Impact of capillary and sarcolemmal proximity on mitochondrial structure and energetic function in skeletal muscle.

Mitochondria within skeletal muscle cells are located either between the muscle contractile apparatus (interfibrillar mitochondria, IFM) or beneath the cell membrane (subsarcolemmal mitochondria, SSM), with several structural and functional differences reported between IFM and SSM. However, recent 3D imaging studies demonstrate that mitochondria are particularly concentrated in the proximity of capillaries embedded in sarcolemmal grooves rather than in proximity to the sarcolemma itself (paravascular mitochondria, PVM). To evaluate the impact of capillary vs. sarcolemmal proximity, we compared the structure and function of skeletal muscle mitochondria located either lateral to embedded capillaries (PVM), adjacent to the sarcolemma but not in PVM pools (SSM) or interspersed between sarcomeres (IFM). Mitochondrial morphology and interactions were assessed by 3D electron microscopy coupled with machine learning segmentation, whereas mitochondrial energy conversion was assessed by two-photon microscopy of mitochondrial membrane potential, content, calcium, NADH redox and flux in live, intact cells. Structurally, although PVM and SSM were similarly larger than IFM, PVM were larger, rounder and had more physical connections to neighbouring mitochondria compared to both IFM and SSM. Functionally, PVM had similar or greater basal NADH flux compared to SSM and IFM, respectively, despite a more oxidized NADH pool and a greater membrane potential, signifying a greater activation of the electron transport chain in PVM. Together, these data indicate that proximity to capillaries has a greater impact on resting mitochondrial energy conversion and distribution in skeletal muscle than the sarcolemma alone. KEY POINTS: Capillaries have a greater impact on mitochondrial energy conversion in skeletal muscle than the sarcolemma. Paravascular mitochondria are larger, and the outer mitochondrial membrane is more connected with neighbouring mitochondria. Interfibrillar mitochondria are longer and have greater contact sites with other organelles (i.e. sarcoplasmic reticulum and lipid droplets). Paravascular mitochondria have greater activation of oxidative phosphorylation than interfibrillar mitochondria at rest, although this is not regulated by calcium.

Usage and acceptability of data normalization in baseball pitching.

In baseball pitching biomechanics, kinetic values are commonly ratio 'normalised' by dividing by mass or mass*height to allow for comparison between athletes of different sizes. However, creating a normalised ratio variable should meet certain statistical assumptions. Our purpose was to determine if elbow valgus torque predicted by pitching velocity is influenced by normalisation using regression model comparison with and without normalised torque values. Motion capture data for youth to professional pitchers (n = 1988) were retrospectively analysed. Normalisation assumptions were tested by comparing linear regression models to analogous models with an intercept fixed at zero and by examining remaining correlations between the confounding variable and new, normalised variable. Both mass (p < 0.001) and mass*height (p < 0.001) normalisation did not remove their respective relationship with torque. After accounting for mass or mass and height, velocity predicted 10% of variance in elbow valgus torque, whereas velocity predicted 59% of mass normalised torque and 45% of mass*height normalised torque. Ratio normalisation does not fully account for anthropometric variables that differ across pitchers and leads to different conclusions in the magnitude of velocity's predictive effect on elbow valgus torque. Therefore, we recommend using regression model comparison to account for anthropometric variables in baseball pitching kinetic data.

Length-Tension Differences Between Concentric and Eccentric Shoulder Rotation Strength.

ABSTRACT: Giordano, KA, Cassidy, MM, and Oliver, GD. Length-tension differences between concentric and eccentric shoulder rotation strength. J Strength Cond Res 38(2): 253-258, 2024-Eccentric contractions generally produce more force than concentric contractions. However, if length-tension relationships affect both contractions equally remains unknown. Therefore, our purpose was to compare concentric versus eccentric shoulder external and internal rotation strength across a continuous 90° arc. Fifty-two physically active individuals performed isokinetic concentric and eccentric shoulder external rotation and internal rotation through a 90° arc (forearm horizontal to forearm vertical) with the shoulder elevated in both the frontal and scapular planes. Statistical parametric mapping analysis compared concentric and eccentric trials within subjects. Frontal plane eccentric external rotation torque was greater than concentric 30°-90° (p < 0.01) external rotation, and concentric external rotation torque was greater 5°-15° external rotation (p = 0.01). Frontal plane, eccentric internal rotation torque was greater than concentric 15°-55° external rotation (p < 0.01), and concentric torque was greater at forearm horizontal (p = 0.05) and 70°-90° external rotation (p < 0.01). Scapular plane eccentric external rotation torque was greater than concentric 30°-90° external rotation (p < 0.01) and concentric external rotation torque was greater 5°-20° external rotation (p < 0.01). Scapular plane eccentric internal rotation torque was greater than concentric 15°-60° external rotation (p < 0.01), and concentric torque was greater at forearm horizontal (p = 0.05) and 78°-90° external rotation (p = 0.02). Coaches, clinicians, and researchers should interpret data from studies reporting isokinetic data with the understanding that isokinetic peak strength values are not comparing the same muscle length and are not an appropriate measure for all muscle lengths. Furthermore, shoulder stability is affected through decreased eccentric force production at end ranges of shoulder rotation.

