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This study aimed to explore the correlation between rhizosphere soil microorganisms of wild Arnebia euchroma and the content of medicinal components to provide guidance for the selection of the ecological planting base. The total DNA of rhizosphere soil microorganisms of wild A. euchroma was extracted, and the microbial community structure of rhizosphere soil microorganisms was analyzed by IlluminaMiseq high-throughput sequencing technology. The content of total hydroxynaphthoquinone pigment and β,β'-dimethylacrylalkannin in medicinal materials was determined by high-performance liquid chromatography(HPLC). The physicochemical pro-perties of rhizosphere soil of wild A. euchroma in main producing areas were determined, and the correlation of soil microbial abundance with index component content and soil physicochemical properties was analyzed by SPSS software. The results showed that the species composition of rhizosphere fungi and bacteria in A. euchroma from different habitats was similar at the phylum and genus levels, but their relative abundance, richness index(Chao1), and community diversity(Simpson) index were different. Correlation analysis showed that the content of available phosphorus in soil was positively correlated with the content of total hydroxynaphthoquinone pigment and β,β'-dimethylacrylalkannin, and the abundance of five fungal genera such as Solicoccozyma and six bacterial genera such as Pseudo-nocardia and Bradyrhizobium was positively correlated with the content of medicinal components in medicinal materials. The abundance of Bradyrhizobium was significantly positively correlated with the content of β,β'-dimethylacrylalkanin. The abundance of fungi such as Archaeorhizomyces was significantly positively correlated with the content of available phosphorus in rhizosphere soil, and Bradyrhizobium was significantly negatively correlated with soil pH. Therefore, the abundance of fungi and bacteria in the rhizosphere of A. euchroma has a certain correlation with the medicinal components and the physicochemical properties of the rhizosphere soil, which can provide a scientific basis for the selection of ecological planting bases in the later stage.
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Rizosfera , Microbiología del Suelo , Bacterias/genética , Fósforo , Suelo , BoraginaceaeRESUMEN
This study was aimed to observe the distribution of Mas-related G protein-coupled receptor A (MrgA) in cerebrospinal fluid (CSF)-contacting nucleus of normal rats and its expression in neuropathic pain, and to provide morphological evidence for CSF-contacting nucleus to participate in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of the sciatic nerve was made in Sprague-Dawley rats. The thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. The expressions of MrgA in the CSF-contacting nucleus were examined by double labeling with immunofluorescent staining. The results showed that on the 5th, 7th, 10th and 14th days, the values of MWT and TWL in CCI group were all lower than those in sham group (P < 0.05). MrgA was found to be distributed in CSF-contacting nucleus of normal rats; and the expression was markedly up-regulated in rats at the peak of neuropathic pain. Our data suggest that CSF-contacting nucleus may participate in neuropathic pain through the MrgA-mediated signaling pathway.
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Animales , Ratas , Neuralgia , Ratas Sprague-Dawley , Receptores Acoplados a Proteínas G/metabolismo , Proteína Estafilocócica A/metabolismo , Regulación hacia ArribaRESUMEN
This study established high-performance liquid chromatography(HPLC) fingerprints of Chinese medicines derived from Apocynum venetum and Poacynum pictum in Xinjiang and explored their composition differences with the combination of content determination, similarity analysis, cluster analysis and principal component analysis. The HPLC conditions included Phenomenex Kinetex C_(18) column(4.6 mm ×100 mm, 2.6 μm), acetonitrile-0.01% trifluoroacetic acid aqueous solution as mobile phase, gradient elution, flow rate of 0.6 mL·min~(-1), detection wavelength of 281 nm and column temperature of 25 ℃. The content of chlorogenic acid, quercetin-3-O-sophoroside, rutin, hyperin, isoquercitrin, trifolin and astragalin was determined in 31 batches of medicinal materials, and fingerprint research and chemometric analysis were performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004 A) and SPSS 21.0. In the Chinese Pharmacopoeia 2020, the quality of Apocyni Veneti Folium is controlled by character identification, microscopic identification, thin layer chromatography identification and quantitative determination of hyperin. There were 21 common peaks of A. venetum and P. pictum in the HPLC fingerprints, 5 of which were identified as chlorogenic acid, hyperin, isoquercitrin, trifolin and astragalin, with their content also determined. Except for 3 batches of medicinal materials, the similarity of other 28 batches was higher than 0.83, indicating good similarity. Two categories were formed in the cluster analysis based on content determination, which showed that some differences existed in similarities between different regions of Xinjiang. The medicinal materials were ranked by quality with principal component analysis, and the results indicated that the top 15 all came from northern Xinjiang. The quality difference of A. venetum and P. pictum had a correlation with the place of origin. This study provides a reference for the analysis and evaluation of A. venetum and P. pictum from different habitats and the selection of introduction and cultivation areas.
