Hematosalivarian barrier: structure, functions, study methods (review of literature).

The human body consists of various systems (blood, tissues, extracellular fluid, intracellular contents) separated by biological membranes. Physiological barriers ensure the physico-chemical composition of the internal environment remains constant and protects the body from environmental changes. The permeability of the histohematic barrier depends on the concentration of substances in the blood, the body's condition, external influences, and a number of other reasons caused by stimuli coming from the external or internal environment. Information about the state of the regulatory systems of the body has its effect on specific chemoreceptors, which leads to the emergence of local and general physiological and biochemical processes. According to their localization, they distinguish between the hematoencephalic, hemato-placental, hemato-ophthalmic, and hemato-salivary barriers. Recently, the hematosalivary barrier, through which the selective entry of substances from the blood into the oral fluid is carried out, has taken a special place in the study. Its functioning depends on the processes occurring in the body, which is carried out by selective permeability for substances that determine the composition of the main internal environment of the body - blood. Hematosalivary barrier is an important link in maintaining homeostasis, which is reflected in the metabolic parameters of oral fluid.

Molecular profile of oral fluid in new coronavirus infection.

Oral fluid is an alternative biological material that confirms correlations with blood parameters in various pathological conditions of the body. In order to find a non-invasive approach to stratification of patients with COVID-19 disease, molecular biomarkers of the oral fluid have been determined in patients with moderate coronavirus infection in comparison with clinically healthy individuals. It has been shown that proteomic, carbohydrate, macro- and microelement profiles of the oral fluid in coronavirus infection can be used for diagnostics. The features of protein metabolism were revealed: an increase in the content of total protein, urea; increased activity of enzymes aspartate aminotransferase, gamma glutamyl transpeptidase, creatine phosphokinase, alkaline phosphatase; changes in carbohydrate metabolism, which is expressed by an increase in glucose and lactate levels, an increase in lactate dehydrogenase activity, sodium, chloride, calcium, magnesium, iron content.

[Characteristics of the metabolic status of children of the first year of life with protein-energy deficiency depending on the gestational age at birth.] / Характеристика метаболического статуса детей первого года жизни с белково-энергетической недостаточностью в зависимости от гестационного возраста при рождении.

Comparative analysis of energy-plastic exchange indicators in mature and premature children of the first year of life in the development of protein-energy malnutrition (PEM) was carried out. Unidirectional changes are revealed, including an increase in creatinine, lactate and creatine phosphokinase activity levels, suggesting a n increasing muscle mass deficit against the background of glucose anaerobic oxidation activation. In preterm infants, glucose and triacylglicerine levels decrease, which reflects uncompensated insufficiency of energy substrates and, accordingly, ATP level. Multidirectional deviations in metabolism are pyruvate and ATP content: increase in full-term infants and decrease in preterm infants, that should be taken into account when monitoring condition of children with PEM. A significant decrease of pyruvic acid in preterm infants against the background of the levels of total protein, albumin, hemoglobin, and transferrin, not exceeding reference values, can obviously testify to the active use of this integral metabolite to maintain the fund of substituted amino acids. Development of this pathology in both mature and premature infants creates a pre-morbid background for iron deficiency anemia-diagnostic panel, which should be supplemented by calculation of transferrin saturation coefficient. Regardless of gestational age in childbirth during the formation of PEM, the lipid spectrum is rearranged according to atherogenic type: at normal values of total cholesterol, there is a significant increase in low and very low density lipoproteins with an increase in the atherogenicity coefficient. This singles out children with the pathology in question as a risk group for the development of the atherosclerotic process later, which justifies the recommendation to control the lipid profile in children of the first year of life.

[Blood group and human diseases (review of literature).]

AB0 blood group antigens were discovered over a century ago; however, it is still important to study their role in development of various pathological conditions. Today it is known that antigenic determinants of this blood group are present not only on erythrocyte membrane but also on other cells and tissues: platelets, gastrointestinal epithelium and salivary glands, respiratory system cells. In the last decade, a large number of studies have appeared to reveal the relationship between a specific disease and blood group type, meta-analyses have been published. Previously, the authors have studied the metabolic status, cell composition and coagulation profile of clinically healthy individuals for more than on 180,000 donations, that allowed to identify groupspecific features for each blood group. This review presents generalized data on the association of such pathological conditions as coronary heart disease, thromboembolic complications, tumors of various localizations, inflammatory and destructive oral diseases, psychiatric and some infectious diseases with the presence or absence of antigenic determinants A and B. Carriers of blood group 0 (I) are generally more resistant to diseases, with the exception of H.pylori-associated gastrointestinal diseases. Carriers of «antigenic¼ blood groups A (II), B (III), AB (IV) are more susceptible to development of infectious, cardiovascular and cancer diseases. The presented data demonstrate clinical significance of the definition of group typing not only for selection of blood and its components during transfusion and transplantation, but also for diagnostics, determination of risk group and tactics for treatment patients with different nosologies.

