Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 60
Filtrar
1.
Vet Med Sci ; 9(6): 2676-2685, 2023 11.
Artículo en Inglés | MEDLINE | ID: mdl-37771165

RESUMEN

BACKGROUND: Since 2005, highly pathogenic avian influenza A H5N1 viruses have spread from Asia worldwide, infecting poultry, humans and wild birds. Subsequently, global interest in avian influenza (AI) surveillance increased. OBJECTIVES: Mongolia presents an opportunity to study viruses in wild birds because the country has very low densities of domestic poultry and supports large concentrations of migratory water birds. METHODS: We conducted AI surveillance in Mongolia over two time periods, 2009-2013 and 2016-2018, utilizing environmental fecal sampling. Fresh fecal samples were collected from water bird congregation sites. Hemagglutinin (HA) and neuraminidase (NA) subtypes of positive samples were identified through viral isolation or molecular assays, with pathogenicity determined by HA subtype or sequencing the HA cleavage site. RESULTS: A total of 10,222 samples were collected. Of these, 7,025 fecal samples were collected from 2009 to 2013, and 3,197 fecal samples were collected from 2016 to 2018. Testing revealed 175 (1.7%) positive samples for low-pathogenicity influenza A, including 118 samples from 2009 to 2013 (1.7%) and 57 samples from 2016 to 2018 (1.8%). HA and NA subtyping of all positives identified 11 subtypes of HA and nine subtypes of NA in 29 different combinations. Within periods, viruses were detected more frequently during the fall season than in the early summer. CONCLUSION: Mongolia's critical wild bird habitat is positioned as a crossroad of multiple migratory flyways. Our work demonstrates the feasibility of using an affordable environmental fecal sampling approach for AI surveillance and contributes to understanding the prevalence and ecology of low-pathogenicity avian influenza viruses in this important location, where birds from multiple flyways mix.


Asunto(s)
Subtipo H5N1 del Virus de la Influenza A , Virus de la Influenza A , Gripe Aviar , Humanos , Animales , Gripe Aviar/epidemiología , Mongolia/epidemiología , Virulencia , Animales Salvajes , Aves , Agua
2.
Pathogens ; 12(2)2023 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-36839475

RESUMEN

From wild dogs (Lycaon pictus) in the Serengeti to tigers (Panthera tigris altaica) in the Russian Far East, canine distemper virus (CDV) has been repeatedly identified as a threat to wild carnivores. Between 2020 and 2022, six Indian leopards (P. pardus fusca) presented to Nepali authorities with fatal neurological disease, consistent with CDV. Here, we report the findings of a serosurvey of wild felids from Nepal. A total of 48 serum samples were tested, comprising 28 Bengal tigers (P. t. tigris) and 20 Indian leopards. Neutralizing antibodies were identified in three tigers and six leopards, equating to seroprevalences of 11% (CI: 2.8-29.3%, n = 28) and 30% (CI: 12.8-54.3%, n = 20), respectively. More than one-third of seropositive animals were symptomatic, and three died within a week of being sampled. The predation of domestic dogs (Canis lupus familiaris) has been posited as a potential route of infection. A comparison of existing diet studies revealed that while leopards in Nepal frequently predate on dogs, tigers do not, potentially supporting this hypothesis. However, further work, including molecular analyses, would be needed to confirm this.

3.
PLoS One ; 18(2): e0281542, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36848365

RESUMEN

Canine distemper virus (CDV) is a global multi-host pathogen that is capable of causing considerable mortality in a range of species and is important in the field of conservation medicine. Nepal's Chitwan National Park is a protected area providing habitat for 32% of the country's mammal species including endangered carnivores such as the Bengal tiger (Panthera tigris tigris) that are susceptible to CDV. The presence of free-roaming dogs around protected areas could represent a source of infectious disease for transmission to local wildlife. A cross-sectional demographic and canine distemper virus seroprevalence study of 100 free-roaming dogs from the Chitwan National Park buffer zone and surrounding area was conducted in November 2019. The overall seroprevalence indicating past exposure to canine distemper virus was 80.0% (95% CI: 70.8-87.3). Of the host variables assessed, sex and age were positively associated with seroprevalence at the univariable level, with male dogs demonstrating lower seroprevalence than females (OR = 0.32, 95% CI: 0.11-0.91) and adult dogs demonstrating higher seroprevalence than juveniles (OR = 13.94, 95% CI: 1.37-142.29). The effect of sex was no longer significant at the multivariable level, but the direction of the effect remained the same. The effect of age remained significant after multivariable analysis (OR = 9.00, 95% CI: 1.03-192.75). No spatial associations were demonstrated in relation to the buffer zone area or boundary of Chitwan National Park. Free-roaming dog neutering and vaccination programmes can provide a useful baseline for future CDV studies in the region, and a proxy to monitor disease threats to susceptible wildlife.


