Differential effects of two prevalent environmental pollutants on host-pathogen dynamics.

Chemical pollutants are a major factor implicated in freshwater habitat degradation and species loss. Microplastics and glyphosate-based herbicides are prevalent pollutants with known detrimental effects on animal welfare but our understanding of their impacts on infection dynamics are limited. Within freshwater vertebrates, glyphosate formulations reduce fish tolerance to infections, but the effects of microplastic consumption on disease tolerance have thus far not been assessed. Here, we investigated how microplastic (polypropylene) and the commercial glyphosate-based herbicide, Roundup®, impact fish tolerance to infectious disease and mortality utilising a model fish host-pathogen system. For uninfected fish, microplastic and Roundup had contrasting impacts on mortality as individual stressors, with microplastic increasing and Roundup decreasing mortality compared with control fish not exposed to pollutants. Concerningly, microplastic and Roundup combined had a strong interactive reversal effect by significantly increasing host mortality for uninfected fish (73% mortality). For infected fish, the individual stressors also had contrasting effects on mortality, with microplastic consumption not significantly affecting mortality and Roundup increasing mortality to 55%. When combined, these two pollutants had a moderate interactive synergistic effect on mortality levels of infected fish (53% mortality). Both microplastic and Roundup individually had significant and contrasting impacts on pathogen metrics with microplastic consumption resulting in fish maintaining infections for significantly longer and Roundup significantly reducing pathogen burdens. When combined, the two pollutants had a largely additive effect in reducing pathogen burdens. This study is the first to reveal that microplastic and Roundup individually and interactively impact host-pathogen dynamics and can prove fatal to fish.

Growth yields in bacterial denitrification and nitrate ammonification.

Denitrification and nitrate ammonification are considered the highest-energy-yielding respiration systems in anoxic environments after oxygen has been consumed. The corresponding free energy changes are 7 and 35% lower than that of aerobic respiration, respectively. Growth yield determinations with pure cultures of Paracoccus denitrificans and Pseudomonas stutzeri revealed that far less energy is converted via ATP into cell mass than expected from the above calculations. Denitrification with formate or hydrogen as electron donor yielded about 2.4 to 3.0 g dry matter per mol formate or hydrogen and 15 to 18 g dry matter per mol acetate. Similar yields with acetate were obtained with Pseudomonas stutzeri. Wolinella succinogenes and Sulfurospirillum deleyianum, which reduce nitrate to ammonia, both exhibited similar yield values with formate or H2 plus nitrate. The results indicate that ATP synthesis in denitrification is far lower than expected from the free energy changes and even lower than in nitrate ammonification. The results are discussed against the background of our present understanding of electron flow in denitrification and with respect to the importance of denitrification and nitrate ammonification in the environment.