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Apocynum , China , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos , Medicina Tradicional ChinaRESUMEN
Objective:To understand the trend of Helicobacter pylori (Hp) resistance to clarithromycin and levofloxacin and to provide guidance for Hp eradication therapy. Methods:From January 2014 to December 2018, a total of 66 515 patients with gastrointestinal symptoms were enrolled in the First People's Hospital of Wenling. The patients were divided into the following groups: childhood (0 to 6 years old); juvenile (7 to 17 years old); youth (18 to 40 years old),middle age (41 to 65 years old),and old age (≥66 years old). All patients received gastroscopy, gastric mucosal biopsy, Hp culture and drug sensitivity test of clarithromycin and levofloxacin. Results:The Hp positive rate showed a significant downward trend in 2016, 2017 and 2018 (χ2=14.317, 47.079, 88.054, all P<0.05). The average resistance rate of Hp to clarithromycin from 2014 to 2018 was 22.72% (4 732/20 831) showing an increasing trend, but the increase was slower after 2017. The average resistance rate to levofloxacin was 30.55% (6 364/20 831), and the overall trend showed a sharp rise from 2015 to 2017 (χ2=38.383, 49.569, both P<0.05), and a significant decline was detected after 2017 (χ2=18.841, P<0.05). The resistance rate of Hp to levofloxacin in patients increased with age. The clarithromycin resistance rate first decreased and then increased with age, and the resistance rate in old age (32.52%, 763/2 346) was higher than that in youth (22.09%, 1 086/4 916) and middle age patients (21.21%, 2 854/13 458), and the differences were significant (χ2=991.071, 144.968, both P<0.05). The resistance rate of Hp rose from 12.73% (14/110) in juvenile to 43.31% (1 016/2 346) in old age (χ2=228.867, P<0.05). Conclusion:In recent years, the positive rate of Hp infection in Wenling area has a decreasing trend. Although the resistance rate of Hp to clarithromycin and levofloxacin has been rising slowly or decreasing, it is still at a high level. In the selection of Hp eradication program, the differences between patients in different age groups should be considered with particular attention on the minors.
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Farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily of ligand-activated transcription factors. As a metabolic regulator, FXR plays key roles in bile acid and cholesterol metabolism and lipid and glucose homeostasis. Therefore, FXR is a potential drug target for several metabolic syndromes, especially those related to lipidemia disorders. In the present study, we identified small molecule SIPI-7623, a derivative of an extract from Oriental wormwood (Artemisia capillaris), and found that it specifically upregulated the expression of cholesterol-7-alpha-hydroxylase (CYP7A1), downregulated the expression of sterol-regulatory element-binding protein 1c (SREBP-1c) in the liver, and inhibited the expression of ileal bile acid binding-protein (IBABP) in the ileum of rats. We found that inhibition of FXR by SIPI-7623 decreased the level of cholesterol and triglyceride. SIPI-7623 reduced the levels of cholesterol and triglyceride in in vitro HepG2 cell models, ameliorated diet-induced atherosclerosis, and decreased the serum lipid content on rats and rabbits model of atherosclerosis in vivo. Furthermore, SIPI-7623 decreased the extent of atherosclerotic lesions. Our resutls demonstrated that antagonism of the FXR pathway can be employed as a therapeutic strategy to treat metabolic diseases such as hyperlipidemia and atherosclerosis. In conclusion, SIPI-7623 could be a promising lead compound for development of drugs to treat hyperlipidemia and atherosclerosis.
