RESUMEN
Factors regulating larval growth and determinants of adult body size are described for several holometabolous insects, but less is known about brain size scaling through development. Here we use the isotropic fractionation ("brain soup") method to estimate the number of brain cells and cell density for the whitelined sphinx moth (Lepidoptera: Hyles lineata) from the first instar through the adult stage. We measure mass and brain cell number and find that, during the larval stages, body mass shows an exponential relationship with head width, while the total number of brain cells increases asymptotically. Larval brain cell number increases by a factor of ten from nearly 8000 in the first instar to over 80,000 in the fifth instar. Brain cell number increases by another factor of 10 during metamorphosis, with the adult brain containing more than 900,000 cells. This is similar to increases during development in the vinegar fly (Drosophila melanogaster) and the black soldier fly (Hermetia illucens). The adult brain falls slightly below the brain-to-body allometry for wasps and bees but is comparable in the number of cells per unit brain mass, indicating a general conservation of brain cell density across these divergent lineages.
Asunto(s)
Mariposas Nocturnas , Avispas , Abejas , Animales , Drosophila melanogaster , Tamaño de los Órganos , Larva , Recuento de CélulasRESUMEN
The ongoing decline of bee populations and its impact on food security demands integrating multiple strategies. Sublethal impairments associated with exposure to insecticides, affecting the individual and the colony levels, have led to insecticide moratoria and bans. However, legislation alone is not sufficient and remains a temporary solution to an evolving market of insecticides. Here, we asked whether bees can be prophylactically protected against sublethal cognitive effects of two major neurotoxic insecticides, imidacloprid and fipronil, with different mechanisms of action. We evaluated the protective effect of the prophylactic administration of the flavonoid rutin, a secondary plant metabolite, present in nectar and pollen, and known for its neuroprotective properties. Following controlled or ad libitum administration of rutin, foragers of the North American bumble bee Bombus impatiens received oral administration of the insecticides at sublethal realistic dosages. Learning acquisition, memory retention and decision speed were evaluated using olfactory absolute conditioning of the proboscis extension response. We show that the insecticides primarily impair acquisition but not retention or speed of the conditioned proboscis extension response. We further show that the administration of the flavonoid rutin successfully protects the bees against impairments produced by acute and chronic administration of insecticides. Our results suggest a new avenue for the protection of bees against sublethal cognitive effects of insecticides.
Asunto(s)
Disfunción Cognitiva , Insecticidas , Animales , Abejas , Flavonoides , Imidazoles/toxicidad , Insecticidas/toxicidad , Neonicotinoides/toxicidad , Nitrocompuestos , Pirazoles , Rutina/farmacologíaRESUMEN
Many comparative neurobiological studies seek to connect sensory or behavioural attributes across taxa with differences in their brain composition. Recent studies in vertebrates suggest cell number and density may be better correlated with behavioural ability than brain mass or volume, but few estimates of such figures exist for insects. Here, we use the isotropic fractionator (IF) method to estimate total brain cell numbers for 32 species of Hymenoptera spanning seven subfamilies. We find estimates from using this method are comparable to traditional, whole-brain cell counts of two species and to published estimates from established stereological methods. We present allometric scaling relationships between body and brain mass, brain mass and nuclei number, and body mass and cell density and find that ants stand out from bees and wasps as having particularly small brains by measures of mass and cell number. We find that Hymenoptera follow the general trend of smaller animals having proportionally larger brains. Smaller Hymenoptera also feature higher brain cell densities than the larger ones, as is the case in most vertebrates, but in contrast with primates, in which neuron density remains rather constant across changes in brain mass. Overall, our findings establish the IF as a useful method for comparative studies of brain size evolution in insects.
