Rhd and C/cE/e genotyping in Slovenian population.

The Rhesus (Rh) blood group system is, after ABO, clinically most important. Alloantibodies directed against Rh antigens are the major cause of a haemolytic disease of newborn (HDN) and of transfusion reactions. In search for novel methods for Rh genotyping we started to compare Rh genotypes identified from different tissues and Rh phenotypes. Genotypes determined from blood samples with PCR based RhD, C/c and E/e genotyping methods were compared with serologically identified phenotypes (N=32). With two exceptions the results of phenotyping and genotyping were in concordance. Two Rh serotypes from a Slovenian family that were unexpected according to the Mendelian laws were characterised genotypically. The two family members were suspected to have a chromosomal deletion on RH gene locus.

[Antibodies against HBs antigens as identified using the radioimmunologic method]. / Antitjela protiv HBs antigena otkrivena radioimunom metodom.

Antibodies to hepatitis Bs antigen identified only by radioimmunoassay, which is 8000 times more sensitive than counterelectrophoresis, were found in blood donors in 17%, in the Haemodialysis centre in 44%, in the patients of this centre in 47%. HBsAg and the antibodies have been tested in different series of albumin and gammaglobulin. Immunoglobulin anti HBs was prepared by the Blood transfusion centre in Ljubljana, and the Imunoloski zavod in Zagreb, and the problem of prevention, and production is discussed. Antigen-antibody complexes were prepared in vitro, and the results of the respective tests described.

[Significance of radioimmune method for detection of HB antigen in blood donors]. / Znacaj radioimune metode za testiranje HB antigena kod davalaca krvi

The evaluation of frequency of HBag in 923 blood donors is given: the counterelectrophoresis demonstrates HBag positive cases in 1, 2% the radioimmunoassay (Ausria-125, Abbott) in 1, 5%. The haemagglutination (Hepanosticon, Organon) in 7 cases, which were negative with counterelectrophoresis, and positive with the radioimmunoassay, discovers HBAg in 3 cases. RIA method is more sensitive in the control of HBAg positive donors after two to three years, and in detecting of immuno complexes prepared in vitro. The titre of the antigen is 1000 times higher when made with RIA method, and up to 60 times higher when made with the method of haemagglutination than with the counterelectrophoresis. In 4 cases of hepatitis after transfusion of HBAg negative blood, determined with counterelectrophoresis, have been involved bottles, which were positive only with RIA Method. Austria II-125 (Abbott) method was comparatively tested with counterelectrophoresis on different groups--446 persons.