RESUMEN
OBJECTIVES: The aim of this study was to assess the performance of a patient-side blood test in determining neuter status in female cats. METHODS: Residual blood samples from female cats of unknown neuter status that were admitted to four cat adoption centres in the UK were tested for luteinising hormone (LH) using the Witness LH test (Zoetis). A positive LH test result indicated that the cat was neutered. Cats were assessed for evidence of a surgical scar suggestive of prior neutering; if none was found, an exploratory laparotomy was performed to confirm neuter status. The LH test performance was assessed (sensitivity, specificity, negative and positive predictive value). RESULTS: Two hundred and thirty-six cats had both LH test and exploratory laparotomy data. The specificity of the test in detecting neutered cats was 100% (95% confidence interval 96.2-99.9) and the sensitivity was 69% (95% confidence interval 59.3-76.8). The prevalence of neutered cats in this sample was 49%. The positive and negative predictive values were 1 and 0.77, respectively. CONCLUSIONS AND RELEVANCE: The Witness LH test correctly detected all unneutered cats and thus there were no false-positive results that incorrectly indicated a cat was neutered. This study therefore suggests that positive LH test results avoid the need to perform surgery to confirm neuter status. This has significant welfare benefits for cats as it provides a lower risk, faster and less traumatic alternative to surgery and, in the shelter setting, it will have a positive impact on the cost, speed of assessment and time to rehoming of cats.
Asunto(s)
Pruebas Hematológicas , Hormona Luteinizante/sangre , Ovariectomía/estadística & datos numéricos , Animales , Gatos , Femenino , Pruebas Hematológicas/estadística & datos numéricos , Pruebas Hematológicas/veterinaria , Vivienda para Animales , Sensibilidad y EspecificidadRESUMEN
Objectives The most common cause of lower urinary tract signs (LUTS) in cats under the age of 10 years is feline idiopathic cystitis (FIC). The prevalence of LUTS in the UK pet cat population is difficult to assess. This study used data collected prospectively to investigate the prevalence of, and risk factors for, owner-reported LUTS in a cohort of young pet cats. Methods Cat owners were recruited into a long-term longitudinal study and asked to complete questionnaires at specified age points for their cats. All cats were at least 18 months of age at the time of analysis. The prevalence of owner-reported LUTS at 18, 30 and 48 months of age was calculated, based on whether the owner had seen the cat urinating, and whether the cat had displayed one or more of the following clinical signs: dysuria, haematuria or vocalising during urination. A case-control study to investigate the risk factors for owner-reported LUTS in study cats at age 18 months was also conducted, using a multivariable logistic regression model. Results The prevalence of owner-reported LUTS in cats seen urinating by the owner was 4.3%, 3.8% and 6.0%, with 95% confidence intervals of 3.2-5.7%, 2.5-5.7% and 3.4-10.5% at ages 18, 30 and 48 months, respectively. An indoor-only lifestyle at the age of 18 months and a change in diet between the ages of 12 and 18 months were identified as risk factors for owner-reported LUTS at the age of 18 months from the multivariable model. No clear type of change in diet was identified in our sample of cats with LUTS. Conclusions and relevance The prevalence of owner-reported LUTS in a cohort of young pet cats was higher than the previously reported prevalence of LUTS in cats presenting to veterinary hospitals for LUTS or other reasons. A novel risk factor of change in diet between 12 and 18 months of age warrants further investigation.