Asunto(s)
Canidae , Virus del Moquillo Canino , Masculino , Femenino , Animales , Perros , Nepal/epidemiología , Estudios Transversales , Parques Recreativos , Estudios Seroepidemiológicos , Animales Salvajes
4.
SAGE Open Med Case Rep ; 10: 2050313X221117884, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35991953

RESUMEN

With the COVID-19 pandemic, vaccines have been administered all around the world probably more than ever. Even though they are considered safe, with such a huge quantity of doses used, many adverse effects including cutaneous ones were reported. We report here the case of a male adult with a history of monoclonal gammopathy of undetermined significance who presented with an extensive cutaneous eruption of indurated erythematous papules and plaques 2 days after receiving his first dose of Moderna COVID-19 vaccine (mRNA-1273 SARS-CoV-2 vaccine). Histopathology was compatible with a histiocytoid Sweet syndrome and history suggested that the vaccine contributed to the eruption.

5.
J Wildl Dis ; 57(2): 464-466, 2021 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-33822169

RESUMEN

Canine distemper virus (CDV) is recognized as a conservation threat to Amur tigers (Panthera tigris altaica) in Russia, but the risk to other subspecies remains unknown. We detected CDV neutralizing antibodies in nine of 21 wild-caught Sumatran tigers (42.9%), including one sampled on the day of capture, confirming exposure in the wild.


Asunto(s)
Anticuerpos Antivirales/sangre , Virus del Moquillo Canino , Tigres/sangre , Animales , Animales Salvajes , Anticuerpos Neutralizantes , Indonesia/epidemiología , Pruebas de Neutralización , Proyectos Piloto , Estudios Seroepidemiológicos
6.
Int J Parasitol Parasites Wildl ; 14: 150-156, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33665082

RESUMEN

The endangered Bengal tiger (Panthera tigris tigris) is a keystone species playing an essential role in ecology as well as in the social and spiritual lives of the Himalayan people. The latest estimate of the Bengal tiger population in Bhutan accounts for 103 individuals. Infectious organisms, including zoonotic parasites causing high burden in human health, have received little attention as a cause of mortality in tigers. Taeniosis/cysticercosis, caused by the cestode Taenia solium, is considered one of the major neglected tropical diseases in Southeast Asia. We present here a case of neurocysticercosis in a Bengal tiger showing advanced neurological disease outside Thimphu, the capital city of Bhutan. After palliative care, the animal died, and necropsy revealed multiple small cysts in the brain. Here we show the presence of two genetic variants of T. solium in the parasite material collected based on PCR and sequencing of the complete cox1 and cytB genes. The sequences form a discrete branch within the Asia plus Madagascar cluster of the parasite. On other hand, tests for feline morbillivirus, feline calicivirus, canine distemper virus, Nipah, rabies, Japanese encephalitis, feline leukaemia and feline immunodeficiency virus were negative. In contrast, PCR for feline herpesvirus was positive and a latex agglutination test revealed an elevated antibody titer against Toxoplasma gondii (titer 1:256). The molecular examination of taeniid eggs isolated from the tiger faeces produced sequences for which the highest homology in GenBank is between 92% and 94% with T. regis and T. hydatigena. This fatal case of T. solium neurocysticercosis, a disease previously unrecorded in tigers or other non-domestic felids, demonstrates an anthropogenically driven transmission of a deadly pathogen which could become a serious threat to the tiger population.

