RESUMEN
Pesticides products are widely used to increase food productivity and to decrease food-borne diseases. Fludioxonil is a worldwide used phenylpyrrol fungicide. This pesticide can induce serious effects on human health especially on nervous system. We assessed the role of oxidative stress in the toxicity of Fludioxonil and examined its apoptotic mechanism of action on rat neural cells (F98). We have shown that the increasing concentration of Fludioxonil reduces the percentage of living F98 cells viability and increases the levels of reactive oxygen species and malondialdheydes. The reduction of cells proliferation was demonstrated with an accumulation in G2/M phase. The immunocytochemical analysis has shown that Fludioxonil induced the disruption of the cytoskeleton. DNA damage was also provoked in a concentration dependent manner as illustrated by the comet assay. The depolarization of the mitochondria and the positive Annexin V FITC-PI confirmed the apoptosis induced by this fungicide. Interestingly, the F98 cells viability and ROS levels were restored with N-acetylcysteine pre-treatment. These results highlight the involvement of oxidative stress in the toxicity induced by this fungicide, and that free radicals generation plays a key role in the induction of apoptosis probably induced via the mitochondrial pathway.
Asunto(s)
Fungicidas Industriales , Glioma , Plaguicidas , Humanos , Ratas , Animales , Fungicidas Industriales/metabolismo , Plaguicidas/metabolismo , Apoptosis , Daño del ADN , Estrés Oxidativo , Especies Reactivas de Oxígeno/metabolismo , Glioma/metabolismo , CitoesqueletoRESUMEN
Epoxiconazole (EPX), a widely used fungicide for domestic, medical, and industrial applications, could cause neurodegenerative diseases. However, the underling mechanism of neurotoxicity is not well understood. This study aimed to investigate the possible toxic outcomes of Epoxiconzole, a triazole fungicide, on the brain of adult rats in vivo, and in vitro on neural stem cells derived from the subventricular zone of newborn Wistar rats. Our results revealed that oral exposure to EPX at these concentrations (8, 24, 40, 56 mg/kg bw representing respectively NOEL (no observed effect level), NOEL × 3, NOEL × 5, and NOEL × 7) for 28 days caused a considerable generation of oxidative stress in adult rat brain tissue. Furthermore, a signiï¬cant augmentation in lipid peroxidation and protein oxidation has been found. Moreover, it induced an elevation of DNA fragmentation as assessed by the Comet assay. Indeed, EPX administration impaired activities of antioxidant enzymes and inhibited AChE activity. Concomitantly, this pesticide produced histological alterations in the brain of adult rats. Regarding the embryonic neural stem cells, we demonstrated that the treatment by EPX reduced the viability of cells with an IC50 of 10 µM. It also provoked the reduction of cell proliferation, and EPX triggered arrest in G1/S phase. The neurosphere formation and self-renewal capacity was reduced and associated with decreased differentiation. Moreover, EPX induced cytoskeleton disruption as evidenced by immunocytochemical analysis. Our findings also showed that EPX induced apoptosis as evidenced by a loss of mitochondrial transmembrane potential (ΔΨm) and an activation of caspase-3. In addition, EPX promoted ROS production in neural stem cells. Interestingly, the pretreatment of neural stem cells with the N-acetylcysteine (ROS scavenger) attenuated EPX-induced cell death, disruption of neural stem cells properties, ROS generation and apoptosis. Thus, the use of this hazardous material should be restricted and carefully regulated.
Asunto(s)
Células-Madre Neurales , Triazoles , Animales , Apoptosis , Encéfalo , Compuestos Epoxi , Estrés Oxidativo , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno , Triazoles/toxicidadRESUMEN
The widespread use of pesticides is beneficial for food production; however, there are numerous adverse consequences reported in the ecosystem and humans associated with exposure to these contaminants. The pyrethriod bifenthrin (BIF) is utilized for (1) maintenance, growth, and storage of agricultural products; (2) control of internal and external parasites of farm animals; and (3) eradication of insects threatening public health. Numerous data are available regarding environmental and ecological impact of pyrethriods on the central and peripheral nervous systems; however few studies focused on non-target tissues especially in humans. Therefore, the aim of this investigation was to determine the potential cytotoxic effects of BIF on a non-target tissue using human colorectal HCT-116 cells as a model. Data demonstrated that BIF reduced cell viability and disrupted mitochondrial functions which were accompanied by increased reactive oxygen species (ROS) levels indicating the presence of oxidative stress. BIF produced a significant elevation in levels of malondialdehyde (MDA) supporting the role of oxidative stress in pesticide-mediated toxicity. Concomitantly, a fall of mitochondrial transmembrane potential (Δψ), consequently producing perturbation of fluidity as well as excitability of cellular membranes was noted. Our results also indicated that BIF induced a rise in DNA damage as evidenced by the comet assay. An increase in mitogen-activated protein kinases (MAPKs), JNK (N-terminal Kinase), p38, and ERK (extracellular-signal-regulated kinase) suggested an apoptotic effect. Data thus indicated that BIF-induced cytotoxicity in human colorectal HCT-116 cells was associated with oxidative stress, mitochondrial dysfunction, and apoptosis.
