract, and garlic extract-derived bioactive constituents on oxidative stress, inflammation, cancer, cardiovascular and metabolic disorders, skin, bone, and other common diseases. Among the 83 human interventional trials considered, the consumption of garlic has been reported to modulate multiple biomarkers of different diseases; in addition, its combination with drugs or other food matrices has been shown to be safe and to prolong their therapeutic effects. The rapid metabolism and poor bioavailability that have limited the therapeutic use of garlic in the last years are also discussed.

Phenolic compounds from Mediterranean foods as nutraceutical tools for the prevention of cancer: The effect of honey polyphenols on colorectal cancer stem-like cells from spheroids.

Chemoresistance and development of relapses are ascribable to a rare cell population of tumour mass: cancer stem cells (CSCs). Targeting CSCs could increase patients' survival rate and it is important to identify molecules that can act on the main pathways of these cells. Natural bioactive compounds, of which Manuka honey (MH) is rich, could be a good opportunity to target them. This work aims to evaluate the effect of MH on CSCs-like from human colorectal carcinoma (HCT-116 cell line) enriched through the in vitro sphere-forming assay. The results showed that MH reduced the volume of the entire culture of spheroids, affecting also their morphological parameters and induced apoptosis and ROS intracellular accumulation in CSCs-like. In addition, MH decreased the mRNA expression of one of ABC transporters (ABCG2) and affected self-renewal ability through the downregulation of the mRNA expression of one of the receptor membranes of Wnt/ß-catenin pathway (Frizzled 7).

Biphasic Computational Fluid Dynamics Modelling of the Mixture in an Agricultural Sprayer Tank.

Agitation inside agricultural sprayer tanks can be studied while using an international standard procedure, based on obtaining internal samples of liquid. However, in practice, this test is not easy to perform. Herein, we propose the explicit study of the mixing procedure with biphasic computer simulations using Computational Fluid Dynamics (CFD). An experimental test was performed on a 3000 L tank of a commercial air-assisted sprayer, with two different agitation system configurations, in order to compare the results of several theoretical physical models of biphasic flows for CFD, both Eulerian and Lagrangian. From the analysis of these theoretical models, we conclude that the Volume of Fluid model is not viable and the Discrete Phase Model produces erroneous results, while the Eulerian and Mixture models can both be useful. However, the results obtained suggest that complex streams generated by real-world agitation systems produce more errors in calculations. Both models can be conducted in the design phase, prior to the implementation of the machine. In addition, the computer simulations allow for researchers to analyse the mixing process in detail, making it possible to evaluate the efficiency of an agitation system according to the time that is required to reach mixture homogeneity.

Assessment of biowaste composting process for industrial support tool development through macro data approach.

This study aims to assess composting efficiency and quality of compost through the study of the parameters of the Catalan Waste Agency (ARC) data-base by developing indicators useful for industrial sector. The study includes 17 composting plants for an 8-years period (2010-2017), the quantities of materials treated and generated in these plants: biowaste, yard trimmings, refuse and compost, as well as chemical characterization of compost: moisture, total organic matter, organic nitrogen, pH, electrical conductivity, self-heating test, pollutants and ammonium. Plant were sorted into 4 size classes depending on size capacity and into 4 technologies employed during thermophilic phase. Different indicators were developed related to improper fraction content, yard trimmings ratio, mass losses, compost production, refuse generation and plant saturation. The main average results indicate that improper fraction is 10%, process losses 68%, refuse generated 25% and saturation 79%. Differences were observed in size and technology; for instance, smaller plants presented lower improper content, refuse and saturation and higher losses while plants with turned windrows during decomposition presented higher improper, yard trimmings ratio and plants with vessel technology showed lower losses and higher saturation. Also, the compost quality is higher if the plant saturation and improper fraction are below 90% and 7%, respectively. The indicators were useful to assess the process and were related to the compost quality obtained.

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey.

