Effect of mussel polysaccharide on glucolipid metabolism and intestinal flora in type 2 diabetic mice.

BACKGROUND: Type 2 diabetes (T2D) mellitus is a major metabolic disease, and its incidence and lethality have increased significantly in recent years, making it a serious threat to human health. Among numerous previous studies, polysaccharides have been shown to alleviate the adverse effects of T2D, but there are still problems such as insufficient analysis and poor understanding of the mechanisms by which polysaccharides, especially those of marine origin, regulate T2D. METHODS: In this study, we used multiple allosteric approaches to further investigate the regulatory effects of mussel polysaccharides (MPs) on T2D and gut microbiota disorders in mice by identifying changes in genes, proteins, metabolites and target organs associated with glucolipid metabolism using an animal model of T2D fed with high-fat diets, and to explore the underlying molecular mechanisms. RESULTS: After MP intervention, serum levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and high-density lipoprotein cholesterol (HDL-C) were up-regulated, and blood glucose and lipid levels were effectively reduced in T2D mice. Activation of signaling molecules related to the upstream and downstream of the insulin PI3K/Akt signaling pathway reduced hepatic insulin resistance. The relative abundance of short-chain fatty acid (SCFA)-producing bacteria (including Akkermansia, Siraeum Eubacterium and Allobaculum) increased and harmful desulfurizing Vibrio decreased. In addition, the levels of SCFAs were increased. CONCLUSION: These results suggest that MP can increase SCFA levels by altering the abundance of intestinal flora, thereby activating the PI3K/Akt signaling pathway and exerting hypoglycemic effects. © 2023 Society of Chemical Industry.

Effect of a chitosan coating incorporating epigallocatechin gallate on the quality and shelf life of bighead carp (Aristichthys nobilis) fillets during chilled storage.

The objective of this study was to investigate the potential application of chitosan coatings incorporating epigallocatechin gallate (EGCG) for preserving fillets of bighead carp during chilled storage. The fillets were coated with acetic acid and glycerol, chitosan, and chitosan-EGCG, respectively, and the changes in their physicochemical, microbiological, and sensory characteristics during storage at 4 °C were determined. Notably, total volatile basic nitrogen, thiobarbituric-acid-reactive substances, and K value of chitosan-EGCG coated fillets sampled on day 15 were 48.04 %, 60.19 %, and 32.91 % lower than untreated fillets, respectively. Microbial enumeration suggested that the inclusion of EGCG significantly improved the inhibitory effect of pure chitosan coating on the proliferation of microorganisms. Furthermore, the chitosan-EGCG coated fillets also performed the best in terms of color, texture, and sensory analysis, and extended the shelf-life of the fillets for at least 6 days. A principal component analysis further confirmed the preserving effect of the chitosan-EGCG coating. Mantel test results suggested that the fillets' organoleptic characteristics strongly correlated with physicochemical and microbiological indicators. Overall, this work provides an effective protocol for food quality control and the extension of shelf life during chilled storage, and it clarifies the relationships between organoleptic characteristics and physicochemical and microbiological indexes.

Preparation and characterization of chitosan films incorporating epigallocatechin gallate: Microstructure, physicochemical, and bioactive properties.

Novel chitosan films incorporating epigallocatechin gallate (EGCG) were prepared and demonstrated the ideal physical and mechanical properties required of candidate food packaging materials alongside desirable antioxidant and antibacterial activity. Compared with traditional chitosan films, chitosan films incorporated with EGCG were thicker, had higher tensile strength and water solubility, and had lower elongation at break, moisture content, degree of swelling, and water contact angles. Although EGCG-containing films were slightly darker in color than pure chitosan films, they exhibited a greater inhibitory effect on light-induced oxidation with obviously improved UV-vis barrier capability and opacity. Scanning electron microscopy results suggested that EGCG-incorporated samples had a rougher surface structure. This was further confirmed by atomic force microscopy and indicated that the addition of EGCG facilitated the formation of protective barriers through the interaction between the film and food surface. FTIR spectroscopy confirmed that EGCG interacted with chitosan by intermolecular hydrogen bonding and effectively improved the thermal stability of chitosan films. Notably, the incorporation of EGCG significantly enhanced the antioxidant and antibacterial activity of chitosan films. Hence, chitosan films incorporating EGCG have potential applications in the food industry as a novel active packaging material, especially in preventing food oxidation and spoilage in perishable foods.

