Inland navigation and land use interact to impact European freshwater biodiversity.

Inland navigation in Europe is proposed to increase in the coming years, being promoted as a low-carbon form of transport. However, we currently lack knowledge on how this would impact biodiversity at large scales and interact with existing stressors. Here we addressed this knowledge gap by analysing fish and macroinvertebrate community time series across large European rivers comprising 19,592 observations from 4,049 sampling sites spanning the past 32 years. We found ship traffic to be associated with biodiversity declines, that is, loss of fish and macroinvertebrate taxonomic richness, diversity and trait richness. Ship traffic was also associated with increases in taxonomic evenness, which, in concert with richness decreases, was attributed to losses in rare taxa. Ship traffic was especially harmful for benthic taxa and those preferring slow flows. These effects often depended on local land use and riparian degradation. In fish, negative impacts of shipping were highest in urban and agricultural landscapes. Regarding navigation infrastructure, the negative impact of channelization on macroinvertebrates was evident only when riparian degradation was also high. Our results demonstrate the risk of increasing inland navigation on freshwater biodiversity. Integrative waterway management accounting for riparian habitats and landscape characteristics could help to mitigate these impacts.

Introduction of Native Submerged Macrophytes to Restore Biodiversity in Streams.

Streams are biodiversity hotspots that provide numerous ecosystem services. Safeguarding this biodiversity is crucial to uphold sustainable ecosystem functioning and to ensure the continuation of these ecosystem services in the future. However, in recent decades, streams have witnessed a disproportionate decline in biodiversity compared to other ecosystems, and are currently considered among the most threatened ecosystems worldwide. This is the result of the combined effect of a multitude of stressors. For freshwater systems in general, these have been classified into five main pressures: water pollution, overexploitation, habitat degradation and destruction, alien invasive species, and hydromorphological pressures. On top of these direct stressors, the effects of global processes like environmental and climate change must be considered. The intricate and interconnected nature of various stressors affecting streams has made it challenging to formulate effective policies and management strategies. As a result, restoration efforts have not always been successful in creating a large-scale shift towards a better ecological status. In order to achieve an improved status in these systems, situation-specific management strategies tailored to specific stressor combinations may be needed. In this paper, we examine the potential of introducing native submerged macrophyte species to advance the restoration of stream ecosystems. Through successful introductions, we anticipate positive ecological outcomes, including enhanced water quality and increased biodiversity. This research is significant, as the potential success in restoring stream biodiversity not only represents progress in ecological understanding but also offers valuable insights for future restoration and management strategies for these vital ecosystems.

Field-based molecular detection of Batrachochytrium dendrobatidis in critically endangered Atelopus toads and aquatic habitats in Ecuador.

Batrachochytrium dendrobatidis (Bd) is a lethal fungal species that parasitizes vertebrates and is associated with the worldwide decline of amphibian populations. The development of sensitive, rapid detection methods, particularly DNA-based techniques, is critical for effective management strategies. This study evaluates the efficacy of DNA extraction and a portable PCR device in a mountable field laboratory setup for detecting Bd near the habitats of three critically endangered Atelopus toad species in Ecuador. We collected skin swabs from Atelopus balios, A. nanay, and A. bomolochos, and environmental DNA (eDNA) samples from streams in Andean and coastal regions of Ecuador. For eDNA, a comparison was made with duplicates of the samples that were processed in the field and in a standard university laboratory. Our findings revealed Bd detection in eDNA and swabs from 6 of 12 water samples and 10 of 12 amphibian swab samples. The eDNA results obtained in the field laboratory were concordant with those obtained under campus laboratory conditions. These findings highlight the potential of field DNA-based monitoring techniques for detecting Bd in amphibian populations and their aquatic habitats, particularly in remote areas. Furthermore, this research aligns with the National Action Plan for the Conservation of Ecuadorian Amphibians and contributes to the global effort to control this invasive and deadly fungus.

Multi-decadal improvements in the ecological quality of European rivers are not consistently reflected in biodiversity metrics.

