Identification of novel antioxidant collagen peptides for preventing and treating H2 O2 -induced oxidative stress in HepG2 cells through in vitro and in silico approaches.

BACKGROUND: Nowadays, the prevalence of oxidative stress-related chronic diseases is increasing. The identification of novel antioxidant collagen peptides to counteract oxidative stress for individuals' health has gained significant attention. RESULTS: In this study, collagen peptides with antioxidant activities were separated and identified by ion chromatography, reversed-phase high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. The identified antioxidant collagen peptides were further screened by molecular docking for Keap1-targeted peptide inhibitors and their theoretical interaction mechanisms were investigated. Four novel antioxidant collagen peptides, GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA, with high binding affinity to Keap1 were selected. Molecular docking results demonstrated that the putative antioxidant mechanism of the four antioxidant collagen peptides contributed to their blockage of Keap1-Nrf2 interactions. The results of antioxidant activity of the four antioxidant collagen peptides proved that IDGRPGPIGPA exerted a high scavenging capacity for DPPH and ABTS free radicals, while GPAGPpGPIG improved the resistance of cells to hydrogen peroxide-induced oxidative damage by promoting the activation of intracellular antioxidant enzymes and the production of reduced glutathione in human hepatoma (HepG2) cells. CONCLUSION: The antioxidant collagen peptides (GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA) will be developed as novel functional food for human health in the near future. © 2023 Society of Chemical Industry.

Gelatin-based anticancer drug delivery nanosystems: A mini review.

Drug delivery nanosystems (DDnS) is widely developed recently. Gelatin is a high-potential biomaterial originated from natural resources for anticancer DDnS, which can effectively improve the utilization of anticancer drugs and reduce side effects. The hydrophilic, amphoteric behavior and sol-gel transition of gelatin can be used to fulfill various requirements of anticancer DDnS. Additionally, the high number of multifunctional groups on the surface of gelatin provides the possibility of crosslinking and further modifications. In this review, we focus on the properties of gelatin and briefly elaborate the correlation between the properties and anticancer DDnS. Furthermore, we discuss the applications of gelatin-based DDnS in various cancer treatments. Overall, we have summarized the excellent properties of gelatin and correlated with DDnS to provide a manual for the design of gelatin-based materials for DDnS.

Applications of Gelatin in Biosensors: Recent Trends and Progress.

Gelatin is a natural protein from animal tissue with excellent biocompatibility, biodegradability, biosafety, low cost, and sol-gel property. By taking advantage of these properties, gelatin is considered to be an ideal component for the fabrication of biosensors. In recent years, biosensors with gelatin have been widely used for detecting various analytes, such as glucose, hydrogen peroxide, urea, amino acids, and pesticides, in the fields of medical diagnosis, food testing, and environmental monitoring. This perspective is an overview of the most recent trends and progress in the development of gelatin-based biosensors, which are classified by the function of gelatin as a matrix for immobilized biorecognition materials or as a biorecognition material for detecting target analytes.

Detection and kinetic simulation of animal hair/wool wastes pyrolysis toward high-efficiency and sustainable management.

Large quantities of solid wastes are produced each year in the leather industry. The considerable wastes generated exhibit tremendous application potential in terms of renewable energy sources and functional materials. Among them, animal hair/wool wastes possess high carbon content, which can be used sustainably and efficiently by using pyrolysis. Herein, the pyrolysis process of hair/wool wastes was investigated using TG-IR and Py-GC/MS, while the pyrolysis kinetic and thermodynamic were analyzed using "model-free" methods. The results showed that the hair/wool waste pyrolysis process can be divided into three stages: dehydration, devolatilization, and carbonization. The volatile products were mainly phenols (7.42%) and heterocyclic compounds (21.26%), which can be directly used as bio-energy (bio-gases and bio-oil) or converted to other useful chemical products. The kinetic parameters (Ea and A) calculated using the Flynn-Wall-Ozawa, Kissinger-Akahira-Sunose, and Kissinger methods indicated the complexity of the decomposition reactions, which was also confirmed by thermodynamic (ΔH, ΔG, and ΔS) calculation. Some suggestions have also been provided for the preparation of functional biochar with heteroatoms (i.e., N, O, and S) doping. These results not only provide a guide for designing the pyrolysis of hair/wool wastes but can also help develop a potential method to convert the hair/wool wastes into bioenergy to achieve sustainable development of the leather industry.

Pyrolysis kinetics behavior of solid leather wastes.

In the leather industry, considerable amounts of non-recyclable solid leather wastes (SLWs) are produced and accumulated in factories. In this work, the non-isothermal thermochemical analysis (TGA) test was used to analyse the thermal degradation behaviour of chromium-tanned leather shaving, which is one of the main SLWs. The pyrolysis experiments were carried out under nitrogen atmosphere from 30 to 800 °C at different heating rates of 5, 10, 15 and 20 °C/min. Three different kinetic models, including the Flynn-Wall-Ozawa (FWO), Kissinger-Akahira-Sunose (KAS) and Kissinger methods, were used to calculate the kinetic parameters. The activation energy values calculated by FWO and KAS methods were 391.79 and 348.77 kJ/mol, respectively. In consideration of the high HHV (14.15 MJ/kg) and carbon productivity (10.15%), SLWs could represent a potential candidate material for bioenergy production and carbon preparation. These results could be used for the design of thermochemical conversion processes utilizing SLWs as feedstock.

Lithiophilic-lithiophobic gradient interfacial layer for a highly stable lithium metal anode.

The long-standing issue of lithium dendrite growth during repeated deposition or dissolution processes hinders the practical use of lithium-metal anodes for high-energy density batteries. Here, we demonstrate a promising lithiophilic-lithiophobic gradient interfacial layer strategy in which the bottom lithiophilic zinc oxide/carbon nanotube sublayer tightly anchors the whole layer onto the lithium foil, facilitating the formation of a stable solid electrolyte interphase, and prevents the formation of an intermediate mossy lithium corrosion layer. Together with the top lithiophobic carbon nanotube sublayer, this gradient interfacial layer can effectively suppress dendrite growth and ensure ultralong-term stable lithium stripping/plating. This strategy is further demonstrated to provide substantially improved cycle performance in copper current collector, 10 cm2 pouch cell and lithium-sulfur batteries, which, coupled with a simple fabrication process and wide applicability in various materials for lithium-metal protection, makes the lithiophilic-lithiophobic gradient interfacial layer a favored strategy for next-generation lithium-metal batteries.

An in situ self-developed graphite as high capacity anode of lithium-ion batteries.

Graphite with a large inter-planar distance (0.357 nm) was obtained from pig bone. It delivered an improving specific capacity which increased continuously to 538 mA h g(-1) at 1 A g(-1) after 1000 cycles. With microscopic characterization, it has been found that the pig-bone-based graphite was exfoliated to graphene during the charge-discharge process.