Drinking-water herbicide exposure in Indiana and prevalence of small-for-gestational-age and preterm delivery.

BACKGROUND: Atrazine and other corn herbicides are routinely detected in drinking water. Two studies on potential association of atrazine with small-for-gestational-age (SGA) and preterm birth prevalence found inconsistent results. Moreover, these studies did not control for individual-level potential confounders. OBJECTIVES: Our retrospective cohort study evaluated whether atrazine in drinking water is associated with increased prevalence of SGA and preterm birth. METHODS: We developed atrazine concentration time series for 19 water systems in Indiana from 1993 to 2007 and selected all births (n = 24,154) based on geocoded mother's residences. Log-binomial models were used to estimate prevalence ratios (PRs) for SGA and preterm delivery in relation to atrazine concentrations during various periods of the pregnancy. Models controlled for maternal demographic characteristics, prenatal care and reproductive history, and behavioral risk factors (smoking, drinking, drug use). RESULTS: Atrazine in drinking water during the third trimester and the entire pregnancy was associated with a significant increase in the prevalence of SGA. Atrazine in drinking water > 0.1 microg/L during the third trimester resulted in a 17-19% increase in the prevalence of SGA compared with the control group (< 0.1 microg/L). Mean atrazine concentrations over the entire pregnancy > 0.644 microg/L were associated with higher SGA prevalence than in the control group (adjusted PR = 1.14; 95% confidence interval, 1.03-1.24). No significant association was found for preterm delivery. CONCLUSIONS: We found that atrazine, and perhaps other co-occurring herbicides in drinking water, is associated with an increased prevalence of SGA, but not preterm delivery.

Acute and chronic toxicity of atrazine and its metabolites deethylatrazine and deisopropylatrazine on aquatic organisms.

Little is known about the toxicity of the atrazine (ATRZ) metabolites desethylatrazine (DEA) and deisopropylatrazine (DIA). We evaluated the acute and chronic toxicity of ATRZ, DEA, and DIA on the amphipods Hyalella azteca and Diporeia spp., and the unicellular algae Pseudokirchneriella subcapitata. In general, acute and chronic toxicity was ranked ATRZ > DEA > DIA. All 96-h median inhibition concentrations (IC(50)) were above concentrations found in the environment (>1,500 microg/L), and sensitivity was highest for the algae. When amphipods were exposed chronically (21 days), Diporeia was several orders of magnitude more sensitive compared to H. azteca. Neither ATRZ nor DEA altered H. azteca sex ratios. In conclusion, our results suggest that short-term exposures of these chemicals to algae and amphipods to concentrations routinely detected in surface waters are unlikely to be a cause of concern. However, the unexpected high sensitivity of Diporeia spp. to these herbicides deserves further attention considering the declining status of this amphipod in the Great Lakes basin.

Toxicity of soybean rust fungicides to freshwater algae and Daphnia magna.

Soybeans are intensively grown over large swaths of land in the Midwestern US. Introduction of the pathogenic fungus responsible for Soybean Rust (Phakopsora pachyrhizi) will likely result in a significant increase in the environmental load of strobilurin and conazole fungicides. We determined the toxicity of six such fungicides to the unicellular algae Pseudokirchneriella subcapitata and the aquatic invertebrate, Daphnia magna. We found that levels of concern of some fungicides were lower than annual average runoff concentrations predicted for Indiana. Our results suggest that pyraclostrobin and propiconazole, and to a lesser extent tebuconazole, may cause impacts to algae and daphnids in areas where soybeans are intensively grown. More studies are needed to describe the ecological effects of sublethal exposures to these fungicides, as well as monitoring environmental concentrations in watersheds where these fungicides are applied to soybeans.