Humans impact terrestrial, marine and freshwater ecosystems, yet many broad-scale studies have found no systematic, negative biodiversity changes (for example, decreasing abundance or taxon richness). Here we show that mixed biodiversity responses may arise because community metrics show variable responses to anthropogenic impacts across broad spatial scales. We first quantified temporal trends in anthropogenic impacts for 1,365 riverine invertebrate communities from 23 European countries, based on similarity to least-impacted reference communities. Reference comparisons provide necessary, but often missing, baselines for evaluating whether communities are negatively impacted or have improved (less or more similar, respectively). We then determined whether changing impacts were consistently reflected in metrics of community abundance, taxon richness, evenness and composition. Invertebrate communities improved, that is, became more similar to reference conditions, from 1992 until the 2010s, after which improvements plateaued. Improvements were generally reflected by higher taxon richness, providing evidence that certain community metrics can broadly indicate anthropogenic impacts. However, richness responses were highly variable among sites, and we found no consistent responses in community abundance, evenness or composition. These findings suggest that, without sufficient data and careful metric selection, many common community metrics cannot reliably reflect anthropogenic impacts, helping explain the prevalence of mixed biodiversity trends.

Finding navigation cues near fishways.

Many fish species depend on migration for various parts of their life cycle. Well-known examples include diadromous fish such as salmon and eels that need both fresh water and salt water to complete their life cycle. Migration also occurs within species that depend only on fresh water. In recent decades, anthropogenic pressures on freshwater systems have increased greatly, and have resulted, among other effects, in drastic habitat fragmentation. Fishways have been developed to mitigate the resulting habitat fragmentation, but these are not always effective. To improve fishway efficiency, the variety of navigation cues used by fish must be better understood: fish use a multitude of sensory inputs ranging from flow variables to olfactory cues. The reaction of a fish is highly dependent on the intensity of the cue, the fish species involved, and individual traits. Recently developed monitoring technologies allow us to gain insights into different combinations of environmental and physiological conditions. By combining fish behavioural models with environmental models, interactions among these components can be investigated. Several methods can be used to analyse fish migration, with state-space models, hidden Markov models, and individual-based models potentially being the most relevant since they can use individual data and can tie them to explicit spatial locations within the considered system. The aim of this review is to analyse the navigational cues used by fish and the models that can be applied to gather knowledge on these processes. Such knowledge could greatly improve the design and operation of fishways for a wider range of fish species and conditions.

Modeling greenhouse gas emissions from riverine systems: A review.

Despite the recognized importance of flowing waters in global greenhouse gas (GHG) budgets, riverine GHG models remain oversimplified, consequently restraining the development of effective prediction for riverine GHG emissions feedbacks. Here we elucidate the state of the art of riverine GHG models by investigating 148 models from 122 papers published from 2010 to 2021. Our findings indicate that riverine GHG models have been mostly data-driven models (83%), while mechanistic and hybrid models were uncommonly applied (12% and 5%, respectively). Overall, riverine GHG models were mainly used to explain relationships between GHG emissions and biochemical factors, while the role of hydrological, geomorphic, land use and cover factors remains missing. The development of complex and advanced models has been limited by data scarcity issues; hence, efforts should focus on developing affordable automatic monitoring methods to improve data quality and quantity. For future research, we request for basin-scale studies explaining river and land-surface interactions for which hybrid models are recommended given their flexibility. Such a holistic understanding of GHG dynamics would facilitate scaling-up efforts, thereby reducing uncertainties in global GHG estimates. Lastly, we propose an application framework for model selection based on three main criteria, including model purpose, model scale and the spatiotemporal characteristics of GHG data, by which optimal models can be applied in various study conditions.

Taxonomic and Feeding Trait-Based Analysis of Macroinvertebrates in the Antisana River Basin (Ecuadorian Andean Region).

