Characterization and antibacterial evaluation of Eco-friendly silver nanoparticles synthesized by halophilic Streptomyces rochei SSCM102 isolated from mangrove sediment.

BACKGROUND: Antimicrobial resistance has surged due to widespread antimicrobial drug use, prompting interest in biosynthesizing nanoparticles from marine-derived actinomycetes extracellular metabolites, valued for their diverse bioactive compounds. This approach holds promise for addressing the urgent need for novel antimicrobial agents. The current study aimed to characterize novel bioactive compounds from unexplored biodiversity hotspots, halophilic Streptomyces sp. isolated from mangrove sediment in the Pichavaram region, India. METHODS AND RESULTS: Streptomyces rochei SSCM102 was conclusively identified through morphological and molecular characterization. Synthesis of silver nanoparticles (AgNPs) from Streptomyces rochei SSCM102 was characterized using various techniques, including UV-Vis, XRD, SEM, EDX, and FT-IR. The UV-Vis spectrum of the reduced AgNPs exhibited a prominent peak at 380 nm, confirming the AgNPs. The UV-Vis spectrum confirmed the synthesis of AgNP, and SEM analysis revealed a cubic morphology with sizes ranging from 11 to 21 nm. The FTIR spectrum demonstrated a shift in frequency widths between 626 cm-1 and 3432 cm-1. The EDX analysis substantiated the presence of metallic silver, evident from a strong band at 1.44 keV. The synthesized AgNPs exhibited antibacterial efficacy against human pathogens Escherichia coli (64 ± 0.32 µg/ml), Klebsiella pneumoniae (32 ± 0.16 µg/ml), and Pseudomonas aeruginosa (16 ± 0.08 µg/ml) by MIC and MBC values of 128 ± 0.64 (µg/ml), 64 ± 0.32 (µg/ml) and 32 ± 0.16 (µg/ml), respectively. Additionally, at a concentration of 400 µg/ml, the AgNPs displayed a 72% inhibition of DPPH radicals, indicating notable antioxidant capacity. The LC50 value of 130 µg/mL indicates that the green-synthesized AgNPs have lower toxicity by Brine Shrimp Larvae assay. CONCLUSION: The study's novel approach to synthesizing eco-friendly silver nanoparticles using Halophilic Streptomyces rochei SSCM102 contributes significantly to the field of biomedical research and drug development. By demonstrating potent antibacterial properties and aligning with sustainability goals, these nanoparticles offer promising avenues for novel antibacterial therapies.

Data set on impact of COVID-19 on mental health of internal migrant workers in India: Corona Virus Anxiety Scale (CAS) approach.

The article presents a unique dataset on mental health of internal Migrant workers in India. The dataset was constructed during the pandemic when the entire nation was the victim of stringent measures to curtail the spread of Corona Virus in the form of travel restrictions and lockdowns. We collected this data in our pursuit to submit a paper in response to call for paper in the Journal titled "Migration and health". Non-availability of authentic data about the internal migrant workers triggered this effort to compile the data. We have recorded 1350 responses out of a 6897 Sample through snowball sampling method. Every respondent is said to be a referee for further driving of sample. The responses were collected between June 2 and August 30, 2020 through the telephonic interviews. Also, the consent of the respondents has been duly obtained for publication of the data without revealing their identity. The interview schedule was adopted by using Corona virus Anxiety Scale (CAS) which uses four dimension model namely Cognitive, Emotional, Behavioral and Psychological. The Interview schedule was originally designed in English but was later translated into three different languages after consulting the language experts. This article provides descriptive statistics of study variables along with socio economic factors. This dataset provides a significant platform for further research related to CAS and in assessment of mental health of vulnerable groups.