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Animales , Humanos , Masculino , Conejos , Ratas , Artemisia , Química , Aterosclerosis , Quimioterapia , Genética , Metabolismo , Colesterol , Metabolismo , Colesterol 7-alfa-Hidroxilasa , Genética , Metabolismo , Medicamentos Herbarios Chinos , Hiperlipidemias , Quimioterapia , Genética , Metabolismo , Hipolipemiantes , Hígado , Metabolismo , Ratas Sprague-Dawley , Receptores Citoplasmáticos y Nucleares , Genética , Metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles , Genética , Metabolismo , Triglicéridos , MetabolismoRESUMEN
Farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily of ligand-activated transcription factors. As a metabolic regulator, FXR plays key roles in bile acid and cholesterol metabolism and lipid and glucose homeostasis. Therefore, FXR is a potential drug target for several metabolic syndromes, especially those related to lipidemia disorders. In the present study, we identified small molecule SIPI-7623, a derivative of an extract from Oriental wormwood (Artemisia capillaris), and found that it specifically upregulated the expression of cholesterol-7-alpha-hydroxylase (CYP7A1), downregulated the expression of sterol-regulatory element-binding protein 1c (SREBP-1c) in the liver, and inhibited the expression of ileal bile acid binding-protein (IBABP) in the ileum of rats. We found that inhibition of FXR by SIPI-7623 decreased the level of cholesterol and triglyceride. SIPI-7623 reduced the levels of cholesterol and triglyceride in in vitro HepG2 cell models, ameliorated diet-induced atherosclerosis, and decreased the serum lipid content on rats and rabbits model of atherosclerosis in vivo. Furthermore, SIPI-7623 decreased the extent of atherosclerotic lesions. Our resutls demonstrated that antagonism of the FXR pathway can be employed as a therapeutic strategy to treat metabolic diseases such as hyperlipidemia and atherosclerosis. In conclusion, SIPI-7623 could be a promising lead compound for development of drugs to treat hyperlipidemia and atherosclerosis.
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Animales , Humanos , Masculino , Conejos , Ratas , Artemisia , Química , Aterosclerosis , Quimioterapia , Genética , Metabolismo , Colesterol , Metabolismo , Colesterol 7-alfa-Hidroxilasa , Genética , Metabolismo , Medicamentos Herbarios Chinos , Hiperlipidemias , Quimioterapia , Genética , Metabolismo , Hipolipemiantes , Hígado , Metabolismo , Ratas Sprague-Dawley , Receptores Citoplasmáticos y Nucleares , Genética , Metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles , Genética , Metabolismo , Triglicéridos , MetabolismoRESUMEN
·AIM: To observe the clinical efficacy of intravitreal injection of ranibizumab combined with retinal laser photocoagulation for macular edema secondary to branch retinal vein occlusion (BRVO). ·METHODS: A total of 47 cases (47 eyes) of patients diagnosed of macular edema secondary to BRVO by fundus examination, fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) were included who were admitted to our hospital from August 2013 to March 2016. All patients were divided into two groups randomly, 47 of them finished the follow up. observation group composed of 25 patients(25 eyes) who underwent ranibizumab injection and after 2wk they received retinal laser photocoagulation for blocking venous reflux area, and control group consisting of 22 cases (22 eyes) were given intravitreal injection of ranibizumab alone. The best corrected visual acuity (BCVA), intraocular pressure (IOP), central macular retinal thickness ( CMT ) and the incidence of complications of patients from two groups were compared at the first month after the treatment. ·RESULTS:Observation group and control group's BCVA and CMT were significantly different at 1mo after treatment compared with before treatment (P<0.01), but IOP were not different (P>0.05). The BCVA at 1mo after treatment were not different between the two groups (P>0.05),the CMT were significantly different (P<0.01). · CONCLUSION: The clinical effect of ranibizumab injection combined with laser photocoagulation for blocking venous reflux area has advantages when compared with ranibizumab injection alone in the treatment of branch retinal vein occlusion, and laser photocoagulation does not stimulate macula with higher security.