Asunto(s)
Hormigas , Himenópteros , Avispas , Animales , Abejas , Encéfalo , Recuento de Células , PrimatesRESUMEN
Whip spiders (Amblypygi) are known for their nocturnal navigational abilities, which rely on chemosensory and tactile cues and, to a lesser degree, on vision. Unlike true spiders, the first pair of legs in whip spiders is modified into extraordinarily long sensory organs (antenniform legs) covered with thousands of mechanosensory, olfactory, and gustatory sensilla. Olfactory neurons send their axons through the leg nerve into the corresponding neuromere of the central nervous system, where they terminate on a particularly large number (about 460) of primary olfactory glomeruli, suggesting an advanced sense of smell. From the primary glomeruli, olfactory projection neurons ascend to the brain and terminate in the mushroom body calyx on a set of secondary olfactory glomeruli, a feature that is not known from olfactory pathways of other animals. Another part of the calyx receives visual input from the secondary visual neuropil (the medulla). This calyx region is composed of much smaller glomeruli ("microglomeruli"). The bimodal input and the exceptional size of their mushroom bodies may support the navigational capabilities of whip spiders. In addition to input to the mushroom body, we describe other general anatomical features of the whip spiders' central nervous system.
Asunto(s)
Cuerpos Pedunculados/citología , Vías Olfatorias/citología , Escorpiones/anatomía & histología , Vías Visuales/citología , Animales , Sistema Nervioso Central/anatomía & histología , Sistema Nervioso Central/citología , Vías Olfatorias/fisiología , Escorpiones/fisiología , Vías Visuales/fisiologíaRESUMEN
Similar to animal communication displays, flowers emit complex signals that attract pollinators. Signal complexity could lead to higher cognitive load for pollinators, impairing performance, or might benefit them by facilitating learning, memory and decision making. Here, we evaluated learning and memory in foragers of the bumble bee Bombus impatiens trained to simple (unimodal) versus complex (bimodal) signals under restrained conditions. Use of a proboscis extension response protocol enabled us to control the timing and duration of stimuli presented during absolute and differential learning tasks. Overall, we observed broad variation in performance under the two conditions, with bees trained to compound bimodal signals learning and remembering as well as, better than or more poorly than bees trained to unimodal signals. Interestingly, the outcome of training was affected by the specific colour-odour combination. Among unimodal stimuli, the performance with odour stimuli was higher than with colour stimuli, suggesting that olfactory signals played a more significant role in the compound bimodal condition. This was supported by the fact that after 24â h, most bimodal-treatment bees responded to odour but not visual stimuli. We did not observe differences in latency of response, suggesting that signal composition affected decision accuracy, not speed. We conclude that restrained bumble bee workers exhibit broad variation of responses to bimodal stimuli and that components of the bimodal signal may not be used equivalently. The analysis of bee performance under restrained conditions enables accurate control of the multimodal stimuli provided to individuals and to study the interaction of individual components within a compound.
Asunto(s)
Aprendizaje , Olfato , Animales , Abejas , Flores , OdorantesRESUMEN
Superorganisms represent a unique level of biological organization in which the phenotype of the reproductive unit, the colony, results from traits expressed at the level of individual workers. Because body size scaling has important consequences for cell diversity and system complexity in solitary organisms, colony size is a trait of particular interest in superorganism evolution. In some instances, division of labor and worker polymorphism scale with colony size, but in general little is known about how colony size drives differences in individual-level behavior or neural traits. Ants represent the greatest diversity of superorganisms and provide a manner of natural experiment to test trends in trait evolution across multiple instances of colony size expansion. In this study, we control for environmental differences and worker size polymorphism to test if colony size correlates with measures of foraging behavior and brain size in dolichoderine ants. We present data from 3 species ranked by colony size. Our results suggest colony size correlates with measures of exploratory behavior and brain investment, with small-colony ants showing higher exploratory drive and faster exploration rate than the larger colony species, and greater relative investment in the primary olfactory brain region, the antennal lobe, than the larger colony species.
