Hemophagocytic lymphohistiocytosis associated with brucellosis.

Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening immune disorder categorized as familial HLH or secondary HLH. Our case report describes a 63-year-old woman with epilepsy whose clinical signs were unremitting fever and altered consciousness. Primary abnormalities consisted of fever, splenomegaly, cytopenia, hypertriglyceridemia, hyperferritinemia and hemophagocytosis in the bone marrow. Results of blood next generation sequencing and blood culture confirmed Brucella infection. This report illustrates a sHLH case caused by Brucella melitensis infection. Here, we review the classification, clinical features, diagnostic methods, treatment regimens, differential diagnosis, and prognosis of HLH and brucellosis.

A Simple and Highly Sensitive Naked-Eye Analysis of EGFR 19del via CRISPR/Cas12a Triggered No-Nonspecific Nucleic Acid Amplification.

The mutation status of epidermal growth factor receptor (EGFR) exon 19 is of great importance for predicting sensitivity to tyrosine kinase inhibitors (TKIs) in the treatment of non-small-cell lung cancer (NSCLC). However, the development of simple, sensitive, and no-nonspecific amplification platforms for EGFR 19del detection in NSCLC remains a challenge. Herein, we developed a novel, simple, and highly sensitive naked-eye assay utilizing CRISPR/Cas12a-triggered no-nonspecific nucleic acid amplification (NAA) with rolling circle amplification (RCA) as a model for EGFR 19del detection. Typically, circular padlocks are designed to be the trans-cleavage substrate of Cas12a/crRNA and serve as templates for RCA. Since the target EGFR 19del induces robust trans-cleavage activity of the Cas12a/crRNA duplex, the surrounding circular padlocks are cleaved into random short linear fragments that are unable to initiate RCA, resulting in a colorless solution. However, in the absence of EGFR 19del, the inactivated Cas12a enzymes cannot cleave the circular padlocks, and they remain able to serve as templates to initiate RCA to generate long single-stranded DNA to further fold into G-quadruplex/hemin DNAzymes to catalyze the oxidation of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS2-), generating a color response that is obvious to the naked eye. As expected, this strategy with a detection limit as low as 20 fM exhibited robust selectivity and anti-interference ability. Moreover, this method was applicable for detecting EGFR 19del in real serum samples and showed high consistency with real-time quantitative polymerase chain reaction (qPCR) and sequencing results, providing a promising strategy for the early noninvasive diagnosis and guidance of clinical treatment for cancer.

Evaluation of Resources and Environment Carrying Capacity Based on Support Pressure Coupling Mechanism: A Case Study of the Yangtze River Economic Belt.

Resource and environmental carrying capacity (RECC) is an important basis for achieving sustainable urban development, and analysis of the relationship between regional resources and human activities is of great significance for sustainable regional development. Taking the Yangtze River Economic Belt (YREB) as the study area, this study establishes a framework for analyzing RECC based on the resource and environmental support capacity (RES) and the pressure on the resource and environment (REP), calculates the RES and REP of 110 cities in the YREB from 2009 to 2018, and analyzes the main constraints on RECC. The results show that (1) there are inter-regional imbalances in RECC within the study area, with cities that are more economically developed or at a higher administrative level usually having more severe problems with RECC. (2) The RES and REP indices of cities in the YREB show an overall increasing trend, but the relative growth rates of the RES and REP indices of cities at different levels differ. (3) The built-up area, green space in built-up areas, total gas supply, and length of sewage pipes are hindering factors for most cities to improve their RES. This study contributes to a comprehensive understanding of the current situation and changing trends of RECC in the YREB and can provide a reference for decision-making on sustainable development of the region's large river basin.

Rapid Detection of Clostridium tetani by Recombinase Polymerase Amplification Using an Exo Probe.

Tetanus is a potentially fatal public health illness resulted from the neurotoxins generated by Clostridium tetani. C. tetani is not easily culturable and culturing the relevant bacteria from infected wounds has rarely been useful in diagnosis; PCR-based assays can only be conducted at highly sophisticated laboratories. Therefore, a real-time recombinase polymerase amplification assay (Exo-RPA) was constructed to identify the fragments of the neurotoxin gene of C. tetani. Primers and the exo probe targeting the conserved region were designed, and the resulting amplicons could be detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in penetrating wounds. Tests of target-spiked serum and pus extract revealed that RPA is robust to interfering factors and has great potential for further development for biological sample analysis. This method has been confirmed to be reliable for discriminating between toxic and nontoxic C. tetani strains. The RPA assay dramatically improves the diagnostic efficacy with simplified device architecture and is a promising alternative to real-time PCR for tetanus detection.

