Serotonin-Containing Cells in the Primary Organ of Hemopoiesis after Autologous Bone Marrow Transplantation.

Serotonin-containing cytostructures of mouse bone marrow after autotransplantation were studied using Falk-Hillarp luminescent histochemical method, and expression of antiapoptotic marker was determined by the immunohistochemical reaction. Autotransplantation of the bone marrow in mice led to an increase in the number of mast cells; in 40 and 120 min after autotransplantation, serotonin content in mast cells increased by 12 and 23%, respectively, and in hemopoietic cells of the bone marrow - by 24 and 78%, respectively, which affected the early stages of proliferation and differentiation of hemopoietic cells. The mitotic index in bone marrow smears at these terms increased by 3.8 and 4.5 times. According to myelogram data, the fraction of early undifferentiated forms (lymphoblasts, erythroblasts) in the primary organ of hemopoiesis (bone marrow) increased.

Effects of Bone Marrow Autotransplantation on the Bone Marrow Neurotransmitter Structures.

Morphological structures involved in local regulation of tissue processes and the neurotransmitters they produce are assumed to play an important role in the pathogenesis of oncological diseases. We studied the dynamics of neurotransmitter cell counts and distribution of catecholamines and serotonin in them and analyzed proliferative activity of hemopoietic cells in the bone marrow of mice after bone marrow autotransplantation. The bone marrow of male mice was studied by fluorescent and immunohistochemical methods after injection of the bone marrow from the same mouse into the caudal vein. The count of neurotransmitter cells and the cell proliferation index increased after bone marrow autotransplantation.

['Point of no return' in diabetic neuropathies: a dangerous delusion]. / 'Tochka nevozvrata' pri diabeticheskikh neiropatiiakh: opasnoe zabluzhdenie.

Polyneuropathy in patients with diabetes mellitus is manifested by a lesion of peripheral sensory, motor and autonomic nervous system. Different severity of damage of sensory, motor and autonomic fibers in typical and atypical forms of diabetic polyneuropathy, requires a differentiated approach to therapy, but not the rejection of its implementation. In an interdisciplinary consensus, consultations are held with physicians from different regions of the Russian Federation, and modern methods of diagnosing and assessing the severity of diabetic polyneuropathies, which determine the algorithm for treating patients, are discussed.

[Treatment of a neglect syndrome in movement rehabilitation of stroke patients]. / Osobennosti korrektsii sindroma neglekta pri osushchestvlenii dvigatel'noi reabilitatsii patsientov s polusharnym insul'tom.

AIM: To increase the efficiency and improvement of rehabilitation in patients with a neglect syndrome after stroke. MATERIAL AND METHODS: The results of treatment of 750 stroke patients, 396 women and 354 men, aged from 30 to 82 years, average age 63.4 years, were analyzed. An influence of cortexin on removal of the neglect syndrome, functional rehabilitation, increase in daily life activities and quality of life was studied. All patients were divided into two matched-controlled groups, receiving and not receiving cortexin. The functional rehabilitation was followed up using Barthel, Lindmark, Scandinavian and Merton and Sutton scales, MMSE and MoCA scales for assessment of cognitive functions, Sickness Impact Profile for quality of life. Moreover, the efficacy was evaluated by absence of signs of neglect syndrome. RESULTS: The combined rehabilitation with the use of cortexin helps to remove the neglect syndrome and improve the functional rehabilitation of stroke patients. CONCLUSION: Use of cortexin during rehabilitation in stroke patients with a neglect syndrome is justified and expedient for the increase of its efficiency that permits to recommend this drug for wide use in clinical practice.