RESUMEN
Two kiwifruit (Actinidia) species with contrasting terpene profiles were compared to understand the regulation of fruit monoterpene production. High rates of terpinolene production in ripe Actinidia arguta fruit were correlated with increasing gene and protein expression of A. arguta terpene synthase1 (AaTPS1) and correlated with an increase in transcript levels of the 2-C-methyl-D-erythritol 4-phosphate pathway enzyme 1-deoxy-D-xylulose-5-phosphate synthase (DXS). Actinidia chinensis terpene synthase1 (AcTPS1) was identified as part of an array of eight tandemly duplicated genes, and AcTPS1 expression and terpene production were observed only at low levels in developing fruit. Transient overexpression of DXS in Nicotiana benthamiana leaves elevated monoterpene synthesis by AaTPS1 more than 100-fold, indicating that DXS is likely to be the key step in regulating 2-C-methyl-D-erythritol 4-phosphate substrate flux in kiwifruit. Comparative promoter analysis identified potential NAC (for no apical meristem [NAM], Arabidopsis transcription activation factor [ATAF], and cup-shaped cotyledon [CUC])-domain transcription factor) and ETHYLENE-INSENSITIVE3-like transcription factor (TF) binding sites in the AaTPS1 promoter, and cloned members of both TF classes were able to activate the AaTPS1 promoter in transient assays. Electrophoretic mobility shift assays showed that AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the AaTPS1 promoter but not the A. chinensis AcTPS1 promoter, where the NAC binding site was mutated. Activation could be restored by reintroducing multiple repeats of the 12-bp NAC core-binding motif. The absence of NAC transcriptional activation in ripe A. chinensis fruit can account for the low accumulation of AcTPS1 transcript, protein, and monoterpene volatiles in this species. These results indicate the importance of NAC TFs in controlling monoterpene production and other traits in ripening fruits.
Asunto(s)
Actinidia/enzimología , Transferasas Alquil y Aril/metabolismo , Regulación de la Expresión Génica de las Plantas , Monoterpenos/metabolismo , Proteínas de Plantas/metabolismo , Actinidia/genética , Actinidia/crecimiento & desarrollo , Transferasas Alquil y Aril/genética , Secuencia de Bases , Eritritol/análogos & derivados , Eritritol/metabolismo , Etilenos/metabolismo , Frutas/enzimología , Frutas/genética , Frutas/crecimiento & desarrollo , Expresión Génica , Datos de Secuencia Molecular , Filogenia , Hojas de la Planta/enzimología , Hojas de la Planta/genética , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas/genética , Alineación de Secuencia , Análisis de Secuencia de ADN , Especificidad de la Especie , Fosfatos de Azúcar/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Transferasas/genética , Transferasas/metabolismoRESUMEN
In the last 30 years the incidence of kiwifruit allergy has increased with the three major allergenic proteins being identified as actinidin, kiwellin, and thaumatin-like protein (TLP). We report wide variation in the levels of actinidin and TLP in 15 kiwifruit varieties from the four most widely cultivated Actinidia species. Acidic and basic isoforms of actinidin were identified in Actinidia deliciosa 'Hayward' and Actinidia arguta 'Hortgem Tahi', while only a basic isoform of actinidin was identified in Actinidia chinensis 'Hort16A'. One isoform each of kiwellin and TLP were identified in ripe fruit. The cysteine protease activity of actinidin correlated with protein levels in all species except A. arguta. Protein modeling suggested that modifications to the S2 binding pocket influenced substrate specificity of the A. arguta enzyme. Our results indicate that care is necessary when extrapolating allergenicity results from single varieties to others within the same and between different Actinidia species.