: Manuka honey (MH) is a natural food with many beneficial properties to human health, thanks to its high variety of bioactive compounds; however, little is known about its bioaccessibility. The aim of this study was to evaluate and compare the polyphenol compounds, the antioxidant capacity and the anticancer activity of MH subjected to an in vitro gastrointestinal digestion in human HCT-116 colon cancer cells. Raw MH and digested MH (DMH) were assessed for total polyphenols and flavonoids by spectrophotometric and HPLC-ESI-MS/MS analysis, and total antioxidant capacity (TAC) using different methods. Cell viability, intracellular ROS production, apoptosis, cell cycle and colony formation capacity were tested after treatment with MH or DMH. Results showed that total polyphenols, total flavonoids and TAC were significantly (p < 0.05) reduced after in vitro digestion. In addition, MH and DMH at 8, 16 and 24 mg/mL had similar effects in inducing intracellular ROS production and in inhibiting the colon formation ability; MH induced a more marked apoptosis compared to DMH, while cell cycle was blocked in S phase by MH and in Sub G1 phase by DMH. Our results increase knowledge of the effect of gastrointestinal digestion on the biological effect of honey against colorectal cancer.

Resveratrol inhibits the proliferation of melanoma cells by modulating cell cycle.

The aim of this study was to evaluate the inhibitory effects of resveratrol (RSV) in A375 and A431 melanoma cells, by assessing cell viability (CCK-8 assay), apoptosis through flow cytometer and cell morphology, cell cycle assay by flow cytometer and western blot (Cyclin D1, Rac1 and PCDH9). Our results demonstrated that RSV strongly inhibited A375 cells proliferation, by decreasing cell viability, promoting apoptosis and arresting cell cycle. Besides, to clarify the main factor - duration or concentration of RSV, the double variance analysis of independent factors was operated after Bartlett's test for homogeneity by R project. According to the outcomes obtained from statistical analyses, Cyclin D1 and PCDH9 were strongly affected by RSV duration while Rac1 was not influenced. In conclusion, RSV can inhibit A375 proliferation and trigger apoptosis by influencing cell cycle proteins; for these effects, treatment duration of RSV played more important role than concentration.

Application of an Acoustic Doppler Velocimeter to Analyse the Performance of the Hydraulic Agitation System of an Agricultural Sprayer.

An acoustic Doppler velocimeter (ADV) was used to analyse the impact of an agricultural sprayer's agitation system settings on fluid velocities inside the tank. A 3000 L capacity sprayer equipped with a 4-nozzle hydraulic agitation system was used. ADV measurements were carried at 32 points inside the tank under the following settings: circuit pressures of 8, 10, or 12 bar; water level in the tank of 1000, 2000, or 3000 L; 2 or 4 active nozzles. An agitation test with a concentration of 0.4% copper oxychloride was employed to analyse the concentration of active matter as a function of tank fill level and number of active nozzles. All parameters significantly affected the fluid velocity, which increased with increasing pressure, but decreased with increasing water level in the tank and an increased number of active nozzles. Concentration tests showed greater active matter concentrations when higher velocities were recorded by the ADV. The ADV was shown to be a useful tool for the rapid assessment of fluid velocities; in the future, it could be used to validate the design of agitation systems, and to estimate their capacity to ensure an adequate level of active matter concentration in the fluid.

Safety and efficacy of self-administered inhaled loxapine (ADASUVE) in agitated patients outside the hospital setting: protocol for a phase IV, single-arm, open-label trial.

INTRODUCTION: There is a need for fast-acting, non-injection antiagitation treatments that are well tolerated and can be used outside of healthcare facilities. In phase II/III trials, an inhaled formulation of loxapine (ADASUVE®), a well-established, first-generation antipsychotic agent, provided rapid control of mild to moderate agitation in the hospital setting. The present study was designed to investigate the safety and efficacy of inhaled loxapine when self-administered outside the hospital setting. METHODS AND ANALYSIS: This phase IV, multicentre, single-arm, open-label clinical trial is being conducted in five countries in Europe: Spain, Germany, Norway, Romania and Austria. The aim is to include approximately 500 patients with schizophrenia or bipolar disorder who previously received and responded well to inhaled loxapine in the hospital setting. Eligible patients will be followed up for 6 months from baseline. They will be given a 10 mg dose of inhaled loxapine to self-administer outside the hospital setting to treat an agitation episode, should one occur. Patients will also be given a short-acting beta-agonist bronchodilator for treatment of possible severe respiratory side effects. The primary endpoint is incidence of serious adverse events (AEs) and respiratory AEs of special interest related to use of inhaled loxapine outside the hospital setting. Secondary endpoints include incidence of other AEs, Clinical Global Impression-Improvement scores up to 2 hours after self-administration of inhaled loxapine, time to improvement of agitation, patient satisfaction with treatment, treatment outcomes according to agitation severity and concordance between the patient (or a family member/caregiver) and the physician in scoring of agitation severity and the decision to self-administer inhaled loxapine. ETHICS AND DISSEMINATION: The protocol received ethics committee approval in the participating countries between January and August 2016. The results of this study will be disseminated through one or more scientific papers. TRIAL REGISTRATION NUMBER: EudraCT2015-003331-36; NCT02525991; Pre-results.