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Purpose To detect Wnt5a and Wnt11 expression in lung cancer,to explore the relationship between their expression and the types of lung cancer,and to assess the relationships between their expression and clinicopathologic factors (such as gender,age,degree of cell differentiation,lymph node metastasis).Methods The 120 cases of lung cancer were selected as the experimental group.In addition,20 cases of normal lung tissue were selected as the control group.Immunohistochemistry EnVision method was used to detect the expression of Wnt5a and Wnt11.The results were analyzed by the statistical software.Results The positive expression rate of Wnt5a and Wnt1 1 was 36% and 38% in 84 cases of non-small cell lung carcinoma.While the expression rate of Wnt5a and Wnt11 were low (8.3% and 0,respectively)in 36 cases of small cell lung carcinoma.Both expression in non-small cell lung carcinoma was significantly higher than that of small cell carcinoma.The expression rate of Wnt11 in adenocarcinoma was higher (56%,while Wnt5a was 9%),and the expression rate of Wnt5a in squamous cell carcinoma was higher (50%,while Wnt11 was 25%).However,there was no significant difference between the two groups in the sex,age,differentiation and lymph node metastasis.Conclusion Both Wnt5a and Wnt11 expression was associated with lung cancer types.The positive expression rate of Wnt5a and Wnt11 in non-small cell lung carcinoma is significantly higher than that of small cell lung carcinoma.The expression level of Wnt5a is higher in squamous cell carcinoma,while Wnt11 is higher in adenocarcinoma.Both of their expression show no significant correlation with the lung cancer clinicopathological indicators (including the gender,age,degree of differentiation and lymph node metastasis in patients).
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<p><b>BACKGROUND</b>Prostate Imaging Reporting and Data System (PI-RADS) is a globally acceptable standardization for multiparametric magnetic resonance imaging (mp-MRI) in prostate cancer (PCa) diagnosis. The American College of Radiology revised the PI-RADS to address the limitations of version 1 in December 2014. This study aimed to determine whether the PI-RADS version 2 (PI-RADS v2) scoring system improves the diagnostic accuracy of mp-MRI of the prostate compared with PI-RADS v1.</p><p><b>METHODS</b>This retrospective study was approved by the institutional review board. A total of 401 consecutive patients, with clinically suspicious PCa undergoing 3.0 T mp-MRI (T2-weighted imaging + diffusion-weighted imaging + DCE) before transrectal ultrasound-guided biopsy between June 2013 and July 2015, were included in the study. All patients were scored using the 5-point PI-RADS scoring system based on either PI-RADS v1 or v2. Receiver operating characteristics were calculated for statistical analysis. Sensitivity, specificity, and diagnostic accuracy were compared using McNemar's test.</p><p><b>RESULTS</b>PCa was present in 150 of 401 (37.41%) patients. When we pooled data from both peripheral zone (PZ) and transition zone (TZ), the areas under the curve were 0.889 for PI-RADS v1 and 0.942 for v2 (P = 0.0001). Maximal accuracy was achieved with a score threshold of 4. At this threshold, in the PZ, similar sensitivity, specificity, and accuracy were achieved with v1 and v2 (all P > 0.05). In the TZ, sensitivity was higher for v2 than for v1 (96.36% vs. 76.36%, P = 0.003), specificity was similar for v2 and v1 (90.24% vs. 84.15%, P = 0.227), and accuracy was higher for v2 than for v1 (92.70% vs. 81.02%, P = 0.002).</p><p><b>CONCLUSIONS</b>Both v1 and v2 showed good diagnostic performance for the detection of PCa. However, in the TZ, the performance was better with v2 than with v1.</p>
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Adulto , Anciano , Anciano de 80 o más Años , Humanos , Masculino , Persona de Mediana Edad , Biopsia Guiada por Imagen , Imagen por Resonancia Magnética , Métodos , Próstata , Patología , Neoplasias de la Próstata , Diagnóstico , Curva ROC , Estudios Retrospectivos , Sensibilidad y EspecificidadRESUMEN
Morphology and molecular identification technology were used to identify 3 original plants of Fructus Elaeagni which was commonly used in Uygur medicine. Leaves, flowers and fruits from different areas were selected randomly for morphology research. ITS2 sequence as DNA barcode was used to identify 17 samples of Fructus Elaeagni. The genetic distances were computed by kimura 2-parameter (K2P) model, and the Neighbor-Joining (NJ) and Maximum Likelihood phylogenetic trees were constructed using MEGA5.0. The results showed that Elaeagnus angustifolia, E. oxycarpa and E. angustifolia var. orientalis cannot be distinguished by morphological characteristics of leaves, flowers and fruits. The sequence length of ITS2 ranged from 220 to 223 bp, the average GC content was 61.9%. The haplotype numbers of E. angustifolia, E. oxycarpa and E. angustifolia var. orientals were 4, 3, 3, respectively. The results from the NJ tree and ML tree showed that the 3 original species of Fructus Elaeagni cannot be distinguished obviously. Therefore, 3 species maybe have the same origin, and can be used as the original plant of Uygur medicineal material Fructus Elaeagni. However, further evidence of chemical components and pharmacological effect were needed.