Cold plasma treated air improves the characteristic flavor of Dry-cured black carp through facilitating lipid oxidation.

In this study, the main reactive oxygen/nitrogen species (ROS/RNS) in cold plasma treated air (CPTA) were determined, and their effects on lipid oxidation, fatty acid composition and volatile profile in dry-cured black carp were investigated. Results showed that ozone (O3) and nitrogen dioxide (NO2) were the main ROS/RNS, both of which were in a few mg/m3 to tens mg/m3. Increased peroxide value (POV), thiobarbituric acid reactive substances (TBARS) and 4-hydroxy-2-nonenal (HNE) level indicated CPTA promoted lipid oxidation in samples. The contents of unsaturated fatty acids (UFA) especially polyunsaturated fatty acids (PUFA) in samples after CPTA exposure were decreased significantly. Volatile analysis by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) showed that CPTA exposure promoted the formation of characteristic volatile flavor compounds in dry-cured black carp via facilitating the oxidation of UFA. This work provides new ideas for the application of cold plasma in food processing.

Identification of changes in volatile compounds in dry-cured fish during storage using HS-GC-IMS.

Changes in the volatile components of dry-cured fish during storage at 4 ℃ and 25 ℃ were investigated by HS-GC-IMS fingerprinting combined with principal component analysis (PCA). Topographic plots were used to establish the fingerprints of flavor substances, and 32 signal peaks were identified that corresponded to 22 compounds, mainly aldehydes, ketones, alcohols and esters. During storage at 4 ℃, 3-methylbutanal, dimethyl ketone and hexanal were the main volatile components, while hexanal, 1-octen-3-ol and 3-methylbutanal were the main compounds of dry-cured fish stored at 25 ℃; butyl methyl ketone was only observed at 25 °C. In the early stage of storage, significant changes in octanal, 3-methylbutanal and ethyl acetate were found in dry-cured fish at both temperatures (P < 0.05) while heptanal, pentanal and butanal changed significantly in the late stage (P < 0.05). PCA clearly distinguished the samples in relation to their storage temperature and time. In addition, heat map clustering analysis further revealed the differences and similarities among different samples, and those results were in accordance with the PCA results.

The binding of key fishy off-flavor compounds to silver carp proteins: a thermodynamic analysis.

The binding of key fishy off-flavor compounds (KFOCs), heptanal, octanal, nonanal, and 1-octen-3-ol, to silver carp proteins (total myofibrillar protein, myosin, and actin) was studied. Myosin was the primary binding receptor for all four KFOCs, and it showed the strongest binding at neutral pH and at higher ionic strengths. Thermodynamic models were applied to evaluate the number of binding sites, the binding constant, and the Gibbs free energy for the binding of the KFOCs to myosin. Myosin had approximately 1.0 sites for binding with the three linear-chain aldehydes and about 1.6 sites for binding with 1-octen-3-ol. Moreover, myosin showed the highest affinity for 1-octen-3-ol, and both its binding constant and its number of binding sites with the three linear-chain aldehydes were negatively correlated with the chain length. For all four KFOCs, the trends of the Gibbs free energies were the opposite of those observed for the binding constant and the number of binding sites. These results may provide a theoretical basis for improving the deodorization efficiency of traditional surimi rinsing methods by adjusting the properties of the solutions used.

Extraction of oil from high-moisture tuna liver by subcritical dimethyl ether: feasibility and optimization by the response surface method.

