Polyhydroxyalkanoates synthesis using acidogenic fermentative effluents.

Polyhydroxyalkanoates (PHA) are natural polyesters synthesized by microbes which consume excess amount of carbon and less amount of nutrients. It is biodegradable in nature, and it synthesized from renewable resources. It is considered as a future polymer, which act as an attractive replacement to petrochemical based polymers. The main hindrance to the commercial application of PHA is the high manufacturing cost. This article provides an overview of different cost-effective substrates, their characteristics and composition, major strains involved in economical production of PHA and biosynthetic pathways leading to accumulation of PHA. This review also covers the operational parameters, various fermentative modes including batch, fed-batch, repeated fed-batch and continuous fed-batch systems, along with advanced feeding strategies such as single pulse carbon feeding, feed forward control, intermittent carbon feeding, feast famine conditions to observe their effects for improving PHA synthesis and associated challenges. In addition, it also presents the economic analysis and future perspectives for the commercialization of PHA production process thereby making the process sustainable and lucrative with the possibility of commercial biomanufacturing.

Integrated biorefinery routes of biohydrogen: Possible utilization of acidogenic fermentative effluent.

Biohydrogen production and integration possibilities are vital towards hydrogen economy and sustainability of the environment. Acidogenic fermentation is acquiring great interest and it is one of the prime pathways to produce biohydrogen and short chain carboxylic acids. In addition to hydrogen recovery, simultaneously nearly 60 percent of the organics may get converted to ethanol, 1,3propanediol and organic acids. Besides, these organics (fermentative effluents) can be used indirectly as a raw material for the generation of value- added products such as biolipid, polyhydroxyalkanoates, excess hydrogen, methane and electrical energy recovery. In this regard, this review has been assessed as a valuable biorefinery for biofuel and value- added products recovery. The biorefinery can be used to minimize entire cost of the approach by obtaining extra profits.