[Сlinicmolecular indicators of inflammatory destructive damage of the oral cavity in periodontitis in persons with various group accessories of blood.]

In order to find a connection between the alteration of oral tissues and genetic predisposition to inflammatory and destructive processes in oral media, the cytokine profile of the oral fluid of clinically healthy individuals was determined for various blood group affiliations according to the AB0 system. The group-specific features of individuals with B(III) blood group were revealed: an increase of 32,5% in the content of interleukin-6 and 63,1% in the content of interleukin-8 compared with similar data for people with 0(I), A(II), AB(IV) blood groups, which can predispose to the greatest activity of the inflammatory process in the oral cavity in individuals with antigen B. Confirmation of this fact is an increase of IgA antibodies to gliadin in the blood among patients with chronic generalized periodontitis with B(III) blood group, up to 5,00 U/ml (p<0,01), which indicates the processes of acute inflammation, and along with an increase in blood IgG antibodies to transglutaminase in comparison with a group of clinically healthy individuals, it serves as an indicator of damage to the body's connective tissue at the molecular level. When examining the dental status, pronounced clinical manifestations of chronic generalized periodontitis were found in patients with A(II) blood group, the molecular foundation of which is the highest content of IgA and IgG antibodies to transglutaminase in the oral fluid (0,35 U/ml and 0,45 U/ml), which contributes to the activation of periodontal-destroying inflammatory and inflammatory processes, obviously, with a tendency to the chronic course of the disease. The studies performed allowed us to analyze in clinically healthy individuals a predisposition to alternative processes in oral environments, using gradation by group blood affiliation.

[Coagulation test features depending on the AB0-blood groups system antigenic composition.]

The maintenance of normal blood flow through the vessels is the result of the coordinated work of the coagulation and anticoagulation systems of our body. The balance of this system depends on many factors, including endothelial, humoral, platelet ones, however, we still lack knowledge about the effect of antigenic determinants on the state of the hemostatic system. This study is devoted to assessing the effect of the presence and absence of antigens on the AB0 system, presented on erythrocyte and platelet membranes, on hemostatic parameters. The study was conducted in the Clinics of Samara State Medical University and consisted of127 clinically healthy individuals who underwent a general analysis and biochemical blood analysis, 52 people with the most stable indicators of cell composition and metabolic profile were selected for a coagulation test, including determination of the activity of coagulation factors and routine tests. A significant decrease in the activity of the VIII and VII coagulation factors was revealed, as well as an increase in the prothrombin time in patients with 0 (I) blood group compared to the "antigenic" blood groups. The presence of biological variation for indicators of external and internal coagulation paths was noted, depending on the group of blood belonging to the AB0 system. The findings suggest that there is an increased susceptibility to bleeding in patients with 0 (I) blood groups due to the absence of antigenic determinants on the cell membrane, while for "antigenic" blood groups, on the contrary, there is a susceptibility to thrombosis due to increased activity of the components of the coagulation system.

[The role of small molecules in metabolism regulation (review).]

The paper focuses on intermolecular interactions, particularly interactions between proteins and natural intermediates (small molecules). Molecules with a molecular weight of up to 1000 Da are free in cytoplasmic solution and form a pool of intermediates. Methods of computer modeling for prediction of protein-proteinaceous, protein-ligand, protein - a small molecule of interactions are presented. The program for modeling predicted biological activity in silico is Prediction of Activity Spectrum for Substances (PASS). In the Search Tool for Interacting Chemicals (STITCH) system, it is possible to identify potential protein interaction partners for small molecules. A review of the literature presents modern data on small molecules - metabolic switches, such as α-glycerophosphatedihydroxyacetone phosphate, pyruvate-lactate, oxaloacetate-malate. The molecules we study have different and multiple effects on metabolism and on intercellular interaction systems. Natural intermediates are at the intersection of metabolic pathways of metabolism of proteins, carbohydrates, lipids; they are signal molecules, participate in regulation of protein function, gene expression, enzyme activity. An increasing interest in deciphering protein-small molecule/metabolite interactions at the systemic level will lay a conceptual foundation that provides insight into complex epigenetic regulation under various environmental influences. A complete interplay, including a protein-small molecule interaction, will be crucial to eventually unraveling the complex relationships between the genotype and phenotype and to provide a deeper understanding of health and disease.

[Molecular markers of the oral mucosa damage in patients with leukemia.]