High-elevation tropical streams are under increasing threat from human activities and climate change. Specifically, Ecuadorian Andean streams require priority actions such as bioassessment (e.g., biodiversity and functional ecology of macroinvertebrates) in order to generate adequate environmental management policies. Therefore, we investigated the distribution and composition of the macroinvertebrate taxa and their functional feeding groups in relation to the environmental variables in the Antisana river basin (Andean-Ecuadorian Region). We sampled macroinvertebrates from 15 locations to assess ecological conditions (ECs), expressed as the Biological Monitoring Working Party Colombia (BMWP-Col) classes, the Andean Biotic Index (ABI) and the Andean-Amazon Biotic Index (AAMBI). Results indicate that dissolved oxygen saturation, elevation, nutrient concentration and conductivity contributed significantly to the composition of the taxa and functional feeding groups (FFGs). Taxa diversity and FFGs were more abundant in the best EC sites. Shredders (SH) were, overall, dominant and abundant at sites with medium-high ECs. Scrapers constituted the second most prevalent assemblage, exerting dominance at moderate ecological conditions (high altitude and high oxygen saturation). Collector-gathers (CGs) are less sensitive to contamination than the previous two groups but were equally abundant at medium-high EC sites. Collector-filterers (CFs) and parasites (PAs) were less abundant, although the presence of the former was slightly related to better environmental conditions. Predators (PRs) were almost absent throughout the study, but they were collected from poor EC sites. CGs, PAs and PRs showed more tolerance to the presence of human disturbances (e.g., hydraulic constructions or slope erosion). The BMWP-Col index seems to be the best fit for this ecosystem, showing a significant difference in FFG between the index classes, compared to the other indices evaluated. The results of this investigation may be regarded as a fundamental starting point and used in future bioassessment work in other similar ecosystems, particularly high-altitude tropical Ecuadorian streams.

Impact of salinity gradient, water pollution and land use types on greenhouse gas emissions from an urbanized estuary.

Estuaries have been recognized as one of the major sources of greenhouse gases (GHGs) in aquatic systems; yet we still lack insights into the impact of both anthropogenic and natural factors on the dynamics of GHG emissions. Here, we assessed the spatiotemporal dynamics and underlying drivers of the GHG emissions from the Scheldt Estuary with a focus on the effects of salinity gradient, water pollution, and land use types, together with their interaction. Overall, we found a negative impact of salinity on carbon dioxide (CO2) and nitrous oxide (N2O) emissions which can be due to the decrease of both salinity and water quality when moving upstream. Stronger impact of water pollution on the GHG emissions was found at the freshwater sites upstream compared to saline sites downstream. In particular, when water quality of the sites reduced from good, mainly located in the mouth and surrounded by arable sites, to polluted, mainly located in the upstream and surrounded by urban sites, CO2 emissions from the sites doubled while N2O emissions tripled. Similarly, the effects of water pollution on methane (CH4) emissions became much stronger in the freshwater sites compared to the saline sites. These decreasing effects from upstream to the mouth were associated with the increase in urbanization as sites surrounded by urban areas released on average almost two times more CO2 and N2O than sites surrounded by nature and industry areas. Applied machine learning methods also revealed that, in addition to salinity effects, nutrient and organic enrichment stimulated the GHG emissions from the Scheldt Estuary. These findings highlight the importance of the interaction between salinity, water pollution, and land use in order to understand their influences on GHG emissions from dynamic estuarine systems.

The recovery of European freshwater biodiversity has come to a halt.

Owing to a long history of anthropogenic pressures, freshwater ecosystems are among the most vulnerable to biodiversity loss1. Mitigation measures, including wastewater treatment and hydromorphological restoration, have aimed to improve environmental quality and foster the recovery of freshwater biodiversity2. Here, using 1,816 time series of freshwater invertebrate communities collected across 22 European countries between 1968 and 2020, we quantified temporal trends in taxonomic and functional diversity and their responses to environmental pressures and gradients. We observed overall increases in taxon richness (0.73% per year), functional richness (2.4% per year) and abundance (1.17% per year). However, these increases primarily occurred before the 2010s, and have since plateaued. Freshwater communities downstream of dams, urban areas and cropland were less likely to experience recovery. Communities at sites with faster rates of warming had fewer gains in taxon richness, functional richness and abundance. Although biodiversity gains in the 1990s and 2000s probably reflect the effectiveness of water-quality improvements and restoration projects, the decelerating trajectory in the 2010s suggests that the current measures offer diminishing returns. Given new and persistent pressures on freshwater ecosystems, including emerging pollutants, climate change and the spread of invasive species, we call for additional mitigation to revive the recovery of freshwater biodiversity.