Asunto(s)
Enfermedades de los Gatos/epidemiología , Propiedad , Animales , Animales Recién Nacidos , Estudios de Casos y Controles , Enfermedades de los Gatos/diagnóstico , Enfermedades de los Gatos/orina , Gatos , Estudios de Cohortes , Inglaterra/epidemiología , Femenino , Humanos , Masculino , Prevalencia , Factores de Riesgo , Encuestas y Cuestionarios , Urinálisis/veterinariaRESUMEN
Domestic cats have a unique breeding history and can be used as models for human hereditary and infectious diseases. In the current era of genome-wide association studies, insights regarding linkage disequilibrium (LD) are essential for efficient association studies. The objective of this study is to investigate the extent of LD in the domestic cat, Felis silvestris catus, particularly within its breeds. A custom illumina GoldenGate Assay consisting of 1536 single nucleotide polymorphisms (SNPs) equally divided over ten 1 Mb chromosomal regions was developed, and genotyped across 18 globally recognized cat breeds and two distinct random bred populations. The pair-wise LD descriptive measure (r(2)) was calculated between the SNPs in each region and within each population independently. LD decay was estimated by determining the non-linear least-squares of all pair-wise estimates as a function of distance using established models. The point of 50% decay of r(2) was used to compare the extent of LD between breeds. The longest extent of LD was observed in the Burmese breed, where the distance at which r(2) ≈ 0.25 was â¼380 kb, comparable to several horse and dog breeds. The shortest extent of LD was found in the Siberian breed, with an r(2) ≈ 0.25 at approximately 17 kb, comparable to random bred cats and human populations. A comprehensive haplotype analysis was also conducted. The haplotype structure of each region within each breed mirrored the LD estimates. The LD of cat breeds largely reflects the breeds' population history and breeding strategies. Understanding LD in diverse populations will contribute to an efficient use of the newly developed SNP array for the cat in the design of genome-wide association studies, as well as to the interpretation of results for the fine mapping of disease and phenotypic traits.
Asunto(s)
Gatos/genética , Cromosomas de los Mamíferos/genética , Haplotipos , Desequilibrio de Ligamiento , Alelos , Animales , Cruzamiento , Gatos/clasificación , Mapeo Cromosómico , Frecuencia de los Genes , Estudio de Asociación del Genoma Completo , Humanos , Análisis de los Mínimos Cuadrados , Análisis por Micromatrices , Fenotipo , Filogenia , Polimorfismo de Nucleótido SimpleRESUMEN
Burmese is an old and popular cat breed, however, several health concerns, such as hypokalemia and a craniofacial defect, are prevalent, endangering the general health of the breed. Hypokalemia, a subnormal serum potassium ion concentration ([K(+)]), most often occurs as a secondary problem but can occur as a primary problem, such as hypokalaemic periodic paralysis in humans, and as feline hypokalaemic periodic polymyopathy primarily in Burmese. The most characteristic clinical sign of hypokalemia in Burmese is a skeletal muscle weakness that is frequently episodic in nature, either generalized, or sometimes localized to the cervical and thoracic limb girdle muscles. Burmese hypokalemia is suspected to be a single locus autosomal recessive trait. A genome wide case-control study using the illumina Infinium Feline 63K iSelect DNA array was performed using 35 cases and 25 controls from the Burmese breed that identified a locus on chromosome E1 associated with hypokalemia. Within approximately 1.2 Mb of the highest associated SNP, two candidate genes were identified, KCNH4 and WNK4. Direct sequencing of the genes revealed a nonsense mutation, producing a premature stop codon within WNK4 (c.2899C>T), leading to a truncated protein that lacks the C-terminal coiled-coil domain and the highly conserved Akt1/SGK phosphorylation site. All cases were homozygous for the mutation. Although the exact mechanism causing hypokalemia has not been determined, extrapolation from the homologous human and mouse genes suggests the mechanism may involve a potassium-losing nephropathy. A genetic test to screen for the genetic defect within the active breeding population has been developed, which should lead to eradication of the mutation and improved general health within the breed. Moreover, the identified mutation may help clarify the role of the protein in K⺠regulation and the cat represents the first animal model for WNK4-associated hypokalemia.