The criteria for early manifestations of mucosal lesions in patients with acute and chronic leukemia are distinguished: in the absence of complaints and changes in the index assessment of the oral cavity, a block of initial dental signs in the form of edema of the tongue and the smoothness of its papillae, pallor of the oral mucosa in patients with leukemia. In theoral fluid of patients with the initial signs of dental pathology in acute leukemia, a specific spectrum of disorders was established in the form of the lowest antibody level of immunoglobulin A class to transglutaminase in combination with a high amount of Ig G antibodies to transglutaminase - 8.73 ± 0.92 U / ml, which maximal level exceeds the reference limits by almost 4 times (38.80 U / ml) - that indicates structural changes in the connective tissues of the oral cavity. Under the influence of chemotherapy in these patients, the inversion of clinical manifestations of dental pathology was noted: stomatitis II (medium) severity is formed, bypassing I degree, accompanied by a 4-fold increase of IgA antibodies to transglutaminase (4.03 ± 0, 77 U / ml, p < 0.05) in the oral fluid of patients with acute leukemia and 2.5 times for chronic leukemias (3.24 ± 0.47 U / ml, p < 0.05) compared to pre-treatment period. In this regard, it is recommended to determine the content in the oral fluid for antibodies to transglutaminase in patients with leukemia for verification of the degree of inflammatory-destructive process and its recovery. The peculiarity of immunological shifts in stomatitis of the 1st degree of severity is the highest level of IgG antibodies to transglutaminase (9.98 ± 1.50 U / ml) in the oral fluid, and at II-III degree the molecular manifestations of damage are smoother, which indicates the depression of immune processes.

[Saliva diagnostic in the research about human immune adaptation to study stress and to differrent water drinking behavior.]

85 healthy young people were participates of a randomized placebo controlled cross-over fashion. This study tested associations between different water drinking behavior, the condition of oral immune protection and stress factors over 3 months. We examined saliva IL-1, IL-4, IL-6, TNF-α, γ-IFN, α-amylase and compared them with stress-associated psychophysiological data. As a result of our study we made a saliva pretreatment plan for cytokines and amylase assays, also we tried to understand the strategy of mechanism associations between different water drinking behavior, the condition of oral immune protection and stress factors.

[The effect of hyperglycemia on indices of biochemical blood analysis in vitro.]

The purpose of study was to detect possible deviations between the results of biochemical analysis of blood and true values in conditions of hyperglycemia in vitro and dependences of the given alterations from group belonging of analyzed blood. The biochemical analysis was applied to blood samples of patients with various blood groups according system AB0 in conditions of normo- and hyperglycemia in vitro. The criterion for choice was established the content of main biochemical indices of blood (total protein, total bilirubin, glucose, total cholesterol, urea, creatinine, activity of hylaninamitotransferase (ALT), aspartataminotransferase (AST), lactatedehydrogenase (LDG)) within the reference limits. The hyperglycemia was developed in vitro using 5% glucose solution and corresponded to 20,49±0,05 and 46,37±0,63 mmol/l. The content of total protein and total cholesterol turned out to be the most stable indices. The effect of increased level of glucose to following analytes was established: total bilirubin (+10,5%), ALT activity (±13,4%), AST activity (+11,1%) and creatinine (+51,4%). The alterations of indices up to level of ±10% and higher are clinically significant and have to be taking into account by physicians of clinical laboratory diagnostic and clinical physicians during interpretations of the results of biochemical analysis of blood for evaluation of true picture of condition of patient. The AB0-group specific characteristics of biochemical analysis of blood are established in conditions of hyperglycemia. The hyperglycemia is an interfering factor during implementation of laboratory analysis that is especially actual at interpretations of results of biochemical analysis of blood in patients of reanimation department and individuals suffering of persistent and severe diabetes mellitus with its complication with nephropathy. Besides, the established group-specific characteristics of hyperglycemia effecting the results of biochemical analysis of blood can be accounted in development of standards and guidelines of personified medicine.

[Evaluation of vitamin status of workers of oil-processing enterprises (Samara Region) according to the data on vitamin consumption and blood serum level].