7.
Mol Biol Evol ; 38(6): 2366-2379, 2021 05 19.
Artículo en Inglés | MEDLINE | ID: mdl-33592092

RESUMEN

Species conservation can be improved by knowledge of evolutionary and genetic history. Tigers are among the most charismatic of endangered species and garner significant conservation attention. However, their evolutionary history and genomic variation remain poorly known, especially for Indian tigers. With 70% of the world's wild tigers living in India, such knowledge is critical. We re-sequenced 65 individual tiger genomes representing most extant subspecies with a specific focus on tigers from India. As suggested by earlier studies, we found strong genetic differentiation between the putative tiger subspecies. Despite high total genomic diversity in India, individual tigers host longer runs of homozygosity, potentially suggesting recent inbreeding or founding events, possibly due to small and fragmented protected areas. We suggest the impacts of ongoing connectivity loss on inbreeding and persistence of Indian tigers be closely monitored. Surprisingly, demographic models suggest recent divergence (within the last 20,000 years) between subspecies and strong population bottlenecks. Amur tiger genomes revealed the strongest signals of selection related to metabolic adaptation to cold, whereas Sumatran tigers show evidence of weak selection for genes involved in body size regulation. We recommend detailed investigation of local adaptation in Amur and Sumatran tigers prior to initiating genetic rescue.


Asunto(s)
Evolución Biológica , Flujo Genético , Endogamia , Selección Genética , Tigres/genética , Animales , Conservación de los Recursos Naturales , Variación Genética , Genoma , India , Filogeografía
8.
Pest Manag Sci ; 77(5): 2219-2230, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33345441

RESUMEN

BACKGROUND: Attractant-based trapping is used in the establishment of pest-free areas and areas of low pest prevalence for fruit flies (Diptera: Tephritidae). Male lures are commonly used attractants in fruit fly trapping. In this study, the effects of male lure dispensers, traps and combinations of dispensers and traps on monitoring of two fruit fly pests, Ceratitis capitata (Wiedemann) and Bactrocera dorsalis (Hendel), were investigated in South Africa. RESULTS: In the male-lure-based trapping systems evaluated, trimedlure (TML) for C. capitata and methyl eugenol (ME) for B. dorsalis, the type of dispenser affected catches for both species. Higher catches of B. dorsalis males were recorded in bucket traps baited with a dispenser containing 15 g ME compared with traps baited with dispensers containing either 4 g ME or 2 g ME. Catches of C. capitata males were higher with dispensers containing TML than those with TML plus extender (Capilure®). The type of trap used with TML also influenced catches of C. capitata with higher numbers recorded in yellow Delta trap compared with the Sensus bucket trap. CONCLUSIONS: Dispensers with higher ME loadings are more effective for monitoring of B. dorsalis. The yellow Delta trap baited with TML (without extender) would be an effective monitoring system for C. capitata. Fruit fly prevalence levels as determined by specific trapping systems should be related to their efficiency in terms of catches of the target pests. © 2020 Society of Chemical Industry.


Asunto(s)
Ceratitis capitata , Tephritidae , Animales , Control de Insectos , Masculino , Feromonas/farmacología , Sudáfrica
9.
Proc Natl Acad Sci U S A ; 117(50): 31954-31962, 2020 12 15.
Artículo en Inglés | MEDLINE | ID: mdl-33229566

RESUMEN

Canine distemper virus (CDV) has recently emerged as an extinction threat for the endangered Amur tiger (Panthera tigris altaica). CDV is vaccine-preventable, and control strategies could require vaccination of domestic dogs and/or wildlife populations. However, vaccination of endangered wildlife remains controversial, which has led to a focus on interventions in domestic dogs, often assumed to be the source of infection. Effective decision making requires an understanding of the true reservoir dynamics, which poses substantial challenges in remote areas with diverse host communities. We carried out serological, demographic, and phylogenetic studies of dog and wildlife populations in the Russian Far East to show that a number of wildlife species are more important than dogs, both in maintaining CDV and as sources of infection for tigers. Critically, therefore, because CDV circulates among multiple wildlife sources, dog vaccination alone would not be effective at protecting tigers. We show, however, that low-coverage vaccination of tigers themselves is feasible and would produce substantive reductions in extinction risks. Vaccination of endangered wildlife provides a valuable component of conservation strategies for endangered species.


