RESUMEN
BACKGROUND: The aim of the present study paper was to identify the role of reactive oxygen species (ROS) in apoptosis signaling mechanisms. We used for this purpose two ruthenium complex compounds based on that overproduce these reactive species by their metabolism thus manifesting their antitumor activity too. MATERIALS AND METHODS: In vivo studies were performed in Walker 256 carcinoma-bearing Wistar rats treated with two ruthenium (III) (Ru(III)) complexes with -fluoroquinolones norfloxacin and ofloxacin. The treatment started 7 days after tumor grafting. We assayed the dynamics of apoptosis by flow-cytometry and the biochemical oxidative status parameters. The biological samples used were serum and whole-tumor tissues; the results were compared to the untreated control group. RESULTS: The results showed an increase of apoptosis from 14.79% to 59.72% 14.79% to 59.72% in tumor cells treated with the most active combination, ruthenium complex with norfloxacin. We also noted an increase of the oxidative status and ROS production during treatment. CONCLUSION: The newly-synthesized complexes are less toxic and their activity is based on the induction of oxidative stress.
Asunto(s)
Apoptosis/efectos de los fármacos , Carcinoma 256 de Walker/tratamiento farmacológico , Quinolonas/administración & dosificación , Rutenio/administración & dosificación , Animales , Carcinoma 256 de Walker/metabolismo , Carcinoma 256 de Walker/patología , Citometría de Flujo , Humanos , Quinolonas/síntesis química , Quinolonas/química , Ratas , Especies Reactivas de Oxígeno/metabolismo , Rutenio/química , Transducción de Señal/efectos de los fármacosRESUMEN
Lonicera caerulea is a species of bush native to the Kamchatka Peninsula (Russian Far East) whose berries have been extensively studied due to their potential high antioxidant activity. The aim of our work was to investigate the in vivo effects of the antioxidant action of Lonicera caerulea berry extracts on the dynamics of experimentally-induced tumors. Our data showed that aqueous Lonicera caerulaea extracts reduced the tumor volume when administered continuously during the tumor growth and development stages, but augmented the tumor growth when the administration of extracts started three weeks before tumor grafting. Prolonged administration of Lonicera caerulaea berry extracts induced the antioxidant defense mechanism in the tumor tissues, while surprisingly amplifying the peripheral oxidative stress.