Individual variation in the habitat selection of upstream migrating fish near a barrier.

BACKGROUND: Migration is a vital element of the life cycle of many freshwater fish species but is increasingly hampered globally by riverine barriers. Fish passes are a common approach to enable migration past barriers but are often ineffective. More knowledge is required on fish behaviour as they approach barriers such as habitat preferences. METHODS: We evaluate the habitat selection of two upstream migrating fish species, barbel Barbus barbus and grayling Thymallus thymallus, at a hydropower plant in southern Germany, considering individual variation and population trends. Fish were tracked via fine-scale 2D acoustic telemetry in 2018 during their spawning migration. Step selection functions were used to evaluate selection of hydraulic parameters by the fish for a time step of 20 s. Exploratory models were built via model selection for each individual fish, to evaluate the extent of individual variation in model structure. A population model was developed for each species by averaging coefficients from individual models to describe general trends. The extent of individual variation was determined and confidence intervals for the population model coefficients were calculated. RESULTS: Fish varied greatly in individual model structure though common terms were apparent in both species, such as depth, flow velocity, the angular difference between fish and velocity, and the logarithm of the step length. Final population models for barbel included several parameters describing habitat selection and displacement. Barbel selected for faster flows, deeper water, and higher spatial velocity gradients. In addition, they selected to move more with the flow than against. Interactions were also present between habitat parameters, suggesting selection is context dependent. Barbel movement speed also changed with depth, flow velocity and spatial velocity gradient. With grayling, terms often had contrasting effects among individuals and thus general trends could not be distinguished for most terms. CONCLUSION: Our findings demonstrate habitat selection by upstream migrating fish approaching a fish pass and differences in individual selection which may have an impact on barrier management. Step selection functions are a promising approach and can provide useful insight into habitat selection and movement by migrating freshwater fish in an altered river system.

Pesticide concentration in three selected fish species and human health risk in the Lake Tana sub-basin, Ethiopia.

Pesticide use has increased in the Lake Tana sub-basin due to increased agricultural activity, potentially endangering nontargeted organisms. To assess its potential impact on fish health and fish-consuming human populations, pesticide concentrations in the fillet and liver tissue of three fish species, namely Labeobarbus megastoma, Labeobarbus tsanensis, and Oreochromis niloticus, were investigated in Lake Tana. Fish samples were taken from the lake near the rivers of Ribb and Gumara, which flow through agricultural areas where considerable amounts of pesticides have been applied. A total of 96 fish samples were collected. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) revealed the presence of ten pesticides. Pyrimethanil was frequently detected in 96% of liver and 65% of fillet samples at a median concentration of 33.9 µg kg-1 and 19.7 µg kg-1, respectively. The highest concentration of pyrimethanil was found in L. megastoma (1850.0 µg kg-1). Labeobarbus megastoma also had the highest concentration of oxamyl (507.0 µg kg-1) and flazasulfuron (60.1 µg kg-1) detected in the liver tissue. The highest concentration of carbaryl (56.5 µg kg-1) was found in the liver tissue of O. niloticus. Fish tissue samples from the two study sites contained pyrimethanil, oxamyl, carbaryl, and flazasulfuron. Only pyrimethanil showed a statistically significant difference between the two sites and the species L. megastoma and L. tsanensis. The amounts of pesticides found in the fish species pose no direct risk to the health of fish consumer human population. However, the results show that the lake ecosystem needs immediate attention and regular monitoring of the rising pesticide usage in the lake watershed.

Determining Tipping Points and Responses of Macroinvertebrate Traits to Abiotic Factors in Support of River Management.