The purpose of this paper is to explore the possibilities of subcritical dimethyl ether extraction (SDME) of oil from tuna liver with high-moisture content. The results showed that the oil and water were successfully co-extracted from the liver, and could be easily separated by centrifugation. In addition, the response surface method was employed to optimize the process parameters of SDME, including temperature/pressure, time and stirring speed. It was predicted that a temperature/pressure of 42/0.80 °C/MPa, time of 50 min and stirring speed of 925 rpm were the optimum within the experimental ranges, with an oil yield of 17.46 ± 0.23%. Furthermore, supercritical carbon dioxide extraction (SC-CO2) was studied comparatively. Only minor differences were observed between the oils extracted by SDME and SC-CO2, which indicated the high-quality of the SDME-oil. With no freeze-drying procedure and the relatively low pressure used in SDME, SDME could be a promising technique for extraction of marine fish liver oil.

Determination of the effects of different washing processes on aroma characteristics in silver carp mince by MMSE-GC-MS, e-nose and sensory evaluation.

The present study investigated the volatile compounds of silver carp mince and the effects of washing processes on the integral aroma characteristics and aroma-active compounds (AACs) of the mince. 57 volatile compounds were identified by monolithic material sorptive extraction (MMSE) and GC-MS analysis, and 13 volatile compounds with odor activity values greater than 1 (OAVs > 1) were further selected as AACs contributing primarily to the integral aroma profile of silver carp. Washing methods affected the overall aroma profiles of fish samples by washing away or facilitating the release of AACs but to different extents. Compared with water washing, washing with saline and weak alkaline solution removed more AACs. Washing with water three times (T2) exhibited a relatively stronger removal effect on most AACs compared to washing with water twice (T1). Washing with a high concentration of saline (T4) produced a strong removal effect on AACs. The results of sensory evaluation and electronic nose (e-nose) detection for distinguishing the aroma characteristics of different samples demonstrated good agreement with those obtained by OAV analysis.

Characterization of important odorants in steamed male Chinese mitten crab (Eriocheir sinensis) using gas chromatography-mass spectrometry-olfactometry.

Chinese mitten crab (Eriocheir sinensis) from Yangcheng Lake in Jiangsu Province is a popular species due to its unique pleasant aroma and intensive umami taste. In this study, odorants in steamed male E. sinensis were investigated using the headspace-monolithic material sorptive extraction technique coupled with gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A total of 74 volatile compounds were found, and the results of the GC-MS-O analysis, combined with odor activity values, showed that trimethylamine (fishy, ammonia-like odor), (Z)-4-heptenal (mushroom-like odor), and benzaldehyde (paint-like odor) were the important odorants (IOs) in all 4 of the edible parts of steamed male E. sinensis. Furthermore, heptanal (mushroom-like odor) was common to the abdomen, claw, and leg meat but was not found as the IO in the gonad. The abdomen meat also contained 3-methylbutanal (vegetable-like, grassy odor), while 2 additional IOs were found in claw meat (2-methylbutanal, which has a mushroom odor and 3-ethyl-2,5-dimethylpyrazine, which has a chocolate-like, musty odor). Another IO (2-nonanone, chocolate-like odor) was also found in leg meat, while (E)-2-nonenal (green, fruity odor) was the IO found exclusively in the gonad.

Characterization of odor-active compounds in cooked meat of farmed obscure puffer (Takifugu obscurus) using gas chromatography-mass spectrometry-olfactometry.

The volatile and odor-active compounds in cooked meat of farmed obscure puffer (Takifugu obscurus) were analyzed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). The volatile compounds were extracted by the simultaneous distillation-extraction (SDE) method, then separated and identified by GC-MS. Odor-active compounds in the SDE extract were characterized by GC-MS-O. A total of 68 volatile compounds were found, including 23 aldehydes, 10 alcohols, nine ketones, 17 N- or S-containing compounds and aromatics, three acids, three alkanes, and three esters. Of these, 31 odor-active compounds were detected and identified. Trimethylamine (fishy), octanal (grassy, leafy, green), (E)-2-octenal (roast, fatty), 1-octen-3-ol (fishy, fatty, mushroom, grassy), 2-ethyl-1-hexanethiol (cooked fish), (E,E)-2,4-octadienal (cooked meat, sweet), 2-acetylthiazole (meaty, roast, nutty, sulfur), 2-acetylpyrrole (nutty, walnut, bread) were identified as the key odorants in the cooked meat of farmed obscure puffer based on posterior intensity and time-intensity methods.