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Background: B-cell leukemia/lymphoma 2 (Bcl-2) gene regulates carcinogenesis by inhibiting apoptosis. This study evaluated the association of Bcl-2 3'-untranslated regions (3' UTR) rs1564483 polymorphism and miR-296-3p with the development of breast and gastric cancers. Methods: A microarray analysis was performed on the Genomic Spatial Event (GSE)29431 and GSE161533 datasets for breast and gastric cancers. Blood samples were taken from 222 (111 patients and 111 controls) and 210 (84 patients and 126 controls) individuals for breast and gastric cancers, respectively. Genomic DNA was extracted from the blood samples and genotyping was performed using real-time polymerase chain reaction (RT-PCR), followed by examining the high-temperature melting curve. Statistical analysis was conducted to examine the potential correlation between the rs1564483 polymorphism and the risk of breast and gastric cancers concerning pathological characteristics. Results: The results of the microarray showed that the Bcl-2 gene was up-regulated in gastric cancer (logFC [log fold change]: 0.65, adjusted P < .05). Clinical outcome showed no notable relationship between the rs1564483 polymorphism and breast cancer risk; however, for gastric cancer, it identified a large difference between healthy controls and patients for an allelic frequency of rs1564483 (P ⩽ .001). Moreover, an assay of different models (dominant, recessive, and co-dominant) showed a significant association between the AG genotype between control and gastric cases (Pearson chi-square test, P = .046). In addition, the prevalence of the AG genotype was greater in persons under the age of 45 and in patients with H. pylori infection (P ⩽ .001). The AG genotype was not related to smoking, although the AA genotype was associated with increased cancer incidence in smokers (P ⩽ .001). Conclusions: In silico studies and calculations of the ΔG binding of micro ribonucleic acid (miRNA) hsa-miR-296-3p to the mutant and wild alleles of the rs15644833 single nucleotide polymorphism (SNP) have revealed that Bcl-2 mRNA expression in gastric cancer decreases, thus confirming the tumor suppressor role of the Bcl-2 gene.
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In this study, a facile synthesis of fluorescence carbon dots (CDs) from local oak apple (O-CDs) in the mountainous region of Zagros was performed through hydrothermal treatment. The characterization of O-CDs was carried out by SEM, TEM, FTIR, EDX, Mapping, lain scan, and AFM, respectively. In addition, the fluorescence of CDs was quenched by efavirenz with a linear concentration of 10 to 450 µM, associated with the limit of detection of 3 µM. Subsequently, the CDs were successfully applied for efavirenz probing in blood plasma environment. Supplementary Information: The online version contains supplementary material available at 10.1007/s10854-023-09929-z.
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In this study, using a thirsty plant extract and a simple hydrothermal method, a nano-probe was introduced to detect the phenobarbital based on fluorescence. Functional groups, particle size, surface morphology, and types of elements were identified using analysis such as FTIR, TEM, SEM, EDX, respectively. The excitation at 355 nm and emission intensity at 446 nm for nano-probe, the nano-probe shows that various parameters such as pH, temperature, and time were investigated for optimization conditions. After optimizing the factors affecting the sensor's response, a linear range between 0 and 750 µM with a detection limit of 5 µM was obtained. Then, the effect of interfering with other materials was investigated and finally, the ability of this sensor to measure the phenobarbital in real samples has been studied.
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A molecularly imprinted polymer (MIP) sensor was offered for nevirapine (NVP) analysis based on the electropolymerization of pyrrole (Py) on electrochemically reduced graphene oxide (ErGO) immobilized on a glassy carbon electrode (GCE). Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and atomic force microscope (AFM) were applied to characterize the proposed sensor (MIP/ErGO/GCE). The electrochemical operation of this sensor for NVP analysis was tested using differential pulse voltammetry (DPV) and cyclic voltammetry (CV) methods in an alkaline medium. The prepared MIP/ErGO/GCE exhibited better analytical performance than other modified electrodes toward NVP detection. The offered sensor depicted a linearity range between 0.005 µM and 400 µM with a limit of detection (LOD) of 2 nM under optimal conditions. Notably, the offered sensor illustrated excellent selectivity, good reproducibility, acceptable repeatability, and reliable long-term performance. These experiments depicted the constructed sensor as a favorable and good sensing element towards NVP monitoring in pharmaceutical and serum samples.