Does hyperconcentration result in platelet activation?. A flow-cytometric study of hyperconcentrated random donor platelets.

BACKGROUND AND OBJECTIVES: Hyperconcentration of platelets may lead to platelet activation and loss of platelet function. MATERIALS AND METHODS: Platelet activation following hyperconcentration was assessed using flow-cytometric detection of platelet P-selectin expression and platelet swirling. RESULTS: Platelet hyperconcentration led to a minimal increase in P-selectin expression and no difference in platelet swirling. CONCLUSION: Hyperconcentration was not associated with a clinically significant change in platelet activation and had no significant effect on platelet quality as detected by pH and platelet yield.