Asunto(s)
Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Neoplasias Colorrectales/inducido químicamente , Daño del ADN/efectos de los fármacos , Células HCT116/efectos de los fármacos , Mitocondrias/efectos de los fármacos , Plaguicidas/toxicidad , Piretrinas/toxicidad , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
The di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride industry. Human exposure to this plasticizer is inevitable and contributes to several side effects. In this study, we examined whether DEHP induces apoptosis and oxidative stress in embryonic kidney cells (HEK-293) and whether the nuclear factor E2-related factor 2 (Nrf-2)/heme oxygenase-1 (HO-1) antioxidant pathway is involved in the pathogenesis of this process. We demonstrated that DEHP is cytotoxic to HEK-293 cells. It causes oxidative damage through the generation of free radicals, induces lipid peroxidation, and alters superoxide dismutase and catalase activities. Simultaneously, DEHP treatment decreases the expression and the protein level of Nrf-2 and HO-1. Inhibition of the Nrf-2/HO-1 pathway is related to the mitochondrial pathway of apoptosis. This apoptotic process is characterized by a loss of mitochondrial transmembrane potential (ΔΨm) and upregulation of the expression of caspase-3 mRNA as well as its protein level.
Asunto(s)
Antioxidantes/metabolismo , Apoptosis/efectos de los fármacos , Dietilhexil Ftalato/toxicidad , Hemo-Oxigenasa 1/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Plastificantes/toxicidad , Supervivencia Celular/efectos de los fármacos , Células HEK293 , Humanos , Peroxidación de Lípido/efectos de los fármacos , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Estrés Oxidativo/efectos de los fármacosRESUMEN
A cationic cell-penetrating peptide PEP-NJSM was identified in human virus proteomes by a screening of charge clusters in protein sequences generating Cell-Penetrating Peptides (CPP). PEP-NJSM was selectively active against Gram-positive Staphylococcus epidermidis as antibacterial agent with MIC value of 128⯵M compared to the Gram-negative Pseudomonas aeruginosa strain with MIC value exceeded 512⯵M. The selected peptide exhibited an important anti-biofilm activity even at sub-MIC levels. PEP-NJSM could prevent biofilm formation and increase the mortality of cells inside mature S. epidermidis biofilm. The results demonstrated that PEP-NJSM presented an important anti-adherent activity. It showed a S. epidermidis inhibition of biofilm formation >84% at a concentration of 256⯵M (2 X MIC) and remained active even at a concentration of 4⯵M with 32% of inhibition. The eradication of the established biofilm was observed at a concentration of 256⯵M with 55.7% of biofilm eradication. The peptide was active against mature biofilm even at low concentration of 0.5⯵M with approximately 22.9% of eradication. PEP-NJSM exhibited low hemolytic activity and cytotoxicity against mammalian cells. Our results demonstrate that PEP-NJSM could have a potential role in the treatment of diseases related to Staphylococcus epidermidis infection.
Asunto(s)
Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Péptidos de Penetración Celular/farmacología , Staphylococcus epidermidis/efectos de los fármacos , Secuencia de Aminoácidos , Antibacterianos/química , Adhesión Bacteriana/efectos de los fármacos , Péptidos de Penetración Celular/química , Hemólisis , Humanos , Pruebas de Sensibilidad Microbiana , Conformación MolecularRESUMEN
Sulfated glycosaminoglycans (SGNL) were extracted for the first time from Norway lobster (Nephrops norvegicus) shell. The monosaccharide composition analysed by GC/MS revealed the presence of galacturonic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine. The analysis of SGNL with acetate cellulose electrophoresis in Zn-acetate revealed the presence of heparan sulfate (HS) and dermatan sulfate (DS). SGNL were evaluated for their anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. After 21h incubation, HCT116 cell proliferation was inhibited (p<0.05) between 39.7 and 54.8% at 1.5-7.5mg/mL of SGNL. SGNL don't show hemolytic activity towards bovine erythrocytes and no cytotoxicity against the normal lymphocytes. The antiproliferative efficacy of these lobster glycosaminoglycans were probably related with the higher sulfate content. SGNL demonstrated promising antiproliferative and anticoagulant potential, which may be used as a novel, effective and promising antithrombotic agent.
Asunto(s)
Exoesqueleto/química , Anticoagulantes/farmacología , Colon/patología , Glicosaminoglicanos/aislamiento & purificación , Glicosaminoglicanos/farmacología , Nephropidae/química , Animales , Bovinos , Muerte Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Electroforesis , Células HCT116 , Hemólisis/efectos de los fármacos , Humanos , Tiempo de Tromboplastina Parcial , Tiempo de Protrombina , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The present study was designed to assess the possible protective effects of Quercetin (QUER), a flavonoid with well-known pharmacological effects, against Dichlorvos (DDVP)-induced toxicity in vitro using HCT116 cells. The cytotoxicity was monitored by cell viability, reactive oxygen species (ROS) generation, anti-oxidant enzyme activities, malondialdehyde (MDA) production, and DNA fragmentation. The apoptosis was assessed through the measurement of the mitochondrial transmembrane potential (ΔΨm) and caspase activation. The results indicated that pretreatment of HCT116 cells with QUER, 2 h prior to DDVP exposure, significantly decreased the DDVP-induced cell death, inhibited the ROS generation, modulated the activities of catalase (CAT) and superoxide dismutase (SOD), and reduced the MDA level. The reductions in mitochondrial membrane potential, DNA fragmentation, and caspase activation were also attenuated by QUER. These findings suggest that dietary QUER can protect HCT116 cells against DDVP-induced oxidative stress and apoptosis.