RESUMEN
The glucagon receptor was cloned from cynolomologous monkey. A frame-shift mutation at the 3' end of the monkey transcript results in a C-terminal extension of 14 amino acids. This extension is not observed in either the human or rodent glucagon receptors. Monkey glucagon receptor was expressed in CHO cells, either with (mkGCGR) or without (mkGCGRDelta14) the 14-amino acid C-terminal extension to approximate the human receptor. Both forms of the monkey receptor bound glucagon with similar affinity and showed glucagon-stimulated cAMP production, however the full-length form of the monkey receptor (mkGCGR) was less sensitive to glucagon in its ability to stimulate cAMP than the shortened form (mkGCGRDelta14). PCR of genomic DNA from baboon and rhesus monkeys suggests that they express a form of the receptor similar to that of cynomologous monkey, while in chimpanzee, the receptor is similar to the human form.
Asunto(s)
Haplorrinos/genética , Receptores de Glucagón/genética , Receptores de Glucagón/metabolismo , Animales , Secuencia de Bases , Células CHO , Clonación Molecular , Cricetinae , AMP Cíclico/metabolismo , ADN Complementario/genética , Glucagón/metabolismo , Humanos , Datos de Secuencia Molecular , ARN/genética , Receptores de Glucagón/química , Alineación de Secuencia , Homología de Secuencia de Ácido NucleicoRESUMEN
Biaryl amides derived from a reported series of ureas 1 were evaluated and found to be potent human glucagon receptor antagonists. The benzofuran analogue 6i was administered in Sprague-Dawley rats and blocked the effects of an exogenous glucagon challenge.