Asunto(s)
Gatos , Modelos Animales de Enfermedad , Hipopotasemia/genética , Proteínas Serina-Treonina Quinasas/genética , Animales , Estudios de Casos y Controles , Análisis Mutacional de ADN , Canales de Potasio Éter-A-Go-Go/genética , Femenino , Estudio de Asociación del Genoma Completo/veterinaria , Humanos , Hipopotasemia/patología , Masculino , Fenotipo , Potasio/sangre , Potasio/metabolismoRESUMEN
Registration of a cat with a veterinary practice is likely to be a critical factor for access to key preventative medicine. A cross-sectional study was conducted to collect data in the United Kingdom on the registration status of cats and potential explanatory variables. These data were also used to identify potential sources of bias associated with selecting controls from veterinary registered populations of cats due to differences between registered and unregistered cats. Cat owners reported that 13.6% (84/616) of their cats had not been registered with a veterinary practice since living at their current address. Multivariable logistic regression indicated that unregistered cats were significantly more likely than registered cats to be entire, to have not been vaccinated within the previous year, to be living in households in Northern Ireland and in households with an annual income <£10,000.(1) Whilst the neuter status and the vaccination status of the cat are likely to result from non-registration, the household location and annual income are factors that can be used to inform future interventions designed to increase the proportion of veterinary registered cats.
Asunto(s)
Bienestar del Animal , Hospitales Veterinarios , Propiedad/estadística & datos numéricos , Mascotas , Animales , Castración/veterinaria , Gatos , Estudios Transversales , Recolección de Datos , Femenino , Humanos , Masculino , Factores Socioeconómicos , Encuestas y Cuestionarios , Reino Unido , Vacunación/veterinariaRESUMEN
The aim of this study was to use fluorescence in-situ hybridisation (FISH) to search for the tissues and cell types important in survival and persistence of Mycoplasma haemofelis, "Candidatus Mycoplasma haemominutum" or "Candidatus Mycoplasma turicensis" in infected cats. A 16S rDNA probe for each species was applied to formalin-fixed, paraffin wax-embedded tissues sections collected from experimentally infected cats. Tissues (n = 12) were collected, at necropsy, from ten cats which had been infected with M. haemofelis, and one each with "Ca. M. haemominutum" and "Ca. M. turicensis". M. haemofelis specific hybridisation was present on red blood cells (RBCs) in all tissues from acutely infected cats, but not the majority of tissues from chronically infected cats. "Ca. M. haemominutum" specific hybridisation was present on scattered RBCs within the spleen and liver. Specific probe hybridisation was not detected in any of the "Ca. M. turicensis" infected tissues. Haemoplasmas were detected on the surface of RBCs only and not any other cell type. Additionally, FISH was limited by sensitivity and could not detect the lower numbers of organisms present in tissues of cats chronically infected with M. haemofelis. Occasional organisms were detected in cats acutely infected with "Ca. M. haemominutum" but not "Ca. M. turicensis".
Asunto(s)
Enfermedades de los Gatos/microbiología , Hibridación Fluorescente in Situ/métodos , Infecciones por Mycoplasma/veterinaria , Mycoplasma/aislamiento & purificación , Animales , Enfermedades de los Gatos/diagnóstico , Gatos , ADN Bacteriano/genética , ADN Ribosómico/genética , Femenino , Hígado/microbiología , Masculino , Mycoplasma/genética , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/microbiología , Organismos Libres de Patógenos Específicos , Bazo/microbiologíaRESUMEN
The aim of this study was to develop quantitative real-time (q)PCR assays to detect all known haemoplasma species, and a human housekeeping gene in order to demonstrate both successful DNA extraction from clinical samples and to test for sample inhibition, and to apply these qPCRs to human blood samples and blood smears. Sensitive and specific generic haemoplasma qPCR assays were developed to amplify haemoplasma species, as well as human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal amplification control. An optimized technique for extracting DNA from stained blood smears was also developed. These methods were applied to anonymized blood samples obtained from 100 human immunodeficiency virus (HIV)-infected South Africans and 920 UK patients undergoing haematological examination, and to 15 blood smears recruited from previous studies describing human haemoplasmosis. Human GAPDH levels were acceptable in all but three of the blood samples and all but two of the blood smears. The latter could have arisen due to DNA degradation due to the old age (over 35 years) of these smears. Haemoplasma infection was found in one HIV-infected South African, but the species could not be characterized due to the very low levels of DNA present. This report describes novel extraction and qPCR methodologies for haemoplasma screening. Previously reported human haemoplasmosis based on cytological diagnosis alone should be viewed with caution.