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Código de Barras del ADN Taxonómico , Métodos , ADN de Plantas , Genética , ADN Espaciador Ribosómico , Genética , Contaminación de Medicamentos , Medicamentos Herbarios Chinos , Química , Clasificación , Elaeagnaceae , Clasificación , Genética , Frutas , Clasificación , Genética , Datos de Secuencia Molecular , Filogenia , Control de CalidadRESUMEN
Objective: In order to gain genetic polymorphism among different species, the genetic diversity of the plants in Fritillaria L. from Xinjiang was analyzed. Methods: Agarose gel electrophoresis and inter-simple sequence repeat (ISSR) analysis were used to investigate the genetic diversity and genetic relationship of the samples in Fritillaria L. from Xinjiang. Results: Total 185 bands were amplified and detected by 2% agarose gel electrophoresis, including 181 polymorphic bands with the percentage of polymorphic bands (PPB) at 97.84%. Twelve ISSR primers could significantly separate different species of plants in Fritillaria L. from Xinjiang with genetic similarity coefficient (GS) ranged from 0.37 to 0.80 revealed by UPGMA analysis. Ten species in Fritillaria L. from Xinjiang were clustered into four groups using 0.50 as the lowest GS. Conclusion: The results indicate that ISSR technique is useful in revealing the genetic diversity and genetic relationship among 10 species in Fritillaria L., which could provide a scientific basis for the resource collection and identification of the species in Fritillaria L. from Xinjiang.
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Objective To explore the relationship between esophagus cancer patients and both environmental and genetic factors,through analyzing the data on birth orders from esophagus cancer patients of Shanxi province.Methods Both Greenwood and Haldane methods on birth order were used to study the 1101 cases with esophagus cancer from Shanxi province.All the patients had received surgery and were diagnosed,by pathological evidence.First certificates of the patients were confirmed through the standard genetic epidemiologic investigation.Birth order was investigated on probands of the 1101 cases with esophagus cancer and their 44 siblings.Results Results form the Greenwood method showed that there was a tendency for cases with esophagus cancer in birth orders First to Third.However,the Haldane method showed that the results were quite different between actual value and the average theory value of 6A (6A(actual value)=17 118,(X)6A(average theory value) =19 290,X=∣6A-(X)6A∣/√V6A =7.63,X > 2) which suggested that the birth order had some effects on the occurrence of esophagus cancer.In addition,the actual value of 6A was lower than the theoretic average value,and the parents at younger productive age or baby at the first birth was easy to develop esophagus cancer.Conclusion Esophagus cancer was related with the birth order,especially at early order,which was not consistent with the national reports on esophagus cancer.Results from this study suggested that there were certain effects of environmental risk factors on esophagus cancer patients.
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Objective To analyze the different risks of cardia neoplasms in the immediate relatives of the cardia cancer patients,through a case-control study.Methods A case-control study was adopted on 772 cases and 772 controls,and relative risk (RR) were measured to compare the results from patemal or matrilineal groups.Results (1)Risk of the 1st grade kinship to the male cardia-cancer-patient group was obviously higher than that of the control group with RR=2.61 (95%CI:1.44-4.73,P<0.01).(2) The risks of both paternal (P<0.05) and matrilineal (P<0.05) in the male cardia-cancer-patients were obviously higher than that of the control groups while the risk of those male cardia-cancer-patients in the paternal was higher than that of the control group (P<0.05),so as the case for female patients in the matrilineal group (P<0.05).(3) Data from the 1st grade kinship of cardia-cancer-patient group showed that parents and siblings had a higher risk than the control group (P<0.05).(4) No significant genetic differences were found between the patemal of either the cancer group or the control group (P> 0.05),but statistical difference was observed that the risk of someone being the matrilineal of the cancer group was higher than that of the control group (P<0.05).Conclusion The risks of cardia-cancer were higher in the 1st grade kinship,which including parents,brothers,sisters,maternal grandmother,mother,and maternal aunt.It was suggested that prevention programs should be focused on both earlier detection and treatment of the patients.New strategy for cancer prevention also need to be further developed.