Asunto(s)
Actinidia/química , Alérgenos/química , Antígenos de Plantas/química , Cisteína Endopeptidasas/química , Frutas/química , Proteínas de Plantas/química , Actinidia/inmunología , Alérgenos/inmunología , Secuencia de Aminoácidos , Antígenos de Plantas/inmunología , Western Blotting , Cromatografía Liquida , Cisteína Endopeptidasas/inmunología , Electroforesis en Gel de Poliacrilamida , Espectrometría de Masas , Datos de Secuencia Molecular , Proteínas de Plantas/inmunología , Conformación ProteicaRESUMEN
Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putative terpene synthase (TPS) genes, of which only 10 are predicted to be functional. This low number of predicted functional TPS genes compared with other plant species was supported by the identification of only eight potentially functional TPS enzymes in apple 'Royal Gala' expressed sequence tag databases, including the previously characterized apple (E,E)-α-farnesene synthase. In planta functional characterization of these TPS enzymes showed that they could account for the majority of terpene volatiles produced in cv Royal Gala, including the sesquiterpenes germacrene-D and (E)-ß-caryophyllene, the monoterpenes linalool and α-pinene, and the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene. Relative expression analysis of the TPS genes indicated that floral and vegetative tissues were the primary sites of terpene production in cv Royal Gala. However, production of cv Royal Gala floral-specific terpenes and TPS genes was observed in the fruit of some heritage apple cultivars. Our results suggest that the apple TPS gene family has been shaped by a combination of ancestral and more recent genome-wide duplication events. The relatively small number of functional enzymes suggests that the remaining terpenes produced in floral and vegetative and fruit tissues are maintained under a positive selective pressure, while the small number of terpenes found in the fruit of modern cultivars may be related to commercial breeding strategies.
Asunto(s)
Transferasas Alquil y Aril/genética , Genómica/métodos , Malus/genética , Familia de Multigenes , Proteínas de Plantas/genética , Terpenos/metabolismo , Monoterpenos Acíclicos , Transferasas Alquil y Aril/clasificación , Transferasas Alquil y Aril/metabolismo , Secuencia de Bases , Monoterpenos Bicíclicos , Flores/genética , Flores/metabolismo , Frutas/genética , Frutas/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Perfilación de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Malus/clasificación , Malus/metabolismo , Datos de Secuencia Molecular , Monoterpenos/química , Monoterpenos/metabolismo , Filogenia , Proteínas de Plantas/clasificación , Proteínas de Plantas/metabolismo , Sesquiterpenos Policíclicos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Ácido Nucleico , Sesquiterpenos/química , Sesquiterpenos/metabolismo , Especificidad de la Especie , Terpenos/química , Compuestos Orgánicos Volátiles/química , Compuestos Orgánicos Volátiles/metabolismo , VolatilizaciónRESUMEN
In vitro-based analyses of monoterpene synthase (mono-TPS) enzymes have led to a wealth of knowledge regarding their catalytic behavior, the mechanistic principles governing their product specificity, and the molecular basis for their evolution. However, the efficient production of active enzymes in Escherichia coli or yeast can be challenging. Agrobacterium-mediated transient expression in tobacco leaves is increasingly being used as a viable alternative to in vitro-based approaches for the production and functional analysis of a wide range of plant proteins. Transient expression is well suited for qualitative and semiquantitative analyses of mono-TPS enzyme product specificity and, in conjunction with standard volatile analysis techniques, provides an efficient tool for screening mono-TPS function in planta. The primary advantages of this system for mono-TPS analysis are that both mono-TPS genomic clones and cDNAs can be cloned directly into plant expression vectors without modification and expressed enzymes can be analyzed without the need for purification or endogenous precursor addition. Here, we describe a simple and cost-effective method for the in planta functional analysis of plant mono-TPS enzymes. This method can accommodate both the analysis of single genes and the scaling for more high-throughput functional screening of mono-TPS gene families or mutant libraries.