Asunto(s)
Técnicas Bacteriológicas/métodos , Sangre/microbiología , Infecciones por Mycoplasma/diagnóstico , Mycoplasma/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Animales , Gatos , Cartilla de ADN/genética , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Gliceraldehído-3-Fosfato Deshidrogenasas/genética , Infecciones por VIH/complicaciones , Humanos , Tamizaje Masivo/métodos , Mycoplasma/genética , Reacción en Cadena de la Polimerasa/normas , Estándares de Referencia , Sensibilidad y Especificidad , Sudáfrica , Reino UnidoRESUMEN
The aim of the present study was to characterize the antigenic specificity of the humoral immune response made by cats infected with the feline hemoplasma, Mycoplasma haemofelis. A crude M. haemofelis antigen preparation was prepared from red blood cells (RBCs) collected from a cat at the time of a high level of bacteremia. Plasma samples were collected from six cats before and after experimental infection with M. haemofelis, with regular sampling being performed from 15 to 149 or 153 days postinfection (dpi). Preinfection RBC membrane ghosts were prepared from these six cats and used to identify erythrocyte proteins that may have contaminated the M. haemofelis antigen preparation. The M. haemofelis antigen preparation comprised 11 protein bands. The immunodominant bands on Western blotting with infected cat plasma had molecular masses of 78, 68, 60, 48, and 38 kDa. Most cats (n = 5) had plasma antibody that reacted with at least one band (always including the one of 68 kDa) at 15 dpi, and all cats were seroreactive by 29 dpi. The maximum number of antibodies from an individual animal specific for an antigen was identified in plasma collected from 57 to 99 dpi. Contamination of the M. haemofelis antigen preparation with RBC membrane proteins was observed. The contaminating RBC proteins had molecular masses of from 71 to 72 kDa (consistent with band 4.2) and 261 and 238 kDa (consistent with spectrin), and these were recognized by all plasma samples. A range of M. haemofelis antigens is recognized by cats infected experimentally with the organism. These represent possible targets for immunoassays, but care must be taken to prevent false-positive results due to host protein contamination.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Enfermedades de los Gatos/inmunología , Infecciones por Mycoplasma/veterinaria , Mycoplasma/inmunología , Animales , Antígenos Bacterianos/química , Western Blotting , Enfermedades de los Gatos/microbiología , Gatos , Femenino , Epítopos Inmunodominantes/química , Epítopos Inmunodominantes/inmunología , Masculino , Peso Molecular , Infecciones por Mycoplasma/inmunología , Infecciones por Mycoplasma/microbiologíaRESUMEN
The aim of the study was to describe blood and tissue copy number distribution during Mycoplasma haemofelis infection and determine if sequestration of organisms in body tissues could explain blood copy number cycling in infected cats. Thirteen domestic-shorthaired cats were used. Blood samples were regularly collected, and at a differing time point post-infection for each cat, tissue samples also collected, for quantitative PCR (qPCR). Absolute haemoplasma copy numbers were calculated for all blood and tissue samples, as well as an estimation of the ratio of tissue haemoplasma copy number to that expected in the tissue if a positive qPCR result arose due to tissue blood supply alone. Cats with high or moderate M. haemofelis blood copy numbers at the time of tissue collection had fewer M. haemofelis copies in most tissues than expected due to the tissue blood supply alone; only splenic and lung tissues consistently contained more M. haemofelis. However tissues collected from cats at a time of very low M. haemofelis blood copy numbers, when putative copy number cycling nadirs were occurring, were usually qPCR negative. Hence no evidence of significant tissue M. haemofelis sequestration was found in this study to explain the copy number cycling reported with this feline haemoplasma species.