Hierarchical Mo-doped CoP3 interconnected nanosheet arrays on carbon cloth as an efficient bifunctional electrocatalyst for water splitting in an alkaline electrolyte.

Design and fabrication of highly efficient and robust noble-metal-free bifunctional electrocatalysts for overall water splitting in alkaline media is challenging. Herein, we report a unique bifunctional electrocatalyst consisting of three-dimensional (3D) hierarchical Mo-doped CoP3 nanosheet arrays on carbon cloth (Mo-CoP3 NAs@CC) fabricated by a facile electrodeposition process and the subsequent PH3 plasma-assisted phosphorization. Benefiting from the hierarchical nanostructure and doping effect of Mo, the optimal Mo-CoP3-2@CC electrode demonstrates excellent HER and OER catalytic activity with an overpotential of 62 and 300 mV at 10 mA cm-2, respectively, and reasonable stability up to 20 h in 1.0 M KOH. Impressively, when Mo-CoP3-2@CC is used as both HER and OER electrodes in an alkaline electrolyzer, a current density of 10 mA cm-2 is achieved at a cell voltage of only 1.65 V, and the stable water-splitting current is maintained for 25 h, showing great promise for practical applications.

Supporting Sustainable Development of Water Resources: A Social Welfare Maximization Game Model.

Water can carry a boat but can also overturn it (human societal sustainable development). Governments faced aquatic ecosystem restoration and preservation challenges following the establishment of the United Nations Sustainable Development Goals. This paper proposes a social welfare maximization game model to analyze the dominant strategy equilibrium of enterprise-1 and enterprise-2 based on welfare maximization under the total sewage emission control policy. Under the aforementioned control policy, a stricter total sewage emission control of an enterprise corresponds to a lower enterprise output and a higher output of a competing enterprise; that is, the profit transfer effect occurs. When the government implements a relatively strict total sewage emission control policy for an enterprise, it is beneficial to reduce the amount of sewage emission from an enterprise but has no impact on the amount of sewage emission from a competing enterprise; however, the amount of sewage reduction of both enterprises will increase. If the government does not provide capital and technical support to enterprise-2, then enterprise-1 and enterprise-2 should implement total quantity control policies with different rigor degrees to avoid the one-size-fits-all phenomenon. To maximize social welfare, the government should adjust the total sewage emission control policy in time according to sewage stock changes and focus more on enterprises with insufficient capital and poor technical skills and provide financial and technical support.

Locational determinants of emissions from pollution-intensive firms in urban areas.

Industrial pollution has remained as one of the most daunting challenges for many regions around the world. Characterizing the determinants of industrial pollution should provide important management implications. Unfortunately, due to the absence of high-quality data, rather few studies have systematically examined the locational determinants using a geographical approach. This paper aimed to fill the gap by accessing the pollution source census dataset, which recorded the quantity of discharged wastes (waste water and solid waste) from 717 pollution-intensive firms within Huzhou City, China. Spatial exploratory analysis was applied to analyze the spatial dependency and local clusters of waste emissions. Results demonstrated that waste emissions presented significantly positive autocorrelation in space. The high-high hotspots generally concentrated towards the city boundary, while the low-low clusters approached the Taihu Lake. Their locational determinants were identified by spatial regression. In particular, firms near the city boundary and county road were prone to discharge more wastes. Lower waste emissions were more likely to be observed from firms with high proximity to freight transfer stations or the Taihu Lake. Dense populous districts saw more likelihood of solid waste emissions. Firms in the neighborhood of rivers exhibited higher waste water emissions. Besides, the control variables (firm size, ownership, operation time and industrial type) also exerted significant influence. The present methodology can be applicable to other areas, and further inform the industrial pollution control practices. Our study advanced the knowledge of determinants of emissions from pollution-intensive firms in urban areas.