RESUMEN
Patent foramen ovale (PFO) is an atrial septal deformity present in around 25% of the general population. PFO is associated with major causes of morbidity, including stroke and migraine. PFO appears to be heritable but genes involved in the closure of foramen ovale have not been identified. The aim of this study is to determine molecular pathways and genes that are responsible to the postnatal closure of the foramen ovale. Using Sprague-Dawley rat hearts as a model we analysed the dynamic histological changes and gene expressions at the foramen ovale region between embryonic day 20 and postnatal day 7. We observed a gradual loss of the endothelial marker PECAM1, an upregulation of the mesenchymal marker vimentin and α-smooth muscle actin, the elevation of the transcription factor Snail, and an increase of fibroblast activation protein (FAP) in the foramen ovale region as well as the deposition of collagen-rich connective tissues at the closed foramen ovale, suggesting endothelial-to-mesenchymal transition (EndMT) occurring during foramen ovale closure which leads to fibrosis. In addition, Notch1 and Notch3 receptors, Notch ligand Jagged1 and Notch effector HRT1 were highly expressed in the endocardium of the foramen ovale region during EndMT. Activation of Notch3 alone in an endothelial cell culture model was able to drive EndMT and transform endothelial cells to mesenchymal phenotype. Our data demonstrate for the first time that FO closure is a process of EndMT-mediated fibrosis, and Notch signalling is an important player participating in this process. Elucidation of the molecular mechanisms of the closure of foramen ovale informs the pathogenesis of PFO and may provide potential options for screening and prevention of PFO related conditions.
Asunto(s)
Tabique Interatrial/metabolismo , Foramen Oval Permeable/genética , Expresión Génica/genética , Animales , Tabique Interatrial/patología , Proteínas de Unión al Calcio/biosíntesis , Desarrollo Embrionario , Endopeptidasas , Endotelio/metabolismo , Endotelio/patología , Foramen Oval Permeable/patología , Gelatinasas/biosíntesis , Humanos , Péptidos y Proteínas de Señalización Intercelular/biosíntesis , Proteína Jagged-1 , Proteínas de la Membrana/biosíntesis , Mesodermo/metabolismo , Mesodermo/patología , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/biosíntesis , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/genética , Ratas , Receptor Notch1/biosíntesis , Receptor Notch3 , Receptores Notch/biosíntesis , Serina Endopeptidasas/biosíntesis , Proteínas Serrate-Jagged , Vimentina/biosíntesisRESUMEN
A 33-year-old man presented with an acute myocardial infarction (MI) after a recent long-haul flight. Angiography revealed thrombus in the right and circumflex coronary artery but no underlying atherosclerosis. Echocardiography revealed the presence of an atrial septal defect (ASD). In view of the recent flight, the presence of an ASD and occlusive thrombi in two coronary arteries, it was considered that the pathophysiological cause was paradoxical embolisation. Transcutaneous device closure of the ASD was implemented to prevent further catastrophic paradoxical embolic events.