Pasture Minerals for Dairy Cattle.

While mineral requirements do not differ with the production systems, the forage bases of the different dairy production systems influence the risk of mineral deficiency. Testing representative pastures on a farm is a key to understanding the potential for risk of mineral deficiency and should be combined with blood or tissue samples, clinical observation, and responses to treatment to evaluate the need for supplementation.

A meta-analysis of effects of dietary seaweed on beef and dairy cattle performance and methane yield.

There has been considerable interest in the use of red seaweed, and in particular Asparagopsis taxiformis, to increase production of cattle and to reduce greenhouse gas emissions. We hypothesized that feeding seaweed or seaweed derived products would increase beef or dairy cattle performance as indicated by average daily gain (ADG), feed efficiency measures, milk production, and milk constituents, and reduce methane emissions. We used meta-analytical methods to evaluate these hypotheses. A comprehensive search of Google Scholar, Pubmed and ISI Web of Science produced 14 experiments from which 23 comparisons of treatment effects could be evaluated. Red seaweed (Asparagopsis taxiformis) and brown seaweed (Ascophyllum nodosum) were the dominant seaweeds used. There were no effects of treatment on ADG or dry matter intake (DMI). While there was an increase in efficiency for feed to gain by 0.38 kg per kg [standardized mean difference (SMD) = 0.56; P = 0.001] on DerSimonian and Laird (D&L) evaluation, neither outcome was significant using the more rigorous robust regression analysis (P >0.06). The type of seaweed used was not a significant covariable for ADG and DMI, but A. nodosum fed cattle had lesser feed to gains efficiency compared to those fed A. taxiformis. Milk production was increased with treatment on weighted mean difference (WMD; 1.35 ± 0.44 kg/d; P <0.001); however, the SMD of 0.45 was not significant (P = 0.111). Extremely limited data suggest the possibility of increased percentages of milk fat (P = 0.040) and milk protein (P = 0.001) on (D&L) WMD evaluation. The limited data available indicate dietary supplementation with seaweed produced a significant and substantial reduction in methane yield by 5.28 ± 3.5 g/kg DMI (P = 0.003) on D&L WMD evaluation and a D&L SMD of -1.70 (P = 0.001); however, there was marked heterogeneity in the results (I2 > 80%). In one comparison, methane yield was reduced by 97%. We conclude that while there was evidence of potential for benefit from seaweed use to improve production and reduce methane yield more in vivo experiments are required to strengthen the evidence of effect and identify sources of heterogeneity in methane response, while practical applications and potential risks are evaluated for seaweed use.

Effects of hormonal growth promotants on beef quality: a meta-analysis.

Benefits of hormonal growth promotants (HGPs) include production efficiency, profit, and reduced environmental effects for beef cattle. Questions remain about effects of HGP on beef quality, particularly on measures of toughness such as Warner-Bratzler shear force (WBSF), tenderness, and other taste-panel attributes of beef. The objective of this meta-analysis was to assess the effects of HGP on beef quality using the results of randomized controlled trials identified from 3 searched databases. Thirty-one experiments with 181 treatment comparisons were used to evaluate the effects of HGP on WBSF and sensory measures of beef quality. Experiments varied in design, used many different hormonal treatments and combinations, which were single or repeated, in different breeds and sex groups of cattle, with or without electrical stimulation, and with different lengths of time on feed and beef aging. The effects of multiple treatment comparisons in experiments were evaluated using robust regression models and compared to Knapp-Hartung and permutation meta-analytical methods. Increased WBSF was associated with HGP treatment. Use of multiple HGP implants was associated with an increase in WBSF of 0.248 kg (95% CI = 0.203 to 0.292). Effects of a single implant only increased WBSF by 0.176 kg (95% CI = 0.109 to 0.242). Aging of beef did not alter the association of HGP with increased WBSF (P = 0.105); however, the point direction was toward a reduced effect with aging (standardized mean difference [SMD] = -0.005 per day aged). While aging lowered WBSF, it did not reduce the SMD between HGP treatment and reference groups. Comparisons using trenbolone acetate did not differ in WBSF from those using other implants (P > 0.15). The findings on sensory panel tenderness differ from those using WBSF as HGP treatment was not associated with reduced tenderness (P > 0.3) and multiple HGP treatments improved tenderness (SMD = 0.468) compared to a single implant. Further, juiciness, flavor, and connective tissue were not associated with HGP use, whereas there was a marked 5.5-point decrease in the Meat Standards Australia meat quality 4 score, albeit with limited experiments. In general, the true variance of experiments, tau2 (τ2) was low (<0.1), but heterogeneity, I2 was high (>50%) indicating that much of the variance was due to factors other than measurement error. More targeted studies on the role of HGP in influencing beef quality are needed.