Asunto(s)
Moquillo/prevención & control , Especies en Peligro de Extinción/economía , Tigres/virología , Vacunación/economía , Vacunas Virales/administración & dosificación , Animales , Animales Salvajes/virología , Toma de Decisiones en la Organización , Reservorios de Enfermedades/veterinaria , Reservorios de Enfermedades/virología , Moquillo/epidemiología , Moquillo/transmisión , Moquillo/virología , Virus del Moquillo Canino/genética , Virus del Moquillo Canino/inmunología , Perros/sangre , Perros/virología , Estudios de Factibilidad , Femenino , Masculino , Modelos Económicos , Filogenia , Estudios Seroepidemiológicos , Siberia , Tigres/sangre , Vacunación/métodos , Cobertura de Vacunación/economía , Cobertura de Vacunación/métodos , Cobertura de Vacunación/organización & administración , Vacunas Virales/economía
11.
PLoS One ; 15(8): e0237129, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32776964

RESUMEN

Outbreaks of emerging coronaviruses in the past two decades and the current pandemic of a novel coronavirus (SARS-CoV-2) that emerged in China highlight the importance of this viral family as a zoonotic public health threat. To gain a better understanding of coronavirus presence and diversity in wildlife at wildlife-human interfaces in three southern provinces in Viet Nam 2013-2014, we used consensus Polymerase Chain Reactions to detect coronavirus sequences. In comparison to previous studies, we observed high proportions of positive samples among field rats (34.0%, 239/702) destined for human consumption and insectivorous bats in guano farms (74.8%, 234/313) adjacent to human dwellings. Most notably among field rats, the odds of coronavirus RNA detection significantly increased along the supply chain from field rats sold by traders (reference group; 20.7% positivity, 39/188) by a factor of 2.2 for field rats sold in large markets (32.0%, 116/363) and 10.0 for field rats sold and served in restaurants (55.6%, 84/151). Coronaviruses were also detected in rodents on the majority of wildlife farms sampled (60.7%, 17/28). These coronaviruses were found in the Malayan porcupines (6.0%, 20/331) and bamboo rats (6.3%, 6/96) that are raised on wildlife farms for human consumption as food. We identified six known coronaviruses in bats and rodents, clustered in three Coronaviridae genera, including the Alpha-, Beta-, and Gammacoronaviruses. Our analysis also suggested either mixing of animal excreta in the environment or interspecies transmission of coronaviruses, as both bat and avian coronaviruses were detected in rodent feces on wildlife farms. The mixing of multiple coronaviruses, and their apparent amplification along the wildlife supply chain into restaurants, suggests maximal risk for end consumers and likely underpins the mechanisms of zoonotic spillover to people.


Asunto(s)
Animales Salvajes/virología , Infecciones por Coronavirus/epidemiología , Infecciones por Coronavirus/transmisión , Coronavirus/genética , Carne/virología , Zoonosis/epidemiología , Zoonosis/transmisión , Animales , Quirópteros/virología , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/virología , Reservorios de Enfermedades/virología , Heces/virología , Abastecimiento de Alimentos , Humanos , Filogenia , Reacción en Cadena de la Polimerasa , Puercoespines/virología , ARN Viral/genética , Ratas , Riesgo , Vietnam/epidemiología , Zoonosis/diagnóstico , Zoonosis/virología
12.
Arch Virol ; 165(8): 1869-1875, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32488616

RESUMEN

Coronaviruses can become zoonotic, as in the case of COVID-19, and hunting, sale, and consumption of wild animals in Southeast Asia increases the risk for such incidents. We sampled and tested rodents (851) and other mammals and found betacoronavirus RNA in 12 rodents. The sequences belong to two separate genetic clusters and are closely related to those of known rodent coronaviruses detected in the region and distantly related to those of human coronaviruses OC43 and HKU1. Considering the close human-wildlife contact with many species in and beyond the region, a better understanding of virus diversity is urgently needed for the mitigation of future risks.