Although the trait concept is increasingly used in research, quantitative relations that can support in determining ecological tipping points and serve as a basis for environmental standards are lacking. This study determines changes in trait abundance along a gradient of flow velocity, turbidity and elevation, and develops trait-response curves, which facilitate the identification of ecological tipping points. Aquatic macroinvertebrates and abiotic conditions were determined at 88 different locations in the streams of the Guayas basin. After trait information collection, a set of trait diversity metrics were calculated. Negative binomial regression and linear regression were applied to relate the abundance of each trait and trait diversity metrics, respectively, to flow velocity, turbidity and elevation. Tipping points for each environmental variable in relation to traits were identified using the segmented regression method. The abundance of most traits increased with increasing velocity, while they decreased with increasing turbidity. The negative binomial regression models revealed that from a flow velocity higher than 0.5 m/s, a substantial increase in abundance occurs for several traits, and this is even more substantially noticed at values higher than 1 m/s. Furthermore, significant tipping points were also identified for elevation, wherein an abrupt decline in trait richness was observed below 22 m a.s.l., implying the need to focus water management in these altitudinal regions. Turbidity is potentially caused by erosion; thus, measures that can reduce or limit erosion within the basin should be implemented. Our findings suggest that measures mitigating the issues related to turbidity and flow velocity may lead to better aquatic ecosystem functioning. This quantitative information related to flow velocity might serve as a good basis to determine ecological flow requirements and illustrates the major impacts that hydropower dams can have in fast-running river systems. These quantitative relations between invertebrate traits and environmental conditions, as well as related tipping points, provide a basis to determine critical targets for aquatic ecosystem management, achieve improved ecosystem functioning and warrant trait diversity.

Epigenetic analytical approaches in ecotoxicological aquatic research.

Environmental epigenetics has become a key research focus in global climate change studies and environmental pollutant investigations impacting aquatic ecosystems. Specifically, triggered by environmental stress conditions, intergenerational DNA methylation changes contribute to biological adaptive responses and survival of organisms to increase their tolerance towards these conditions. To critically review epigenetic analytical approaches in ecotoxicological aquatic research, we evaluated 78 publications reported over the past five years (2016-2021) that applied these methods to investigate the responses of aquatic organisms to environmental changes and pollution. The results show that DNA methylation appears to be the most robust epigenetic regulatory mark studied in aquatic animals. As such, multiple DNA methylation analysis methods have been developed in aquatic organisms, including enzyme restriction digestion-based and methyl-specific immunoprecipitation methods, and bisulfite (in)dependent sequencing strategies. In contrast, only a handful of aquatic studies, i.e. about 15%, have been focusing on histone variants and post-translational modifications due to the lack of species-specific affinity based immunological reagents, such as specific antibodies for chromatin immunoprecipitation applications. Similarly, ncRNA regulation remains as the least popular method used in the field of environmental epigenetics. Insights into the opportunities and challenges of the DNA methylation and histone variant analysis methods as well as decreasing costs of next generation sequencing approaches suggest that large-scale epigenetic environmental studies in model and non-model organisms will soon become available in the near future. Moreover, antibody-dependent and independent methods, such as mass spectrometry-based methods, can be used as an alternative epigenetic approach to characterize global changes of chromatin histone modifications in future aquatic research. Finally, a systematic guide for DNA methylation and histone variant methods is offered for ecotoxicological aquatic researchers to select the most relevant epigenetic analytical approach in their research.

Occurrence Prediction of Riffle Beetles (Coleoptera: Elmidae) in a Tropical Andean Basin of Ecuador Using Species Distribution Models.

Genera and species of Elmidae (riffle beetles) are sensitive to water pollution; however, in tropical freshwater ecosystems, their requirements regarding environmental factors need to be investigated. Species distribution models (SDMs) were established for five elmid genera in the Paute river basin (southern Ecuador) using the Random Forest (RF) algorithm considering environmental variables, i.e., meteorology, land use, hydrology, and topography. Each RF-based model was trained and optimised using cross-validation. Environmental variables that explained most of the Elmidae spatial variability were land use (i.e., riparian vegetation alteration and presence/absence of canopy), precipitation, and topography, mainly elevation and slope. The highest probability of occurrence for elmids genera was predicted in streams located within well-preserved zones. Moreover, specific ecological niches were spatially predicted for each genus. Macrelmis was predicted in the lower and forested areas, with high precipitation levels, towards the Amazon basin. Austrelmis was predicted to be in the upper parts of the basin, i.e., páramo ecosystems, with an excellent level of conservation of their riparian ecosystems. Austrolimnius and Heterelmis were also predicted in the upper parts of the basin but in more widespread elevation ranges, in the Heterelmis case, and even in some areas with a medium level of anthropisation. Neoelmis was predicted to be in the mid-region of the study basin in high altitudinal streams with a high degree of meandering. The main findings of this research are likely to contribute significantly to local conservation and restoration efforts being implemented in the study basin and could be extrapolated to similar eco-hydrological systems.

