RESUMEN
The cytotoxicity of glass ionomer cements (GICs) was investigated using a novel, cost-effective, easy-to-perform and standardized test. GIC rings were made using in-house designed, custom-made moulds under sterile conditions; 10 with Fuji Equia and 10 with Fuji Triage capsules, placed in direct contact with primary human gingival fibroblasts (HGF) and immortalized human fibroblasts (HFF1). On day 1, 4, 14 and 21, an AlamarBlue® (resazurin) assay was completed towards determining the effects of the GICs on metabolic activities of the cells, whilst cell morphology was examined by light microscopy. The influence of the compounds released from the GIC rings on cell physiological effects (viability, proliferation and adhesion) during 24â¯h incubation was further investigated by impedimetry. Result trends obtained from this battery of techniques were complementary. At 100â¯v/v% concentration, the released compounds from Equia were strongly cytotoxic, while at lower concentration (0, 4, 20â¯v/v%) they were not cytotoxic. In contrast, Triage elicited only slightly transient cytotoxicity. The method proposed has been proved as being efficient, reliable and reproducible and may be useful in quick testing of the cytotoxicity of similar biomaterials by using an immortalized cell line.
Asunto(s)
Materiales Biocompatibles/toxicidad , Fibroblastos/efectos de los fármacos , Cementos de Ionómero Vítreo/toxicidad , Adhesión Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Fibroblastos/fisiología , Humanos , Ensayo de Materiales/métodosRESUMEN
There is no effective treatment for the loss of functional salivary tissue after irradiation for head and neck cancer or the autoimmune disease Sjögren's syndrome. One possible approach is the regeneration of salivary glands from stem cells. The present study aimed to investigate whether small pieces of human submandiblar gland tissue contain elements necessary for the reconstruction of salivary rudiments in vitro via acinar and ductal cell differentiation. Primary submandibular gland (primary total human salivary gland; PTHSG) cells were isolated from human tissue and cultured in vitro using a new method in which single cells form an expanding epithelial monolayer on plastic substrates. Differentiation, morphology, number, and organization of these cells were then followed on basement membrane extract (BME) using RNA quantitation (amylase, claudin-1 (CLN1), CLN3, kallikrein, vimentin), immunohistochemistry (amylase and occludin), viability assay, and videomicroscopy. On the surface of BME, PTHSG cells formed acinotubular structures within 24 h, did not proliferate, and stained for amylase. In cultures derived from half of the donors, the acinar markers amylase and CLN3 were upregulated. The PTHSG culture model suggests that human salivary gland may be capable of regeneration via reorganization and differentiation and that basement membrane components play a crucial role in the morphological and functional differentiation of salivary cells.
Asunto(s)
Membrana Basal/metabolismo , Diferenciación Celular/fisiología , Proliferación Celular , Glándula Submandibular/citología , Amilasas/genética , Amilasas/metabolismo , Diferenciación Celular/genética , Supervivencia Celular , Claudina-1 , Humanos , Inmunohistoquímica , Calicreínas/genética , Calicreínas/metabolismo , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Ocludina , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Glándula Submandibular/metabolismo , Vimentina/genética , Vimentina/metabolismoRESUMEN
INTRODUCTION: In salivary glands, fluid transport is thought to be driven osmotically in response to transepithelial salt gradients. According to the classical two-stage hypothesis of salivary secretion, an isotonic primary fluid is generated by the acinar cells and the fluid is subsequently modified by solute reabsorption and secretion as it passes along the ductal system resulted in the final, hypotonic solution. AIM: Very little is known about the molecular and functional nature of the transporters involved in salivary secretion, especially in human salivary glands. Therefore a systematic investigation of membrane transporters expressed also in the kidney, has been undertaken in healthy human salivary glands. METHODS: RT-PCR and immunohistochemical analysis of different membrane transport proteins was used in rat and human salivary glands. RESULTS: Clear evidence for the expression of aquaporin water channels in human salivary glands was found. AQP1 in the myoepithelial cells, AQP3 in the basolateral, AQP5 in the apical membrane of the acini is localized. The electroneutral NBC3 Na(+)-HCO3(-)-cotransporter is present in the apical membrane of the serous acini and of the ducts, while the NBCn1 only in the basolateral membrane of the striated duct is localized. The NHE1 Na+/H+ exchanger is present in the basolateral membrane of the acini and ducts. The vacuolar H(+)-ATPase is localized apically in the ducts, except for the intercalated duct. CONCLUSION: Aquaporin water channels are likely to be involved in water secretion. The NBC3 and NBCn1 electroneutral Na(+)-HCO3(-)-cotransporters, the NHE1 Na+/H+ exchanger and the vacuolar H(+)-ATPase may play an important role in the pH regulation of salivary acinar and duct cells.