Asunto(s)
Hormigas/fisiología , Conducta Apetitiva/fisiología , Conducta Animal/fisiología , Encéfalo/anatomía & histología , Conducta Exploratoria/fisiología , Tamaño de los Órganos/fisiología , Conducta Social , AnimalesRESUMEN
Sociality is classified as one of the major transitions in the evolution of complexity and much effort has been dedicated to understanding what traits predispose lineages to sociality. Conversely, studies addressing the role of sociality in brain evolution (e.g., the social brain hypothesis) have not focused on particular traits and instead relied largely on measurements of relative brain composition. Hymenoptera range from solitary to advanced social species, providing enticing comparisons for studying sociality and neural trait evolution. Here we argue that measuring the role of sociality in brain evolution will benefit from attending to recent advances in neuroethology and adopting existing phylogenetic comparative methods employed in analysis of non-neural traits. Such analyses should rely on traits we expect to vary at the taxonomic level used in comparative analyses and include phylogenetic structure. We outline the limits of brain size and volumetric interpretation and advocate closer attention to trait stability and plasticity at different levels of organization. We propose neural traits measured at the cellular, circuit, and molecular levels will serve as more robust variables for evolutionary analyses. We include examples of particular traits and specific clades that are well-suited to answer questions about the role of sociality in nervous system evolution.
Asunto(s)
Conducta Animal , Evolución Biológica , Encéfalo/fisiología , Insectos/fisiología , Vías Nerviosas/fisiología , Conducta Social , Animales , Encéfalo/crecimiento & desarrollo , Insectos/crecimiento & desarrollo , Vías Nerviosas/crecimiento & desarrollo , Plasticidad Neuronal , Tamaño de los Órganos , Especificidad de la Especie , Transmisión SinápticaRESUMEN
The neural mechanisms underlying behavioral variation among individuals are not well understood. Differences among individuals in sensory sensitivity could limit the environmental stimuli to which an individual is capable of responding and have, indeed, been shown to relate to behavioral differences in different species. Here, we show that ant workers in Temnothorax rugatulus differ considerably in the number of antennal sensory structures, or sensilla (by 45% in density and over 100% in estimated total number). A larger quantity of sensilla may reflect a larger quantity of underlying sensory neurons. This would increase the probability that a given set of neurons in the antenna detects an environmental stimulus and becomes excited, thereby eliciting the expression of a behavior downstream at lower stimulus levels than an individual with comparatively fewer sensilla. Individual differences in antennal sensilla density, however, did not predict worker activity level or performance of any task, suggesting either that variation in sensilla density does not, in fact, reflect variation in sensory sensitivity or that individual sensory response thresholds to task-associated stimuli do not determine task allocation as is commonly assumed, at least in this social insect. More broadly, our finding that even closely related individuals can differ strongly in peripheral sensory organ elaboration suggests that such variation in sensory organs could underlie other cases of intraspecific behavioral variation.
Asunto(s)
Hormigas/fisiología , Conducta Animal/fisiología , Sensilos/fisiología , Animales , Microscopía Electrónica de Rastreo , Neuronas/fisiologíaRESUMEN
Trap-jaw ants are characterized by high-speed mandibles used for prey capture and defense. Power-amplified mandibles have independently evolved at least four times among ants, with each lineage using different structures as a latch, spring and trigger. We examined two species from the genus Myrmoteras (subfamily Formicinae), whose morphology is unique among trap-jaw ant lineages, and describe the performance characteristics, spring-loading mechanism and neuronal control of Myrmoteras strikes. Like other trap-jaw ants, Myrmoteras latch their jaws open while the large closer muscle loads potential energy in a spring. The latch differs from other lineages and is likely formed by the co-contraction of the mandible opener and closer muscles. The cuticle of the posterior margin of the head serves as a spring, and is deformed by approximately 6% prior to a strike. The mandibles are likely unlatched by a subgroup of closer muscle fibers with particularly short sarcomeres. These fast fibers are controlled by two large motor neurons whose dendrites overlap with terminals of large sensory neurons originating from labral trigger hairs. Upon stimulation of the trigger hairs, the mandibles shut in as little as 0.5â ms and at peak velocities that are comparable with other trap-jaw ants, but with much slower acceleration. The estimated power output of the mandible strike (21â kWâ kg-1) confirms that Myrmoteras jaws are indeed power amplified. However, the power output of Myrmoteras mandibles is significantly lower than distantly related trap-jaw ants using different spring-loading mechanisms, indicating a relationship between power-amplification mechanism and performance.