Ground reaction forces in baseball pitching: temporal associations with pitch velocity among high-velocity pitchers.

How baseball pitchers interact with the ground is an important aspect of pitching technique and performance. Previous studies on ground reaction forces in baseball pitching have largely been limited to pitchers at the youth or adolescent level, with only a few studies examining higher velocity pitchers. Additionally, previous studies have limited their analyses to only peak kinetic values, neglecting any temporal importance of when these peak values occur. Therefore, our purpose was to provide normative ground reaction force values and examine the associations between pitch velocity and ground reaction forces in high-velocity pitchers. We retrospectively extracted pitch velocities as well as rear and lead leg ground reaction force data from internal databases for 105 high-velocity pitchers. We analysed the associations between the full ground reaction force time series and pitch velocity using statistical parametric mapping regression. Regression analysis revealed pitch velocity significantly predicted lead leg braking ground reaction force from approximately 27% to 35% of the period between front foot contact and ball release. These data reinforce the importance of effective braking forces for achieving maximal pitch velocities. Additionally, our observed peak ground reaction force values were considerably higher than those previously reported.

Comparison of Trunk and Pelvic Kinematics in Youth Baseball Pitchers With and Without Upper Extremity Pain: A Cross-Sectional Study.

Background: Motion of the pelvis and trunk during baseball pitching is associated with increased upper extremity (UE) kinetics. Increased kinetics on the UE may lead to throwing-arm pain in youth pitchers. Limited biomechanical comparisons have been conducted on youth pitchers with and without throwing-arm pain to identify mechanical risk factors associated with pain. Purpose: To examine trunk and pelvic kinematics in youth baseball pitchers with and without UE pain. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A total of 26 male youth baseball pitchers (mean age, 12.7 ± 1.5 years; mean height, 162.2 ± 12.9 cm; mean weight, 52.6 ± 13.1 kg) were recruited to participate. An electromagnetic tracking system was used to obtain kinematic data during the fastball pitch. Data from a health history questionnaire was examined. Participants who answered "yes" to experiencing pain and who selected a region on their UE as the pain location were placed into the UE pain group. Participants who responded "no" to experiencing pain were placed into the pain-free group. We compared between-group differences in trunk rotation, flexion, and lateral flexion; pelvic rotation, anteroposterior tilt, and lateral tilt; and hip-shoulder separation from peak knee height to ball release of the baseball pitch using 1-dimensional statistical parametric mapping with an alpha level set at .05. Results: No statistically significant differences were observed between the UE pain and pain-free groups in the 7 trunk and pelvic kinematics analyzed from peak knee height to ball release (P > .05). Conclusion: Trunk and pelvic kinematics during the pitching motion did not differ between pain and pain-free groups of youth baseball pitchers.

Altered Cervical Spine Position Results in Decreased Shoulder Rotation Strength.