Asunto(s)
Electrólitos/análisis , Saliva/química , Saliva/metabolismo , Glándulas Salivales/química , Animales , Acuaporinas/análisis , Proteínas de Transporte de Catión/análisis , Humanos , Concentración de Iones de Hidrógeno , Inmunohistoquímica , Proteínas de Transporte de Membrana/análisis , ATPasas de Translocación de Protón/análisis , Ratas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Simportadores de Sodio-Bicarbonato/análisis , Intercambiador 1 de Sodio-Hidrógeno , Intercambiadores de Sodio-Hidrógeno/análisis , Agua/químicaRESUMEN
BACKGROUND INFORMATION: Phenotype analysis has demonstrated that AQP3 (aquaporin 3) null mice are polyuric and manifest a urinary concentration defect. In the present study, we report that deletion of AQP3 is also associated with an increased urinary sodium excretion. To investigate further the mechanism of the decreased urinary concentration and significant natriuresis, we examined the segmental and subcellular localization of collecting duct AQPs [AQP2, p-AQP2 (phosphorylated AQP2), AQP3 and AQP4], ENaC (epithelial sodium channel) subunits and Na,K-ATPase by immunoperoxidase and immunofluorescence microscopy in AQP3 null (-/-), heterozygous (+/-) mice, wild-type and unrelated strain of normal mice. RESULTS: The present study confirms that AQP3 null mice exhibit severe polyuria and polydipsia and demonstrated that they exhibit increased urinary sodium excretion. In AQP3 null mice, there is a marked down-regulation of AQP2 and p-AQP2 both in CNT (connecting tubule) and CCD (cortical collecting duct). Moreover, AQP4 is virtually absent from CNT and CCD in AQP3 null mice. Basolateral AQP2 was virtually absent from AQP3 null mice and normal mice in contrast with rat. Thus the above results demonstrate that no basolateral AQPs are expressed in CNT and CCD of AQP3 null mice. However, in the medullary-collecting ducts, there is no difference in the expression levels and subcellular localization of AQP2, p-AQP2 and AQP4 between AQP3 +/- and AQP3 null mice. Moreover, a striking decrease in the immunolabelling of the alpha1 subunit of Na,K-ATPase was observed in CCD in AQP3 null mice, whereas a medullary-collecting duct exhibited normal labelling. Immunolabelling of all the ENaC subunits in the collecting duct was comparable between the two groups. CONCLUSIONS: The results improve the possibility that the severe urinary concentrating defect in AQP3 null mice may in part be caused by the decreased expression of AQP2, p-AQP2 and AQP4 in CNT and CCD, whereas the increased urinary sodium excretion may in part be accounted for by Na,K-ATPase in CCD in AQP3 null mice.
Asunto(s)
Acuaporinas/biosíntesis , Acuaporinas/deficiencia , Regulación Enzimológica de la Expresión Génica/genética , Túbulos Renales Colectores/enzimología , ATPasa Intercambiadora de Sodio-Potasio/biosíntesis , Animales , Acuaporina 2 , Acuaporina 3 , Acuaporina 4 , Acuaporinas/genética , Túbulos Renales Colectores/citología , Ratones , Ratones Mutantes , Natriuresis/genética , ATPasa Intercambiadora de Sodio-Potasio/genética , OrinaRESUMEN
In vitro studies of cultured salivary gland cells and gland slices have indicated that there may be regulated translocation of aquaporin (AQP)-5 between the apical plasma membrane and intracellular compartments of the secretory cells. However, it remains unknown whether AQP-5 in salivary glands is subject to regulated trafficking in vivo. To examine this possibility, we have investigated the subcellular localization of AQP-5 in rat parotid and submandibular glands fixed in vivo under conditions of stimulated or inhibited salivary secretion. Immunofluorescence and immunoelectron microscopy was used to determine the subcellular distribution of AQP-5 in control conditions following the stimulation of secretion with pilocarpine (a muscarinic agonist) or epinephrine (an alpha-adrenoceptor agonist) or during inhibition of basal secretion with atropine (a muscarinic antagonist) or phentolamine (an alpha-adrenoceptor antagonist). Under control conditions, >90% of AQP-5 was associated with the apical plasma membrane of acinar and intercalated duct cells, with only rare gold particles associated with intracellular membrane domains. Pilocarpine treatment dramatically increased saliva production but had no discernible effect on AQP-5 distribution. However, the increased salivary secretion was associated with luminal dilation and the appearance of a markedly punctate AQP-5 labeling pattern due to clustering of AQP-5 at the microvilli (especially evident in the parotid gland) after 10 min of drug injection. No changes in the subcellular localization of AQP-5 were seen in response to epinephrine, atropine, or phentolamine treatment compared with control tissues. Thus AQP-5 is localized predominantly in the apical plasma membrane under control conditions, and neither the onset nor the cessation of secretion is associated in vivo with any significant short-term translocation of AQP-5 between intracellular structures and the apical plasma membrane.