Asunto(s)
Hormigas/anatomía & histología , Hormigas/fisiología , Animales , Evolución Biológica , Fenómenos Biomecánicos , Mandíbula/anatomía & histología , Mandíbula/fisiologíaRESUMEN
The metabolic expense of producing and operating neural tissue required for adaptive behaviour is considered a significant selective force in brain evolution. In primates, brain size correlates positively with group size, presumably owing to the greater cognitive demands of complex social relationships in large societies. Social complexity in eusocial insects is also associated with large groups, as well as collective intelligence and division of labour among sterile workers. However, superorganism phenotypes may lower cognitive demands on behaviourally specialized workers resulting in selection for decreased brain size and/or energetic costs of brain metabolism. To test this hypothesis, we compared brain investment patterns and cytochrome oxidase (COX) activity, a proxy for ATP usage, in two ant species contrasting in social organization. Socially complex Oecophylla smaragdina workers had larger brain size and relative investment in the mushroom bodies (MBs)-higher order sensory processing compartments-than the more socially basic Formica subsericea workers. Oecophylla smaragdina workers, however, had reduced COX activity in the MBs. Our results suggest that as in primates, ant group size is associated with large brain size. The elevated costs of investment in metabolically expensive brain tissue in the socially complex O. smaragdina, however, appear to be offset by decreased energetic costs.
Asunto(s)
Hormigas/fisiología , Encéfalo/fisiología , Conducta Social , Animales , Cognición , Complejo IV de Transporte de Electrones/fisiología , Metabolismo Energético , Cuerpos Pedunculados/fisiología , Tamaño de los ÓrganosRESUMEN
Navigation is an ideal behavioral model for the study of sensory system integration and the neural substrates associated with complex behavior. For this broader purpose, however, it may be profitable to develop new model systems that are both tractable and sufficiently complex to ensure that information derived from a single sensory modality and path integration are inadequate to locate a goal. Here, we discuss some recent discoveries related to navigation by amblypygids, nocturnal arachnids that inhabit the tropics and sub-tropics. Nocturnal displacement experiments under the cover of a tropical rainforest reveal that these animals possess navigational abilities that are reminiscent, albeit on a smaller spatial scale, of true-navigating vertebrates. Specialized legs, called antenniform legs, which possess hundreds of olfactory and tactile sensory hairs, and vision appear to be involved. These animals also have enormous mushroom bodies, higher-order brain regions that, in insects, integrate contextual cues and may be involved in spatial memory. In amblypygids, the complexity of a nocturnal rainforest may impose navigational challenges that favor the integration of information derived from multimodal cues. Moreover, the movement of these animals is easily studied in the laboratory and putative neural integration sites of sensory information can be manipulated. Thus, amblypygids could serve as model organisms for the discovery of neural substrates associated with a unique and potentially sophisticated navigational capability. The diversity of habitats in which amblypygids are found also offers an opportunity for comparative studies of sensory integration and ecological selection pressures on navigation mechanisms.
RESUMEN
Group size in both multicellular organisms and animal societies can correlate with the degree of division of labour. For ants, the task specialization hypothesis (TSH) proposes that increased behavioural specialization enabled by larger group size corresponds to anatomical specialization of worker brains. Alternatively, the social brain hypothesis proposes that increased levels of social stimuli in larger colonies lead to enlarged brain regions in all workers, regardless of their task specialization. We tested these hypotheses in acacia ants (Pseudomyrmex spinicola), which exhibit behavioural but not morphological task specialization. In wild colonies, we marked, followed and tested ant workers involved in foraging tasks on the leaves (leaf-ants) and in defensive tasks on the host tree trunk (trunk-ants). Task specialization increased with colony size, especially in defensive tasks. The relationship between colony size and brain region volume was task-dependent, supporting the TSH. Specifically, as colony size increased, the relative size of regions within the mushroom bodies of the brain decreased in trunk-ants but increased in leaf-ants; those regions play important roles in learning and memory. Our findings suggest that workers specialized in defence may have reduced learning abilities relative to leaf-ants; these inferences remain to be tested. In societies with monomorphic workers, brain polymorphism enhanced by group size could be a mechanism by which division of labour is achieved.
Asunto(s)
Hormigas/anatomía & histología , Hormigas/fisiología , Cuerpos Pedunculados/anatomía & histología , Animales , Encéfalo/anatomía & histología , Panamá , Conducta SocialRESUMEN
Although eusociality evolved independently within several orders of insects, research into the molecular underpinnings of the transition towards social complexity has been confined primarily to Hymenoptera (for example, ants and bees). Here we sequence the genome and stage-specific transcriptomes of the dampwood termite Zootermopsis nevadensis (Blattodea) and compare them with similar data for eusocial Hymenoptera, to better identify commonalities and differences in achieving this significant transition. We show an expansion of genes related to male fertility, with upregulated gene expression in male reproductive individuals reflecting the profound differences in mating biology relative to the Hymenoptera. For several chemoreceptor families, we show divergent numbers of genes, which may correspond to the more claustral lifestyle of these termites. We also show similarities in the number and expression of genes related to caste determination mechanisms. Finally, patterns of DNA methylation and alternative splicing support a hypothesized epigenetic regulation of caste differentiation.
Asunto(s)
Fertilidad/genética , Regulación de la Expresión Génica , Proteínas de Insectos/genética , Isópteros/genética , Reproducción/genética , Conducta Social , Empalme Alternativo , Animales , Metilación de ADN , Epigénesis Genética , Perfilación de la Expresión Génica , Genoma , Proteínas de Insectos/metabolismo , MasculinoRESUMEN
The extent to which size constrains the evolution of brain organization and the genesis of complex behaviour is a central, unanswered question in evolutionary neuroscience. Advanced cognition has long been linked to the expansion of specific brain compartments, such as the neocortex in vertebrates and the mushroom bodies in insects. Scaling constraints that limit the size of these brain regions in small animals may therefore be particularly significant to behavioural evolution. Recent findings from studies of paper wasps suggest miniaturization constrains the size of central sensory processing brain centres (mushroom body calyces) in favour of peripheral, sensory input centres (antennal and optic lobes). We tested the generality of this hypothesis in diverse eusocial hymenopteran species (ants, bees and wasps) exhibiting striking variation in body size and thus brain size. Combining multiple neuroanatomical datasets from these three taxa, we found no universal size constraint on brain organization within or among species. In fact, small-bodied ants with miniscule brains had mushroom body calyces proportionally as large as or larger than those of wasps and bees with brains orders of magnitude larger. Our comparative analyses suggest that brain organization in ants is shaped more by natural selection imposed by visual demands than intrinsic design limitations.
Asunto(s)
Hormigas/fisiología , Abejas/fisiología , Evolución Biológica , Avispas/fisiología , Animales , Hormigas/anatomía & histología , Abejas/anatomía & histología , Tamaño Corporal , Encéfalo/anatomía & histología , Encéfalo/fisiología , Cognición , Tamaño de los Órganos , Avispas/anatomía & histologíaRESUMEN
The understanding of physiological and molecular processes underlying the sense of smell has made considerable progress during the past three decades, revealing the cascade of molecular steps that lead to the activation of olfactory receptor (OR) neurons. However, the mode of primary interaction of odorant molecules with the OR proteins within the sensory cells is still enigmatic. Two different concepts try to explain these interactions: the 'odotope hypothesis' suggests that OR proteins recognize structural aspects of the odorant molecule, whereas the 'vibration hypothesis' proposes that intra-molecular vibrations are the basis for the recognition of the odorant by the receptor protein. The vibration hypothesis predicts that OR proteins should be able to discriminate compounds containing deuterium from their common counterparts which contain hydrogen instead of deuterium. This study tests this prediction in honeybees (Apis mellifera) using the proboscis extension reflex learning in a differential conditioning paradigm. Rewarding one odour (e.g. a deuterated compound) with sucrose and not rewarding the respective analogue (e.g. hydrogen-based odorant) shows that honeybees readily learn to discriminate hydrogen-based odorants from their deuterated counterparts and supports the idea that intra-molecular vibrations may contribute to odour discrimination.
Asunto(s)
Abejas/fisiología , Odorantes , Olfato , Compuestos Orgánicos Volátiles/química , Animales , Condicionamiento Clásico , AprendizajeRESUMEN
The environment experienced during development can dramatically affect the brain, with possible implications for sensory processing, learning, and memory. Although the effects of single sensory modalities on brain development have been repeatedly explored, the additive or interactive effects of multiple modalities have been less thoroughly investigated. We asked how experience with multisensory stimuli affected brain development in the bumblebee Bombus impatiens. First, to establish the timeline of brain development during early adulthood, we estimated regional brain volumes across a range of ages. We discovered significant age-related volume changes in nearly every region of the brain. Next, to determine whether these changes were dependent upon certain environmental stimuli, we manipulated the visual and olfactory stimuli available to newly emerged bumblebee workers in a factorial manner. Newly emerged bumblebees were maintained in the presence or absence of supplemental visual and/or olfactory stimuli for 7 days, after which the volumes of several brain regions were estimated. We found that the volumes of the mushroom body lobes and calyces were larger in the absence of visual stimuli. Additionally, visual deprivation was associated with the expression of larger antennal lobes, the primary olfactory processing regions of the brain. In contrast, exposure to plant-derived olfactory stimuli did not have a significant effect on brain region volumes. This study is the first to explore the separate and interactive effects of visual and olfactory stimuli on bee brain development. Assessing the timing and sensitivity of brain development is a first step toward understanding how different rearing environments differentially affect regional brain volumes in this species. Our findings suggest that environmental factors experienced during the first week of adulthood can modify bumblebee brain development in many subtle ways.
Asunto(s)
Abejas/crecimiento & desarrollo , Vivienda para Animales , Plasticidad Neuronal , Animales , Encéfalo/crecimiento & desarrollo , Cuerpos Pedunculados/crecimiento & desarrollo , Tamaño de los Órganos , Estimulación Física , Olfato , Visión OcularRESUMEN
Worker polyphenisms in ants enable insightful analyses of neuronal underpinnings of division of labor, a crucial aspect of animal social organization. In the ant Pheidole dentata, which has a dimorphic worker caste, serotonin titer increases in the brain with age, modulating pheromonal recruitment communication and foraging, behaviors characteristic of mature individuals. Serotonin-immunoreactive (5HT-IR) neurons are found in the mushroom bodies (MB) and may modulate multi-sensory information processing associated with cues and social signals guiding task performance. The volume of this neuropil correlates with worker subcaste and age in P. dentata, but the role of structural variation in individual extrinsic MB neurons in division of labor in ants is poorly understood. We tested the hypothesis that branching complexity in a 5HT-IR calyx input neuron (CIN) in the MBs increases with age in minor workers of P. dentata in association with task repertoire expansion. We further predicted that major workers, which are defense specialists, have less elaborate CIN axonal arbors at any age in comparison to minor workers, which are task generalists. Contrary to our predictions, immunohistochemical and morphometric analyses revealed significantly greater CIN branching in both newly eclosed and mature major workers, and identified an effect of worker age on branching complexity only in majors. Our results indicate a modulatory role of the CIN in subcaste-specific behaviors and suggest behavioral specialization may be associated with the elaboration of specific MB neurons.
Asunto(s)
Hormigas/citología , Cuerpos Pedunculados/citología , Neuronas Serotoninérgicas/citología , Animales , Hormigas/fisiología , Conducta Animal , Cuerpos Pedunculados/fisiología , Neuronas Serotoninérgicas/fisiología , Conducta SocialRESUMEN
Color vision in honey bees (Apis mellifera) has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level by means of electrophysiological intracellular recordings of single neurons. Few visual neurons have been so far characterized in the lateral protocerebrum of bees. Therefore, the possible implication of this region in chromatic processing remains unknown. We performed in vivo calcium imaging of interneurons in the anterior optic tubercle (AOTu) of honey bees upon visual stimulation of the compound eye to analyze chromatic response properties. Stimulation with distinct monochromatic lights (ultraviolet [UV], blue, and green) matching the sensitivity of the three photoreceptor types of the bee retina induced different signal amplitudes, temporal dynamics, and spatial activity patterns in the AOTu intertubercle network, thus revealing intricate chromatic processing properties. Green light strongly activated both the dorsal and ventral lobes of the AOTu's major unit; blue light activated the dorsal lobe more while UV light activated the ventral lobe more. Eye stimulation with mixtures of blue and green light induced suppression phenomena in which responses to the mixture were lower than those to the color components, thus concurring with color-opponent processing. These data provide evidence for a spatial segregation of color processing in the AOTu, which may serve for navigation purposes.
Asunto(s)
Abejas/fisiología , Neuronas/fisiología , Lóbulo Óptico de Animales no Mamíferos/fisiología , Percepción Visual/fisiología , Animales , Calcio/metabolismo , Color , Percepción de Color/fisiología , Potenciales Evocados Visuales/fisiología , Estimulación Luminosa , Vías Visuales/fisiologíaRESUMEN
In honeybees, the conditioning of the proboscis extension response (PER) has provided a powerful tool to explore the mechanisms underlying olfactory learning and memory. Unfortunately, PER conditioning does not work well for visual stimuli in intact honeybees, and performance is improved only after antennal amputation, thus limiting the analysis of visual learning and multimodal integration. Here, we study visual learning using the PER protocol in harnessed bumblebees, which exhibit high levels of odor learning under restrained conditions. We trained bumblebees in a differential task in which two colors differed in their rewarding values. We recorded learning performance as well as response latency and accuracy. Bumblebees rapidly learned the task and discriminated the colors within the first two trials. However, performance varied between combinations of colors and was higher when blue or violet was associated with a high reward. Overall, accuracy and speed were negatively associated, but both components increased during acquisition. We conclude that PER conditioning is a good tool to study visual learning, using Bombus impatiens as a model, opening new possibilities to analyze the proximate mechanisms of visual learning and memory, as well as the process of multimodal integration and decision-making.
Asunto(s)
Aprendizaje por Asociación , Percepción de Color , Animales , Abejas , Toma de Decisiones , Modelos AnimalesRESUMEN
The honeybee Apis mellifera represents a valuable model for studying the neural segregation and integration of visual information. Vision in honeybees has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level using intracellular electrophysiological recordings of single neurons. However, our knowledge of visual processing in honeybees is still limited by the lack of functional studies of visual processing at the circuit level. Here we contribute to filling this gap by providing a neuroanatomical and neurophysiological characterization at the circuit level of a practically unstudied visual area of the bee brain, the anterior optic tubercle (AOTu). First, we analyzed the internal organization and neuronal connections of the AOTu. Second, we established a novel protocol for performing optophysiological recordings of visual circuit activity in the honeybee brain and studied the responses of AOTu interneurons during stimulation of distinct eye regions. Our neuroanatomical data show an intricate compartmentalization and connectivity of the AOTu, revealing a dorsoventral segregation of the visual input to the AOTu. Light stimuli presented in different parts of the visual field (dorsal, lateral, or ventral) induce distinct patterns of activation in AOTu output interneurons, retaining to some extent the dorsoventral input segregation revealed by our neuroanatomical data. In particular, activity patterns evoked by dorsal and ventral eye stimulation are clearly segregated into distinct AOTu subunits. Our results therefore suggest an involvement of the AOTu in the processing of dorsoventrally segregated visual information in the honeybee brain.