RESUMEN
As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immunoassay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99.5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.
Asunto(s)
Esquistosomiasis/diagnóstico , Adolescente , Adulto , Anciano , Anticuerpos/análisis , Recolección de Muestras de Sangre , Niño , Preescolar , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunoglobulina G/análisis , Masculino , Persona de Mediana Edad , Recuento de Huevos de Parásitos , Schistosoma mansoni/inmunología , Esquistosomiasis/inmunología , Esquistosomiasis/parasitología , Indias OccidentalesRESUMEN
As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantative features of an enzyme linked immunoassay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99.5 percent sensitivity, depending on the level of infection intensity and method of blood collection and 100 percent specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area (AU)