RESUMEN
As a new soil amendment strategy, the effects of biochar application on soil microbial community have been reported in literature, but little information is available on the response of nitrogen-related microbial communities to biochar application in the reclaimed soil from coal-mining area. Through an outdoor pot experiment with reclaimed soil from coal-mining area of Huaibei, the effects of biochar and biochar compound fertilizer on nitrifier and denitrifier communities were investigated by real time PCR (qPCR) and terminal-restricted fragment length polymorphism (T-RFLP). There were five treatments: control (CK), NPK fertilizer (CF), biochar compound fertili-zer (BF), 2% biochar and NPK fertilizer (LB), 4% biochar and NPK fertilizer (HB). Results showed that compared with the CK, the treatments CF, BF, LB and HB significantly increased the abundance of ammonia oxidizing archaea (AOA), ammonia oxidizing bacteria (AOB), nirK and nirS genes of denitrifier. Compared with the CF treatment, BF, LB and HB significantly increased the abundance of AOB and nirK genes by 42.9%-82.1% and 33.5%-62.7%, respectively. Results of redundancy analysis showed that soil organic carbon, pH, NH4+-N and available potassium significantly affected AOB community structure, while soil organic carbon concentration, pH and NO3--N concentration significantly altered nirK-denitrifier community structure. Therefore, the application of biochar and biochar compound fertilizer could improve soil quality of the reclaimed soil from coal-mining area, and increase the abundances of nitrifier and denitrifier and alter community structure of AOB and nirK-denitrifier.
Asunto(s)
Minas de Carbón , Fertilizantes , Carbono , Carbón Orgánico , Carbón Mineral , SueloRESUMEN
Dysbiosis of the gastric microbiome is involved in the development of gastric cancer (GC). A number of studies have demonstrated an increase in the relative abundance of Lactobacillus in GC. In this review, we present data that support the overgrowth of Lactobacillus in GC from studies on molecular and bacterial culture of the gastric microbiome, discuss the heterogenic effects of Lactobacillus on the health of human stomach, and explore the potential roles of the overgrowth of Lactobacillus in gastric carcinogenesis. Further studies are required to examine the association between Lactobacillus and GC at strain and species levels, which would facilitate to elucidate its role in the carcinogenic process.
RESUMEN
A family of new dinuclear Cu(i) complexes with 1,4-bis(diphenylphosphino)butane (dppb) and functionalized 3-(2'-pyridyl)pyrazole mixed ligands has been synthesized and characterized. It is revealed that all these Cu(i) complexes include a [Cu2(dppb)2](2+) framework with the two Cu(i) atoms doubly bridged by a pair of dppb to generate a fourteen-membered Cu2P4C8 ring, and functionalized 3-(2'-pyridyl)pyrazole adopts a neutral chelating coordination mode without the N-H bond cleavage of the pyrazolyl ring. All these dinuclear Cu(i) complexes display a relatively weak low-energy absorption in CH2Cl2 solution, which is closely related to the variation of the Cu-N and Cu-P bonds caused by the substituent on the pyrazolyl ring. These dinuclear Cu(i) complexes are all emissive in solution and solid states at ambient temperature, which can be well modulated through structural modification of 3-(2'-pyridyl)pyrazole. It is shown that introduction of the trifluoromethyl group into the pyrazolyl ring is helpful for enhancing the luminescence properties of Cu(i) pyrazole phosphine complexes.