First attempts to obtain a reference drift curve for traditional olive grove's plantations following ISO 22866.

The current standard for the field measurements of spray drift (ISO 22866) is the only official standard for drift measurements in field conditions for all type of crops, including bushes and trees. A series of field trials following all the requirements established in the standard were arranged in a traditional olive grove in Córdoba (south of Spain). The aims of the study were to evaluate the applicability of the current standard procedure to the particular conditions of traditional olive trees plantations, to evaluate the critical requirements for performing the tests and to obtain a specific drift curve for such as important and specific crop as olive trees in traditional plantations, considering the enormous area covered by this type of crop all around the world. Results showed that the field trials incur a very complex process due to the particular conditions of the crop and the very precise environmental requirements. Furthermore, the trials offered a very low level of repeatability as the drift values varied significantly from one spray application to the next, with the obtained results being closely related to the wind speed, even when considering the standard minimum value of 1 m·s-1. The collector's placement with respect to the position of the isolated trees was determined as being critical since this substantially modifies the ground deposit in the first 5 m. Even though, a new drift curve for olive trees in traditional plantation has been defined, giving an interesting tool for regulatory aspects. Conclusions indicated that a deep review of the official standard is needed to allow its application to the most relevant orchard/fruit crops.

Testing the Suitability of a Terrestrial 2D LiDAR Scanner for Canopy Characterization of Greenhouse Tomato Crops.

Canopy characterization is essential for pesticide dosage adjustment according to vegetation volume and density. It is especially important for fresh exportable vegetables like greenhouse tomatoes. These plants are thin and tall and are planted in pairs, which makes their characterization with electronic methods difficult. Therefore, the accuracy of the terrestrial 2D LiDAR sensor is evaluated for determining canopy parameters related to volume and density and established useful correlations between manual and electronic parameters for leaf area estimation. Experiments were performed in three commercial tomato greenhouses with a paired plantation system. In the electronic characterization, a LiDAR sensor scanned the plant pairs from both sides. The canopy height, canopy width, canopy volume, and leaf area were obtained. From these, other important parameters were calculated, like the tree row volume, leaf wall area, leaf area index, and leaf area density. Manual measurements were found to overestimate the parameters compared with the LiDAR sensor. The canopy volume estimated with the scanner was found to be reliable for estimating the canopy height, volume, and density. Moreover, the LiDAR scanner could assess the high variability in canopy density along rows and hence is an important tool for generating canopy maps.

Spray distribution evaluation of different settings of a hand-held-trolley sprayer used in greenhouse tomato crops.

BACKGROUND: Hand-held-trolley sprayers have recently been promoted to improve spray application techniques in greenhouses in south-eastern Spain. However, certain aspects remain to be improved. A modified hand-held-trolley sprayer was evaluated under two different canopy conditions (high and low canopy density) and with several sprayer settings (nozzle type, air assistance and spray volume). In this study, the deposition, coverage and uniformity of distribution of the spray on the canopy have been assessed. RESULTS: The deposition on leaves was significantly higher when flat-fan nozzles and air assistance were used at both high and low spray volumes. No differences were detected between the reference system at a high spray volume and the modified trolley at a low spray volume. Flat-fan nozzles with air assistance increased penetrability into the canopy. CONCLUSIONS: Air assistance and flat-fan nozzles allow volume rates to be reduced while maintaining or improving spray quality distribution. The working parameters of hand-held sprayers must be considered to reduce environmental risk and increase the efficacy of the spraying process. © 2015 Society of Chemical Industry.