BACKGROUND: Strength testing of shoulder rotation is commonly used in clinical examinations of the shoulder. People prone to shoulder injury, such as overhead athletes and manual trade workers, place their shoulders under tremendous amounts of stress when the cervical spine is in nonneutral positions. If these nonneutral cervical spine positions result in decreased shoulder strength, it may help explain the etiology of the high prevalence of shoulder injuries in these populations. Given standard clinical strength assessments are performed with a neutral cervical spine, an investigation into the effects of cervical spine rotation is warranted. QUESTIONS/PURPOSES: We sought to compare isokinetic shoulder rotation strength while in a neutral position with rotated cervical spine positions, specifically (1) with the cervical spine rotated contralaterally with the shoulder elevated in the frontal plane and (2) with the cervical spine rotated ipsilaterally and the shoulder elevated in the scapular plane. METHODS: A convenience sample of 52 individuals (height 170 ± 10 cm; weight 73 ± 18 kg, age 21 ± 2 years; 18 males, 34 females), without shoulder or cervical spine pathology participated in this study. Participants were screened for eligibility via questionnaire. Concentric shoulder internal and external rotation torque was measured through a 90° arc on an isokinetic dynamometer with the shoulder elevated 90° in the frontal plane, and again 45° anterior to the frontal plane (scapular plane). Two repetitions were performed in a single testing session with the participant's cervical spine in neutral in both planes, maximally rotated contralaterally in the frontal plane, and maximally rotated ipsilaterally with the shoulder in the scapular plane; the second repetition was used for analysis. The testing order was randomized. Data were imported into a platform for statistical parametric mapping analysis (a technique that allows data from the entire arc of motion to be compared with data from another arc to identify differences in the wave form) to compare strength between positions throughout 90° arc of motion. RESULTS: Rotating the cervical spine contralaterally with the shoulder in the frontal plane resulted in a decrease in external (2.24 Nm or 12% average difference; p < 0.001) and internal (2.22 Nm or a 6% average difference; p = 0.02) rotation strength with the forearm within 15° and 20° of the vertical position. Rotating the cervical spine ipsilaterally with the shoulder in the scapular plane resulted in a decrease in external rotation strength (1.27 Nm or a 6% average difference; p < 0.001) throughout nearly all the motion, with peaks approximately 20° and 60° from the horizontal position, and internal rotation strength (1.78 Nm or 5% average difference; p < 0.001) the last 60° towards the horizontal position. CONCLUSION: Patient populations who require strenuous use of their shoulders in altered cervical spine positions may be at increased risk for injury from decreased shoulder rotator strength. CLINICAL RELEVANCE: Clinicians should assess shoulder strength in the position the patient requires to use their shoulder because cervical spine position may cause weakness that would be missed in standard testing positions.

Energy generation, absorption, and transfer at the shoulder and elbow in youth baseball pitchers.

Performance during the baseball pitch is dependent on the flow of mechanical energy through the kinetic chain. Little is known about energy flow during the pitching motion and it is not known whether patterns of energy flow are related to pitching performance and injury risk. Therefore, the purpose of this study was to quantify energy generation, absorption, and transfer across the shoulder and elbow during the baseball pitch and explore the associations between these energetic measures, pitch speed, and traditional measures of upper extremity joint loading. The kinematics of 40 youth baseball pitchers were measured in a controlled laboratory setting. Energy flow between the thorax, humerus, and forearm was calculated using a segmental power analysis. Regression analyses revealed that pitch speed was best predicted by arm cocking phase shoulder energy transfer to the humerus and peak elbow valgus torque was best predicted by arm acceleration-phase elbow energy transfer to the forearm. Additionally, energy transfer across the shoulder and elbow generally exhibited the strongest correlations to pitch speed and upper extremity joint loads. These data reinforce the importance of energy transfer through the kinetic chain for producing high pitch speeds and provide descriptive data for energy flow during baseball pitching not previously found in the literature.

Lower Extremity Pain and Pitching Kinematics and Kinetics in Collegiate Softball Pitchers.

The primary aims of the study were (1) to examine kinematics and kinetics of those pitching with and without lower extremity pain in collegiate softball pitchers, and (2) to determine if there was an association between the lower extremity pain and lower extremity kinematics, trunk kinematics, and shoulder kinetics in collegiate softball pitchers. Thirty-seven NCAA Division I female collegiate softball pitchers (19.8±1.3 yrs,173.7±7.7 cm, 79.0±12.4 kg) participated. Participants were divided into two groups, those who were currently experiencing lower extremity pain and those who were not. Participants threw three rise ball pitches. Kinematic data were collected at 100 Hz using an electromagnetic tracking system. Mann-Whitney U tests revealed no significant kinematic or kinetic differences between pitchers with and without lower extremity pain. Additionally, there were no significant correlations between pain and recorded kinematic and kinetic variables. Considering there were no biomechanical differences observed between pitchers, coaches and athletic trainers should take caution with athlete assessment since athletes may not display altered biomechanics. Further examination into the duration and degree of pain is needed in an attempt to fully understand the implication of pain and pitching mechanics.