Asunto(s)
Animales Salvajes/virología , Betacoronavirus/genética , Infecciones por Coronavirus/veterinaria , Pandemias/veterinaria , Neumonía Viral/veterinaria , ARN Viral/genética , Roedores/virología , Animales , Betacoronavirus/aislamiento & purificación , COVID-19 , Quirópteros/virología , Coronavirus Humano OC43/genética , Humanos , Laos/epidemiología , ARN Viral/aislamiento & purificación , SARS-CoV-2
13.
Front Vet Sci ; 7: 50, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32232059

RESUMEN

Growing evidence suggests that multiple wildlife species can be infected with peste des petits ruminants virus (PPRV), with important consequences for the potential maintenance of PPRV in communities of susceptible hosts, and the threat that PPRV may pose to the conservation of wildlife populations and resilience of ecosystems. Significant knowledge gaps in the epidemiology of PPRV across the ruminant community (wildlife and domestic), and the understanding of infection in wildlife and other atypical host species groups (e.g., camelidae, suidae, and bovinae) hinder our ability to apply necessary integrated disease control and management interventions at the wildlife-livestock interface. Similarly, knowledge gaps limit the inclusion of wildlife in the FAO/OIE Global Strategy for the Control and Eradication of PPR, and the framework of activities in the PPR Global Eradication Programme that lays the foundation for eradicating PPR through national and regional efforts. This article reports on the first international meeting on, "Controlling PPR at the livestock-wildlife interface," held in Rome, Italy, March 27-29, 2019. A large group representing national and international institutions discussed recent advances in our understanding of PPRV in wildlife, identified knowledge gaps and research priorities, and formulated recommendations. The need for a better understanding of PPRV epidemiology at the wildlife-livestock interface to support the integration of wildlife into PPR eradication efforts was highlighted by meeting participants along with the reminder that PPR eradication and wildlife conservation need not be viewed as competing priorities, but instead constitute two requisites of healthy socio-ecological systems.

14.
Transbound Emerg Dis ; 67(3): 1177-1188, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31833654

RESUMEN

Translocation of wildlife as a means of reintroducing or reinforcing threatened populations is an important conservation tool but carries health risks for the translocated animals and their progeny, as well as wildlife, domestic animals and humans in the release area. Disease risk analyses (DRA) are used to identify, prioritize and design mitigation strategies to address these threats. Here, we use a DRA undertaken for Amur leopards (Panthera pardus orientalis) to illustrate how specific methodology can optimize mitigation strategy design. A literature review identified a total of 98 infectious hazards and 28 non-infectious hazards. Separate analyses were undertaken for disease risks in leopards from hazards of source origin (captive zoo collections and the transit pathway to the Russian Far East), or of destination origin (in breeding enclosures and wider release areas); and for disease risks in other wildlife, domesticated species or humans, similarly from hazards of source or destination origin. Hazards were assessed and ranked as priority 1, priority 2, priority 3 or low priority in each of the defined scenarios. In addition, we undertook a generic assessment of stress on individual leopards. We use three examples to illustrate the process: Chlamydophila felis, canine distemper virus (CDV) and feline immunodeficiency virus (FIV). We found that many potentially expensive screening procedures could be performed prior to export of leopards, putting the onus of responsibility onto the zoo sector, for which access to diagnostic testing facilities is likely to be optimal. We discuss how our methods highlighted significant data gaps relating to pathogen prevalence in the Russian Far East and likely future unpredictability, in particular with respect to CDV. There was emphasis at all stages on record keeping, meticulous planning, design, staff training and enclosure management, which are relatively financially inexpensive. Actions to minimize stress featured at all time points in the strategy and also focussed on planning, design and management.


Asunto(s)
Infecciones por Chlamydia/veterinaria , Conservación de los Recursos Naturales/métodos , Moquillo/virología , Infecciones por Lentivirus/veterinaria , Panthera , Animales , Chlamydia , Infecciones por Chlamydia/virología , Virus del Moquillo Canino , Virus de la Inmunodeficiencia Felina , Infecciones por Lentivirus/virología , Siberia
15.
Phys Rev Lett ; 123(3): 031101, 2019 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-31386438

RESUMEN

The Laser Ranging Interferometer (LRI) instrument on the Gravity Recovery and Climate Experiment (GRACE) Follow-On mission has provided the first laser interferometric range measurements between remote spacecraft, separated by approximately 220 km. Autonomous controls that lock the laser frequency to a cavity reference and establish the 5 degrees of freedom two-way laser link between remote spacecraft succeeded on the first attempt. Active beam pointing based on differential wave front sensing compensates spacecraft attitude fluctuations. The LRI has operated continuously without breaks in phase tracking for more than 50 days, and has shown biased range measurements similar to the primary ranging instrument based on microwaves, but with much less noise at a level of 1 nm/sqrt[Hz] at Fourier frequencies above 100 mHz.