Vitamin status of 77 employees of oil refineries (39 men and 38 women aged 22 to 62 years, body mass index - 25.0±4.7 kg/m2) has been assessed in autumn. The sufficiency with vitamins A, E, D, B2, B12, folate and beta-carotene was evaluated by determination in blood plasma, daily intake of vitamins B1, B2, niacin, C, A and betacarotene was calculated by frequency of food consumption during previous month. All surveyed were sufficiently supplied with vitamins A, B12 and folate (reduced level was detected in 0-2.6%). The content of tocopherols referred to triglycerides was reduced in 33% of the workers, in men it was 2 fold more likely (p<0.05) than in women (43.6 vs 21.1%). Vitamin D insufficiency took place in 23.4% of individuals, vitamin B2 and beta-carotene - in 44.2%. A reduced blood plasma content of one vitamin was detected in 39.0% of the surveyed, while two vitamins - in 24.7% persons, 2.2 fold more often (p<0.05) in men than in women. Multivitamin insufficiency was detected in 10.4% of workers. Reduced blood plasma level of at least one vitamin-antioxidant was detected in 46.8% (in 61.5% of men and 31.6% of women, p<0.05). 26.0% of surveyed were provided with all 6 vitamins and beta-carotene. Reduced relative to RDA daily intake of antioxidant vitamins (ascorbic acid and beta-carotene) was observed in 64.9 and 100% of individuals, B group vitamins (niacin, B2 and B1) - in 48.1-64.9%, vitamin A - in 42.9%.

[Assessment of vitamin status of the workers of Samara thermal power plant according to data on vitamin intake and their levels in blood].

Sufficiency of 58 employees of a thermal power plant (47 men and 11 women aged 21 to 64 years, body mass index - 27.0±5.8 kg/m2), with vitamins A, E, D, B6, B12, folic acid and carotenoids (content in the blood plasma) and vitamin C (urinary excretion) was conducted in winter (February 2015). All surveyed workers were sufficiently supplied with vitamins A, B6, B12 and folic acid (a reduced level was detected in 0-9%). Insufficient supplying with vitamin E had 19% of the workers (and only men), vitamin C - 44%, vitamin D - 61%, carotenoids - 93%. Only 5% persons were sufficiently provided with all 6 vitamins. A combined deficiency of two vitamins had 38% of them, three - 22%, four - 16%. Simultaneously reduced plasma level of two antioxidants was observed in 36% of subjects, three - in 12%. Significant positive correlation (p <0.05) was detected between plasma levels: total cholesterol and retinol and ß-carotene; tocopherol and total cholesterol, triglycerides, low density lipoprotein cholesterol; between the concentration of vitamin D, and high density lipoproteins; levels of retinol and tocopherol and ß-carotene. In parallel intake of vitamins C, A, B1, B2 and niacin has been assessed by calculation of the frequency of food consumption during the previous month. Reduced relatively recommended daily intake of vitamins A, C has been found in approximately half of the surveyed, niacin, vitamin B1 and B2 - in 70-80%. Comparison of the data on the availability of vitamins C and A, obtained by calculation of vitamin intake and biochemical methods gave identical results in 55 and 60% of cases, correspondingly. The lack of vitamins has been detected more frequently by assessing dietary intake.

Changes in myocardial metabolism induced by ethanol administration.

The utilization of ethyl alcohol in the myocardium and the reactions connected with its metabolism were investigated in rabbits which were given over a period of two months a 30% ethanol solution per os in a dosage of 1 g/kg body weight, and in a control group. Alcohol intoxication led to a reduction of the reserve of oxidized nicotinamide coenzymes, to an intensification of glycolytic processes, to slowed down utilization of substrates in the citric acid cycle and to an accumulation of less-oxidized metabolism products (dihydroxyacetone phosphate, lactate, pyruvate, malate, oxaloacetate, 2-oxoglutarate). A change was also observed in the myocardial microstructure. Metabolic changes are evidently one of the links of the pathogenetic mechanism of alcohol intoxication, which cause disorders in cardiac activity diagnosed as alcoholic myocardial dystrophy.

Biochemical background of atherosclerotic heart lesion in an experiment.

The authors investigated enzymic systems and corresponding substrate cycles that transport hydrogen across the mitochondrial membrane in a myocardium with experimental cholesterol-induced atherosclerosis. Sensitive spectrophotometric methods were used for assessing the activities of cytoplasmic and mitochondrial enzymes: lactate and alpha-glycerphosphate dehydrogenases, and of characteristic mitochondrial enzymes: glutamate and beta-hydroxybutyrate dehydrogenases. Specific enzymological methods were used in determining the concentrations of lactic, pyruvic, glycerophosphoric, dihydroacetonephosphoric, malic, oxaloacetic, glutamic, alpha-ketoglutaric, acetoacetic, and beta-hydroxybutyric acids. The cytoplasmic NAD/NADH quotient was calculated. The investigators found a reduction of enzymic activities in the "shuttle" transport system studied, marked deviations from normal levels of their substrates, and a reduction of the NAD/NADH quotient by the factor 0.56. All these phenomena represent a biochemical background of a complex of symptoms characterizing severe myocardial lesion in experimental atherosclerosis.