[Immunobiologic characteristics of hybrid proteins consisting of tumor necrosis factor alpha and thymosin alpha 1]. / Izuchenie immunobiologicheskikh svoistv gibridnykh belkov sostoiashchikh iz faktora nekroza opukholei alpha i timozina alpha 1.

The preparations of alpha 1-thymosin (T), alpha-tumor necrotic factor (TNF) and their based hybrid proteins: T-TNF, TNF-T, and T-TNF-T were studied by using a wide spectrum of immunobiological tests. In the L-929 cells, T-TNF preserved cytotoxicity unique to TNF; TNF-T preserved it 10 times less, and T-NTF-T was completely inactive. TNF-T inhibited the growth of Molt-4, Jm-9, Raji cells by 63 = 84%, and TNF suppressed only Raji cells by 50%. Hybrid proteins caused cell proliferation of the thymus, spleen, and lymph nodes; H-2K, CD4, and CD8 differently increased the expression of thymocytic antigens. The authors found the different effects of the drugs on phagocytosis, the production of antibody-forming cells, delayed reaction, and activation of natural killer cells. The protein T-TNF-T produced the most pronounced action.

[Cytostatic action of the peritoneal cells of Syrian hamsters to transformed and tumor cells in vitro and its suppression by prostaglandin E2]. / Tsitostaticheskoe deistvie peritoneal'nykh kletok siriiskikh khomiakov na transformirovannye i opukholevye kletki in vitro i ego podavlenie prostaglandinom E2.

High antitumour activity of Syrian hamster peritoneal cells (PC) is demonstrated in the cytostatic test in vitro. The cytostatic effect (CSE) of PC on hamster cell strains can be revealed at the very early stage (7 hours of cocultivation) and it remains stable during at least 24 hours. Single intraperitoneal injection of physiological (10(-9) M) or pharmacological (10(-7)) doses of prostaglandin E2 preparation leads to the prolonged and significant inhibition of CSE of PC.