RESUMEN
Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4%) produced metallo-beta-lactamase, while 26 (74.3%) showed the bla(SPM-1) gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.
Asunto(s)
Farmacorresistencia Bacteriana Múltiple/genética , Pseudomonas aeruginosa/enzimología , beta-Lactamasas/genética , Antibacterianos/farmacología , Brasil , Infección Hospitalaria/microbiología , Pruebas Antimicrobianas de Difusión por Disco , Humanos , Fenotipo , Reacción en Cadena de la Polimerasa , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/genética , beta-Lactamasas/biosíntesisRESUMEN
Pseudomonas aeruginosa é uma bactéria frequentemente isolada no ambiente hospitalar. Este estudo teve como objetivo avaliar o perfil de suscetibilidade de Pseudomonas aeruginosa previamente isoladas de pacientes internados em um hospital de Goiânia (Goiás-Brasil); realizar a triagem fenotípica para a produção de metalo-beta-lactamase e detectar os genes das mesmas pela técnica de "Polimerase Chain Reaction". Foram avaliadas 75 Pseudomonas aeruginosa isoladas no período de janeiro de 2005 a janeiro de 2007. A identificação bioquímica foi realizada pelo sistema API 20E® e o antibiograma pelo método de Kirby-Bauer. Entre os 62 isolados que foram resistentes ao imipenem e à ceftazidima, 35 (56,4 por cento) apresentaram produção de metalo-beta-lactamase e em 26 (74,3 por cento) destes, foi detectado o gene blaSPM-1. A frequência de Pseudomonas aeruginosa produtoras de metalo-beta-lactamase sugere um maior controle da disseminação de resistência no ambiente hospitalar.
Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E® system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4 percent) produced metallo-beta-lactamase, while 26 (74.3 percent) showed the blaSPM-1 gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.