A comprehensive assessment of plastic remediation technologies.

The global presence of plastic litter and its accumulation in the environment has become an issue of concern to the public and policymakers. This concern has triggered innovators in past decades to design and develop a multitude of remediation technologies to prevent plastic from entering the environment, or to clean up legacy litter. This study aims to (i) systematically review the current scientific literature on plastic remediation technologies, (ii) create a 'plastic clean-up and prevention overview' illustrating 124 remediation technologies and 29 characteristics, (iii) qualitatively analyse their key characteristics (e.g., fields of application, targeted plastic), and (iv) investigate challenges and opportunities of clean-up technologies for inland waterways (e.g., canals, rivers) and ports. We identified 61 scientific publications on plastic remediation technologies, until June 2022. Thirty-four of these studies were published within the last three years, demonstrating a growing interest. The presented overview indicates that inland waterways are, so far, the preferred field of application, with 22 technologies specifically designed for cleaning up plastics from inland waterways, and 52 additional ones with the potential to be installed in these locations. Given the importance of clean-up technologies in inland waterways, we highlighted their strengths, weaknesses, opportunities, and threats (SWOT). Our results indicate that, despite the challenges, these technologies provide essential prospects, from improving the environmental quality to raising awareness. Our study is instrumental as it illustrates an up-to-date overview and provides a comprehensive analysis of current in design phase, testing, and in use plastic remediation technologies.

Freshwater Management Discourses in the Northern Peruvian Andes: The Watershed-Scale Complexity for Integrating Mining, Rural, and Urban Stakeholders.

The Peruvian environmental action plan seeks headwaters protection as one of its integrated watershed management objectives. However, heterogeneous social and environmental conditions shape this freshwater management challenge at subnational scales. We have noticed different interpretations of this challenge. To map the debate, understand the diverse interpretations, and frame political choices, we conducted semi-structured interviews with institutional and non-institutional stakeholders for performing discourse analysis in an Andean watershed where mountaintop gold mining, midstream farmers, and the downstream Cajamarca city coexist. One discourse dominates the debate on protecting the freshwater supply and argues the importance of river impoundment, municipal storage capacity, and institutional leadership. The other two discourses revolve around protecting the mountain aquifer. The second discourse does so with a fatalistic view of headwaters protection and rural support. The third discourse partially shifts the debate towards the need for improving rural capacity building and (ground)water inventories. To understand evolutions in society, it is crucial to understand these three discourses, including the types of knowledge that actors present as legitimate, the attributed roles to all stakeholders, and the kinds of worldviews informing each discourse. The interaction among discourses could hinder integrated watershed management at worst or, at best, help inspire multi-stakeholder collaboration.

Individual based models for the simulation of fish movement near barriers: Current work and future directions.

River fragmentation is an increasing issue for water managers and conservationists. Barriers such as dams interfere with freshwater fish migration, leading to drastic population declines. While there are a range of widely implemented mitigation approaches, e.g. fish passes, such measures are often inefficient due to suboptimal operation and design. There is increasing need to be able to assess mitigation options prior to implementation. Individual based models (IBMs) are a promising option. IBMs can simulate the fine-scale movement of individual fish within a population as they attempt to find a fish pass, incorporating movement processes themselves. Moreover, IBMs have high transferability to other sites or conditions (e.g. changing mitigation, change in flow conditions), making them potentially valuable for freshwater fish conservation yet their application to the fine-scale movement of fish past barriers is still novel. Here, we present an overview of existing IBMs for fine-scale freshwater fish movement, with emphasis on study species and the parameters driving movement in the models. In this review, we focus on IBMs suitable for the simulation of fish tracks as they approach or pass a single barrier. The selected IBMs for modelling fine-scale freshwater fish movement largely focus on salmonids and cyprinid species. IBMs have many applications in the context of fish passage, such as testing different mitigation options or understanding processes behind movement. Existing IBMs include movement processes such as attraction and rejection behaviours, as reported in literature. Yet some factors affecting fish movement e.g. biotic interactions are not covered by existing IBMs. As the technology available for fine scale data collection continues to advance, such as increasing data linking fish behaviour to hydraulics, IBMs could become a more common tool in the design and implementation of fish bypass structures.