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Objective To investigate the prevalence of high risk human papillomavirus (HPV) genital infection and cervical cancer in adult women from Shenzhen.Methods Cluster sampling was used to investigate the prevalence of HPV infection and cervical cancer from women aged 20-59 years old living in Luohu,Futian,Nanshan,Longgang and Baoan districts in Shenzhen from April 2006 to April 2010.All women were detected for liquid-based cytology test (LCT) or Thinprep cytologic test (TCT)and high-risk HPV-DNA test with hybrid capture Ⅱ (HC-Ⅱ ).All women with ≥ASC-US by cytology and/or a positive HC- Ⅱ test were asked to return for colposcopy and four-quadrant biopsy.Endocervical curettage was performed.Pathological finding were used as the gold standard of the diagnosis of cervical intraepithelial neoplasia.Results 10 210 women were involved in the study and 10 017 of them having completed data.The overall positive rate of high-risk HPV-DNA was 16.29%.HPV positive rates in 20-,30-,35-,40-,45-,50-59 age groups were 17.37%,15.59%,16.33%,14.74%,17.16% and 17.98%,respectively.The curve of HPV infection rates in different age groups appeared a ‘W' shape.HPV infection rates in the 25-years-olds and 50-59 year-olds groups were significantly higher than the other age groups (x2=4.50,P=0.03 ).The overall prevalence rate of cervical intraepithelial lesions (CIN) was 7.52%,of which the prevalence rates of low-grade cervical intraepithelial lesions (CIN Ⅰ) was 5.32% high-grade cervical intraepithelial lesions (CIN Ⅱ/Ⅲ ) was 2.21%,cervical cancer was 0.12%.The prevalence of CIN Ⅰ was significantly higher than the CIN Ⅱ/Ⅲ (x2=134.15,P<0.001 ).The prevalence of cervical cancer in 45- age group was 0.12%,the highest.HPV infection rates increased with the grades of cervical lesions including women without CIN as 44.31%,in CIN Ⅰ as 70.73%,in CIN Ⅱ as 86.73%,and in CIN Ⅲ as 96.75% and in cancer as 100.00%.The HPV infection rates were different in districts (x2=17.81,P=0.03 ),with Futian and Luohu higher than those of Nanshan,Longgang and Baoan district.The prevalence rate of CIN in Baoan was lower than other districts.The CIN prevalence rates were not significantly different among the other districts of Shenzhen (x2=4.84,P=0.18).Conclusion The prevalence of cervical cancer was low in adult women living in Shenzhen,with cervical lesions still in the early stage.Prevention of HPV infection and treatment of CIN were the key points for the prevention of cervical cancer.
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Objective To evaluate the prognostic value of intima calcification detection with CT angiography (CTA) in carotid artery stenting (CAS).Methods Forty-five patients with anterior circulation stroke,admitted to our hospital from December 2008 to April 2011,were chosen in our study;the patients were divided into two groups,one without calcification (n=25) and the other with calcification (n=24) according to CT density.The calcium scores were calculated according to the method described by Agatston.Patients in the calcification group were subdivided into mild calcification group (n=4),medium calcification group (n=12) and severe calcification group (n=8).Remained percentage of stenosis after CAS in the none calcification group and calcification group were compared; correlation between Agatston scores of the calcification patients and remained percentage of stenosis after CAS was analyzed.Results Remained stenosis after CAS was noted in 47 of the 49 internal carotid arteries,and remained percentage of stenosis after CAS in the none calcification group was significantly lower than that in the calcification group (P=0.001); the severer the stenosis in the mild calcification group,medium calcification group and severe calcification group,the higher the remained percentage of stenosis after CAS (P<0.05).Degree of artery stenosis after CA S was closely correlated with Agatston scores (r=0.941,P=0.001).Conclusion CTA is reliable for diagnosing the carotid stenosis; evaluating carotid plaques with Agatston calcium scores is very useful for CAS.