Asunto(s)
Bacteriemia/veterinaria , Enfermedades de los Gatos/microbiología , Infecciones por Mycoplasma/veterinaria , Mycoplasma/aislamiento & purificación , Animales , Bacteriemia/microbiología , Enfermedades de los Gatos/sangre , Gatos , ADN Bacteriano/sangre , ADN Bacteriano/aislamiento & purificación , Femenino , Hematócrito/veterinaria , Masculino , Mycoplasma/clasificación , Mycoplasma/genética , Infecciones por Mycoplasma/sangre , Infecciones por Mycoplasma/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Organismos Libres de Patógenos EspecíficosRESUMEN
Polycystic kidney disease (PKD) is the most prevalent inherited genetic disease in cats with Persian and Persian-related breeds predominantly affected. Diagnosis of PKD relied on ultrasound scanning until the recent development of the PKD gene test. However, gene testing has limitations as it will only identify the autosomal dominant form of PKD and not other forms of cystic kidney disease. Ultrasound scanning also has the advantage of being able to assess the severity and progression of disease in PKD affected cats. The aim of this study was to demonstrate the repeatability of ultrasound scanning in the detection of PKD and to assess progression of the disease over time. This study demonstrated 100% repeatability of ultrasound scanning in the detection of PKD and has also demonstrated progression of disease in 75% of PKD positive cats assessed over a 1-year period.
Asunto(s)
Enfermedades de los Gatos/diagnóstico por imagen , Riñón/anomalías , Enfermedades Renales Poliquísticas/diagnóstico por imagen , Enfermedades Renales Poliquísticas/veterinaria , Vejiga Urinaria/anomalías , Animales , Enfermedades de los Gatos/diagnóstico , Gatos , Progresión de la Enfermedad , Estudios de Factibilidad , Femenino , Riñón/diagnóstico por imagen , Masculino , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Ultrasonografía/veterinaria , Vejiga Urinaria/diagnóstico por imagen , Medicina VeterinariaRESUMEN
The aim of this study was to compare blood copy, haematological and glucose values between cats experimentally infected with either Mycoplasma haemofelis (Group HF: 10 cats), 'Candidatus M. haemominutum' (Group HM: 3 cats) or 'Candidatus M. turicensis' (Group TU: 3 cats). Blood samples were collected regularly up to 85 days post-infection (DPI) for haemoplasma real-time quantitative PCR, haematology, Coombs' testing and blood glucose measurement. Statistical analysis was performed using a general linear model (ANOVA) appropriate for a repeated measures experiment with significance set as P<0.05. Cats in Group TU had significantly lower blood copy numbers than cats in Group HF (P<0.001) and HM (P<0.001). All Group HF cats developed anaemia (often severe), macrocytosis and evidence of erythrocyte-bound antibodies whereas Groups HM and TU cats did not. Group HF had significantly lower PCVs, haemoglobin concentrations and red blood cell counts, and significantly higher mean cell volumes, than Groups HM and TU. In Group HF, erythrocyte-bound antibodies reactive at 4 degrees C (both IgM and IgG) appeared between 8 and 22 DPI and persisted for two to four weeks, whereas those reactive at 37 degrees C (primarily IgG) appeared between 22 and 29 DPI and persisted for one to five weeks. In most cats antibodies appeared after the fall in haemoglobin started. Although Group TU had significantly lower glucose concentrations than Groups HF (P=0.006) and HM (P=0.027), mean blood glucose concentrations remained within the reference range in all groups. This study demonstrates that M. haemofelis infection, in contrast to 'Candidatus M. haemominutum' and 'Candidatus M. turicensis' infection, can result in a severe macrocytic anaemia and the development of cold and warm reactive erythrocyte-bound antibodies.
