Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros











Base de datos
Idioma
Intervalo de año de publicación
1.
Prikl Biokhim Mikrobiol ; 11(5): 725-9, 1975.
Artículo en Ruso | MEDLINE | ID: mdl-241996

RESUMEN

Different methods of the preparation of fungal beta-galactosidase from the 72-hour culture of Alternaria tenuis were tested: lyophilization of the culture liquid, precipitation with ethanol, acetone, ammonium sulphate. Optimal results were obtained with precipitation by 1.5 acetone volume. Studies of the properties of fungal beta-galactosidase demonstrated that the preparation retained its activity during 22 month storage at 5 degrees C. The fungal preparation had pH optimum at a more acidic zone (4.2 versus 6.9), was active in a wider pH range 2.8-5.7 and 6.2-7.5), had a much higher temperature optimum (65 degrees and 30 degrees) and better thermostability as compared with the yeast preparation. Data on other properties of the preparation are presented.


Asunto(s)
Alternaria/enzimología , Galactosidasas/aislamiento & purificación , Hongos Mitospóricos/enzimología , Medios de Cultivo , Diálisis , Estabilidad de Medicamentos , Activación Enzimática , Liofilización , Galactosidasas/farmacología , Concentración de Iones de Hidrógeno , Solventes , Temperatura , Factores de Tiempo , Ultracentrifugación
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA