Disulfidptosis, A Novel Cell Death Pathway: Molecular Landscape and Therapeutic Implications.

Programmed cell death is pivotal for several physiological processes, including immune defense. Further, it has been implicated in the pathogenesis of developmental disorders and the onset of numerous diseases. Multiple modes of programmed cell death, including apoptosis, pyroptosis, necroptosis, and ferroptosis, have been identified, each with their own unique characteristics and biological implications. In February 2023, Liu Xiaoguang and his team discovered "disulfidptosis," a novel pathway of programmed cell death. Their findings demonstrated that disulfidptosis is triggered in glucose-starved cells exhibiting high expression of a protein called SLC7A11. Furthermore, disulfidptosis is marked by a drastic imbalance in the NADPH/NADP+ ratio and the abnormal accumulation of disulfides like cystine. These changes ultimately lead to the destabilization of the F-actin network, causing cell death. Given that high SLC7A11 expression is a key feature of certain cancers, these findings indicate that disulfidptosis could serve as the basis of innovative anti-cancer therapies. Hence, this review delves into the discovery of disulfidptosis, its underlying molecular mechanisms and metabolic regulation, and its prospective applications in disease treatment.

DNA Methylation Pattern of CALCA and CALCB in Extremely Premature Infants with Monochorionic Triplets after Single-Embryo Transfer.

Compared with full-term peers, premature infants are more likely to suffer from neonatal diseases and death. Variations in DNA methylation may affect these pathological processes. Calcitonin gene-related peptide (CGRP) plays a complex and diversified role in reproduction and chronic inflammation, and participates in the functional maintenance of vascular adaptation and trophoblast cells during pregnancy. Here, premature live births with single-chorionic triple embryos after single-embryo transfer were used as research objects, while full-term infants with double embryos and double-chorionic twins were used as controls. DNA was extracted from umbilical cord tissues for pyrosequencing to detect the methylation level of CpG island in CGRP promoter region. The average values of CGRP methylation in the umbilical cord tissues of very premature fetuses were higher than that of normal controls obtained from the databases. Immunofluorescence results showed that the expression of αCGRP was decreased in the blood vessel wall of the umbilical cord of monozygotic triplets, especially in death cases, while the ßCGRP had a compensatory expression. In conclusion, our findings suggest that hypermethylation of CGRP might be considered as an important cause of serious neonatal morbidities.

The Value of Relative Size in the Ultrasound Diagnosis of Follicular Thyroid Neoplasm.

PURPOSE: Ultrasonography as the first choice for thyroid nodules is still difficult to distinguish between solid follicular thyroid neoplasm (FTN) and solid nodular goiter (NG). We tried to investigate the value of relative size (M/S, M: the maximum diameter of target nodule, S: the maximum diameter of the largest of the remaining nodules) that may help to differentiate FTN from NG. METHODS: T test and chi-square test were used to retrospectively analyze the differences of the clinical and ultrasonographic characteristics between FTN and NG in 422 cases in our hospital. T test was used to analyze the difference of M/S value in the two kinds of nodules. ROC was used to evaluate the accuracy of M/S value in distinguishing the two. RESULTS: There were statistically significant differences in age, echogenicity, calcification, peripheral halo and blood supply between the two. The M/S value is not only significantly different in the two kinds of nodules but also can be used as a quantitative indicator to guide ultrasound diagnosis. ROC analysis showed that the cutoff point and AUC of M/S value were 1.94 and 0.709, respectively. CONCLUSION: In the ultrasound diagnosis of multiple thyroid nodules, the M/S value can better distinguish FTN and NG. We need to be aware of FTN when the M/S value of the nodule is greater than 2.

Local Differential Privacy Protection of High-Dimensional Perceptual Data by the Refined Bayes Network.

Although the Crowd-Sensing perception system brings great data value to people through the release and analysis of high-dimensional perception data, it causes great hidden danger to the privacy of participants in the meantime. Currently, various privacy protection methods based on differential privacy have been proposed, but most of them cannot simultaneously solve the complex attribute association problem between high-dimensional perception data and the privacy threat problems from untrustworthy servers. To address this problem, we put forward a local privacy protection based on Bayes network for high-dimensional perceptual data in this paper. This mechanism realizes the local data protection of the users at the very beginning, eliminates the possibility of other parties directly accessing the user's original data, and fundamentally protects the user's data privacy. During this process, after receiving the data of the user's local privacy protection, the perception server recognizes the dimensional correlation of the high-dimensional data based on the Bayes network, divides the high-dimensional data attribute set into multiple relatively independent low-dimensional attribute sets, and then sequentially synthesizes the new dataset. It can effectively retain the attribute dimension correlation of the original perception data, and ensure that the synthetic dataset and the original dataset have as similar statistical characteristics as possible. To verify its effectiveness, we conduct a multitude of simulation experiments. Results have shown that the synthetic data of this mechanism under the effective local privacy protection has relatively high data utility.