Asunto(s)
Enfermedades de los Gatos/microbiología , Infecciones por Mycoplasma/veterinaria , Mycoplasma , Anemia Macrocítica/tratamiento farmacológico , Anemia Macrocítica/microbiología , Animales , Antibacterianos/uso terapéutico , Sangre/microbiología , Glucemia/análisis , Enfermedades de los Gatos/sangre , Enfermedades de los Gatos/tratamiento farmacológico , Enfermedades de los Gatos/fisiopatología , Gatos , Prueba de Coombs , ADN Bacteriano/sangre , ADN Bacteriano/genética , Infecciones por Mycoplasma/sangre , Infecciones por Mycoplasma/microbiología , Infecciones por Mycoplasma/fisiopatología , Reacción en Cadena de la Polimerasa/veterinaria , Especificidad de la Especie , Organismos Libres de Patógenos EspecíficosRESUMEN
A case series of nine domestic cats with culture-confirmed Alternaria species infection is presented, with conclusions drawn regarding signalment, clinical signs, treatment and outcome. Middle aged neutered males were over-represented and all presented with cutaneous lesions involving the extremities (nose, pinnae and digits). Lesions were mainly slow-growing, poorly circumscribed nodules or plaques but some also presented as non-healing wounds. A combination of surgical excision with adjunctive medical therapy appeared to be the most successful treatment option but long courses of medical therapy were generally required and recurrence was common.
Asunto(s)
Alternaria/aislamiento & purificación , Antifúngicos/uso terapéutico , Enfermedades de los Gatos/epidemiología , Micosis/veterinaria , Factores de Edad , Animales , Enfermedades de los Gatos/tratamiento farmacológico , Enfermedades de los Gatos/cirugía , Gatos , Terapia Combinada , Extremidades/microbiología , Extremidades/patología , Masculino , Micosis/tratamiento farmacológico , Micosis/epidemiología , Micosis/cirugía , Recurrencia , Factores SexualesRESUMEN
A study was carried out to determine the prevalence of feline immunodeficiency virus (FIV) within a population of cats entering 10 UK adoption centres run by Cats Protection. All cats entering the adoption centres during 2004 were tested for FIV using a rapid enzyme immunoassay antibody test. The overall prevalence of positive test results was 3.1% (95% confidence intervals (CI) 2.7-3.5%), whilst the prevalence at different adoption centres varied from 0.8% (95% CI 0.1-1.5%) to 6.7% (95% CI 4.9-8.5%). Results of the multivariable logistic regression analysis showed that male cats, stray/feral cats and cats in poor health were at a greater risk of testing positive for FIV than female cats, cats that were relinquished by an owner and cats that were in good/fair health, respectively. No evidence was found for an association between neuter status and FIV test results. This study may help to identify cats that are relinquished to rescue centres with an increased risk of FIV for routine FIV testing.
Asunto(s)
Crianza de Animales Domésticos/métodos , Anticuerpos Antivirales/sangre , Síndrome de Inmunodeficiencia Adquirida del Felino/epidemiología , Virus de la Inmunodeficiencia Felina/aislamiento & purificación , Animales , Antígenos Virales/inmunología , Gatos/sangre , Intervalos de Confianza , Ensayo de Inmunoadsorción Enzimática/veterinaria , Síndrome de Inmunodeficiencia Adquirida del Felino/sangre , Femenino , Estado de Salud , Virus de la Inmunodeficiencia Felina/inmunología , Masculino , Análisis Multivariante , Oportunidad Relativa , Prevalencia , Factores de Riesgo , Estudios Seroepidemiológicos , Reino UnidoRESUMEN
A case-control study was used to identify variables associated with the risk of mortality in cats housed at adoption centres. Multivariable logistic regression, based on retrospective data collected for 194 cases (cats that died or were euthanased) and 320 controls (cats that did not die) revealed an increased risk of mortality for cats admitted to adoption centres unneutered, in fair/poor health and cats born at adoption centres. Cats aged 7 weeks or less and cats aged over 7 years had an increased risk of mortality compared with cats of other ages. The risk of mortality decreased as the time in the adoption centre increased. Cats with disabilities (eg, blindness) had a higher mortality risk than cats without disabilities. Knowledge of these risk factors can inform intervention strategies aimed at reducing the risk of cat mortality at adoption centres.