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<p><b>OBJECTIVE</b>To compare three techniques of the transarticular screw placement in the lower cervical spine.</p><p><b>METHODS</b>The techniques of transarticular screw placements used by Takayasu (group A), Dalcanto (group B) and Klekamp (group C) were applied in twenty-four cervical specimens, among which 14 specimens were male and 10 specimens were female, ranging in age from 55 to 80 years, with an average of 68 years. The entry point of Takayasu technique was between the superior and median third of the vertical medial line of the lateral mass with 60 to 80 degrees caudal tilt and 0 degrees lateral tilt. Dalcanto technique started 2 mm caudal to the midpoint of lateral mass with 40 degrees caudal tilt and 20 degrees lateral tilt. Klekamp technique inserted the screws with the starting point being 1 mm medial and 1 mm inferior to the midpoint of the lateral mass with 40 degrees caudal tilt and 20 degrees lateral tilt. The splits of facets, the encroachment of the cervical nerve roots and vertebral arteries, and no-involving facets screws were observed and analyzed.</p><p><b>RESULTS</b>One hundred and ninety-two transarticular screws were implanted on both sides in twenty-four cervical cadavers, sixty-four for each group. There were twenty-five splits of inferior facets in group B, 2 splits in group C and none in group A. No superior facets were found to be broken in all cases. The difference of splits between group B and the other two groups was significant (Chi2AB = 31.07, P < .001; Chi2BC = 24.83, P < 0.01), while there was no statistical difference between group A and C (Chi2AC = 2.03, P > 0.05). Thirty-six screws encroached the vertebral arteries in group A, none in group B and C. There was significant statistical difference between group A and the other two groups (Chi2AB = 50.09, P < 0.01; Chi2AC = 50.09, P < 0.01). Forty anterior branches of lower cervical nerve roots were involved in group A, five in group B and three in group C. There were statistical differences between group A and the other two groups (Chi2AB = 41.98, P < 0.01; Chi2AC = 47-94, P < 0.01), and there was no statistical difference between group B and C. Sixteen posterior branches of lower cervical nerve roots were encroached in group A, 18 in group B and 14 in group C. There was statistical difference among the three groups (Chi2AB = 0.16, P > 0.05; Chi2AC = 0.17, 0.05; Chi2 = BC = 0.67, P >0.0 ). Although all screws went through facets in the study except for 5 in group A, there were no statistical differences between each groups Chi2AB k =3.33, P >0.05; X2A C =3.33, P> 0.05).</p><p><b>CONCLUSION</b>There are high risk of injury of anterior branch of cervical nerve root and vertebral artery if the screws are too long and the Takayasu's technique is used. However, the rate of facet split is high if the Dalcanto's technique is applied. Klekamp's technique is value to be applied.</p>
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Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Tornillos Óseos , Vértebras Cervicales , Cirugía General , Fijadores InternosRESUMEN
This study is to investigate the effect of ZL-004 on normal mouse and mice with leukopenia induced by chemotherapeutic agents. 5-Fluorouracil were administered intraperitoneally to mice to develop leucopenia, and the mice were treated with ZL-004. The number of peripheral leukocytes and the percentage of granulocyte in total WBC were examined. The results are that ZL-004 markedly raise peripheral blood leukocytes in the normal mice and the mice model of leukopenia. So, ZL-004 could protect mice against 5-fluorouracil damage and raise peripheral blood leukocyte. Features of bone marrow smears is myeloproliferative hyperactivity in the mice, particularly the matured granulocytic series were observed. The mechanism of ZL-004 is to act on the mouse bone marrow causing proliferation and differentiation.
