Functional response of an egg parasitoid, Trichogramma chilonis Ishii to sublethal imidacloprid exposure.

BACKGROUND: The effectiveness of a biological control agent depends on how well it can control pests and how compatible it is with pesticides. Therefore, we reported the multigenerational effect of a commonly used insecticide, imidacloprid, on the functional response of a widely acclaimed egg parasitoid, Trichogramma chilonis Ishii, to different densities of the host Corcyra cephalonica Stainton eggs. The study investigated the outcomes of the median lethal concentration (LC50 ) and sublethal concentrations (LC5 , LC30 ), along with control treatments for five continuous generations (F1 to F5 ). RESULTS: The results showed that the F5 generation of LC30 , both of the F1 and F5 generations of LC50 , and the control all had a Type II functional response. A Type I functional response was exhibited for the F1 generation of LC30 and both generations of LC5 . The attack rate on host eggs treated with LC5 and LC30 did not change (decrease) with the shift in the type of functional response as compared to the control. A significant increase in the searching efficiency (a) was observed in the later generation (F5 ) under the exposure of LC5 and LC30 imidacloprid concentrations. A lower handling time (Th ) in both generations of the LC5 followed by LC30 treated individuals was observed when compared with the control and LC50 treatments. The per capita parasitization efficiency (1/Th ) and the rate of parasitization per handling time (a/Th ) were also considerably higher in both the generations of LC5 and LC30 than in the control and LC50 , thereby implying positive effects of imidacloprid on the parasitization potential of T. chilonis. CONCLUSION: Altogether, these multigenerational outcomes on the functional response of T. chilonis could be leveraged to control the intractable lepidopteran pests under the mild exposure of imidacloprid in integrated pest management (IPM) programs as well as in the mass rearing of the parasitoid, T. chilonis. © 2023 Society of Chemical Industry.

Sublethal phosphine fumigation induces transgenerational hormesis in a factitious host, Corcyra cephalonica.

BACKGROUND: The rice moth, Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) is a pest of stored grains and widely used as a factitious host during the mass rearing of several natural enemies of crop pests. Hormesis is well-documented in pest insects, to some extent in natural enemies of pests. RESULTS: We report transgenerational stimulatory effects of the widely used fumigant, phosphine. The study reports the consequences of sublethal, low lethal and median lethal concentrations (LC5 , LC25 and LC50 ) and untreated control for two sequential generations of the species (G1 to G2 ). In this study, we investigated the life-history traits, nutrient reserves (protein, lipid and carbohydrate) and larval gut microbiome (using 16 s rRNA V3-V4 metagenomics sequencing) of C. cephalonica. Stimulatory effects were observed for various biological traits of C. cephalonica, notably adult longevity, emergence and increased egg hatchability when exposed to LC5 of phosphine. The total protein, lipid and carbohydrate contents of C. cephalonica also were found to be significantly increased by LC5 in both generations. The microbial diversity of LC5 treated larval gut was higher and found to be different from the rest of the treatments. This is the first report showing hormesis to a fumigant insecticide. CONCLUSION: Our findings increase knowledge on the interaction between hormesis, nutrient reserves and gut bacteria in C. cephalonica exposed to insecticides. Overall, the present study establishes phosphine-induced hormesis at LC5 in the host C. cephalonica, which might help improve the quality of mass rearing of various natural enemies. © 2023 Society of Chemical Industry.

Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS).

Nilaparvata lugens is the main rice pest in India. Until now, the Indian N. lugens mitochondrial genome has not been sequenced, which is a very important basis for population genetics and phylogenetic evolution studies. An attempt was made to sequence two examples of the whole mitochondrial genome of N. lugens biotype 4 from the Indian population for the first time. The mitogenomes of N. lugens are 16,072 and 16,081 bp long with 77.50% and 77.45% A + T contents, respectively, for both of the samples. The mitochondrial genome of N. lugens contains 37 genes, including 13 protein-coding genes (PCGs) (cox1-3, atp6, atp8, nad1-6, nad4l, and cob), 22 transfer RNA genes, and two ribosomal RNA (rrnS and rrnL) subunits genes, which are typical of metazoan mitogenomes. However, both samples of N. lugens mitogenome in the present study retained one extra copy of the trnC gene. Additionally, we also found 93 bp lengths for the atp8 gene in both of the samples, which were 60-70 bp less than that of the other sequenced mitogenomes of hemipteran insects. The phylogenetic analysis of the 19 delphacids mitogenome dataset yielded two identical topologies when rooted with Ugyops sp. in one clade, and the remaining species formed another clade with P. maidis and M. muiri being sisters to the remaining species. Further, the genus Nilaparvata formed a separate subclade with the other genera (Sogatella, Laodelphax, Changeondelphax, and Unkanodes) of Delphacidae. Additionally, the relationship among the biotypes of N. lugens was recovered as the present study samples (biotype-4) were separated from the three biotypes reported earlier. The present study provides the reference mitogenome for N. lugens biotype 4 that may be utilized for biotype differentiation and molecular-aspect-based future studies of N. lugens.

Deciphering the associated risk on soil microbes upon use of biopesticides in rice ecosystem.

Plant species, viz Cleistanthus collinus, Lantana camara, and Strychnos nux-vomica are being traditionally used for pest management in rice. However, limited investigation has been carried out to understand the toxic effect of these materials on soil microbes. Hot water extracts of these plants were evaluated for their effects on soil microbial population and enzyme activities along with neem oil and chlorpyrifos as check. Soil microbial population, viz bacteria, fungi, phosphate-solubilizing bacteria (PSB), and asymbiotic nitrogen fixers were unchanged after application of plant extracts. Maximum population of bacteria including PSB and asymbiotic nitrogen fixers were observed in control, whereas, S. nux-vomica, and C. collinus-treated soil had higher number of actinomycetes and fungal population, respectively. Soil microbial biomass did not vary differently among the plant extracts. Application of plant extracts did not alter dehydrogenase, ß-glycosidase, acid phosphatase, alkaline phosphatase, and urease content in soil. Secondary metabolites present in these plant extracts may be responsible for variable effects on soil microbes. Chlorpyrifos had a fleeting negative effect on soil microbes and enzymes in comparison to plant extracts. All the three plants did not have any negative effect on soil microbes and enzymes and can be safely recommended in rice pest management.