Asunto(s)
Crianza de Animales Domésticos , Castración/veterinaria , Enfermedades de los Gatos/mortalidad , Gatos/fisiología , Estado de Salud , Factores de Edad , Animales , Estudios de Casos y Controles , Femenino , Modelos Logísticos , Masculino , Mortalidad , Análisis Multivariante , Estudios Retrospectivos , Factores de Riesgo , Factores de Tiempo , Reino UnidoRESUMEN
Feline haemoplasma infection can cause haemolytic anaemia. The natural method of transmission of haemoplasmas between cats is currently unknown but the nature of some of the risk factors for infection suggests that saliva may act as a mode of transmission. The aim of this study was to determine if Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (CMhm) DNAs could be amplified from saliva and salivary gland samples collected from haemoplasma-infected cats.
Asunto(s)
Enfermedades de los Gatos/diagnóstico , Infecciones por Mycoplasma/veterinaria , Mycoplasma/aislamiento & purificación , Reacción en Cadena de la Polimerasa/veterinaria , Saliva/microbiología , Glándulas Salivales/microbiología , Animales , Enfermedades de los Gatos/transmisión , Gatos , ADN Bacteriano/química , ADN Bacteriano/genética , Amplificación de Genes , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/transmisión , Reacción en Cadena de la Polimerasa/métodos , Sensibilidad y Especificidad , Especificidad de la Especie , Factores de TiempoRESUMEN
Partial sequences of the RNase P RNA gene (rnpB) were obtained from a number of hemoplasmas and other Mycoplasma species. Phylogenetic analysis of these sequences showed that all hemoplasmas were present within a single clade and were most closely related to Mycoplasma fastidiosum, similar to the results found with 16S rRNA gene phylogeny.
Asunto(s)
Proteínas Bacterianas/genética , Mycoplasma/clasificación , Mycoplasma/genética , Ribonucleasa P/genética , Animales , Sangre/microbiología , ADN Bacteriano/química , ADN Bacteriano/genética , Datos de Secuencia Molecular , Infecciones por Mycoplasma/microbiología , Filogenia , Análisis de Secuencia de ADN , Homología de SecuenciaRESUMEN
Autosomal-dominant polycystic kidney disease (AD-PKD) is the most prevalent inherited genetic disease of cats, particularly affecting Persians. Until recently the condition has been diagnosed by renal ultrasound screening. With the identification of the genetic mutation responsible for AD-PKD it is now possible to use advanced molecular techniques to screen for the disease. We have developed a rapid, sensitive and specific real-time PCR genotyping assay that can detect the single nucleotide polymorphism responsible for AD-PKD. Of 72 UK Persian and Exotic Shorthair cats submitted for AD-PKD ultrasound screening, 29 were found to have the disease, 41 were negative and 2 were equivocal. The recently published PCR-RFLP method showed the AD-PKD mutation to be present in all 29 diseased cats and absent in the 41 negative and 2 equivocal cats. Our real-time PCR genotyping assay was in complete agreement with the PCR-RFLP results. Of 600 blood or buccal swabs analysed from April 2005 to January 2006, 165 were found to be AD-PKD positive and 435 were negative, giving a prevalence of 27.5%. All 194 cats with AD-PKD were found to be heterozygous for the mutation.