Widespread triazole pesticide use affects infection dynamics of a global amphibian pathogen.

The sixth mass extinction is a consequence of complex interplay between multiple stressors with negative impact on biodiversity. We here examine the interaction between two globally widespread anthropogenic drivers of amphibian declines: the fungal disease chytridiomycosis and antifungal use in agriculture. Field monitoring of 26 amphibian ponds in an agricultural landscape shows widespread occurrence of triazole fungicides in the water column throughout the amphibian breeding season, together with a negative correlation between early season application of epoxiconazole and the prevalence of chytrid infections in aquatic newts. While triazole concentrations in the ponds remained below those that inhibit growth of Batrachochytrium dendrobatidis, they bioaccumulated in the newts' skin up to tenfold, resulting in cutaneous growth-suppressing concentrations. As such, a concentration of epoxiconazole, 10 times below that needed to inhibit fungal growth, prevented chytrid infection in anuran tadpoles. The widespread presence of triazoles may thus alter chytrid dynamics in agricultural landscapes.

Health risk-benefit assessment of the commercial red mangrove crab: Implications for a cultural delicacy.

Mangrove forests, provide vital food resources and are an endangered ecosystem worldwide due to pollution and habitat destruction. A risk-benefit assessment (RBA) was performed on the red mangrove crab (Ucides occidentalis) from the threatened Guayas mangroves in Ecuador. It was aimed to assess the combined potential adverse and beneficial health impact associated with crab consumption and define a recommended safe intake (SI) to improve the diet of the Ecuadoran population while ensuring safe food intake. Target hazard quotients (THQs), benefit quotients (Qs), and benefit-risk quotients (BRQs) were calculated based on the concentrations of the analyzed contaminants (121 pesticide residues, 11 metal(loid)s, antimicrobial drugs from 3 classes) and nutrients (fatty acids, amino acids, and essential nutrients). Except for inorganic arsenic (iAs), the THQ was below 100 for all investigated contaminants, suggesting that the average crab consumer is exposed to levels that do not impose negative non-carcinogenic or carcinogenic health effects in the long and/or short term. Concentrations of iAs (average AsIII: 25.64 and AsV: 6.28 µg/kg fw) were of the highest concern because of the potential to cause negative health effects on long-term consumption. Despite the thriving aquaculture in the Guayas estuary, concentrations of residues of the antimicrobial drugs oxytetracycline (OTC), florfenicol, and nitrofurans still were low. Based on the fact that different risk reference values exist, related to different safety levels, four SI values (0.002, 0.04, 4, and 18 crabs/day) were obtained. The strictest intake values indicate a concern for current consumption habits. In conclusion, the red mangrove crab contains various important nutrients and can be part of a balanced diet for the Ecuadorian population when consumed in limited portions. The present study emphasizes the importance of safeguarding the quality of the environment as a prerequisite for procuring nutritious and safe food.

The nutritional quality of the red mangrove crab (Ucides occidentalis), harvested at two reserves in the Guayas estuary.

Crabs are an outstanding source of many essential nutrients. Little research has been performed on the nutritional quality of the red mangrove crab (Ucides occidentalis). In this research we investigated the proximate composition, fatty acid profile, amino acid composition, and mineral concentrations of red mangrove crabs sampled at two reserves within the Guayas estuary in Ecuador. Furthermore, we evaluated the influence of spatial variation. We found that the red mangrove is a good source of nutrition for a balanced diet and can contribute to the recommended intake of essential nutrients. Also, the red mangrove crab obtained better lipid nutritional quality indices compared to other shellfish species, apart from the EPA + DHA index values. Current research indicated that the final nutritional quality indices of the red crab were not influenced by harvest site. More research into the influence of environmental and ecological factors on the nutritional composition of crabs is suggested.