Genetic Markers Are Associated with the Ruminal Microbiome and Metabolome in Grain and Sugar Challenged Dairy Heifers.

Dairy heifers were subjected to a non-life-threatening challenge designed to induce ruminal acidosis by feeding grain and sugar. Large among animal variation in clinical signs of acidosis, rumen metabolite concentrations, and the rumen microbiome occurred. This exploratory study investigates sources of the variation by examining associations between the genome, metabolome, and microbiome, albeit with a limited population. The broader objective is to provide a rationale for a larger field study to identify markers for susceptibility to ruminal acidosis. Initially, heifers (n = 40) allocated to five feed additive groups were fed 20-days pre-challenge with a total mixed ration and additives. Fructose (0.1% of bodyweight/day) was added for the last 10 days pre-challenge. On day 21 heifers were challenged with 1.0% of bodyweight dry matter wheat + 0.2% of bodyweight fructose + additives. Rumen samples were collected via stomach tube weekly (day 0, 7, and 14) and at five times over 3.6 h after challenge and analyzed for pH and volatile fatty acid, ammonia, D-, and L-lactate concentrations. Relative abundance of bacteria and archaea were determined using Illumina MiSeq. Genotyping was undertaken using a 150K Illumina SNPchip. Genome-wide association was performed for metabolite and microbiome measures (n = 33). Few genome associations occurred with rumen pH, concentration of acetate, propionate, total volatile fatty acids, or ammonia, or the relative abundance of the Firmicutes, Bacteroidetes, and Spirochaetes phyla. Metabolites and microbial phyla that had markers associated and quantitative trait loci (QTL) were: acetate to propionate ratio (A:P), D-, L-, and total lactate, butyrate, acidosis eigenvalue, Actinobacteria, Chloroflexi, Euryarchaeota, Fibrobacteres, Planctomycetes, Proteobacteria, and Tenericutes. A putative genomic region overlapped for Actinobacteria, Euryarchaeota, and Fibrobacteres and covered the region that codes for matrix extracellular phosphoglycoprotein (MEPE). Other overlapping regions were: (1) Chloroflexi, Tenericutes, and A:P, (2) L- and total lactate and Actinobacteria, and (3) Actinobacteria, Euryarchaeota, Fibrobacteres, and A:P. Genome-wide associations with the metabolome and microbiome occurred despite the small population, suggesting that markers for ruminal acidosis susceptibility exist. The findings may explain some of the variation in metabolomic and microbial data and provide a rationale for a larger study with a population that has variation in acidosis.

Feeding, evaluating, and controlling rumen function.

Achieving optimal rumen function requires an understanding of feeds and systems of nutritional evaluation. Key influences on optimal function include achieving good dry matter intake. The function of feeds in the rumen depends on other factors including chemical composition, rate of passage, degradation rate of the feed, availability of other substrates and cofactors, and individual animal variation. This article discusses carbohydrate, protein, and fat metabolism in the rumen, and provides practical means of evaluation of rations in the field. Conditions under which rumen function is suboptimal (ie, acidosis and bloat) are discussed, and methods for control examined.

Ruminal acidosis in a 21-month-old Holstein heifer.

Rumen and blood biochemical profiles were monitored in 8 Holstein heifers exposed to a carbohydrate feeding challenge. One of the heifers had clinical signs consistent with acute ruminal acidosis on the day of, and subsequent to, the challenge. Within 24 h of challenge, 6 of 7 rumen volatile fatty acids measured were not detectable in this heifer and her rumen total lactate concentration was > 70 mM.

Acidose spumeuse chez une génisse Holstein âgée de 21 mois. Les profils du rumen et de l'hématologie biochimiques ont été surveillés chez 8 génisses Holstein exposées à une épreuve d'alimentation en glucides. L'une des génisses avaient des signes cliniques conformes à une acidose spumeuse aiguë le jour et le lendemain de l'épreuve. Dans un délai de 24 h après l'épreuve, 6 des 7 acides gras volatils du rumen mesurés n'étaient pas détectables chez cette génisse et la concentration de lactate totale de son rumen était de > 70 mM.(Traduit par Isabelle Vallières).