16.
PLoS Pathog ; 15(2): e1007531, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30731004

RESUMEN

Virus ecology and evolution play a central role in disease emergence. However, their relative roles will vary depending on the viruses and ecosystems involved. We combined field studies, phylogenetics and experimental infections to document with unprecedented detail the stages that precede initial outbreaks during viral emergence in nature. Using serological surveys we showed that in the absence of large-scale outbreaks, horses in Mongolia are routinely exposed to and infected by avian influenza viruses (AIVs) circulating among wild birds. Some of those AIVs are genetically related to an avian-origin virus that caused an epizootic in horses in 1989. Experimental infections showed that most AIVs replicate in the equine respiratory tract without causing lesions, explaining the absence of outbreaks of disease. Our results show that AIVs infect horses but do not spread, or they infect and spread but do not cause disease. Thus, the failure of AIVs to evolve greater transmissibility and to cause disease in horses is in this case the main barrier preventing disease emergence.


Asunto(s)
Caballos/inmunología , Gripe Aviar/genética , Animales , Animales Salvajes , Asia , Evolución Biológica , Aves , Brotes de Enfermedades , Transmisión de Enfermedad Infecciosa/veterinaria , Evolución Molecular , Caballos/genética , Humanos , Gripe Aviar/inmunología , Gripe Humana , Infecciones por Orthomyxoviridae/veterinaria , Filogenia
17.
Virus Res ; 259: 46-53, 2019 01 02.
Artículo en Inglés | MEDLINE | ID: mdl-30385363

RESUMEN

Herpesvirus infection was investigated in black bears (Ursus americanus) with neurological signs and brain lesions of nonsuppurative encephalitis of unknown cause. Visible cytopathic effects (CPE) could only be observed on days 3-5 post-infection in HrT-18G cell line inoculated with bear tissue extracts. The observed CPE in HrT-18G cells included syncytia, intranuclear inclusions, and cell detachments seen in herpesvirus infection in vitro. Herpesvirus-like particles were observed in viral culture supernatant under the electron microscope, however, capsids ranging from 60 nm to 100 nm in size were often observed in viral cultures within the first two passages of propagation. Herpesvirus infection in the bear tissues and tissue cultures were detected by PCR using degenerate primers specific to the DNA polymerase gene (DPOL) and glycoprotein B gene (gB). DNA sequencing of the amplicon revealed that the detected herpesvirus has 94-95% identity to Ursid gammaherpesvirus 1 (UrHV-1) DNA sequences of DPOL. Phylogenetic analysis of DPOL sequences indicates that black bear herpesviruses and UrHV-1 are closely related and have small distances to members of Rhadinovirus. Interestingly, black bear herpesvirus infections were also found in bears without neurological signs. The DPOL DNA sequence of black bear herpesviruses detected in neurological bears were similar to the those detected in the non-neurological bears. However, the gB DNA sequence detected from the neurological bear is different from non-neurological bear and has only 64.5%-70% identity to each other. It is possible that at least two different types of gammaherpesviruses are present in the U. americanus population or several gammaherpesviruses exist in ursine species.


Asunto(s)
Enfermedades de los Animales/virología , Gammaherpesvirinae/fisiología , Infecciones por Herpesviridae/veterinaria , Ursidae/virología , Enfermedades de los Animales/patología , Animales , Línea Celular , Efecto Citopatogénico Viral , ADN Viral , Femenino , Gammaherpesvirinae/clasificación , Gammaherpesvirinae/aislamiento & purificación , Gammaherpesvirinae/ultraestructura , Masculino , Filogenia , Análisis de Secuencia de ADN
18.
Oncoimmunology ; 7(8): e1467856, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30221071

