Au nanoparticles as label-free competitive reporters for sensitivity enhanced fiber-optic SPR heparin sensor.

A label-free Au NPs-enhanced surface plasmon resonance (SPR) sensor was developed for the ultrasensitive detection of heparin based on competitive adsorption behavior of heparin and Au NPs on the poly (dimethyl-diallylammonium chloride) (PDDA)-modified optical fiber surface and the corresponding change in the resonance wavelength of SPR. Due to the high affinity between heparin and PDDA, the present senor shows good analytical performance with respect to heparin detection. Two obvious advantages of the proposed heparin sensor over other reported methods are: its much wider linear concentration range (10-6-10-10 g/mL) and lower limit of detection (0.0257 ng/mL). The analysis of heparin in serum demonstrated that the present sensor exhibited high sensitivity and selectivity. It should be noted that the sensing strategy takes advantage of a portable fiber-optic SPR sensing system and avoids the need for complex processes for labeled-Au NPs, and thus the present sensor promises to be a practical tool for the point-of-care monitoring of heparin.

Mercaptopyridine-Functionalized Gold Nanoparticles for Fiber-Optic Surface Plasmon Resonance Hg2+ Sensing.

As a highly toxic heavy metal ion, divalent mercuric ion (Hg2+) is one of the most widely diffused and hazardous environmental pollutants. In this work, a simple, portable, and inexpensive fiber-optic sensor based on surface plasmon resonance (SPR) effect was developed for Hg2+ detection, which takes advantage of 4-mercaptopyridine (4-MPY)-functionalized Au nanoparticles (Au NPs/4-MPY) as a signal amplification tag. Based on the coordination between Hg2+ and nitrogen in the pyridine moiety, we developed the sensor by self-assembling 4-MPY on Au film surfaces to capture Hg2+ and then introducing Au NPs/4-MPY to generate a plasmonic coupling structure with the configuration of nanoparticle-on-mirror. The coupling between localized SPR increased changes in SPR wavelength, which allowed highly sensitive Hg2+ sensing in aqueous solution. The sensor exhibited superior selectivity for Hg2+ detection compared with other common metal ions in water. The sensor's Hg2+ detection limit is 8 nM under optimal conditions. Furthermore, we validated the sensor's practicality for Hg2+ detection in tap water samples and demonstrated its potential application for environmental water on-site monitoring.

Dual Kretschmann and Otto configuration fiber surface plasmon resonance biosensor.

We present a dual-resonance fiber surface plasmon resonance (SPR) sensor for biological analysis. The sensing element was fabricated by sequentially sputtering layers of indium tin oxide (ITO) (100 nm thickness) and Au (35 nm thickness) on the surface of an optical fiber. The refractive index dispersion effect of ITO material led to resonances in the near infrared and visible wavelength regions. The refractive index of ITO is larger than the optical fiber in visible spectral area (400 to 733nm), such that the structure is a typical Kretschmann configuration surface plasmon resonance sensor. However, an Otto configuration is observed in the near infrared area (NIR) due to the ITO refractive index being smaller than the fiber core. We characterized the sensor performance by measuring bulk refractive index (RI) sensitivity in the two configurations, which were 1345 nm/RIU in the Kretschmann configuration and 1100 nm/RIU in the Otto configuration. In addition, this sensor was applied for real-time and label-free monitoring of the IgG/anti-IgG biomolecular interaction. As a robust and ultra-compact SPR sensor, which possesses wide detection range and is highly sensitive, this fiber SPR sensor can be applied for real-time biological analysis and monitoring.

Dual-channel fiber surface plasmon resonance biological sensor based on a hybrid interrogation of intensity and wavelength modulation.

We investigate an intensity and wavelength modulation combined plasmon resonance-based fiber-optic sensor technology. Composed of gold nanoparticles (GNPs) and sandwich configuration of Au/indium tin oxide (ITO)/Au film, two sensing regions are fabricated separately along with unclad portions of the fiber-optic probe. It can simultaneously monitor both the light intensity from the Au NP channel and the wavelength from the Au/ITO/Au film channel with a single detector. As the refractive index (RI) of the external environment changes, the transmission intensity and resonance wavelength in the two channels are modified, which provides an interrogation of intensity and wavelength modulation. The sandwich film structure is formed using magnetron sputtering technology, and the GNPs functioning as localized surface plasmon resonators are coated on a multimode optical fiber via the layer-by-layer method. The experimental results reveal that the RI sensitivities of the two sensing channels are 334.1% RIU?1 and 1963.2??nm/RIU, respectively. Based on the above sensing design, we conduct real-time and label-free monitoring of IgG/anti-IgG and Con A/RNase B biomolecular interaction. The resonant dips excited by different sensing modes make it more attractive as a multichannel surface plasmon resonance analysis technology, which is valuable in biological and life sciences research and rapid diagnostics.