Asunto(s)
Regulación Enzimológica de la Expresión Génica , Genes de Plantas , Liasas Intramoleculares/metabolismo , Nicotiana/enzimología , Agrobacterium tumefaciens/genética , Agrobacterium tumefaciens/metabolismo , Clonación Molecular , Vectores Genéticos/genética , Vectores Genéticos/metabolismo , Ensayos Analíticos de Alto Rendimiento , Hidroliasas/genética , Hidroliasas/metabolismo , Liasas Intramoleculares/genética , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sensibilidad y Especificidad , Especificidad por Sustrato , Nicotiana/genética , Transformación GenéticaRESUMEN
Flowers of the kiwifruit species Actinidia chinensis produce a mixture of sesquiterpenes derived from farnesyl diphosphate (FDP) and monoterpenes derived from geranyl diphosphate (GDP). The tertiary sesquiterpene alcohol (E)-nerolidol was the major emitted volatile detected by headspace analysis. Contrastingly, in solvent extracts of the flowers, unusually high amounts of (E,E)-farnesol were observed, as well as lesser amounts of (E)-nerolidol, various farnesol and farnesal isomers, and linalool. Using a genomics-based approach, a single gene (AcNES1) was identified in an A. chinensis expressed sequence tag library that had significant homology to known floral terpene synthase enzymes. In vitro characterization of recombinant AcNES1 revealed it was an enzyme that could catalyse the conversion of FDP and GDP to the respective (E)-nerolidol and linalool terpene alcohols. Enantiomeric analysis of both AcNES1 products in vitro and floral terpenes in planta showed that (S)-(E)-nerolidol was the predominant enantiomer. Real-time PCR analysis indicated peak expression of AcNES1 correlated with peak (E)-nerolidol, but not linalool accumulation in flowers. This result, together with subcellular protein localization to the cytoplasm, indicated that AcNES1 was acting as a (S)-(E)-nerolidol synthase in A. chinensis flowers. The synthesis of high (E,E)-farnesol levels appears to compete for the available pool of FDP utilized by AcNES1 for sesquiterpene biosynthesis and hence strongly influences the accumulation and emission of (E)-nerolidol in A. chinensis flowers.
Asunto(s)
Actinidia/enzimología , Farnesol/metabolismo , Flores/enzimología , Proteínas de Plantas/metabolismo , Sesquiterpenos/metabolismo , Actinidia/genética , Actinidia/metabolismo , Monoterpenos Acíclicos , Transferasas Alquil y Aril/genética , Transferasas Alquil y Aril/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Secuencia de Bases , Difosfatos/metabolismo , Diterpenos/metabolismo , Farnesol/análisis , Flores/genética , Flores/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Cinética , Datos de Secuencia Molecular , Monoterpenos/análisis , Monoterpenos/metabolismo , Aceites Volátiles/análisis , Aceites Volátiles/metabolismo , Filogenia , Hojas de la Planta/enzimología , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Proteínas de Plantas/genética , Fosfatos de Poliisoprenilo/metabolismo , Proteínas Recombinantes , Análisis de Secuencia de ADN , Sesquiterpenos/análisis , Especificidad por Sustrato , Nicotiana/genética , Nicotiana/metabolismoRESUMEN
Kiwifruit vines rely on bees for pollen transfer between spatially separated male and female individuals and require synchronized flowering to ensure pollination. Volatile terpene compounds, which are important cues for insect pollinator attraction, were studied by dynamic headspace sampling in the major green-fleshed kiwifruit (Actinidia deliciosa) cultivar 'Hayward' and its male pollinator 'Chieftain'. Terpene volatile levels showed a profile dominated by the sesquiterpenes alpha-farnesene and germacrene D. These two compounds were emitted by all floral tissues and could be observed throughout the day, with lower levels at night. The monoterpene (E)-beta-ocimene was also detected in flowers but was emitted predominantly during the day and only from petal tissue. Using a functional genomics approach, two terpene synthase (TPS) genes were isolated from a 'Hayward' petal EST library. Bacterial expression and transient in planta data combined with analysis by enantioselective gas chromatography revealed that one TPS produced primarily (E,E)-alpha-farnesene and small amounts of (E)-beta-ocimene, whereas the second TPS produced primarily (+)-germacrene D. Subcellular localization using GFP fusions showed that both enzymes were localized in the cytoplasm, the site for sesquiterpene production. Real-time PCR analysis revealed that both TPS genes were expressed in the same tissues and at the same times as the corresponding floral volatiles. The results indicate that two genes can account for the major floral sesquiterpene volatiles observed in both male and female A. deliciosa flowers.