RESUMEN
Since its initial discovery in the Hunan province of China, genomic DNA of porcine circovirus 4 (PCV4) has been detected in pigs across multiple provinces in China, as well as in South Korea. However, the prevalence of porcine circovirus type 4 in Gansu Province, China, remains unknown. To address this gap, we undertook an extensive study where we gathered 121 clinical samples displaying diverse clinical manifestations from pig farms in Gansu Province between 2022 and 2023. Employing a real-time fluorescence quantification method, we identified the presence of PCV4 genome. Out of the 121 clinical samples analyzed, 13 samples tested positive for PCV4, resulting in a positive rate of 10.74% (13/121). This finding confirms the presence of PCV4 in pig farms within Gansu Province, China. Furthermore, we successfully sequenced and analyzed the complete genomes of two distinct PCV4 strains, comparing them with 60 reference sequences archived in the GenBank database. The results revealed a high nucleotide homology (98.2-98.8%) between the strains obtained in this study and the PCV4 reference strains, indicating a relatively low evolutionary rate of the PCV4 genome. Phylogenetic analysis revealed that two strains in this study belong to PCV4a and PCV4c. As far as we know, this study marks the inaugural report on the molecular identification and genomic attributes of PCV4 in Gansu Province, China, offering valuable insights for devising preventive and control strategies against this emerging virus.
Asunto(s)
Infecciones por Circoviridae , Circovirus , Enfermedades de los Porcinos , Porcinos , Animales , Filogenia , Circovirus/genética , Infecciones por Circoviridae/epidemiología , Infecciones por Circoviridae/veterinaria , Enfermedades de los Porcinos/epidemiología , China/epidemiologíaRESUMEN
In order to further studying the relationship between the gastrointestinal endocrine cells and the hibernation of Chinese fire-bellied newt, histological techniques were used in this study to explore the distributed density and morphology of argentaffine cells in the digestive tract during hibernation and non-hibernation. The argentaffine cells could be observed throughout the digestive tract of Chinese fire-bellied newt during hibernation and non-hibernation and mainly distributed in the acinar epithelial cells, epithelial cells and intrinsic membrane. They had appeared in the shape of goblet, pyramid, ellipse, round, cone-shaped, spindle-shaped. The cytoplasm contains secretory granules at the basal regions with spherical nuclei in the basal regions. Most of them had processes in different directions, suggesting that the argentaffine cells have combined the function of endocrine and exocrine. The distribution density curves of argentaffine cells were generally wavelike during the two periods. It was highest in the body of stomach in hibernation, while in non-hibernation it was highest in the pyloric. Totally the density of argentaffine cells was higher in hibernation period than that in non-hibernation period. The results showed that the activity of the digestive tract was expectedly weakened during the hibernation, but the function of endocrine was actually strengthened.
Con el fin de promover el estudio de la relación entre las células endocrinas gastrointestinales y la hibernación del tritón vientre de fuego chino, fueron utilizados técnicas histológicas para explorar la densidad de distribuición y la morfología de las células argentafines en el tracto digestivo durante la hibernación y la no hibernación. Las células argentafines pudieron ser observadas en todo el tracto digestivo de tritón vientre de fuego chino durante la hibernación y no hibernación, y se distribuyeron principalmente en las células epiteliales acinares, células epiteliales y la membrana intrínseca. Aparecieron de forma caliciforme, piramidal, elíptica, redonda, cónica y de huso. El citoplasma contenía gránulos de secreción en las regiones basales con núcleos esféricos en esa región. La mayoría de ellos tenía procesos en diferentes direcciones, lo que sugiere que las células argentafines combinaron la función del sistema endocrino y exocrino. Las curvas de densidad de distribución de las células argentafines fueron generalmente ondulante durante los dos períodos. Esta fue más alta en el cuerpo del estómago en estado de hibernación, mientras que en la no hibernación fue mayor en el píloro. En total, la densidad de las células argentafines era mayor en el periodo de hibernación. Los resultados mostraron que la actividad del tracto digestivo se debilitó, como era de esperar, durante la hibernación, pero la función del sistema endocrino se fortaleció.