RESUMEN

CTCL follows different courses depending on the clinical stage at the time of diagnosis. Patients with early stage Mycosis Fungoides (MF) variant of CTCL may experience an indolent course over decades, whereas patients with advanced MF and Sézary Syndrome (SS) disease at diagnosis, often succumb within 5 years. Even within early stage CTCL/MF, a minority of patients will progress to more advanced stages. We recently generated RNA sequencing data on 284 CTCL-relevant genes for 157 patients and identified differentially expressed genes across stages I-IV. In this study, we aim to validate robust molecular markers linked to disease progression and survival. We performed multiple hypothesis testing-corrected analysis of variance (ANOVA) on the expression of individual genes across all CTCL samples and early stage (≤IIA) CTCL/MF patients. We used in silico immune cell-type deconvolution from gene expression data to estimate immune cell populations. Based on the analysis of all CTCL samples, we identified TOX, FYB, and CD52 as predictors of disease progression and poor survival. Among early stage (≤IIA) CTCL/MF patients, these 3 genes, along with CCR4, were valuable to predict disease progression. We validated these 4 genes in 3 independent, external Sézary Syndrome patient cohorts with RNA-Sequencing data. In silico immune cell-type deconvolution revealed that neutrophil infiltration in early stage MF conveyed a higher risk for disease progression. Also, NK cell infiltration in late stage MF/SS correlated with improved survival. TOX, FYB, CCR4 and CD52 are robust disease progression and decreased survival biomarkers in CTCL.

19.
J Insect Sci ; 18(2)2018 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-29718494

RESUMEN

Scirtothrips aurantii Faure (Thysanoptera: Thripidae) is a major pest of citrus fruit in subtropical southern Africa. Population monitoring is an important aspect of S. aurantii control, but additional information is required on its phenology. Dispersal of mature larvae onto the soil surface from the tree canopy, and emergence of adults, were assessed using dispersal/emergence (D/E) traps in an untreated citrus orchard in South Africa. Overall, 90.7% of adult Thysanoptera emerging from soil / leaf litter beneath the citrus trees were S. aurantii, of which 35.7% were males, and 64.3% were females. Female S. aurantii having survived winter as adults oviposited on the early spring flush. This resulted in the first population peak of larvae dropping to the ground to pupate and adults emerging in spring, September to early October, as the first vegetative flush of the citrus-growing season hardened, and fruit was set as blossoming ended. Initial infestation of young fruit occurred after a build-up of larval numbers on the soft citrus flush late July to early August. The second generation larval and adult peak occurred late November to early December, whether there was new flush or not, as young fruit could support the S. aurantii population. A third peak occurred mid-December to late January, depending on year. Thus, there were three generations of S. aurantii during the period of citrus fruit susceptibility to thrips damage (September-January). After the autumn flush in April, another peak of larvae and adults occurred before the population declined to a minimum from May to July.


Asunto(s)
Distribución Animal , Thysanoptera , Animales , Citrus/fisiología , Femenino , Masculino , Dinámica Poblacional
20.
J Drugs Dermatol ; 17(3): 253-262, 2018 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-29537443

RESUMEN

BACKGROUND: Recently, experience and knowledge have been gained using effective topical treatment for onychomycosis, a difficult-to-treat infection. METHODS: This project aims to help understand and improve patient-focused quality of care for fungal nail infections. A panel of dermatologists who treat onychomycosis convened on several occasions to review and discuss recent learnings in the treatment of onychomycosis. The panel developed and conducted a survey on diagnosis, treatment and prevention, discussed the results, and provided recommendations. RESULTS: The survey was sent out digitally to the Canadian Dermatology community. Ninety-two dermatologists completed the questionnaires, which were included in the analysis. The survey respondents and panel members agreed that the diagnosis of toe onychomycosis should be confirmed with a positive microscopic examination for fungus or a positive mycological culture when oral therapy and/or topical treatment is prescribed, except when it is not clinically feasible, in which case topical therapy could be started based on clinical presentation. The panel and survey respondents also agreed that treatment is to be based on percentage of nail involvement: less than 20%=topical efinaconazole; 20%-60%=topical efinaconazole±oral terbinafine (for greater than 3 nails); greater than 60%=oral terbinafine±topical therapy. CONCLUSIONS: The current treatment paradigm for onychomycosis may have shifted from mainly oral antifungals to topical treatment, improving patient-focused quality of care.

J Drugs Dermatol. 2018;17(3):253-262.

.


Asunto(s)
Antifúngicos/administración & dosificación , Consenso , Dermatólogos/normas , Onicomicosis/diagnóstico , Onicomicosis/tratamiento farmacológico , Encuestas y Cuestionarios/normas , Administración Oral , Administración Tópica , Adulto , Dermatólogos/tendencias , Femenino , Humanos , Masculino , Resultado del Tratamiento
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...