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Animales , Femenino , Masculino , Ratones , Antineoplásicos , Farmacología , Células de la Médula Ósea , Biología Celular , Diferenciación Celular , Proliferación Celular , Fluorouracilo , Granulocitos , Biología Celular , Recuento de Leucocitos , Leucocitos , Biología Celular , Leucopenia , Patología , Ratones Endogámicos BALB C , Estructura Molecular , Pirrolidinonas , Química , Farmacología , Distribución AleatoriaRESUMEN
<p><b>OBJECTIVE</b>To detect the B cell activating factor (BAFF) and explore its significance in patients with warm autoimmune hemolytic anemia (WAIHA).</p><p><b>METHODS</b>The levels of serum soluble BAFF (sBAFF) and BAFF mRNA in peripheral blood mononuclear cells (PBMCs) in 30 healthy volunteers (control group) and 43 patients with WAIHA were measured by ELISA and real-time quantitative polymerase chain reaction (RT-qPCR) respectively.</p><p><b>RESULTS</b>The levels of serum sBAFF and BAFF mRNA in PBMCs in pretreatment group \[2311 (825 approximately 6523) ng/L and 884 (463 approximately 2346) ng/L\] was significanly higher than those in posttreatment group\[1205(358 approximately 5014) ng/L and 446(138 approximately 2699) ng/L\] and control group\[1128 (590 approximately 3201) ng/L and 341 (102 approximately 965) ng/L\] (both P < 0.01), the difference between the posttreatment group and control group was not statistically significant. There was no significant difference between therapy responsive and nonresponsive groups before treatment. There was a significant difference between the pre- and post-treatment resuets in responsive group (P < 0.01), but not in nonresponsive group (P > 0.05). The serum levels of sBAFF was positively correlated with the levels of the BAFF mRNA in PBMCs both in pre- and post therapy group (both P < 0.05).</p><p><b>CONCLUSION</b>The levels of serum sBAFF and BAFF mRNA in PBMCs are increased in patients with WAIHA, their dynamic alterations may contribute to the development of WAIHA.</p>
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Humanos , Anemia Hemolítica Autoinmune , Factor Activador de Células B , Genética , Interleucina-4 , Leucocitos Mononucleares , ARN Mensajero , GenéticaRESUMEN
<p><b>OBJECTIVE</b>To investigate the Bcl-2 protein and gene expression in diffuse large B-cell lymphoma (DLBCL), and analyze its correlation with immunosubtype and prognosis.</p><p><b>METHODS</b>Seventy-three cases of DLBCL were performed immunohistochemistry analysis with a panel of antibodies CD3, CD10, CD20, Bcl-6, Bcl-2 and MUM-1, and classified into germinal center B-cell (GCB) type and non-GCB type. Fluorescence in situ hybridization (FISH) was employed to detect bcl-2 gene expression in 57 cases with chromosome translocation t (14;18).</p><p><b>RESULTS</b>The percentages of tumor cells expressed CD10, Bcl-6, MUM-1 and Bcl-2 were 15.1%, 38.4%, 71.2% and 79.2%, respectively. 16 cases (21.9%) were GCB type and the rest (78.1%) were non-GCB type. 16 of 57 cases (28.1%) were t (14; 18), including 5 of GCB type (31.2%) and 11 of non-GCB type (68.2%). The expression of Bcl-2 protein was correlated with immunological subtype (P = 0.035), but not with survival time (P = 0.253). Between the t(14;18) positive and negtive groupes, there was significant difference for survival time (P = 0.022), but no difference for immunological subtype (P = 0.340). There was no correlation between Bcl-2 protein and t(14;18).</p><p><b>CONCLUSIONS</b>GCB type DBLBCL with expression of Bcl-2 protein had a poor prognosis. t(14; 18) positive BLBCL had poor prognosis. The expression of Bcl-2 protein and t(14; 18) are usually discordant.</p>
Asunto(s)
Humanos , Genes bcl-2 , Centro Germinal , Hibridación Fluorescente in Situ , Linfoma de Células B Grandes Difuso , PronósticoRESUMEN
Objective: To study the effect of genetic polymorphisms of cytochrome P4501A1 (CYP1A1), N-acetyltransferase 2 (NAT2) on the susceptibility to renal cancer. Methods: The genetic polymorphisms of CYP1A1 and NAT2 were examined in the renal cancer patients and controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. Results: The distribution of CYP1A1 (W/M) and NAT2 (intermediate) genotypes was significantly different between the renal cancer patients and controls (P<0.05). Individuals carrying CYP1A1 (W/M) or NAT2 (intermediate) genotypes had an increased risk for renal cancer(OR=2.487, 95% CI: 1.493-4. 142; OR=1.970, 95% CI: 1.128-3.442,respectively). Multivariate analysis showed increased risk for renal cancer patients carrying CYP1A1 (W/M) or NAT2 (intermediate) genotypes and those who smoke. Conclusion: The genotypes CYP1A1 (W/M) and NAT2 (intermediate) are the risk factors of renal cancer, and the 2 genotypes have a interactive effect and both have a joint effect with smoking.