Asunto(s)
Enfermedades de los Gatos/genética , Riñón Poliquístico Autosómico Dominante/genética , Riñón Poliquístico Autosómico Dominante/veterinaria , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo de Nucleótido Simple/genética , Animales , Gatos , Genotipo , Riñón Poliquístico Autosómico Dominante/diagnóstico por imagen , Riñón Poliquístico Autosómico Dominante/patología , Polimorfismo de Longitud del Fragmento de Restricción , Sensibilidad y Especificidad , Ultrasonografía , Reino UnidoRESUMEN
The purpose of this study was to investigate the effect of chronic feline immunodeficiency virus (FIV) infection, and efficacy of marbofloxacin treatment, on Mycoplasma haemofelis infection. Six cats chronically infected with FIV-Glasgow8 (Group X) and six FIV-free cats (Group Y) were infected with M. haemofelis on Day 0 by intravenous blood inoculation. From Day 0 until Day 86 post-infection (pi), blood samples were collected for M. haemofelis and FIV provirus quantitative real-time PCR and haematology. Three of the six cats in each of Groups X and Y were randomly selected to receive marbofloxacin treatment (2 mg/kg PO q24 h) from Day 16 to 43 pi, with the remaining cats being untreated controls with no antibiotic treatment. The M. haemofelis copy numbers and haematological data were compared between Groups X and Y, and between marbofloxacin-treated and control cats using a Mann-Whitney U-test. M. haemofelis infection was associated with development of macrocytic hypochromic anaemia. In some cats, marked variation in M. haemofelis copy number over time (>100,000-fold difference within 48 h in some cats) and/or cycling of copy number was seen. No correlation was found between FIV provirus copy number and M. haemofelis copy number or haematological variables. No significant effect of chronic FIV infection on M. haemofelis copy number kinetics or haematological changes due to M. haemofelis infection was found, other than MCHC (P=0.03). Marbofloxacin treatment was associated with a significant decrease in M. haemofelis copy number (P=0.002), although consistent clearance of infection was not demonstrated. This study reveals the presence of marked fluctuations in M. haemofelis copy number kinetics in vivo and a significant response to marbofloxacin antibiotic treatment.
Asunto(s)
Antibacterianos/uso terapéutico , Enfermedades de los Gatos/tratamiento farmacológico , Síndrome de Inmunodeficiencia Adquirida del Felino/complicaciones , Fluoroquinolonas/uso terapéutico , Infecciones por Mycoplasma/veterinaria , Mycoplasma/crecimiento & desarrollo , Quinolonas/uso terapéutico , Animales , Enfermedades de los Gatos/sangre , Enfermedades de los Gatos/microbiología , Gatos , Enfermedad Crónica , Recuento de Colonia Microbiana/veterinaria , Síndrome de Inmunodeficiencia Adquirida del Felino/sangre , Femenino , Masculino , Mycoplasma/efectos de los fármacos , Infecciones por Mycoplasma/sangre , Infecciones por Mycoplasma/tratamiento farmacológico , Infecciones por Mycoplasma/microbiología , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/veterinaria , Distribución Aleatoria , Factores de Tiempo , Resultado del Tratamiento , Carga ViralRESUMEN
The purpose of this study was to investigate the effect of chronic feline immunodeficiency virus (FIV) infection, and efficacy of marbofloxacin treatment, on 'Candidatus Mycoplasma haemominutum' infection. Six cats chronically infected with FIV-Glasgow8 (group A) and six FIV-free cats (group B) were infected with 'Candidatus M. haemominutum' on day 0 by intravenous inoculation of blood. From day 0 to 105 post-infection (pi), blood samples were collected for 'Candidatus M. haemominutum' and FIV provirus quantitative real-time polymerase chain reaction (PCR) and haematological examination. Three of the six cats in each of the groups were randomly selected to receive marbofloxacin treatment (2mg/kg PO SID) from day 49 to day 76 pi, with the remaining cats being untreated controls. Maximum 'Candidatus M. haemominutum' copy number was reached around day 30 pi. No overt cycling or marked variation in copy number was observed. No significant effect of FIV infection on 'Candidatus M. haemominutum' copy number kinetics or anaemia indices was found. No correlation was found between FIV provirus copy number and 'Candidatus M. haemominutum' copy number or haematological variables. Although marbofloxacin treatment was associated with a significant decrease in 'Candidatus M. haemominutum' copy number, the copy number plateaued during treatment, with no negative PCR results. Additionally, after termination of marbofloxacin treatment the copy numbers of the treated cats increased to reach levels similar to those of the untreated cats within 7-10 days. This study documents, for the first time, the infection kinetics and antibiotic responsiveness of 'Candidatus M. haemominutum' infection.