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J Microbiol Methods ; 130: 129-132, 2016 11.
Artículo en Inglés | MEDLINE | ID: mdl-27623480

RESUMEN

The performance of 3 blood culture bottles (BACTEC Plus Aerobic/F, Plus Anaerobic/F, and Anaerobic Lytic/F) were analyzed with clinical specimens collected from 688 Emergency Department patients. A total of 270 strains belonging to 33 species were identified, with E. coli and S. aureus as the most frequently detected. Overall recovery rate (RR) of bacteria and yeast was equivalent in the Plus Aerobic/F vials (208 of 270 isolates; 77.0%) and Anaerobic Lytic/F vials (206 isolates; 76.3%) and significantly better than in the Plus Anaerobic/F vials (189 isolates; 70.0%). Median time to detection (TTD) was earliest with the Anaerobic Lytic/F vials (12.0h) compared with the Plus Aerobic/F (14.6h) and Plus Anaerobic/F vials (15.4h). Positivity rate (PR) was similar for Anaerobic Lytic/F vials (76.9%) and Plus Aerobic/F vials (76.5%), but better if compared with Plus Anaerobic/F vials (69.4%). The PR and TTD for the combination of Plus Aerobic/F with Anaerobic Lytic/F (94.5% and 12.3h, respectively) was significantly better than with Plus Aerobic/F with Plus Anaerobic/F (87.8% and 14.1h).


Asunto(s)
Bacterias/aislamiento & purificación , Cultivo de Sangre/métodos , Sangre/microbiología , Levaduras/aislamiento & purificación , Aerobiosis , Anciano , Anaerobiosis , Bacteriemia/microbiología , Bacterias/crecimiento & desarrollo , Bacterias/patogenicidad , Infecciones Bacterianas/microbiología , Medios de Cultivo/química , Servicio de Urgencia en Hospital , Escherichia coli/aislamiento & purificación , Escherichia coli/patogenicidad , Femenino , Fungemia/microbiología , Humanos , Italia/epidemiología , Masculino , Micosis/microbiología , Estudios Prospectivos , Sepsis/diagnóstico , Sepsis/epidemiología , Sepsis/microbiología , Staphylococcus aureus/aislamiento & purificación , Staphylococcus aureus/patogenicidad , Factores de Tiempo , Levaduras/crecimiento & desarrollo , Levaduras/patogenicidad
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